TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBJ23f06.xg.2.1
(683 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|Y11348.1|MSNANN M.sativa mRNA for annexin-like protein 148 5e-073
emb|X74947.1|MSANN M.sativa mRNA for annexin 144 1e-035
gb|CO511909.1|CO511909 s13dSG05D0900078_103718 Glandular tr... 137 1e-033
gb|CO516777.1|CO516777 s13dSG94C1200098_446882 Glandular tr... 135 5e-033
gb|CO517024.1|CO517024 s13dSG31B1000079_468594 Glandular tr... 130 2e-031
gb|CO514156.1|CO514156 s13dSG75H1000080_156974 Glandular tr... 129 4e-031
gb|CO511849.1|CO511849 s13dSG02F1200103_103598 Glandular tr... 127 1e-030
gb|CO512301.1|CO512301 s13dSG68D0200014_114116 Glandular tr... 125 7e-030
gb|CO513636.1|CO513636 s13dSG17A0100001_140054 Glandular tr... 119 4e-028
gb|CO515484.1|CO515484 s13dSG52B0200025_418027 Glandular tr... 116 3e-027
gb|CO517015.1|CO517015 s13dSG31A1000071_468576 Glandular tr... 115 5e-027
gb|CO516334.1|CO516334 s13dSG99C0300018_445996 Glandular tr... 95 9e-021
>emb|Y11348.1|MSNANN M.sativa mRNA for annexin-like protein
Length = 1533
Score = 148 bits (318), Expect(2) = 5e-073
Identities = 63/111 (56%), Positives = 78/111 (70%)
Frame = +1 / +2
Query: 349 LRAATDIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPE 528
L AAT++ICSRTPSQL +KQ Y+++FG YLEH+I +TSGDHQ +LL Y+ PR+EG E
Sbjct: 416 LEAATEVICSRTPSQLQYLKQLYHSKFGVYLEHEIELNTSGDHQKILLRYLTTPRHEGLE 595
Query: 529 VDPTIXTHDAKDLYKAGEXRLGTDEXXXXXXFTXRXWAHXASXSSAXHHMY 681
V+ I DAK LYKAGE +LGTDE F+ R AH A+ SS H MY
Sbjct: 596 VNREIAQKDAKVLYKAGEKKLGTDEKTFVQIFSERSSAHLAAVSSYYHDMY 748
Score = 140 bits (301), Expect(2) = 5e-073
Identities = 57/99 (57%), Positives = 73/99 (73%)
Frame = +1 / +2
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
MA+L LPP +PR DA+ L +AFKGFGCD+T VINIL HRD+ QR +QQEY+A Y EE
Sbjct: 113 MATLILPPIPPSPRDDAMQLYRAFKGFGCDTTAVINILAHRDATQRAYLQQEYKATYSEE 292
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGD 339
LS R+ SEL G + A+LLW+ DPA RD ++R++L D
Sbjct: 293 LSKRLVSELKGKLETAVLLWLPDPAARDAEIIRKSLVVD 409
Score = 85.8 bits (181), Expect(2) = 1e-032
Identities = 48/109 (44%), Positives = 60/109 (55%)
Frame = -1 / -1
Query: 368 ISVAALRSIVSPLRASLKTVXSRPAGSRIHSNIAFLWFPLSSDEMRWESSS*YIALYSC* 189
I+ A ++S L SR AGS S A FPLSS+ R++SSS Y+ALYSC
Sbjct: 438 ITSVAASRLLSTTSDFLMISASRAAGSGNQSRTAVSNFPLSSETKRFDSSSEYVALYSCW 259
Query: 188 MSPRCTESR*VSMFITVVLSQPNPLKAFWRSMASCRGFAIAGGRVRLAI 42
RC S * + IT V+S PNPLKA MAS G GG++ +AI
Sbjct: 258 R*ARCVASL*ARILITAVVSHPNPLKAR*SCMASSLGEGGIGGKISVAI 112
Score = 68.0 bits (142), Expect(2) = 1e-032
Identities = 43/101 (42%), Positives = 52/101 (51%)
Frame = -1 / -1
Query: 650 AXCAQXRXVXXXXXXXXXXXXXXXPALYKSFASCVXMVGSTSGPS*RGIPT*ASKSXW*S 471
A CA R + PALY++FAS + TS PS RG+ SK *S
Sbjct: 717 AKCALLRSLNIWTNVFSSVPSFFSPALYRTFASF*AISLLTSRPSCRGVVKYLSKIF**S 538
Query: 470 PDVW*PMSCSR*VPNLA**VCFIIXNCDGVLEHIISVAALR 348
P+V+ +SCSR* PN CF + DGV EHI SVAA R
Sbjct: 537 PEVFNSISCSR*TPNFEWYNCFRYCSWDGVREHITSVAASR 415
>emb|X74947.1|MSANN M.sativa mRNA for annexin
Length = 1182
Score = 144 bits (309), Expect = 1e-035
Identities = 65/166 (39%), Positives = 101/166 (60%)
Frame = +1 / +2
Query: 76 NPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEELSHRISSELNG 255
+P +D+ L+ AF+G+G + +I+IL HR++ QR I++ Y + E+L + EL+
Sbjct: 14 SPSEDSEQLRGAFQGWGTNEGLIISILAHRNAAQRKSIRETYTQTHGEDLLKDLDKELSS 193
Query: 256 NHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQLXIMKQTYYARFGT 435
+ +KA+LLW LDPA RD + +A T + +I +R+P +L KQ Y RF
Sbjct: 194 DFEKAVLLWTLDPAERDAFLANQATKMLTSNNSIIVEIASTRSPLELLKAKQAYQVRFKK 373
Query: 436 YLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPTIXTHDAKDLYK 573
LE D+ +HTSGD + LL+ VGI RYEG EV+ T+ +AK L++
Sbjct: 374 SLEEDVAYHTSGDIRKLLVPLVGIHRYEGDEVNMTLAKSEAKLLHE 511
>gb|CO511909.1|CO511909 s13dSG05D0900078_103718 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 565
Score = 137 bits (294), Expect = 1e-033
Identities = 63/175 (36%), Positives = 99/175 (56%)
Frame = +1 / +1
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
M++L++P + D L+KAF G+G + +I+IL HR+ VQR +I++ Y Y E+
Sbjct: 34 MSTLSVPHPLPPVSDDVEQLRKAFSGWGTNEDLIISILGHRNEVQRKVIREAYAKTYEED 213
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQLXI 402
L ++ EL + ++ + LW L+ A RD + EA T + ++ C+R+ QL
Sbjct: 214 LIKALNKELTSDFERLVHLWTLESAERDAFLANEATKRWTSSNQVLVELACTRSSDQLFF 393
Query: 403 MKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPTIXTHDAKDL 567
K+ Y+A LE D+ +HT+GD + LLL V RYEG EV+ TI +AK L
Sbjct: 394 AKKAYHALHKKSLEEDVAYHTTGDFRKLLLPLVSSYRYEGDEVNLTIAKAEAKIL 558
>gb|CO516777.1|CO516777 s13dSG94C1200098_446882 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 554
Score = 135 bits (290), Expect = 5e-033
Identities = 63/177 (35%), Positives = 100/177 (56%)
Frame = +1 / +3
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
M++L++P + D L+KAF G+G + +I+IL HR+ VQR +I++ Y Y E+
Sbjct: 24 MSTLSVPHPLPPVSDDVEQLRKAFSGWGTNEDLIISILGHRNEVQRKVIREAYAKTYEED 203
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQLXI 402
L ++ EL + ++ + LW L+ A RD + EA T + ++ C+R+ QL
Sbjct: 204 LIKALNKELTCDFERLVHLWTLESAERDAFLANEATKRWTSSNQVLVELACTRSSDQLFF 383
Query: 403 MKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPTIXTHDAKDLYK 573
K+ Y+A LE D+ +HT+GD + LLL V RYEG EV TI +AK L++
Sbjct: 384 AKKAYHALHKKSLEEDVAYHTTGDFRKLLLLLVSSYRYEGDEVILTIAKAEAKILHE 554
>gb|CO517024.1|CO517024 s13dSG31B1000079_468594 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 544
Score = 130 bits (279), Expect = 2e-031
Identities = 59/167 (35%), Positives = 94/167 (56%)
Frame = +1 / +3
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
M++L++P + D L+KAF G+G + +I+IL HR+ VQR +I++ Y Y E+
Sbjct: 33 MSTLSVPHPLPPVSDDVEQLRKAFSGWGTNEDLIISILGHRNEVQRKVIREAYAKTYEED 212
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQLXI 402
L ++ EL + ++ + LW L+ A RD + EA T + ++ C+R+ QL
Sbjct: 213 LIKALNKELTSDFERLVHLWTLESAERDAFLANEATKRWTSSNQVLVELACTRSSDQLFF 392
Query: 403 MKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPTI 543
K+ Y+A LE D+ +HT+GD + LLL V RY G EV+ TI
Sbjct: 393 AKKAYHALHKKSLEEDVAYHTTGDFRKLLLPLVSSYRYXGDEVNLTI 533
>gb|CO514156.1|CO514156 s13dSG75H1000080_156974 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 526
Score = 129 bits (276), Expect = 4e-031
Identities = 59/166 (35%), Positives = 93/166 (56%)
Frame = +1 / +1
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
M++L++P + D L+KAF G+G + +I+IL HR+ VQR +I+ Y Y E+
Sbjct: 28 MSTLSVPHPLPPVSDDVEQLRKAFSGWGTNEGLIISILGHRNEVQRKVIRGAYAKTYEED 207
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQLXI 402
L ++ EL + ++ + LW L+ A RD + EA T + ++ C+R+ QL
Sbjct: 208 LIKALNKELTSDFERLVHLWTLESAERDAFLANEATKRWTSSNQVLVELACTRSSDQLFF 387
Query: 403 MKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPT 540
K+ Y+A LE D+ +HT+GD + LLL V RYEG EV+ T
Sbjct: 388 AKKAYHALHKKSLEEDVAYHTTGDFRKLLLPLVSSYRYEGDEVNLT 525
>gb|CO511849.1|CO511849 s13dSG02F1200103_103598 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 558
Score = 127 bits (273), Expect = 1e-030
Identities = 57/162 (35%), Positives = 91/162 (56%)
Frame = +1 / +3
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
M++L++P + D L+KAF G+G + +I+IL HR+ VQR +I++ Y Y E+
Sbjct: 72 MSTLSVPHPLPPVSDDVEQLRKAFSGWGTNEGLIISILGHRNEVQRKVIREAYAKTYEED 251
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQLXI 402
L ++ EL + ++ + LW L+ A RD + EA T + ++ C+R+ QL
Sbjct: 252 LIKALNKELTSDFERLVHLWTLESAERDAFLANEATKRWTSSNQVLVELACTRSSDQLFF 431
Query: 403 MKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPE 528
K+ Y+A LE D+ +HT+GD + LLL V RYEG E
Sbjct: 432 AKKAYHALHKKSLEEDVAYHTTGDFRKLLLPLVSSYRYEGDE 557
>gb|CO512301.1|CO512301 s13dSG68D0200014_114116 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 547
Score = 125 bits (267), Expect = 7e-030
Identities = 57/161 (35%), Positives = 90/161 (55%)
Frame = +1 / +3
Query: 61 PPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEELSHRIS 240
P + + D L+KAF G+G + +I+IL HR+ VQR +I++ Y Y E+L ++
Sbjct: 51 PSSSSPVSDDVEQLRKAFSGWGTNEGLIISILGHRNEVQRKVIREAYAKTYEEDLIKALN 230
Query: 241 SELNGNHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQLXIMKQTYY 420
EL + ++ + LW L+ A RD + EA T + ++ C+R+ QL K+ Y+
Sbjct: 231 KELTSDFERLVHLWTLESAERDAFLANEATKRWTSSNQVLVELACTRSSDQLFFAKKAYH 410
Query: 421 ARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPTI 543
A LE D+ ++T+GD + LLL V RYEG EV+ TI
Sbjct: 411 ALHKKSLEEDVAYYTTGDFRKLLLPLVSSYRYEGDEVNLTI 533
>gb|CO513636.1|CO513636 s13dSG17A0100001_140054 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 496
Score = 119 bits (254), Expect = 4e-028
Identities = 53/153 (34%), Positives = 87/153 (56%)
Frame = +1 / +1
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
M++L++P + D L+KAF G+G + +I+IL HR+ VQR +I++ Y Y E+
Sbjct: 28 MSTLSVPHPLPPVSDDVEQLRKAFSGWGTNEGLIISILGHRNEVQRKVIREAYAKTYEED 207
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQLXI 402
L ++ EL + ++ + LWIL+ A RD + EA T + ++ C+R+ QL
Sbjct: 208 LIKALNKELASDFERLVHLWILESAERDAFLANEATKRWTSSNQVLVELACTRSSDQLFF 387
Query: 403 MKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYV 501
K+ Y+A LE D+ +HT+GD + LLL V
Sbjct: 388 AKKAYHALHKKSLEEDVAYHTTGDFRKLLLPLV 486
>gb|CO515484.1|CO515484 s13dSG52B0200025_418027 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 501
Score = 116 bits (248), Expect = 3e-027
Identities = 51/150 (34%), Positives = 85/150 (56%)
Frame = +1 / +2
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
M++L++P + D L+KAF G+G + +I+IL HR+ VQR +I++ Y Y E+
Sbjct: 44 MSTLSVPHPLPPVSDDVEQLRKAFSGWGTNEGLIISILGHRNEVQRKVIREAYAKTYDED 223
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQLXI 402
L ++ EL + ++ + LW L+ A RD + EA T + ++ C+R+ QL
Sbjct: 224 LIKALNKELTSDFERLVHLWTLESAERDAFLANEATKRWTSSNQVLVELACTRSSDQLFF 403
Query: 403 MKQTYYARFGTYLEHDIGHHTSGDHQXLLL 492
K+ Y+A LE D+ +HT+GD + LLL
Sbjct: 404 AKKAYHALHKKSLEEDVAYHTTGDFRKLLL 493
>gb|CO517015.1|CO517015 s13dSG31A1000071_468576 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 479
Score = 115 bits (246), Expect = 5e-027
Identities = 50/149 (33%), Positives = 84/149 (56%)
Frame = +1 / +3
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
M++L++P + D L+KAF G+G + +I+IL HR+ VQR +I++ Y Y E+
Sbjct: 33 MSTLSVPHPLPPVSDDVEQLRKAFSGWGTNEDLIISILGHRNEVQRKVIREAYAKTYEED 212
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQLXI 402
L ++ EL + ++ + LW L+ A RD + EA T + ++ C+R+ QL
Sbjct: 213 LIKALNKELTSDFERLVHLWTLESAERDAFLANEATKRWTSSNQVLVELACTRSSDQLFF 392
Query: 403 MKQTYYARFGTYLEHDIGHHTSGDHQXLL 489
K+ Y+A LE D+ +HT+GD + LL
Sbjct: 393 AKKAYHALHKKSLEEDVAYHTTGDFRKLL 479
>gb|CO516334.1|CO516334 s13dSG99C0300018_445996 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 406
Score = 95.0 bits (201), Expect = 9e-021
Identities = 41/127 (32%), Positives = 71/127 (55%)
Frame = +1 / +2
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
M++L++P + D L+KAF G+G + +I+IL HR+ VQR +I++ Y Y E+
Sbjct: 8 MSTLSVPHPLPPVSDDVEQLRKAFSGWGTNEDLIISILGHRNEVQRKVIREAYAKTYEED 187
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQLXI 402
L ++ EL + ++ + LW L+ A RD + EA T + ++ C+R+ QL
Sbjct: 188 LIKALNKELTSDFERLVHLWTLESAERDAFLANEATKRWTSSNQVLVELACTRSSDQLFF 367
Query: 403 MKQTYYA 423
K+ Y+A
Sbjct: 368 AKKAYHA 388
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,322,021
Number of Sequences: 7669
Number of extensions: 66861
Number of successful extensions: 792
Number of sequences better than 1.0e-020: 13
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 171396950
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)