TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBL16c05.xg.2.1
(687 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512415.1|CO512415 s13dSG80F0600047_114344 Glandular tr... 136 1e-040
gb|CO511707.1|CO511707 s13dSG01B0800073_103314 Glandular tr... 130 5e-038
gb|CO515771.1|CO515771 s13dSG77F1000091_419691 Glandular tr... 115 2e-032
gb|CO514833.1|CO514833 s13dSG59G1100088_328326 Glandular tr... 58 2e-022
gb|CO513208.1|CO513208 s13dSG24B1200093_129580 Glandular tr... 58 3e-021
>gb|CO512415.1|CO512415 s13dSG80F0600047_114344 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 565
Score = 136 bits (291), Expect(2) = 1e-040
Identities = 53/93 (56%), Positives = 65/93 (69%)
Frame = +2 / +2
Query: 269 LHCVEMGRHSCCYKQKLRKGLWSPEEDEKLMNHITKHGHGCWSSVPKLAGLQRCGKSCRL 448
+H ++MGR CC K L++G W+ EE E L +I +G G W S+PK AGL RCGKSCRL
Sbjct: 149 IH*LDMGRTPCCEKVGLKRGRWTTEEGEILSKYIQANGEGSWRSLPKNAGLLRCGKSCRL 328
Query: 449 RWINYLRPDLKRGAFAQDEEDLIIELHAVLGNR 547
RWINYLR DLKRG + EED I++LH GNR
Sbjct: 329 RWINYLRTDLKRGNISAQEEDTIVKLHTTYGNR 427
Score = 44.6 bits (91), Expect(2) = 1e-040
Identities = 17/23 (73%), Positives = 19/23 (82%)
Frame = +2 / +2
Query: 554 SQIAAQLPGRTDNEIKNLWNSXI 622
S IA+ LPGRTDNEIKN WNS +
Sbjct: 431 SMIASHLPGRTDNEIKNHWNSHL 499
>gb|CO511707.1|CO511707 s13dSG01B0800073_103314 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 570
Score = 130 bits (279), Expect(2) = 5e-038
Identities = 50/88 (56%), Positives = 62/88 (70%)
Frame = +2 / +1
Query: 284 MGRHSCCYKQKLRKGLWSPEEDEKLMNHITKHGHGCWSSVPKLAGLQRCGKSCRLRWINY 463
MGR CC K+ ++KG W+PEED L+ +I +HG G W +VP GL RC KSCRLRW NY
Sbjct: 13 MGRPPCCDKEGVKKGPWTPEEDIILVTYIQEHGPGNWRAVPTKTGLSRCSKSCRLRWTNY 192
Query: 464 LRPDLKRGAFAQDEEDLIIELHAVLGNR 547
LRP +KRG F + EE +II L +LGNR
Sbjct: 193 LRPGIKRGNFTEQEEKMIIHLQDLLGNR 276
Score = 41.4 bits (84), Expect(2) = 5e-038
Identities = 15/27 (55%), Positives = 21/27 (77%)
Frame = +2 / +1
Query: 560 IAAQLPGRTDNEIKNLWNSXIKXXLRQ 640
IA+ LP RTDN+IKN WN+ +K L++
Sbjct: 286 IASYLPQRTDNDIKNYWNTHLKKKLKK 366
>gb|CO515771.1|CO515771 s13dSG77F1000091_419691 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 413
Score = 115 bits (246), Expect(2) = 2e-032
Identities = 45/77 (58%), Positives = 55/77 (71%)
Frame = +2 / +3
Query: 317 LRKGLWSPEEDEKLMNHITKHGHGCWSSVPKLAGLQRCGKSCRLRWINYLRPDLKRGAFA 496
+RKG W+ EED L I K+G G W VPK AGL RC KSCRLRW+NYL P +KR +F+
Sbjct: 93 VRKGAWTYEEDNLLKACINKYGEGKWHLVPKRAGLNRCRKSCRLRWVNYLNPAIKRESFS 272
Query: 497 QDEEDLIIELHAVLGNR 547
DE D+I+ LH +LGNR
Sbjct: 273 HDEIDMILRLHKLLGNR 323
Score = 38.2 bits (77), Expect(2) = 2e-032
Identities = 13/24 (54%), Positives = 20/24 (83%)
Frame = +2 / +3
Query: 554 SQIAAQLPGRTDNEIKNLWNSXIK 625
S IAA+LPGRT N++KN W++ ++
Sbjct: 327 SLIAARLPGRTANDVKNYWHTNLR 398
>gb|CO514833.1|CO514833 s13dSG59G1100088_328326 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 480
Score = 57.9 bits (120), Expect(3) = 2e-022
Identities = 22/41 (53%), Positives = 27/41 (65%)
Frame = +2 / +3
Query: 425 RCGKSCRLRWINYLRPDLKRGAFAQDEEDLIIELHAVLGNR 547
R GKSCRLRW N L P ++ AF +E+D II HA GN+
Sbjct: 153 RSGKSCRLRWCNQLSPQVEHRAFTPEEDDTIIRAHARFGNK 275
Score = 41.8 bits (85), Expect(3) = 2e-022
Identities = 16/29 (55%), Positives = 18/29 (62%)
Frame = +2 / +3
Query: 323 KGLWSPEEDEKLMNHITKHGHGCWSSVPK 409
KG WSPEEDE L + KHG WS + K
Sbjct: 54 KGPWSPEEDEALQKLVEKHGPRNWSIISK 140
Score = 37.3 bits (75), Expect(3) = 2e-022
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = +2 / +3
Query: 560 IAAQLPGRTDNEIKNLWNSXIK 625
IA L GRTDN IKN WNS +K
Sbjct: 285 IARLLSGRTDNAIKNHWNSTLK 350
>gb|CO513208.1|CO513208 s13dSG24B1200093_129580 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 598
Score = 57.9 bits (120), Expect(3) = 3e-021
Identities = 22/41 (53%), Positives = 27/41 (65%)
Frame = +2 / +3
Query: 425 RCGKSCRLRWINYLRPDLKRGAFAQDEEDLIIELHAVLGNR 547
R GKSCRLRW N L P ++ AF +E+D II HA GN+
Sbjct: 255 RSGKSCRLRWCNQLSPQVEHRAFTPEEDDTIIRAHARFGNK 377
Score = 38.2 bits (77), Expect(3) = 3e-021
Identities = 15/29 (51%), Positives = 17/29 (58%)
Frame = +2 / +3
Query: 323 KGLWSPEEDEKLMNHITKHGHGCWSSVPK 409
KG WSPEEDE L + KH WS + K
Sbjct: 156 KGPWSPEEDEALQKLVEKHRPRNWSIISK 242
Score = 37.3 bits (75), Expect(3) = 3e-021
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = +2 / +3
Query: 560 IAAQLPGRTDNEIKNLWNSXIK 625
IA L GRTDN IKN WNS +K
Sbjct: 387 IARLLSGRTDNAIKNHWNSTLK 452
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,564,482
Number of Sequences: 7669
Number of extensions: 75901
Number of successful extensions: 1103
Number of sequences better than 1.0e-020: 6
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 172323420
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)