TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF30a10.yg.2.1
(1130 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|AX776636.1| Sequence 5 from Patent WO02102841 102 5e-054
emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulatio... 102 5e-054
gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated... 108 3e-053
emb|AX776635.1| Sequence 4 from Patent WO02102841 101 3e-051
emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulatio... 101 3e-051
gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular tr... 71 5e-038
gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular tr... 95 6e-035
gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular tr... 79 6e-033
gb|CO512977.1|CO512977 s13dSG09B0200013_121880 Glandular tr... 73 1e-027
>emb|AX776636.1| Sequence 5 from Patent WO02102841
Length = 3339
Score = 102 bits (218), Expect(3) = 5e-054
Identities = 43/83 (51%), Positives = 57/83 (68%)
Frame = +3 / +3
Query: 300 LLGEGGYGSVYKGKLVDGSVVAIKQLSETSRQGKKEFVAEIETISRVQHRNLVKLFGFCL 479
L+GEGG+GSVY+G L DG VA+K S TS QG EF E+ +S +QH NLV L G+C
Sbjct: 2046 LIGEGGFGSVYRGTLDDGQEVAVKVRSSTSTQGTXEFDNELNLLSAIQHENLVPLLGYCN 2225
Query: 480 EGNKPLLVYEYMESGSLDKALFG 548
E ++ +LVY +M +GSL L+G
Sbjct: 2226 EYDQQILVYPFMSNGSLLDRLYG 2294
Score = 77.1 bits (162), Expect(3) = 5e-054
Identities = 29/55 (52%), Positives = 40/55 (72%)
Frame = +3 / +3
Query: 564 LNWSTRYKICLGIARGLAYLHEESSIRIVHRDIKASNVLLDATLNPKISDFGLAK 728
L+W TR I LG ARGLAYLH ++HRD+K+SN+LLD ++ K++DFG +K
Sbjct: 2316 LDWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAKVADFGFSK 2480
Score = 65.2 bits (136), Expect(3) = 5e-054
Identities = 30/77 (38%), Positives = 43/77 (55%)
Frame = +3 / +3
Query: 747 THVSTKVAGTFGYLSPEYAMRGHMTEKVDIFAFGVVMLEIIAGRPNYDGKLDXXMAYLLX 926
++VS +V GT GYL PEY ++EK D+F+FGVV+LEI++GR + K L+
Sbjct: 2502 SYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWSLVE 2681
Query: 927 WVWQLYEEDHPLDIADP 977
W +I DP
Sbjct: 2682 WAKPYIRASKVDEIVDP 2732
>emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulation receptor kinase (nork gene), wild
type allele DMI2
Length = 3339
Score = 102 bits (218), Expect(3) = 5e-054
Identities = 43/83 (51%), Positives = 57/83 (68%)
Frame = +3 / +3
Query: 300 LLGEGGYGSVYKGKLVDGSVVAIKQLSETSRQGKKEFVAEIETISRVQHRNLVKLFGFCL 479
L+GEGG+GSVY+G L DG VA+K S TS QG EF E+ +S +QH NLV L G+C
Sbjct: 2046 LIGEGGFGSVYRGTLDDGQEVAVKVRSSTSTQGTXEFDNELNLLSAIQHENLVPLLGYCN 2225
Query: 480 EGNKPLLVYEYMESGSLDKALFG 548
E ++ +LVY +M +GSL L+G
Sbjct: 2226 EYDQQILVYPFMSNGSLLDRLYG 2294
Score = 77.1 bits (162), Expect(3) = 5e-054
Identities = 29/55 (52%), Positives = 40/55 (72%)
Frame = +3 / +3
Query: 564 LNWSTRYKICLGIARGLAYLHEESSIRIVHRDIKASNVLLDATLNPKISDFGLAK 728
L+W TR I LG ARGLAYLH ++HRD+K+SN+LLD ++ K++DFG +K
Sbjct: 2316 LDWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAKVADFGFSK 2480
Score = 65.2 bits (136), Expect(3) = 5e-054
Identities = 30/77 (38%), Positives = 43/77 (55%)
Frame = +3 / +3
Query: 747 THVSTKVAGTFGYLSPEYAMRGHMTEKVDIFAFGVVMLEIIAGRPNYDGKLDXXMAYLLX 926
++VS +V GT GYL PEY ++EK D+F+FGVV+LEI++GR + K L+
Sbjct: 2502 SYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWSLVE 2681
Query: 927 WVWQLYEEDHPLDIADP 977
W +I DP
Sbjct: 2682 WAKPYIRASKVDEIVDP 2732
>gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated receptor-like kinase
mRNA, complete cds
Length = 1798
Score = 108 bits (230), Expect(3) = 3e-053
Identities = 46/112 (41%), Positives = 71/112 (63%)
Frame = +3 / +1
Query: 405 EFVAEIETISRVQHRNLVKLFGFCLEGNKPLLVYEYMESGSLDKALFGNGRLNLNWSTRY 584
EF EI T+S+++H NLV+L+G+ G++ L++ EY+ +G+L + L G L R
Sbjct: 685 EFKNEIYTLSKIEHLNLVRLYGYLEHGDEKLIIVEYVGNGNLTEHLDGIRGDGLEIGERL 864
Query: 585 KICLGIARGLAYLHEESSIRIVHRDIKASNVLLDATLNPKISDFGLAKLYDD 740
I + IA + YLH + I+HRDIKASN+L+ L K++DFG A+L +D
Sbjct: 865 DIAIDIAHAITYLHMYTDNPIIHRDIKASNILISENLRAKVADFGFARLSED 1020
Score = 71.6 bits (150), Expect(3) = 3e-053
Identities = 28/83 (33%), Positives = 47/83 (56%)
Frame = +3 / +1
Query: 735 DDKKTHVSTKVAGTFGYLSPEYAMRGHMTEKVDIFAFGVVMLEIIAGRPNYDGKLDXXMA 914
D TH+ST+V GT GY+ PEY +TEK D+++FGV+++E++ GR + K
Sbjct: 1018 DPGATHISTQVKGTAGYMDPEYLRTYQLTEKSDVYSFGVLLVEMMTGRHPVEPKKKIDER 1197
Query: 915 YLLXWVWQLYEEDHPLDIADPKL 983
+ W ++ + + DP+L
Sbjct: 1198 VTIRWAMKMLKNGDAVFAMDPRL 1266
Score = 62.5 bits (130), Expect(3) = 3e-053
Identities = 23/49 (46%), Positives = 37/49 (75%)
Frame = +3 / +1
Query: 249 YTYGELRTATENFSSANLLGEGGYGSVYKGKLVDGSVVAIKQLSETSRQ 395
+T+ E+ +T FSS N +GEGG+G+VY+GKL DG++VA+K+ + + Q
Sbjct: 526 FTFEEIYKSTAKFSSDNQIGEGGFGTVYRGKLNDGTIVAVKRAKKEALQ 672
>emb|AX776635.1| Sequence 4 from Patent WO02102841
Length = 3378
Score = 101 bits (215), Expect(3) = 3e-051
Identities = 43/83 (51%), Positives = 57/83 (68%)
Frame = +3 / +1
Query: 300 LLGEGGYGSVYKGKLVDGSVVAIKQLSETSRQGKKEFVAEIETISRVQHRNLVKLFGFCL 479
L+ EGG+GSVY+G L DG VA+K S TS QG KEF E+ +S +QH NLV L G+C
Sbjct: 2065 LICEGGFGSVYRGTLDDGQEVAVKVRSSTSTQGTKEFDNELNLLSAIQHENLVPLLGYCN 2244
Query: 480 EGNKPLLVYEYMESGSLDKALFG 548
E ++ +LVY +M +GSL L+G
Sbjct: 2245 EYDQQILVYPFMSNGSLLDRLYG 2313
Score = 69.3 bits (145), Expect(3) = 3e-051
Identities = 27/55 (49%), Positives = 38/55 (69%)
Frame = +3 / +1
Query: 564 LNWSTRYKICLGIARGLAYLHEESSIRIVHRDIKASNVLLDATLNPKISDFGLAK 728
L+W TR I LG RGLA LH ++HRD+K+SN+LLD ++ K++DFG +K
Sbjct: 2335 LDWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAKVADFGFSK 2499
Score = 65.2 bits (136), Expect(3) = 3e-051
Identities = 30/77 (38%), Positives = 43/77 (55%)
Frame = +3 / +1
Query: 747 THVSTKVAGTFGYLSPEYAMRGHMTEKVDIFAFGVVMLEIIAGRPNYDGKLDXXMAYLLX 926
++VS +V GT GYL PEY ++EK D+F+FGVV+LEI++GR + K L+
Sbjct: 2521 SYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWSLVE 2700
Query: 927 WVWQLYEEDHPLDIADP 977
W +I DP
Sbjct: 2701 WAKPYIRASKVDEIVDP 2751
>emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulation receptor kinase (nork gene),
mutant allele nn1
Length = 3337
Score = 101 bits (215), Expect(3) = 3e-051
Identities = 43/83 (51%), Positives = 57/83 (68%)
Frame = +3 / +1
Query: 300 LLGEGGYGSVYKGKLVDGSVVAIKQLSETSRQGKKEFVAEIETISRVQHRNLVKLFGFCL 479
L+ EGG+GSVY+G L DG VA+K S TS QG KEF E+ +S +QH NLV L G+C
Sbjct: 2044 LICEGGFGSVYRGTLDDGQEVAVKVRSSTSTQGTKEFDNELNLLSAIQHENLVPLLGYCN 2223
Query: 480 EGNKPLLVYEYMESGSLDKALFG 548
E ++ +LVY +M +GSL L+G
Sbjct: 2224 EYDQQILVYPFMSNGSLLDRLYG 2292
Score = 69.3 bits (145), Expect(3) = 3e-051
Identities = 27/55 (49%), Positives = 38/55 (69%)
Frame = +3 / +1
Query: 564 LNWSTRYKICLGIARGLAYLHEESSIRIVHRDIKASNVLLDATLNPKISDFGLAK 728
L+W TR I LG RGLA LH ++HRD+K+SN+LLD ++ K++DFG +K
Sbjct: 2314 LDWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAKVADFGFSK 2478
Score = 65.2 bits (136), Expect(3) = 3e-051
Identities = 30/77 (38%), Positives = 43/77 (55%)
Frame = +3 / +1
Query: 747 THVSTKVAGTFGYLSPEYAMRGHMTEKVDIFAFGVVMLEIIAGRPNYDGKLDXXMAYLLX 926
++VS +V GT GYL PEY ++EK D+F+FGVV+LEI++GR + K L+
Sbjct: 2500 SYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWSLVE 2679
Query: 927 WVWQLYEEDHPLDIADP 977
W +I DP
Sbjct: 2680 WAKPYIRASKVDEIVDP 2730
>gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 611
Score = 70.7 bits (148), Expect(3) = 5e-038
Identities = 27/54 (50%), Positives = 39/54 (72%)
Frame = +3 / +1
Query: 564 LNWSTRYKICLGIARGLAYLHEESSIRIVHRDIKASNVLLDATLNPKISDFGLA 725
++W+ R K+ L ARG+ YLH+ + I+HRDIK SN+LLD+ K+SDFGL+
Sbjct: 283 MSWNGRIKVALDAARGIEYLHKYAQPPIIHRDIKTSNILLDSKWVAKVSDFGLS 444
Score = 60.6 bits (126), Expect(3) = 5e-038
Identities = 25/45 (55%), Positives = 34/45 (75%)
Frame = +3 / +1
Query: 408 FVAEIETISRVQHRNLVKLFGFCLEGNKPLLVYEYMESGSLDKAL 542
FV E+E++SR+ H+NLVKL GF + N +LVYEYM +GSL+ L
Sbjct: 124 FVNELESLSRLHHKNLVKLLGFYEDKNGRILVYEYMNNGSLNDHL 258
Score = 59.7 bits (124), Expect(3) = 5e-038
Identities = 20/47 (42%), Positives = 35/47 (74%)
Frame = +3 / +1
Query: 735 DDKKTHVSTKVAGTFGYLSPEYAMRGHMTEKVDIFAFGVVMLEIIAG 875
+D+++H+S AGT GY+ PEY ++T K D+++FGV +LE+++G
Sbjct: 457 EDEESHLSLLAAGTVGYMDPEYYRLQYLTSKSDVYSFGVXLLELLSG 597
>gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 596
Score = 95.0 bits (201), Expect(4) = 6e-035
Identities = 35/76 (46%), Positives = 57/76 (75%)
Frame = +3 / +1
Query: 645 IVHRDIKASNVLLDATLNPKISDFGLAKLYDDKKTHVSTKVAGTFGYLSPEYAMRGHMTE 824
++HRD+K++N+LLD + K++DFGL++L+ + THVST GT GY+ PEY +T+
Sbjct: 310 VMHRDVKSNNILLDEKFHVKVADFGLSRLFPNDVTHVSTAPQGTPGYVDPEYYQCYQLTD 489
Query: 825 KVDIFAFGVVMLEIIA 872
K D ++FGVV++E+I+
Sbjct: 490 KSDAYSFGVVLVELIS 537
Score = 50.6 bits (104), Expect(4) = 6e-035
Identities = 21/56 (37%), Positives = 39/56 (69%)
Frame = +3 / +1
Query: 336 GKLVDGSVVAIKQLSETSRQGKKEFVAEIETISRVQHRNLVKLFGFCLEGNKPLLV 503
G L DG VVA+K+ E++ + +F+ E+E ++R++H+NLV L+G + ++ LL+
Sbjct: 1 GDLKDGRVVAVKRHYESNFKRVAQFMNEVEILARLRHKNLVTLYGCTSKHSRELLL 168
Score = 28.6 bits (56), Expect(4) = 6e-035
Identities = 12/21 (57%), Positives = 12/21 (57%)
Frame = +3 / +1
Query: 564 LNWSTRYKICLGIARGLAYLH 626
L WS R I L A LAYLH
Sbjct: 238 LPWSVRLDIALETAEALAYLH 300
Score = 24.0 bits (46), Expect(4) = 6e-035
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +3 / +1
Query: 495 LLVYEYMESGSLDKALFGN 551
LLVYEY+ +G++ L G+
Sbjct: 163 LLVYEYISNGTVADHLHGD 219
>gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 595
Score = 79.0 bits (166), Expect(2) = 6e-033
Identities = 31/69 (44%), Positives = 44/69 (63%)
Frame = +3 / +1
Query: 744 KTHVSTKVAGTFGYLSPEYAMRGHMTEKVDIFAFGVVMLEIIAGRPNYDGKLDXXMAYLL 923
KTHVST+V GT+GY +P+YAM G +T K DI++ GV +LE+I GR D + L+
Sbjct: 235 KTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSIGVALLELITGRKAIDHRRPAKEQNLV 414
Query: 924 XWVWQLYEE 950
W L+ +
Sbjct: 415 AWARPLFRD 441
Score = 77.1 bits (162), Expect(2) = 6e-033
Identities = 30/60 (50%), Positives = 43/60 (71%)
Frame = +3 / +1
Query: 552 GRLNLNWSTRYKICLGIARGLAYLHEESSIRIVHRDIKASNVLLDATLNPKISDFGLAKL 731
G+ L+W+TR KI G ARGL YLH++ +++RD+K SN+LL + K+SDFGLAK+
Sbjct: 40 GQKPLDWNTRMKIAAGAARGLEYLHDKMKPPVIYRDLKCSNILLGEDYHSKLSDFGLAKV 219
>gb|CO512977.1|CO512977 s13dSG09B0200013_121880 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 582
Score = 73.0 bits (153), Expect(3) = 1e-027
Identities = 31/56 (55%), Positives = 37/56 (66%)
Frame = +3 / +1
Query: 564 LNWSTRYKICLGIARGLAYLHEESSIRIVHRDIKASNVLLDATLNPKISDFGLAKL 731
L WS R KI G A+GL YLHE S + VH D+K SN+LL + P ISDFGL +L
Sbjct: 175 LTWSDRLKIMKGTAKGLVYLHEFSPKKYVHGDLKPSNILLGHDMTPYISDFGLGRL 342
Score = 46.9 bits (96), Expect(3) = 1e-027
Identities = 18/53 (33%), Positives = 32/53 (60%)
Frame = +3 / +1
Query: 402 KEFVAEIETISRVQHRNLVKLFGFCLEGNKPLLVYEYMESGSLDKALFGNGRL 560
+EF E+E I +++H N+ L + ++ LL+Y+Y+ +GSL A+ G L
Sbjct: 1 REFQTEVEAIGKLRHPNIATLRAYYWSVDEKLLIYDYIPNGSLASAIHGKAGL 159
Score = 35.9 bits (72), Expect(3) = 1e-027
Identities = 13/33 (39%), Positives = 23/33 (69%)
Frame = +3 / +1
Query: 780 GYLSPEYAMRGHMTEKVDIFAFGVVMLEIIAGR 878
GY +PE ++K D++++GV++LE+I GR
Sbjct: 457 GYQAPEALKVVKPSQKWDVYSYGVILLEMITGR 555
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,722,602
Number of Sequences: 7669
Number of extensions: 125162
Number of successful extensions: 1330
Number of sequences better than 1.0e-020: 12
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 305960733
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)