TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= M73028.2.1
(1802 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512415.1|CO512415 s13dSG80F0600047_114344 Glandular tr... 257 4e-069
gb|CO511707.1|CO511707 s13dSG01B0800073_103314 Glandular tr... 196 6e-051
gb|CO515771.1|CO515771 s13dSG77F1000091_419691 Glandular tr... 161 3e-040
gb|CO514833.1|CO514833 s13dSG59G1100088_328326 Glandular tr... 95 1e-024
gb|CO513208.1|CO513208 s13dSG24B1200093_129580 Glandular tr... 95 2e-023
>gb|CO512415.1|CO512415 s13dSG80F0600047_114344 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 565
Score = 257 bits (555), Expect = 4e-069
Identities = 96/121 (79%), Positives = 110/121 (90%)
Frame = +2 / +2
Query: 320 MGRTPCCEKVGLKRGRWTAEEDQLLANYIAEHGEGSWRSLPKNAGLLRCGKSCRLRWINY 499
MGRTPCCEKVGLKRGRWT EE ++L+ YI +GEGSWRSLPKNAGLLRCGKSCRLRWINY
Sbjct: 164 MGRTPCCEKVGLKRGRWTTEEGEILSKYIQANGEGSWRSLPKNAGLLRCGKSCRLRWINY 343
Query: 500 LRADVKRGNISKEEEDIIIKLHATLGNRWSLIASHLPGRTDNEIKNYWNSHLSRQIHTYR 679
LR D+KRGNIS +EED I+KLH T GNRWS+IASHLPGRTDNEIKN+WNSHLSR+I+++
Sbjct: 344 LRTDLKRGNISAQEEDTIVKLHTTYGNRWSMIASHLPGRTDNEIKNHWNSHLSRKIYSFS 523
Query: 680 R 682
+
Sbjct: 524 K 526
Score = 134 bits (287), Expect = 4e-032
Identities = 65/114 (57%), Positives = 71/114 (62%)
Frame = -3 / -2
Query: 657 LRCEFQ*FLISLSVRPGRWLAIRDHLLPRVAWXXXXXXXXXXXXXXXLTSARR*LIHRSR 478
LR EFQ FLISLSV PG+WLAI DHLLP V W L S R*LIH S
Sbjct: 501 LR*EFQWFLISLSVLPGKWLAIMDHLLP*VVWSFTMVSSS*ADMFPLLRSVLR*LIHLSL 322
Query: 477 QLLPHRSRPAFLGSDLQDPSPCSAM*LASNWSSSAVHLPRLSPTFSQHGVLPIA 316
QL PH + PAF G+DLQ+PSP + M SS VHLP SPTFSQ GVLPI+
Sbjct: 321 QLFPHLNNPAFFGNDLQEPSPLA*MYFDKISPSSVVHLPLFSPTFSQQGVLPIS 160
>gb|CO511707.1|CO511707 s13dSG01B0800073_103314 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 570
Score = 196 bits (423), Expect = 6e-051
Identities = 71/116 (61%), Positives = 90/116 (77%)
Frame = +2 / +1
Query: 320 MGRTPCCEKVGLKRGRWTAEEDQLLANYIAEHGEGSWRSLPKNAGLLRCGKSCRLRWINY 499
MGR PCC+K G+K+G WT EED +L YI EHG G+WR++P GL RC KSCRLRW NY
Sbjct: 13 MGRPPCCDKEGVKKGPWTPEEDIILVTYIQEHGPGNWRAVPTKTGLSRCSKSCRLRWTNY 192
Query: 500 LRADVKRGNISKEEEDIIIKLHATLGNRWSLIASHLPGRTDNEIKNYWNSHLSRQI 667
LR +KRGN +++EE +II L LGNRW+ IAS+LP RTDN+IKNYWN+HL +++
Sbjct: 193 LRPGIKRGNFTEQEEKMIIHLQDLLGNRWAAIASYLPQRTDNDIKNYWNTHLKKKL 360
>gb|CO515771.1|CO515771 s13dSG77F1000091_419691 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 413
Score = 161 bits (346), Expect = 3e-040
Identities = 59/106 (55%), Positives = 81/106 (76%)
Frame = +2 / +3
Query: 350 GLKRGRWTAEEDQLLANYIAEHGEGSWRSLPKNAGLLRCGKSCRLRWINYLRADVKRGNI 529
G+++G WT EED LL I ++GEG W +PK AGL RC KSCRLRW+NYL +KR +
Sbjct: 90 GVRKGAWTYEEDNLLKACINKYGEGKWHLVPKRAGLNRCRKSCRLRWVNYLNPAIKRESF 269
Query: 530 SKEEEDIIIKLHATLGNRWSLIASHLPGRTDNEIKNYWNSHLSRQI 667
S +E D+I++LH LGNRWSLIA+ LPGRT N++KNYW+++L +++
Sbjct: 270 SHDEIDMILRLHKLLGNRWSLIAARLPGRTANDVKNYWHTNLRKKV 407
>gb|CO514833.1|CO514833 s13dSG59G1100088_328326 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 480
Score = 95.5 bits (202), Expect(2) = 1e-024
Identities = 38/68 (55%), Positives = 46/68 (67%)
Frame = +2 / +3
Query: 461 RCGKSCRLRWINYLRADVKRGNISKEEEDIIIKLHATLGNRWSLIASHLPGRTDNEIKNY 640
R GKSCRLRW N L V+ + EE+D II+ HA GN+W+ IA L GRTDN IKN+
Sbjct: 153 RSGKSCRLRWCNQLSPQVEHRAFTPEEDDTIIRAHARFGNKWATIARLLSGRTDNAIKNH 332
Query: 641 WNSHLSRQ 664
WNS L R+
Sbjct: 333 WNSTLKRK 356
Score = 33.1 bits (66), Expect(2) = 1e-024
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +2 / +3
Query: 359 RGRWTAEEDQLLANYIAEHGEGSWRSLPKN 448
+G W+ EED+ L + +HG +W + K+
Sbjct: 54 KGPWSPEEDEALQKLVEKHGPRNWSIISKS 143
>gb|CO513208.1|CO513208 s13dSG24B1200093_129580 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 598
Score = 95.5 bits (202), Expect(2) = 2e-023
Identities = 38/68 (55%), Positives = 46/68 (67%)
Frame = +2 / +3
Query: 461 RCGKSCRLRWINYLRADVKRGNISKEEEDIIIKLHATLGNRWSLIASHLPGRTDNEIKNY 640
R GKSCRLRW N L V+ + EE+D II+ HA GN+W+ IA L GRTDN IKN+
Sbjct: 255 RSGKSCRLRWCNQLSPQVEHRAFTPEEDDTIIRAHARFGNKWATIARLLSGRTDNAIKNH 434
Query: 641 WNSHLSRQ 664
WNS L R+
Sbjct: 435 WNSTLKRK 458
Score = 29.5 bits (58), Expect(2) = 2e-023
Identities = 9/30 (30%), Positives = 17/30 (56%)
Frame = +2 / +3
Query: 359 RGRWTAEEDQLLANYIAEHGEGSWRSLPKN 448
+G W+ EED+ L + +H +W + K+
Sbjct: 156 KGPWSPEEDEALQKLVEKHRPRNWSIISKS 245
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,753,207
Number of Sequences: 7669
Number of extensions: 145109
Number of successful extensions: 1288
Number of sequences better than 1.0e-020: 6
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 506589392
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)