TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCA10g02.yg.2.1
(277 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY851389.1| Medicago sativa leaf sucrose-phosphate synth... 118 3e-043
gb|AY468362.2| Medicago sativa sucrose-phosphate synthase g... 112 3e-039
gb|AF322116.2|AF322116 Medicago sativa sucrose-phosphate sy... 112 4e-039
>gb|AY851389.1| Medicago sativa leaf sucrose-phosphate synthase mRNA, partial cds
Length = 645
Score = 118 bits (252), Expect(2) = 3e-043
Identities = 45/63 (71%), Positives = 55/63 (87%)
Frame = -3 / +3
Query: 191 SNDGEGMGESAGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQVGN 12
+++ + G+S GAYI+RIP GPRDKYL+KE LWP++QEFVDGALAHILNMSK LGEQVG
Sbjct: 186 NDEDDSTGKSRGAYIIRIPFGPRDKYLEKELLWPHIQEFVDGALAHILNMSKVLGEQVGG 365
Query: 11 GRP 3
G+P
Sbjct: 366 GQP 374
Score = 72.5 bits (152), Expect(2) = 1e-022
Identities = 31/47 (65%), Positives = 35/47 (74%)
Frame = +1 / -3
Query: 1 TGLPFPTCSPRALDMLRICARAPSTNSWR*GHSASFLRYLSRGPHGM 141
TG P PTCSP DMLR+ A+APSTNS *G +SF RYLSRGP G+
Sbjct: 376 TGCPPPTCSPNTFDMLRM*AKAPSTNS*M*GQRSSFSRYLSRGPKGI 236
Score = 69.8 bits (146), Expect(2) = 3e-043
Identities = 25/33 (75%), Positives = 30/33 (90%)
Frame = -3 / +3
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGE 177
RVDLFTRQ+SSPD+DWSYGEPTEML +G +D +
Sbjct: 93 RVDLFTRQISSPDIDWSYGEPTEMLSAGPDDND 191
Score = 45.1 bits (92), Expect(2) = 1e-022
Identities = 21/25 (84%), Positives = 22/25 (88%)
Frame = +1 / -3
Query: 202 NISVGSP*LQSTSGDDT*RVKRSTL 276
+ISVGSP*LQS SGDD RVKRSTL
Sbjct: 166 SISVGSP*LQSISGDDIWRVKRSTL 92
>gb|AY468362.2| Medicago sativa sucrose-phosphate synthase gene, promoter region and
complete cds
Length = 8933
Score = 112 bits (240), Expect(2) = 3e-039
Identities = 42/58 (72%), Positives = 51/58 (87%)
Frame = -3 / +1
Query: 182 GEGMGESAGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQVGNG 9
G+ MGES+GAYI+RIP GPR+KY+ KE LWPY+ EFVDGA+ HIL MSKALGEQ+G+G
Sbjct: 3661 GDDMGESSGAYIIRIPFGPRNKYIPKEELWPYIPEFVDGAIGHILQMSKALGEQIGSG 3834
Score = 62.5 bits (130), Expect(2) = 3e-039
Identities = 25/34 (73%), Positives = 27/34 (79%)
Frame = -3 / +1
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGEG 174
RVDL TRQV+SPDVDWSYGEPTEML + D G
Sbjct: 3562 RVDLLTRQVASPDVDWSYGEPTEMLAPRNTDEFG 3663
>gb|AF322116.2|AF322116 Medicago sativa sucrose-phosphate synthase mRNA, complete cds
Length = 3579
Score = 112 bits (240), Expect(2) = 4e-039
Identities = 42/58 (72%), Positives = 51/58 (87%)
Frame = -3 / +2
Query: 182 GEGMGESAGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQVGNG 9
G+ MGES+GAYI+RIP GPR+KY+ KE LWPY+ EFVDGA+ HIL MSKALGEQ+G+G
Sbjct: 875 GDDMGESSGAYIIRIPFGPRNKYIPKEELWPYIPEFVDGAIGHILQMSKALGEQIGSG 1048
Score = 62.5 bits (130), Expect(2) = 4e-039
Identities = 25/34 (73%), Positives = 27/34 (79%)
Frame = -3 / +2
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGEG 174
RVDL TRQV+SPDVDWSYGEPTEML + D G
Sbjct: 776 RVDLLTRQVASPDVDWSYGEPTEMLAPRNTDEFG 877
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 2,674,854
Number of Sequences: 7669
Number of extensions: 31831
Number of successful extensions: 3374
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 39
effective length of database: 949,477
effective search space used: 49372804
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)