TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCG22g05.yg.2.1
(681 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|AX776636.1| Sequence 5 from Patent WO02102841 84 2e-040
emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulatio... 84 2e-040
emb|AX776635.1| Sequence 4 from Patent WO02102841 82 2e-040
emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulatio... 82 2e-040
gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular tr... 139 2e-039
gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular tr... 52 9e-021
>emb|AX776636.1| Sequence 5 from Patent WO02102841
Length = 3339
Score = 83.5 bits (176), Expect(3) = 2e-040
Identities = 37/63 (58%), Positives = 45/63 (71%)
Frame = -3 / +3
Query: 268 ADNEKITSWLKRLEIAEDSAKGIEYLHTGCSPTIIHRDLKSSNILLDKNMRAKVADFGLS 89
A KI W RL IA +A+G+ YLHT ++IHRD+KSSNILLD++M AKVADFG S
Sbjct: 2298 ASKRKILDWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAKVADFGFS 2477
Query: 88 KPA 80
K A
Sbjct: 2478 KYA 2486
Score = 79.0 bits (166), Expect(3) = 2e-040
Identities = 35/74 (47%), Positives = 49/74 (66%)
Frame = -3 / +3
Query: 475 LADGREIAVKLLTNDSYQGIREFLNEVTLLSRIHHRHLVTFLGYSQQDGKNILVYEFMHN 296
L DG+E+AVK+ ++ S QG EF NE+ LLS I H +LV LGY + + ILVY FM N
Sbjct: 2088 LDDGQEVAVKVRSSTSTQGTXEFDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSN 2267
Query: 295 GTLKEHLRGADNEK 254
G+L + L G +++
Sbjct: 2268 GSLLDRLYGEASKR 2309
Score = 36.8 bits (74), Expect(3) = 2e-040
Identities = 16/28 (57%), Positives = 17/28 (60%)
Frame = -3 / +3
Query: 100 FGLSKPAVDGSHVSSIVRGTVGYLDPEY 17
F P S+VS VRGT GYLDPEY
Sbjct: 2472 FSKYAPQEGDSYVSLEVRGTAGYLDPEY 2555
>emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulation receptor kinase (nork gene), wild
type allele DMI2
Length = 3339
Score = 83.5 bits (176), Expect(3) = 2e-040
Identities = 37/63 (58%), Positives = 45/63 (71%)
Frame = -3 / +3
Query: 268 ADNEKITSWLKRLEIAEDSAKGIEYLHTGCSPTIIHRDLKSSNILLDKNMRAKVADFGLS 89
A KI W RL IA +A+G+ YLHT ++IHRD+KSSNILLD++M AKVADFG S
Sbjct: 2298 ASKRKILDWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAKVADFGFS 2477
Query: 88 KPA 80
K A
Sbjct: 2478 KYA 2486
Score = 79.0 bits (166), Expect(3) = 2e-040
Identities = 35/74 (47%), Positives = 49/74 (66%)
Frame = -3 / +3
Query: 475 LADGREIAVKLLTNDSYQGIREFLNEVTLLSRIHHRHLVTFLGYSQQDGKNILVYEFMHN 296
L DG+E+AVK+ ++ S QG EF NE+ LLS I H +LV LGY + + ILVY FM N
Sbjct: 2088 LDDGQEVAVKVRSSTSTQGTXEFDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSN 2267
Query: 295 GTLKEHLRGADNEK 254
G+L + L G +++
Sbjct: 2268 GSLLDRLYGEASKR 2309
Score = 36.8 bits (74), Expect(3) = 2e-040
Identities = 16/28 (57%), Positives = 17/28 (60%)
Frame = -3 / +3
Query: 100 FGLSKPAVDGSHVSSIVRGTVGYLDPEY 17
F P S+VS VRGT GYLDPEY
Sbjct: 2472 FSKYAPQEGDSYVSLEVRGTAGYLDPEY 2555
>emb|AX776635.1| Sequence 4 from Patent WO02102841
Length = 3378
Score = 81.7 bits (172), Expect(3) = 2e-040
Identities = 37/63 (58%), Positives = 44/63 (69%)
Frame = -3 / +1
Query: 268 ADNEKITSWLKRLEIAEDSAKGIEYLHTGCSPTIIHRDLKSSNILLDKNMRAKVADFGLS 89
A KI W RL IA + +G+ LHT P++IHRDLKSSNILLD++M AKVADFG S
Sbjct: 2317 ASKRKILDWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAKVADFGFS 2496
Query: 88 KPA 80
K A
Sbjct: 2497 KYA 2505
Score = 80.3 bits (169), Expect(3) = 2e-040
Identities = 35/74 (47%), Positives = 50/74 (67%)
Frame = -3 / +1
Query: 475 LADGREIAVKLLTNDSYQGIREFLNEVTLLSRIHHRHLVTFLGYSQQDGKNILVYEFMHN 296
L DG+E+AVK+ ++ S QG +EF NE+ LLS I H +LV LGY + + ILVY FM N
Sbjct: 2107 LDDGQEVAVKVRSSTSTQGTKEFDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSN 2286
Query: 295 GTLKEHLRGADNEK 254
G+L + L G +++
Sbjct: 2287 GSLLDRLYGEASKR 2328
Score = 36.8 bits (74), Expect(3) = 2e-040
Identities = 16/28 (57%), Positives = 17/28 (60%)
Frame = -3 / +1
Query: 100 FGLSKPAVDGSHVSSIVRGTVGYLDPEY 17
F P S+VS VRGT GYLDPEY
Sbjct: 2491 FSKYAPQEGDSYVSLEVRGTAGYLDPEY 2574
>emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulation receptor kinase (nork gene),
mutant allele nn1
Length = 3337
Score = 81.7 bits (172), Expect(3) = 2e-040
Identities = 37/63 (58%), Positives = 44/63 (69%)
Frame = -3 / +1
Query: 268 ADNEKITSWLKRLEIAEDSAKGIEYLHTGCSPTIIHRDLKSSNILLDKNMRAKVADFGLS 89
A KI W RL IA + +G+ LHT P++IHRDLKSSNILLD++M AKVADFG S
Sbjct: 2296 ASKRKILDWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAKVADFGFS 2475
Query: 88 KPA 80
K A
Sbjct: 2476 KYA 2484
Score = 80.3 bits (169), Expect(3) = 2e-040
Identities = 35/74 (47%), Positives = 50/74 (67%)
Frame = -3 / +1
Query: 475 LADGREIAVKLLTNDSYQGIREFLNEVTLLSRIHHRHLVTFLGYSQQDGKNILVYEFMHN 296
L DG+E+AVK+ ++ S QG +EF NE+ LLS I H +LV LGY + + ILVY FM N
Sbjct: 2086 LDDGQEVAVKVRSSTSTQGTKEFDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSN 2265
Query: 295 GTLKEHLRGADNEK 254
G+L + L G +++
Sbjct: 2266 GSLLDRLYGEASKR 2307
Score = 36.8 bits (74), Expect(3) = 2e-040
Identities = 16/28 (57%), Positives = 17/28 (60%)
Frame = -3 / +1
Query: 100 FGLSKPAVDGSHVSSIVRGTVGYLDPEY 17
F P S+VS VRGT GYLDPEY
Sbjct: 2470 FSKYAPQEGDSYVSLEVRGTAGYLDPEY 2553
>gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 611
Score = 139 bits (299), Expect(2) = 2e-039
Identities = 57/107 (53%), Positives = 76/107 (71%)
Frame = -3 / +1
Query: 409 FLNEVTLLSRIHHRHLVTFLGYSQQDGKNILVYEFMHNGTLKEHLRGADNEKITSWLKRL 230
F+NE+ LSR+HH++LV LG+ + ILVYE+M+NG+L +HL I SW R+
Sbjct: 124 FVNELESLSRLHHKNLVKLLGFYEDKNGRILVYEYMNNGSLNDHLHKFQTSTIMSWNGRI 303
Query: 229 EIAEDSAKGIEYLHTGCSPTIIHRDLKSSNILLDKNMRAKVADFGLS 89
++A D+A+GIEYLH P IIHRD+K+SNILLD AKV+DFGLS
Sbjct: 304 KVALDAARGIEYLHKYAQPPIIHRDIKTSNILLDSKWVAKVSDFGLS 444
Score = 37.3 bits (75), Expect(2) = 2e-039
Identities = 14/26 (53%), Positives = 18/26 (69%)
Frame = -3 / +1
Query: 94 LSKPAVDGSHVSSIVRGTVGYLDPEY 17
L P + SH+S + GTVGY+DPEY
Sbjct: 445 LMGPEDEESHLSLLAAGTVGYMDPEY 522
>gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 596
Score = 51.9 bits (107), Expect(3) = 9e-021
Identities = 19/54 (35%), Positives = 36/54 (66%)
Frame = -3 / +1
Query: 475 LADGREIAVKLLTNDSYQGIREFLNEVTLLSRIHHRHLVTFLGYSQQDGKNILV 314
L DGR +AVK +++ + +F+NEV +L+R+ H++LVT G + + + +L+
Sbjct: 7 LKDGRVVAVKRHYESNFKRVAQFMNEVEILARLRHKNLVTLYGCTSKHSRELLL 168
Score = 48.7 bits (100), Expect(3) = 9e-021
Identities = 18/28 (64%), Positives = 24/28 (85%)
Frame = -3 / +1
Query: 169 IIHRDLKSSNILLDKNMRAKVADFGLSK 86
++HRD+KS+NILLD+ KVADFGLS+
Sbjct: 310 VMHRDVKSNNILLDEKFHVKVADFGLSR 393
Score = 33.6 bits (67), Expect(3) = 9e-021
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = -3 / +1
Query: 76 DGSHVSSIVRGTVGYLDPEY 17
D +HVS+ +GT GY+DPEY
Sbjct: 406 DVTHVSTAPQGTPGYVDPEY 465
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,690,036
Number of Sequences: 7669
Number of extensions: 62032
Number of successful extensions: 1032
Number of sequences better than 1.0e-020: 9
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 170470480
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)