TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF18e02.yg.3.1
(315 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512512.1|CO512512 s13dSG100H110009_114538 Glandular tr... 28 0.43
gb|CO513016.1|CO513016 s13dSG09F0400031_121958 Glandular tr... 28 0.43
gb|CO514321.1|CO514321 s13dSG84B0100009_157304 Glandular tr... 28 0.43
gb|CO514652.1|CO514652 s13dSG46D0700062_327964 Glandular tr... 28 0.43
gb|CO514722.1|CO514722 s13dSG55C1200098_328104 Glandular tr... 28 0.43
gb|CO514756.1|CO514756 s13dSG55G0900072_328172 Glandular tr... 28 0.43
gb|CO514967.1|CO514967 s13dSG63F0500047_397439 Glandular tr... 28 0.43
gb|CO516716.1|CO516716 s13dSG74E0200007_446760 Glandular tr... 28 0.44
gb|CO514861.1|CO514861 s13dSG56C0300022_328382 Glandular tr... 25 0.77
gb|CO515478.1|CO515478 s13dSG52A0700053_418015 Glandular tr... 22 0.92
gb|CO511922.1|CO511922 s13dSG05F0200027_103744 Glandular tr... 25 1.4
gb|CO514818.1|CO514818 s13dSG59F0200027_328296 Glandular tr... 26 1.5
gb|CO514980.1|CO514980 s13dSG63H0100016_397465 Glandular tr... 26 1.5
gb|CO512953.1|CO512953 s13dSG86G0800056_121832 Glandular tr... 26 2.1
gb|CO512102.1|CO512102 s13dSG18H0500044_108524 Glandular tr... 25 4.0
gb|CO516870.1|CO516870 s13dSG98H0400041_447068 Glandular tr... 25 4.0
emb|CQ760957.1| Sequence 2 from Patent WO2004002216 25 4.0
emb|CQ760963.1| Sequence 8 from Patent WO2004002216 25 4.0
gb|DQ122772.1| Medicago sativa clone ME10 putative translat... 25 4.0
gb|CO516274.1|CO516274 s13dSG69E0100003_445876 Glandular tr... 21 5.0
emb|AJ248322.1|MSA248322 Medicago sativa subsp. X varia mRN... 24 5.4
gb|CO513550.1|CO513550 s13dSG12G0900068_139882 Glandular tr... 24 5.4
gb|CO516580.1|CO516580 s13dSG57H0200016_446488 Glandular tr... 24 5.4
gb|CO517028.1|CO517028 s13dSG31C0900068_468602 Glandular tr... 21 6.7
gb|CO512134.1|CO512134 s13dSG34C0700050_108588 Glandular tr... 21 7.2
gb|AW698629.1|AW698629 g676 glandular-haired subtracted cDN... 24 7.5
gb|CO513126.1|CO513126 s13dSG23B0900073_129416 Glandular tr... 24 7.5
gb|CO515118.1|CO515118 s13dSG40D1000090_399101 Glandular tr... 24 7.5
gb|DQ122767.1| Medicago sativa clone QC5 serine carboxypept... 24 7.5
gb|CO511814.1|CO511814 s13dSG02C1200098_103528 Glandular tr... 21 7.6
gb|CO513242.1|CO513242 s13dSG24F0600047_129648 Glandular tr... 21 7.7
>gb|CO512512.1|CO512512 s13dSG100H110009_114538 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 595
Score = 28.1 bits (55), Expect = 0.43
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +1 / +1
Query: 13 KLLLLVDHLSNHHSNPIFHIFPLIHMQSSW 102
+++LL DH +HH P H+ L+ W
Sbjct: 136 QVMLLRDHYHHHHVFPHHHLLILLQHHHLW 225
>gb|CO513016.1|CO513016 s13dSG09F0400031_121958 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 525
Score = 28.1 bits (55), Expect = 0.43
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +1 / +2
Query: 13 KLLLLVDHLSNHHSNPIFHIFPLIHMQSSW 102
+++LL DH +HH P H+ L+ W
Sbjct: 74 QVMLLRDHYHHHHVFPHHHLLILLQHHHLW 163
>gb|CO514321.1|CO514321 s13dSG84B0100009_157304 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 530
Score = 28.1 bits (55), Expect = 0.43
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +1 / +1
Query: 13 KLLLLVDHLSNHHSNPIFHIFPLIHMQSSW 102
+++LL DH +HH P H+ L+ W
Sbjct: 136 QVMLLRDHYHHHHVFPHHHLLILLQHHHLW 225
>gb|CO514652.1|CO514652 s13dSG46D0700062_327964 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 589
Score = 28.1 bits (55), Expect = 0.43
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +1 / +1
Query: 13 KLLLLVDHLSNHHSNPIFHIFPLIHMQSSW 102
+++LL DH +HH P H+ L+ W
Sbjct: 157 QVMLLRDHYHHHHVFPHHHLLILLQHHHLW 246
>gb|CO514722.1|CO514722 s13dSG55C1200098_328104 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 530
Score = 28.1 bits (55), Expect = 0.43
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +1 / +3
Query: 13 KLLLLVDHLSNHHSNPIFHIFPLIHMQSSW 102
+++LL DH +HH P H+ L+ W
Sbjct: 132 QVMLLRDHYHHHHVFPHHHLLILLQHHHLW 221
>gb|CO514756.1|CO514756 s13dSG55G0900072_328172 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 510
Score = 28.1 bits (55), Expect = 0.43
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +1 / +1
Query: 13 KLLLLVDHLSNHHSNPIFHIFPLIHMQSSW 102
+++LL DH +HH P H+ L+ W
Sbjct: 157 QVMLLRDHYHHHHVFPHHHLLILLQHHHLW 246
>gb|CO514967.1|CO514967 s13dSG63F0500047_397439 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 421
Score = 28.1 bits (55), Expect = 0.43
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +1 / +1
Query: 13 KLLLLVDHLSNHHSNPIFHIFPLIHMQSSW 102
+++LL DH +HH P H+ L+ W
Sbjct: 136 QVMLLRDHYHHHHVFPHHHLLILLQHHHLW 225
>gb|CO516716.1|CO516716 s13dSG74E0200007_446760 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 321
Score = 27.6 bits (54), Expect(2) = 0.44
Identities = 10/31 (32%), Positives = 16/31 (51%)
Frame = +1 / +1
Query: 13 KLLLLVDHLSNHHSNPIFHIFPLIHMQSSWL 105
+++ L H +HH P H+F L+ WL
Sbjct: 139 QVMFLYHHHHHHHVFPHHHLFILLQHHHLWL 231
Score = 15.7 bits (28), Expect(2) = 0.44
Identities = 4/6 (66%), Positives = 5/6 (83%)
Frame = +1 / +1
Query: 220 HLIPDH 237
HL+P H
Sbjct: 244 HLLPHH 261
>gb|CO514861.1|CO514861 s13dSG56C0300022_328382 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 589
Score = 24.9 bits (48), Expect(2) = 0.77
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = -2 / +1
Query: 218 SFLFLSIFLNGPHSNRNNL 162
+FLFLS + N P S+ N L
Sbjct: 31 AFLFLSHYTNSPRSDPNPL 87
Score = 18.5 bits (34), Expect(2) = 0.77
Identities = 4/5 (80%), Positives = 5/5 (100%)
Frame = -2 / +2
Query: 146 FIWCY 132
+IWCY
Sbjct: 341 YIWCY 355
>gb|CO515478.1|CO515478 s13dSG52A0700053_418015 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 521
Score = 21.7 bits (41), Expect(2) = 0.92
Identities = 6/13 (46%), Positives = 11/13 (84%)
Frame = +2 / -3
Query: 62 YFIFFH*FICKAR 100
+FIF++ +CK+R
Sbjct: 183 FFIFYNIVVCKSR 145
Score = 21.2 bits (40), Expect(2) = 0.92
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = +1 / -1
Query: 7 PSKLLLLVDHLSNHHSNPI 63
PSK L+ + +HH NP+
Sbjct: 374 PSKQLIPPNSNIHHHLNPL 318
>gb|CO511922.1|CO511922 s13dSG05F0200027_103744 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 604
Score = 24.9 bits (48), Expect(2) = 1.4
Identities = 9/24 (37%), Positives = 12/24 (50%)
Frame = +1 / +1
Query: 34 HLSNHHSNPIFHIFPLIHMQSSWL 105
H +HH P H+F L+ WL
Sbjct: 160 HHHHHHVFPHHHLFILLQHHHLWL 231
Score = 17.6 bits (32), Expect(2) = 1.4
Identities = 5/7 (71%), Positives = 6/7 (85%)
Frame = +1 / +1
Query: 217 LHLIPDH 237
LHL+P H
Sbjct: 364 LHLLPHH 384
>gb|CO514818.1|CO514818 s13dSG59F0200027_328296 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 503
Score = 26.3 bits (51), Expect = 1.5
Identities = 9/31 (29%), Positives = 16/31 (51%)
Frame = +1 / +3
Query: 13 KLLLLVDHLSNHHSNPIFHIFPLIHMQSSWL 105
+++ L H +HH P H+F L+ W+
Sbjct: 138 QVMFLYHHHHHHHVFPHHHLFILLQHHHLWV 230
>gb|CO514980.1|CO514980 s13dSG63H0100016_397465 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 195
Score = 26.3 bits (51), Expect = 1.5
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +1 / -3
Query: 31 DHLSNHHSNPIFHIFPLIH 87
DH S H +P H+FPL++
Sbjct: 85 DH*SEHPVHPRNHLFPLLY 29
>gb|CO512953.1|CO512953 s13dSG86G0800056_121832 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 585
Score = 25.8 bits (50), Expect = 2.1
Identities = 6/10 (60%), Positives = 8/10 (80%)
Frame = +2 / -2
Query: 11 ANCCSWWIIS 40
+ CCSWW +S
Sbjct: 431 SRCCSWWCLS 402
>gb|CO512102.1|CO512102 s13dSG18H0500044_108524 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 542
Score = 24.9 bits (48), Expect = 4.0
Identities = 8/16 (50%), Positives = 13/16 (81%)
Frame = +1 / -2
Query: 16 LLLLVDHLSNHHSNPI 63
L+ +V HLSNH ++P+
Sbjct: 199 LVHVVPHLSNHQASPV 152
>gb|CO516870.1|CO516870 s13dSG98H0400041_447068 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 476
Score = 24.9 bits (48), Expect = 4.0
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = +1 / -1
Query: 55 NPIFHIFPLIHMQS 96
NPIF +PLIH+ S
Sbjct: 206 NPIFLKYPLIHLPS 165
>emb|CQ760957.1| Sequence 2 from Patent WO2004002216
Length = 678
Score = 24.9 bits (48), Expect = 4.0
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +3 / +1
Query: 180 VRTIQKDAEEKKASFNPRP 236
VRTIQ D E K P P
Sbjct: 325 VRTIQNDINELKKQLKPAP 381
>emb|CQ760963.1| Sequence 8 from Patent WO2004002216
Length = 1482
Score = 24.9 bits (48), Expect = 4.0
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +3 / +3
Query: 180 VRTIQKDAEEKKASFNPRP 236
VRTIQ D E K P P
Sbjct: 885 VRTIQNDINELKKQLKPAP 941
>gb|DQ122772.1| Medicago sativa clone ME10 putative translation initiation factor
mRNA, partial cds
Length = 419
Score = 24.9 bits (48), Expect = 4.0
Identities = 10/33 (30%), Positives = 16/33 (48%)
Frame = +1 / -1
Query: 7 PSKLLLLVDHLSNHHSNPIFHIFPLIHMQSSWL 105
P LL + L H +P +H+FP +W+
Sbjct: 401 PKHRLLSILGLFQHGIHP*WHLFPFEACHRAWM 303
>gb|CO516274.1|CO516274 s13dSG69E0100003_445876 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 312
Score = 20.8 bits (39), Expect(3) = 5.0
Identities = 7/20 (35%), Positives = 10/20 (50%)
Frame = +1 / +2
Query: 43 NHHSNPIFHIFPLIHMQSSW 102
+HH P H+F L+ W
Sbjct: 158 HHHVFPHHHLFILLRHHYLW 217
Score = 16.2 bits (29), Expect(3) = 5.0
Identities = 7/12 (58%), Positives = 8/12 (66%)
Frame = +1 / +2
Query: 16 LLLLVDHLSNHH 51
LLL V L +HH
Sbjct: 128 LLLQVMFLYHHH 163
Score = 15.7 bits (28), Expect(3) = 5.0
Identities = 4/6 (66%), Positives = 5/6 (83%)
Frame = +1 / +2
Query: 220 HLIPDH 237
HL+P H
Sbjct: 233 HLLPHH 250
>emb|AJ248322.1|MSA248322 Medicago sativa subsp. X varia mRNA for O-diphenol-O-methyl
transferase (omt gene)
Length = 1346
Score = 24.4 bits (47), Expect = 5.4
Identities = 7/12 (58%), Positives = 10/12 (83%)
Frame = +1 / -3
Query: 34 HLSNHHSNPIFH 69
HL +HH++P FH
Sbjct: 627 HL*SHHTSPTFH 592
>gb|CO513550.1|CO513550 s13dSG12G0900068_139882 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 549
Score = 24.4 bits (47), Expect = 5.4
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = -2 / +3
Query: 188 GPHSNRNNLGKYTAFIWCYL 129
G +N + LGK+ F WC L
Sbjct: 387 GELTNLHLLGKWILFSWCML 446
>gb|CO516580.1|CO516580 s13dSG57H0200016_446488 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 436
Score = 24.4 bits (47), Expect = 5.4
Identities = 5/6 (83%), Positives = 6/6 (100%)
Frame = +2 / +2
Query: 14 NCCSWW 31
NCC+WW
Sbjct: 89 NCCAWW 106
>gb|CO517028.1|CO517028 s13dSG31C0900068_468602 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 501
Score = 20.8 bits (39), Expect(2) = 6.7
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +1 / +1
Query: 22 LLVDHLSNHHSNPIFHIFPLI 84
LL+ LS +PIF I PL+
Sbjct: 82 LLIIILSLFPLHPIFRIHPLL 144
Score = 18.9 bits (35), Expect(2) = 6.7
Identities = 5/7 (71%), Positives = 6/7 (85%)
Frame = +2 / +2
Query: 164 DYFCYCE 184
+YFC CE
Sbjct: 425 NYFCNCE 445
>gb|CO512134.1|CO512134 s13dSG34C0700050_108588 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 543
Score = 20.8 bits (39), Expect(2) = 7.2
Identities = 7/18 (38%), Positives = 10/18 (55%)
Frame = +2 / -1
Query: 167 YFCYCEDHSERC*GKESF 220
+FCY ++HS * F
Sbjct: 231 FFCYNQNHSHHL*SMMMF 178
Score = 18.9 bits (35), Expect(2) = 7.2
Identities = 5/8 (62%), Positives = 7/8 (87%)
Frame = +1 / -1
Query: 46 HHSNPIFH 69
HHS+ +FH
Sbjct: 375 HHSHQMFH 352
>gb|AW698629.1|AW698629 g676 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 238
Score = 24.0 bits (46), Expect = 7.5
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -2 / -3
Query: 194 LNGPHSNRNNLGKYTAFI 141
+NG +SN NNL ++ FI
Sbjct: 59 INGYNSNLNNLWEFCLFI 6
>gb|CO513126.1|CO513126 s13dSG23B0900073_129416 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 130
Score = 24.0 bits (46), Expect = 7.5
Identities = 8/18 (44%), Positives = 13/18 (72%)
Frame = +3 / +2
Query: 60 HISYFSIDSYAKLVVMVL 113
HISYF + +Y+K ++L
Sbjct: 8 HISYFMLTNYSKRFTVLL 61
>gb|CO515118.1|CO515118 s13dSG40D1000090_399101 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 304
Score = 24.0 bits (46), Expect = 7.5
Identities = 5/8 (62%), Positives = 7/8 (87%)
Frame = +2 / +2
Query: 17 CCSWWIIS 40
CC WW++S
Sbjct: 32 CCWWWLVS 55
>gb|DQ122767.1| Medicago sativa clone QC5 serine carboxypeptidase S10 family
protein mRNA, partial cds
Length = 563
Score = 24.0 bits (46), Expect = 7.5
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +1 / -2
Query: 16 LLLLVDHLSNHHSNPIFHIFPL 81
LLL+ H HH+N H PL
Sbjct: 316 LLLIDTHQE*HHTNQYIH*SPL 251
>gb|CO511814.1|CO511814 s13dSG02C1200098_103528 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 578
Score = 20.8 bits (39), Expect(2) = 7.6
Identities = 7/18 (38%), Positives = 10/18 (55%)
Frame = +2 / -1
Query: 167 YFCYCEDHSERC*GKESF 220
+FCY ++HS * F
Sbjct: 233 FFCYNQNHSHHL*SMMMF 180
Score = 18.9 bits (35), Expect(2) = 7.6
Identities = 5/8 (62%), Positives = 7/8 (87%)
Frame = +1 / -1
Query: 46 HHSNPIFH 69
HHS+ +FH
Sbjct: 377 HHSHQMFH 354
>gb|CO513242.1|CO513242 s13dSG24F0600047_129648 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 582
Score = 20.8 bits (39), Expect(2) = 7.7
Identities = 7/18 (38%), Positives = 10/18 (55%)
Frame = +2 / -3
Query: 167 YFCYCEDHSERC*GKESF 220
+FCY ++HS * F
Sbjct: 220 FFCYNQNHSHHL*SMMMF 167
Score = 18.9 bits (35), Expect(2) = 7.7
Identities = 5/8 (62%), Positives = 7/8 (87%)
Frame = +1 / -3
Query: 46 HHSNPIFH 69
HHS+ +FH
Sbjct: 364 HHSHQMFH 341
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: May 2, 2006 2:40 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 2,577,053
Number of Sequences: 7669
Number of extensions: 29209
Number of successful extensions: 3198
Number of sequences better than 10.0: 34
length of database: 1,248,568
effective HSP length: 39
effective length of database: 949,477
effective search space used: 61716005
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)