TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCM3d10.yg.2.1
(683 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF322116.2|AF322116 Medicago sativa sucrose-phosphate sy... 306 e-100
gb|AY468362.2| Medicago sativa sucrose-phosphate synthase g... 122 1e-071
>gb|AF322116.2|AF322116 Medicago sativa sucrose-phosphate synthase mRNA, complete cds
Length = 3579
Score = 306 bits (662), Expect(2) = e-100
Identities = 125/152 (82%), Positives = 140/152 (92%)
Frame = +1 / +2
Query: 202 IWADIMRFFSNPRKPMILALARPDPKKNITTLVKAFGEHRELRNLANLTLIMGNRDVIDE 381
IW++IMRFF+NPRKP+ILALARPDPKKNITTLVKAFGE R LR LANLTLIMGNRD IDE
Sbjct: 1526 IWSEIMRFFTNPRKPVILALARPDPKKNITTLVKAFGECRPLRELANLTLIMGNRDGIDE 1705
Query: 382 MSSTNAAVLTSALKLIDKYDLYGQVAYPKHHKQSEVPDIYRLAARTKGVFINCALVEPFG 561
MSST+++VL S LKLIDKYDLYGQVAYPKHHKQS+VP+IYRLAA+TKGVF+N A++EPFG
Sbjct: 1706 MSSTSSSVLLSVLKLIDKYDLYGQVAYPKHHKQSDVPEIYRLAAKTKGVFVNPAIIEPFG 1885
Query: 562 LTLIEXXAYGLPMXXTRNGGPVXIHRVLDNGI 657
LTLIE AYGLPM T+NGGPV IHRVLDNG+
Sbjct: 1886 LTLIEAAAYGLPMVATKNGGPVDIHRVLDNGL 1981
Score = 203 bits (437), Expect(2) = 8e-062
Identities = 92/151 (60%), Positives = 102/151 (67%)
Frame = -1 / -1
Query: 653 PLSRTRCMXTGPPFRVXXMGRPYAXXSIKVSPNGSTNAQLIKTPFVLAAKR*ISGTSDCL 474
PLS TR + TGPPF V +G+P A SIKV PNGS A L KTPFV AA+R ISGTSDCL
Sbjct: 1977 PLSSTR*ISTGPPFLVATIGKP*AAASIKVRPNGSMIAGLTKTPFVFAARRYISGTSDCL 1798
Query: 473 WCLGYATCPYRSYLSINLSAEVKTAAFVLDISSMTSRFPMISVRFAKFLSSRCSPNALTR 294
WCLGYATCPYRSYLSINLS E +T VLDISS+ SR P+I+VR A L R SPNA T+
Sbjct: 1797 WCLGYATCPYRSYLSINLSTERRTEELVLDISSIPSRLPIINVRLASSLRGRHSPNAFTK 1618
Query: 293 VVIFFXXXXXXXXXXXXXXXFEKKRIISAQI 201
VV+FF K RIIS QI
Sbjct: 1617 VVMFFLGSGLARASITGLRGLVKNRIISDQI 1525
Score = 156 bits (335), Expect(2) = 1e-046
Identities = 71/154 (46%), Positives = 98/154 (63%)
Frame = -3 / -3
Query: 654 SIIKNPMYVXRPTISGXXHG*TICXXLNQGESKWFNQCTIDKNSFCSRS*TINIRNFRLL 475
+I++ PM + R +I H TI LN G++K FN ID+++ C R *TI RN RL
Sbjct: 1978 AIVEYPMNINRTSIFSSNHWQTISSCLN*GKTKRFNDSRIDEDTLCLRC*TIYFRNIRLF 1799
Query: 474 MVLGVCHLSI*IIFIN*LEC*SQNSCICA*HFINDITIPHDQCKIC*ISQFTMFTKCFD* 295
+V +CHL I I+ +N LE * +N CA*HFIN +++ H+ CK+ +S+ T F+KCF
Sbjct: 1798 VVFRICHLPIQIVLVNQLEY*EKNRRTCA*HFINSVSVTHN*CKVSKLSKRTAFSKCFHQ 1619
Query: 294 SSDILLRIRTSKSKNHWLPGV*EEAHYISPNRWI 193
S D+LLRI + KSK H L V +E HY+ PNR I
Sbjct: 1618 SCDVLLRIWSGKSKYHRLARVGKEPHYLRPNRRI 1517
Score = 92.3 bits (195), Expect(2) = 2e-023
Identities = 55/117 (47%), Positives = 66/117 (56%)
Frame = +2 / +3
Query: 302 KHLVNIVN*EI*QILH*SWGIVMSLMKCQAQMQLF*LQHSS*LINMIYMDKWHTPSTISN 481
KHL N V E LH* W LMKCQAQ+ LF Q+SS*L + I M KWH +T +N
Sbjct: 1626 KHLENAVLLESLLTLH*LWVTETELMKCQAQVLLFFSQYSS*LTSTICMGKWHILNTTNN 1805
Query: 482 LKFLIFIV*LREQKEFLSIVHWLNHLDSP*LRXXHMVYPXXXPEMVGLXTYIGFLIM 652
L FL +IV* R Q+ SI+ LN L P*LR MV +M L +IG+ M
Sbjct: 1806 LMFLKYIV*QRRQRVSSSILLSLNRLVLP*LRQLLMVCQWLLLKMEVLLIFIGYSTM 1976
Score = 74.8 bits (157), Expect(2) = e-100
Identities = 29/48 (60%), Positives = 34/48 (70%)
Frame = +1 / +2
Query: 10 DGFDLTMARKLRARIRRGVSCFGRXXXXMIAIPPGMEFSHIAPHDVDL 153
DGFD + RK+RARIRR VSC+GR + IPPGMEF HI P D D+
Sbjct: 1331 DGFDPVLERKIRARIRRNVSCYGRYMPRVAVIPPGMEFHHIVPQDGDI 1474
Score = 48.7 bits (100), Expect(2) = 8e-062
Identities = 25/46 (54%), Positives = 27/46 (58%)
Frame = -1 / -1
Query: 140 CGAI*LNSMPGGIAIIXXXXRPKQLTPRLILALSFRAIVRSKPSSR 3
CG I* NS+PGGI P QLT RLILA R+ SKPS R
Sbjct: 1461 CGTI*WNSIPGGITATRGMYLP*QLTLRLILARILRSSTGSKPSYR 1324
Score = 44.6 bits (91), Expect(2) = 1e-046
Identities = 19/48 (39%), Positives = 25/48 (52%)
Frame = -3 / -3
Query: 150 VNIMWCYMTKLHARRDCNHXXXXTTKAAHTTPYSCSEFPGHS*IKTII 7
+ ++W M +LH R + H +T AH TP CS F IKTII
Sbjct: 1471 ITVLWNNMMELHTRWNYRHARHVSTVTAHITPDPCSNFTLQHWIKTII 1328
Score = 35.4 bits (71), Expect(2) = 2e-023
Identities = 23/54 (42%), Positives = 30/54 (55%)
Frame = +3 / +1
Query: 9 *WF*SNYGPETQSKNKAWCELLWSXXXXYDCNPSWHGV*SYSTT*C*PRQ*GRK 170
*WF*S+ G SK++A CELL N + +GV SY +T * R * R+
Sbjct: 1330 *WF*SSAGA*NSSKDQA*CELLR*IHASRGGNSTGYGVPSYCSTRR*YRN*TRR 1491
>gb|AY468362.2| Medicago sativa sucrose-phosphate synthase gene, promoter region and
complete cds
Length = 8933
Score = 122 bits (260), Expect(6) = 1e-071
Identities = 50/59 (84%), Positives = 56/59 (94%)
Frame = +1 / +3
Query: 346 TLIMGNRDVIDEMSSTNAAVLTSALKLIDKYDLYGQVAYPKHHKQSEVPDIYRLAARTK 522
TLIMGNRD IDEMSST+++VL S LKLIDKYDLYGQVAYPKHHKQS+VP+IYRLAA+TK
Sbjct: 5373 TLIMGNRDGIDEMSSTSSSVLLSVLKLIDKYDLYGQVAYPKHHKQSDVPEIYRLAAKTK 5549
Score = 113 bits (242), Expect(5) = 1e-033
Identities = 50/78 (64%), Positives = 59/78 (75%)
Frame = -1 / -2
Query: 539 QLIKTPFVLAAKR*ISGTSDCLWCLGYATCPYRSYLSINLSAEVKTAAFVLDISSMTSRF 360
Q+ K FV AA+R ISGTSDCLWCLGYATCPYRSYLSINLS E +T VLDISS+ SR
Sbjct: 5566 QMDKVTFVFAARRYISGTSDCLWCLGYATCPYRSYLSINLSTERRTEELVLDISSIPSRL 5387
Query: 359 PMISVRFAKFLSSRCSPN 306
P+I+V A+ ++ S N
Sbjct: 5386 PIINV*KAQRVTYNRSDN 5333
Score = 92.3 bits (195), Expect(6) = 1e-071
Identities = 39/44 (88%), Positives = 41/44 (93%)
Frame = +1 / +2
Query: 214 IMRFFSNPRKPMILALARPDPKKNITTLVKAFGEHRELRNLANL 345
IMRFF+NPRKP+ILALARPDPKKNITTLVKAFGE R LR LANL
Sbjct: 5123 IMRFFTNPRKPVILALARPDPKKNITTLVKAFGECRPLRELANL 5254
Score = 69.3 bits (145), Expect(4) = 7e-023
Identities = 29/58 (50%), Positives = 38/58 (65%)
Frame = -3 / -1
Query: 519 CSRS*TINIRNFRLLMVLGVCHLSI*IIFIN*LEC*SQNSCICA*HFINDITIPHDQC 346
C R *TI RN RL +V +CHL I I+ +N LE * +N CA*HFIN +++ H+ C
Sbjct: 5546 CLRC*TIYFRNIRLFVVFRICHLPIQIVLVNQLEY*EKNRRTCA*HFINSVSVTHN*C 5373
Score = 48.7 bits (100), Expect(4) = 7e-023
Identities = 22/45 (48%), Positives = 30/45 (66%)
Frame = -3 / -2
Query: 345 KIC*ISQFTMFTKCFD*SSDILLRIRTSKSKNHWLPGV*EEAHYI 211
K+ +S+ T F+KCF S D+LLRI + KSK H L V +E HY+
Sbjct: 5254 KVSKLSKRTAFSKCFHQSCDVLLRIWSGKSKYHRLARVGKEPHYL 5120
Score = 45.5 bits (93), Expect(6) = 1e-071
Identities = 17/24 (70%), Positives = 20/24 (83%)
Frame = +1 / +1
Query: 10 DGFDLTMARKLRARIRRGVSCFGR 81
DGFD + RK+RARIRR VSC+GR
Sbjct: 4117 DGFDPVLERKIRARIRRNVSCYGR 4188
Score = 44.1 bits (90), Expect(6) = 1e-071
Identities = 16/23 (69%), Positives = 18/23 (78%)
Frame = +1 / +3
Query: 574 EXXAYGLPMXXTRNGGPVXIHRV 642
+ AYGLPM T+NGGPV IHRV
Sbjct: 6774 QAAAYGLPMVATKNGGPVDIHRV 6842
Score = 38.6 bits (78), Expect(6) = 1e-071
Identities = 14/19 (73%), Positives = 18/19 (94%)
Frame = +1 / +3
Query: 520 KGVFINCALVEPFGLTLIE 576
+GVF+N A++EPFGLTLIE
Sbjct: 6636 QGVFVNPAIIEPFGLTLIE 6692
Score = 34.5 bits (69), Expect(6) = 1e-071
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +1 / +1
Query: 94 MIAIPPGMEFSHIAPHDVDL 153
+ +PPGMEF HI P D D+
Sbjct: 4837 LAVVPPGMEFHHIVPQDGDI 4896
Score = 29.9 bits (59), Expect(5) = 1e-033
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = -2 / -2
Query: 76 QSSSHHALFLL*VSGP*LDQNHH 8
++SSH+A LL P LDQNHH
Sbjct: 4183 RNSSHYA*SLLVFYAPTLDQNHH 4115
Score = 29.0 bits (57), Expect(5) = 1e-033
Identities = 13/18 (72%), Positives = 13/18 (72%)
Frame = -1 / -2
Query: 575 SIKVSPNGSTNAQLIKTP 522
SIKV PNGS A L KTP
Sbjct: 6691 SIKVRPNGSMIAGLTKTP 6638
Score = 29.0 bits (57), Expect(5) = 1e-033
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = -1 / -2
Query: 647 SRTRCMXTGPPFRVXXMGRP 588
+ TR + TGPPF V +G+P
Sbjct: 6847 NHTR*ISTGPPFLVATIGKP 6788
Score = 26.7 bits (52), Expect(4) = 7e-023
Identities = 14/23 (60%), Positives = 15/23 (65%)
Frame = -1 / -1
Query: 77 PKQLTPRLILALSFRAIVRSKPS 9
P QLT RLILA R+ SKPS
Sbjct: 4184 P*QLTLRLILARILRSNTGSKPS 4116
Score = 24.9 bits (48), Expect(5) = 1e-033
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -1 / -1
Query: 140 CGAI*LNSMPGG 105
CG I* NS+PGG
Sbjct: 4883 CGTI*WNSIPGG 4848
Score = 24.9 bits (48), Expect(4) = 7e-023
Identities = 10/28 (35%), Positives = 14/28 (50%)
Frame = -1 / -3
Query: 617 PFRVXXMGRPYAXXSIKVSPNGSTNAQL 534
PF + + RP S VSP+ TN +
Sbjct: 8550 PFNITLLCRPPTSPS*SVSPHSPTNTTI 8467
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,413,569
Number of Sequences: 7669
Number of extensions: 69013
Number of successful extensions: 788
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 171396950
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)