TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= C4H2
(1794 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible c... 451 e-165
gb|CO515318.1|CO515318 s13dSG48E0800067_417695 Glandular tr... 349 7e-097
gb|CO514181.1|CO514181 s13dSG70C1200086_157024 Glandular tr... 225 1e-059
gb|CO513037.1|CO513037 s13dSG09H0900076_122000 Glandular tr... 210 5e-058
gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 87 4e-045
gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 85 1e-044
gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase ... 84 7e-027
gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase ... 84 1e-026
gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase ... 84 8e-026
gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular tr... 104 1e-024
gb|CO516404.1|CO516404 s13dSG27C1100084_446136 Glandular tr... 81 5e-016
gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular tr... 40 3e-015
gb|CO514319.1|CO514319 s13dSG84A1000069_157300 Glandular tr... 61 4e-010
gb|CO515393.1|CO515393 s13dSG49G0500040_417845 Glandular tr... 33 7e-006
gb|CO513758.1|CO513758 s13dSG15G1200088_140298 Glandular tr... 39 0.002
gb|CO513530.1|CO513530 s13dSG12E0600039_139842 Glandular tr... 37 0.008
gb|CO513716.1|CO513716 s13dSG15C0700050_140214 Glandular tr... 36 0.012
gb|CO517155.1|CO517155 s13dSG33C0300022_474394 Glandular tr... 36 0.016
gb|CO514053.1|CO514053 s13dSG72G0600040_156768 Glandular tr... 24 0.46
gb|CO514481.1|CO514481 s13dSG43C0400024_327622 Glandular tr... 29 2.5
gb|CO514097.1|CO514097 s13dSG75C0500034_156856 Glandular tr... 28 3.5
gb|CO512204.1|CO512204 s13dSG03C0400022_113922 Glandular tr... 28 4.8
gb|CO513310.1|CO513310 s13dSG37E0600039_129784 Glandular tr... 28 4.8
gb|CO514948.1|CO514948 s13dSG63D0500046_397401 Glandular tr... 28 4.8
gb|CO515338.1|CO515338 s13dSG48H0200028_417735 Glandular tr... 28 4.8
gb|CO517126.1|CO517126 s13dSG29G0600052_468798 Glandular tr... 28 4.8
gb|CO512424.1|CO512424 s13dSG80G0300020_114362 Glandular tr... 27 9.1
gb|CO512747.1|CO512747 s13dSG88B0900073_121420 Glandular tr... 27 9.1
gb|CO516408.1|CO516408 s13dSG27D0500043_446144 Glandular tr... 27 9.1
gb|CO517095.1|CO517095 s13dSG29C1200098_468736 Glandular tr... 27 9.1
>gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible cinnamic acid 4-hydroxylase
mRNA, complete cds
Length = 1744
Score = 451 bits (979), Expect(3) = e-165
Identities = 183/255 (71%), Positives = 217/255 (85%)
Frame = +2 / +3
Query: 134 VAIAVSKIRGRKLRLXXXXXXXXXFGNWLQVGDDLNHRNLAALSRKFGDVFLLRMGQRNL 313
+A+ +SK+RG++ +L FG WLQVGDDLNHRNL +++FG++FLLRMGQRNL
Sbjct: 84 IAVTISKLRGKRFKLPPGPIPVPIFGYWLQVGDDLNHRNLTDYAKRFGEIFLLRMGQRNL 263
Query: 314 VVVSSPPLAREVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFTVYGDHWRKMRRIMTVPFF 493
VVVSSP LA+EVLHTQ VEFGSRTRNVVFDIFT KGQDMVFTVYG+HWRKMRRIMTVPFF
Sbjct: 264 VVVSSPELAKEVLHTQCVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFF 443
Query: 494 TNKVVQQYRHGWXXXXXXXXXXXRLDPKAATDGIVLRRRLQLMMYNNVYRIMFDRRFESM 673
TNKVVQQYR+GW + + +A+ GIV+R+RLQLMMYN +YRIMFDRRFES
Sbjct: 444 TNKVVQQYRYGWESEAESVVNDVKNNAEASVGGIVIRKRLQLMMYNIMYRIMFDRRFESE 623
Query: 674 DDPLFLRLRALNGERSRLAQSFEYNYGDFIPILRPFLRGYLRVCKEVKETRLKLFKDFFL 853
+DPLF++L+ALNGERSRLAQSFEYNYGDFIPILRPFL+GYL+VCKEVK+ RL+LFKD+F+
Sbjct: 624 EDPLFVKLKALNGERSRLAQSFEYNYGDFIPILRPFLKGYLKVCKEVKDRRLQLFKDYFV 803
Query: 854 EERKKLASTKATDSN 898
+ERKKL STK+T SN
Sbjct: 804 DERKKLESTKSTTSN 848
Score = 295 bits (639), Expect(3) = 3e-087
Identities = 118/155 (76%), Positives = 135/155 (87%)
Frame = +2 / +3
Query: 845 FFLEERKKLASTKATDSNGLKCAIDHILEAQQKGEINEDNVLFIVENINVAAIETTLWSI 1024
F E +K ++ T ++GLKCAIDHIL+AQ+KGEIN+DNVL+IVENINVAAIETTLWSI
Sbjct: 798 FVDERKKLESTKSTTSNDGLKCAIDHILDAQKKGEINDDNVLYIVENINVAAIETTLWSI 977
Query: 1025 EWAVAELVNHPEIQQKLRQELDTVLGPGHQITEPDTHNLPYLQAVIKETLRLRMAIPLLV 1204
EW +AELVNH +IQ K+R+E+D VLGPGHQ+TEPD H LPYLQAVIKETLRLRMAIPLLV
Sbjct: 978 EWGIAELVNHQDIQNKVREEMDRVLGPGHQVTEPDLHKLPYLQAVIKETLRLRMAIPLLV 1157
Query: 1205 PHMNLHDAKLGGYDIPAESKILVNAWYLANNPDSW 1309
PHMNLHD KL G+DIPAESKILVNAW+L NNP W
Sbjct: 1158 PHMNLHDPKLNGFDIPAESKILVNAWWLPNNPAHW 1262
Score = 239 bits (516), Expect(3) = e-154
Identities = 119/227 (52%), Positives = 134/227 (59%)
Frame = -1 / -1
Query: 759 KSP*LYSKLCXXXXXXXXXXXXXRKSGSSMLSKRRSNMIRYTLLYIMSCRRRRSTIPSVA 580
KSP*LYSKL GSS LS SN+I Y +LYI++C TIP
Sbjct: 709 KSP*LYSKL*AKRLLSPFKAFNFTNKGSSSLSNLLSNIILYIILYIINCNLFLITIPPTL 530
Query: 579 ALGXXXXXXXXXXXXXSQPWRYCCTTLLVKKGTVMMRRILRQWSPYTVNTMSCPLSVKMS 400
A S P RYC TTL VKKGTVM+ RILRQ SP TVNTMSCPL VK+S
Sbjct: 529 ASALFLTSLTTLSASDSHP*RYC*TTLFVKKGTVMILRILRQCSP*TVNTMSCPLPVKIS 350
Query: 399 KTTLRVREPNSTPCVWSTSRASGGDETTTRLRCPIRRRKTSPNLRDSAARLRWLRSSPTC 220
TTLRVR+PNST CVW TS AS G+ETTTRLR PIRRRK SP + RLRWLRSSPT
Sbjct: 349 NTTLRVRDPNSTHCVWRTSLASSGEETTTRLRWPIRRRKISPKRLA*SVRLRWLRSSPT* 170
Query: 219 SQFPKXXXXXXXXGSRSLRPRILDTAMATMTDAKRPTRSFSTKRRTM 79
SQ+PK GS PR + A + KR R FS +RR++
Sbjct: 169 SQYPKIGTGIGPGGSLKRLPRSFEIVTAIVAAMKRAKRVFSRRRRSI 29
Score = 204 bits (440), Expect(3) = e-154
Identities = 97/141 (68%), Positives = 106/141 (75%)
Frame = -1 / -1
Query: 1284 YQALTRILLSAGMSXPPSLASWRFMCGTSSGMAIRSRSVSLITACR*GRLCVSGSVIWWP 1105
+ ALT ILLSAG+S P S S RFMCGT SG+AIR RSVSLITACR*G L SGSV W P
Sbjct: 1237 HHALTNILLSAGISKPLSFGS*RFMCGTRSGIAIRRRSVSLITACR*GSLWRSGSVTWCP 1058
Query: 1104 GPSTVSSSCRSFCWISGWFTSSATAHSIDHSVVSIAATLMFSTMKSTLSSLISPFCCASS 925
GP T+S S + W+S WFTSSA HSIDHSVVSIAATLMFSTM TLSSLISPF AS
Sbjct: 1057 GPRTLSISSLTLFWMSWWFTSSAIPHSIDHSVVSIAATLMFSTM*RTLSSLISPFF*ASK 878
Query: 924 M*SMAHLRPLLSVALVLASFF 862
M*S+AH P L V L++ S F
Sbjct: 877 M*SIAHFSPSLLVVLLVLSSF 815
Score = 143 bits (307), Expect(3) = e-165
Identities = 59/80 (73%), Positives = 62/80 (77%)
Frame = +2 / +3
Query: 1358 HVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQDKVDTTEKGG 1537
HVEANGNDFRYLPFGVGRRSCP RLVQN ELLPPPGQ K+DT+EKGG
Sbjct: 1311 HVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNVELLPPPGQSKIDTSEKGG 1490
Query: 1538 QFSLHILKHSTIVCKPRTL* 1597
QFSLHILKHSTIV KPR+ *
Sbjct: 1491 QFSLHILKHSTIVAKPRSF* 1550
Score = 138 bits (295), Expect(3) = e-154
Identities = 59/83 (71%), Positives = 66/83 (79%)
Frame = -1 / -1
Query: 1608 VGCS*SVLGLHTMVECFKMWRLNWPPFSVVSTLSCPGGGSSSKFWTRRPMVMPRMGRARM 1429
V +*+ LGL TMVEC + RLN PPFS VS CPGGG SS F TRRP+V+PR+G+ARM
Sbjct: 1561 VKTN*NDLGLATMVECLSICRLNCPPFSEVSIFDCPGGGKSSTF*TRRPIVIPRIGKARM 1382
Query: 1428 IPGQLLRPTPKGRYLKSLPLAST 1360
IPGQLL PTPKGRYLKS PLAST
Sbjct: 1381 IPGQLLLPTPKGRYLKSFPLAST 1313
Score = 101 bits (216), Expect(3) = 4e-028
Identities = 55/143 (38%), Positives = 76/143 (53%)
Frame = -3 / -3
Query: 508 HDLVGEEGHGHDAAHLAPVVAVHGEHHVLPLVREDVEDHVAGAGAELHALRVEHLPRQRR 329
++LV EE +GHD+AH P+ AV EHHVL E++E +V +E + L VE +
Sbjct: 458 NNLVCEERNGHDSAHFTPMFAVDSEHHVLSFTCENIEYYVTCT*SEFNTLCVEDFFGEFW 279
Query: 328 RRDHHQVALPHPEEEDVAELAGQRGEVAVVEVVADLQPVPEDGHGDGAGRQPELAATDLX 149
R++ V L H EED E G GEV VVE+++DL+PV ED D + E AT+
Sbjct: 278 GRNYD*VTLAHT*EEDFTEALGVIGEVTVVEIISDLKPVSEDRDWDWTWWEFEAFATEF* 99
Query: 148 XXXXXXXXRQEADQELLHEEKDH 80
+E + L EEK H
Sbjct: 98 DCDGDCGGNEEG*KGLFKEEKIH 30
Score = 47.8 bits (98), Expect(3) = 3e-087
Identities = 19/44 (43%), Positives = 29/44 (65%)
Frame = +1 / +2
Query: 370 VRLPHPQRGLRHLHGQGAGHGVHRVRRPLAQDAPHHDRALLHQQ 501
+R+ + R +R+ H HGVH +RR LA +A +HDR+ LH+Q
Sbjct: 320 IRITYT*RSIRYFHR*RTRHGVHCLRRTLA*NAQNHDRSFLHKQ 451
Score = 34.1 bits (68), Expect(3) = 4e-028
Identities = 15/37 (40%), Positives = 22/37 (59%)
Frame = -3 / -3
Query: 1261 ALGGDVVXAELGVVEVHVRHQQRDGHPQPQRLLDHRL 1151
ALG D+ EL +++VHV ++ D H + L DH L
Sbjct: 1214 ALGWDIKTIELWIMKVHVWDEEWDRHS*TKCLFDHSL 1104
Score = 30.9 bits (61), Expect(3) = 4e-028
Identities = 9/33 (27%), Positives = 21/33 (63%)
Frame = -3 / -3
Query: 667 LEAPVEHDPVYVVVHHELQASAEHDSVGGRLGV 569
L++ ++H+P++ ++HH+L ++ R GV
Sbjct: 617 LKSSIKHNPIHYIIHHQL*PLPYNNPTNTRFGV 519
Score = 30.9 bits (61), Expect(3) = e-165
Identities = 16/37 (43%), Positives = 25/37 (67%)
Frame = +1 / +2
Query: 913 H*SHTGGTAEG*DQRGQRALHRREH*RCSDRDHAVVD 1023
+*SH G + EG*+Q QR+LH EH C + ++ +V+
Sbjct: 866 Y*SHFGCSKEG*NQ**QRSLHC*EHQCCCN*NNTMVN 976
Score = 22.6 bits (43), Expect(3) = 3e-087
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = +1 / +2
Query: 1519 HHREGRPVQSPHLEAFHHRVQAKNALRAAHTS 1614
H E R V S + + F+H AK L + HT+
Sbjct: 1472 HF*ERRTV*SAYTQTFNHCC*AKIILISLHTN 1567
>gb|CO515318.1|CO515318 s13dSG48E0800067_417695 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 557
Score = 349 bits (756), Expect = 7e-097
Identities = 142/184 (77%), Positives = 161/184 (87%)
Frame = +2 / +1
Query: 221 QVGDDLNHRNLAALSRKFGDVFLLRMGQRNLVVVSSPPLAREVLHTQGVEFGSRTRNVVF 400
QVGDDLNHRNL +++FG++FLLRMGQRNLVVVSSP LA+EVLHTQGVEFGSRTRNVVF
Sbjct: 4 QVGDDLNHRNLTDYAKRFGEIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVF 183
Query: 401 DIFTDKGQDMVFTVYGDHWRKMRRIMTVPFFTNKVVQQYRHGWXXXXXXXXXXXRLDPKA 580
DIFT KGQDMVFTVYG+HWRKMRRIMTVPFFTNKVVQQYR+GW + + +A
Sbjct: 184 DIFTSKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRYGWESEAESVVNDVKNNAEA 363
Query: 581 ATDGIVLRRRLQLMMYNNVYRIMFDRRFESMDDPLFLRLRALNGERSRLAQSFEYNYGDF 760
+ GIV+R+RLQLMMYN +YRIMFDRRFES +DPLF++L+ALNGERSRLA SFEYNYGDF
Sbjct: 364 SVGGIVIRKRLQLMMYNIMYRIMFDRRFESEEDPLFVKLKALNGERSRLAXSFEYNYGDF 543
Query: 761 IPIL 772
IPIL
Sbjct: 544 IPIL 555
Score = 223 bits (481), Expect = 6e-059
Identities = 106/180 (58%), Positives = 114/180 (63%)
Frame = -1 / -1
Query: 759 KSP*LYSKLCXXXXXXXXXXXXXRKSGSSMLSKRRSNMIRYTLLYIMSCRRRRSTIPSVA 580
KSP*LYSKL GSS LS SN+I Y +LYI++C TIP
Sbjct: 542 KSP*LYSKL*AKRLLSPFKAFNFTNKGSSSLSNLLSNIILYIILYIINCNLFLITIPPTL 363
Query: 579 ALGXXXXXXXXXXXXXSQPWRYCCTTLLVKKGTVMMRRILRQWSPYTVNTMSCPLSVKMS 400
A S P RYC TTL VK GTVM+ RILRQ SP TVNTMSCPL VKMS
Sbjct: 362 ASALFLTSLTTLSASDSHP*RYC*TTLFVKNGTVMILRILRQCSP*TVNTMSCPLLVKMS 183
Query: 399 KTTLRVREPNSTPCVWSTSRASGGDETTTRLRCPIRRRKTSPNLRDSAARLRWLRSSPTC 220
KTTLRVR+PNSTPCVW TS AS G+ETTTRLR PIRRRK SP + RLRWLRSSPTC
Sbjct: 182 KTTLRVRDPNSTPCVWRTSLASSGEETTTRLR*PIRRRKISPKRLA*SVRLRWLRSSPTC 3
Score = 89.0 bits (188), Expect(2) = 2e-020
Identities = 43/96 (44%), Positives = 58/96 (60%)
Frame = -3 / -3
Query: 508 HDLVGEEGHGHDAAHLAPVVAVHGEHHVLPLVREDVEDHVAGAGAELHALRVEHLPRQRR 329
++LV EE +GHD+ H P+ AV EHHVL EDVE++V +E + L VE +
Sbjct: 291 NNLVCEERNGHDSTHFTPMFAVDSEHHVLSFTCEDVENYVTCT*SEFNTLCVEDFFGEFW 112
Query: 328 RRDHHQVALPHPEEEDVAELAGQRGEVAVVEVVADL 221
R++ V L H EED AE G GEV VVE+++DL
Sbjct: 111 GRNYD*VTLTHT*EEDFAEAFGVIGEVTVVEIISDL 4
Score = 50.6 bits (104), Expect = 6e-007
Identities = 21/44 (47%), Positives = 30/44 (68%)
Frame = +1 / +3
Query: 370 VRLPHPQRGLRHLHGQGAGHGVHRVRRPLAQDAPHHDRALLHQQ 501
+R+ + R RHLH HGVH +RR LA +A +HDR++LH+Q
Sbjct: 153 IRITYT*RSFRHLHK*RTRHGVHCLRRTLA*NA*NHDRSVLHKQ 284
Score = 25.4 bits (49), Expect(2) = 2e-020
Identities = 7/32 (21%), Positives = 19/32 (59%)
Frame = -3 / -3
Query: 664 EAPVEHDPVYVVVHHELQASAEHDSVGGRLGV 569
++ ++H+ ++ ++HH+L ++ R GV
Sbjct: 447 KSSIKHNSIHYIIHHQL*PLPYNNPTNTRFGV 352
>gb|CO514181.1|CO514181 s13dSG70C1200086_157024 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 358
Score = 225 bits (486), Expect = 1e-059
Identities = 91/116 (78%), Positives = 104/116 (89%)
Frame = +2 / +1
Query: 887 TDSNGLKCAIDHILEAQQKGEINEDNVLFIVENINVAAIETTLWSIEWAVAELVNHPEIQ 1066
T ++GLKCAIDHIL+AQ+KGEIN +VL+I ENIN AAIETTLW+IEW +AELVNH +IQ
Sbjct: 10 TSNDGLKCAIDHILDAQKKGEINGGDVLYIGENINGAAIETTLWAIEWGIAELVNHQDIQ 189
Query: 1067 QKLRQELDTVLGPGHQITEPDTHNLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKL 1234
K+R+E+D VLGPGHQ+TEPD H LPYLQA IKETLRLRMAIPLLVPHMNLHDAKL
Sbjct: 190 NKVREEMDRVLGPGHQVTEPDLHKLPYLQAGIKETLRLRMAIPLLVPHMNLHDAKL 357
Score = 158 bits (340), Expect = 2e-039
Identities = 77/116 (66%), Positives = 82/116 (70%)
Frame = -1 / -3
Query: 1233 SLASWRFMCGTSSGMAIRSRSVSLITACR*GRLCVSGSVIWWPGPSTVSSSCRSFCWISG 1054
S AS RFMCGT SG+AIR RSVSLI ACR*G L SGSV W PGP T+S S + W+S
Sbjct: 356 SFAS*RFMCGTRSGIAIRRRSVSLIPACR*GSLWRSGSVTWCPGPRTLSISSLTLFWMSW 177
Query: 1053 WFTSSATAHSIDHSVVSIAATLMFSTMKSTLSSLISPFCCASSM*SMAHLRPLLSV 886
WFTSSA HSI HSVVSIAA LMFS M T LISPF AS M*S+AH P L V
Sbjct: 176 WFTSSAIPHSIAHSVVSIAAPLMFSPM*RTSPPLISPFF*ASKM*SIAHFSPSLLV 9
Score = 32.2 bits (64), Expect = 0.20
Identities = 15/28 (53%), Positives = 20/28 (71%)
Frame = +1 / +3
Query: 913 H*SHTGGTAEG*DQRGQRALHRREH*RC 996
+*SH G + EG*+Q G+R+LH EH C
Sbjct: 36 Y*SHFGCSKEG*NQWGRRSLHW*EHQWC 119
>gb|CO513037.1|CO513037 s13dSG09H0900076_122000 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 526
Score = 210 bits (452), Expect(2) = 5e-058
Identities = 85/125 (68%), Positives = 104/125 (83%)
Frame = +2 / +3
Query: 524 GWXXXXXXXXXXXRLDPKAATDGIVLRRRLQLMMYNNVYRIMFDRRFESMDDPLFLRLRA 703
GW + + +A+ GIV+ +RLQLMMYN +YRIMFDRRFES +DPLF++L+A
Sbjct: 6 GWESEAESVVNDVKNNAEASVGGIVIWKRLQLMMYNIMYRIMFDRRFESEEDPLFVKLKA 185
Query: 704 LNGERSRLAQSFEYNYGDFIPILRPFLRGYLRVCKEVKETRLKLFKDFFLEERKKLASTK 883
LNGERSRLAQSFEYNYGDFIPILRPFL+GYL+VCKEVK+ RL+LFKD+F++ERKKL STK
Sbjct: 186 LNGERSRLAQSFEYNYGDFIPILRPFLKGYLKVCKEVKDRRLQLFKDYFVDERKKLESTK 365
Query: 884 ATDSN 898
+T SN
Sbjct: 366 STTSN 380
Score = 112 bits (239), Expect = 1e-025
Identities = 45/65 (69%), Positives = 55/65 (84%)
Frame = +2 / +3
Query: 845 FFLEERKKLASTKATDSNGLKCAIDHILEAQQKGEINEDNVLFIVENINVAAIETTLWSI 1024
F E +K ++ T ++GLKCAIDHIL+AQ+KGEIN+DNVL+IVENINVAAIETTLWSI
Sbjct: 330 FVDERKKLESTKSTTSNDGLKCAIDHILDAQKKGEINDDNVLYIVENINVAAIETTLWSI 509
Query: 1025 EWAVA 1039
EW +A
Sbjct: 510 EWGIA 524
Score = 86.8 bits (183), Expect(2) = 9e-027
Identities = 43/60 (71%), Positives = 46/60 (76%)
Frame = -1 / -1
Query: 1041 SATAHSIDHSVVSIAATLMFSTMKSTLSSLISPFCCASSM*SMAHLRPLLSVALVLASFF 862
SA HSIDHSVVSIAATLMFSTM TLSSLISPF AS M*S+AH P L V L++ S F
Sbjct: 526 SAIPHSIDHSVVSIAATLMFSTM*RTLSSLISPFF*ASKM*SIAHFSPSLLVVLLVLSSF 347
Score = 49.2 bits (101), Expect(2) = 9e-027
Identities = 26/61 (42%), Positives = 30/61 (49%)
Frame = -1 / -1
Query: 759 KSP*LYSKLCXXXXXXXXXXXXXRKSGSSMLSKRRSNMIRYTLLYIMSCRRRRSTIPSVA 580
KSP*LYSKL GSS LS SN+I Y +LYI++C TIP
Sbjct: 241 KSP*LYSKL*AKRLLSPFKAFNFTNKGSSSLSNLLSNIILYIILYIINCNLFHITIPPTL 62
Query: 579 A 577
A
Sbjct: 61 A 59
Score = 30.9 bits (61), Expect(2) = 5e-058
Identities = 16/37 (43%), Positives = 25/37 (67%)
Frame = +1 / +2
Query: 913 H*SHTGGTAEG*DQRGQRALHRREH*RCSDRDHAVVD 1023
+*SH G + EG*+Q QR+LH EH C + ++ +V+
Sbjct: 398 Y*SHFGCSKEG*NQ**QRSLHC*EHQCCCN*NNTMVN 508
Score = 30.9 bits (61), Expect = 0.52
Identities = 9/33 (27%), Positives = 21/33 (63%)
Frame = -3 / -3
Query: 667 LEAPVEHDPVYVVVHHELQASAEHDSVGGRLGV 569
L++ ++H+P++ ++HH+L ++ R GV
Sbjct: 149 LKSSIKHNPIHYIIHHQL*PLPYNNPTNTRFGV 51
>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
Length = 1885
Score = 87.2 bits (184), Expect(4) = 4e-045
Identities = 37/86 (43%), Positives = 55/86 (63%)
Frame = +2 / +2
Query: 947 EINEDNVLFIVENINVAAIETTLWSIEWAVAELVNHPEIQQKLRQELDTVLGPGHQITEP 1126
++ +DN+ I+ ++ ET +IEWA+AEL+ PE +K++QEL V+G Q+ EP
Sbjct: 977 KLTKDNIKAIIMDVMFGGTETVASAIEWAMAELMKSPEDLKKVQQELAEVVGLSRQVEEP 1156
Query: 1127 DTHNLPYLQAVIKETLRLRMAIPLLV 1204
D L YL+ +KETLRL IPLL+
Sbjct: 1157 DFEKLTYLKCALKETLRLHPPIPLLL 1234
Score = 84.0 bits (177), Expect(4) = 4e-045
Identities = 36/97 (37%), Positives = 53/97 (54%)
Frame = +2 / +2
Query: 230 DDLNHRNLAALSRKFGDVFLLRMGQRNLVVVSSPPLAREVLHTQGVEFGSRTRNVVFDIF 409
D L HR LA L++K+G +F LRMG ++V +S AR+VL Q F +R V
Sbjct: 257 DQLTHRGLANLAKKYGGIFHLRMGFLHMVAISDADAARQVLQVQDNIFSNRPATVAIKYL 436
Query: 410 TDKGQDMVFTVYGDHWRKMRRIMTVPFFTNKVVQQYR 520
T DM F YG WR+MR++ + F+ K + ++
Sbjct: 437 TYDRADMAFAHYGPFWRQMRKLCVMKLFSRKHAESWQ 547
Score = 46.9 bits (96), Expect(3) = 6e-015
Identities = 34/92 (36%), Positives = 43/92 (46%)
Frame = -1 / -2
Query: 1200 SSGMAIRSRSVSLITACR*GRLCVSGSVIWWPGPSTVSSSCRSFCWISGWFTSSATAHSI 1021
S G+ R VSL R* SGS P+T +SSC +F SG F +SA AHSI
Sbjct: 1230 SKGIGG*RRRVSLRAHFR*VSFSKSGSSTCRLRPTTSASSCWTFLRSSGLFINSAIAHSI 1051
Query: 1020 DHSVVSIAATLMFSTMKSTLSSLISPFCCASS 925
+ VS+ + M LS + C SS
Sbjct: 1050 ADATVSVPPNITSIMMALMLSLVSLMELCKSS 955
Score = 44.6 bits (91), Expect(3) = 6e-015
Identities = 28/80 (35%), Positives = 33/80 (41%)
Frame = -1 / -2
Query: 468 RILRQWSPYTVNTMSCPLSVKMSKTTLRVREPNSTPCVWSTSRASGGDETTTRLRCPIRR 289
RI RQ P N MS V+ T+ R W T RA+ E T R P+RR
Sbjct: 495 RIWRQKGP**ANAMSARS*VRYLMATVAGRLEKMLS*TWRTCRAASASEIATMWRNPMRR 316
Query: 288 RKTSPNLRDSAARLRWLRSS 229
K P AR RW+ S
Sbjct: 315 *KMPPYFLAKLARPRWVNWS 256
Score = 38.6 bits (78), Expect(4) = 4e-045
Identities = 11/37 (29%), Positives = 23/37 (62%)
Frame = +2 / +2
Query: 1199 LVPHMNLHDAKLGGYDIPAESKILVNAWYLANNPDSW 1309
L+ H +A + GY IP ++++++NAW + + + W
Sbjct: 1226 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCW 1336
Score = 35.9 bits (72), Expect(4) = 4e-045
Identities = 11/17 (64%), Positives = 15/17 (88%)
Frame = +2 / +2
Query: 1373 GNDFRYLPFGVGRRSCP 1423
G++F ++PFG GRRSCP
Sbjct: 1397 GSNFEFIPFGSGRRSCP 1447
Score = 30.4 bits (60), Expect(3) = 6e-015
Identities = 14/22 (63%), Positives = 14/22 (63%)
Frame = -1 / -2
Query: 1428 IPGQLLRPTPKGRYLKSLPLAS 1363
IPGQ LRP P G K LPL S
Sbjct: 1452 IPGQDLRPDPNGINSKLLPLKS 1387
>gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-K1) mRNA, complete cds
Length = 1904
Score = 85.4 bits (180), Expect(4) = 1e-044
Identities = 36/86 (41%), Positives = 54/86 (62%)
Frame = +2 / +1
Query: 947 EINEDNVLFIVENINVAAIETTLWSIEWAVAELVNHPEIQQKLRQELDTVLGPGHQITEP 1126
++ +DN+ I+ ++ ET +IEW +AEL+ PE +K++QEL V+G Q+ EP
Sbjct: 991 KLTKDNIKAIIMDVMFGGTETVASAIEWVMAELMKSPEDLKKVQQELAEVVGLSRQVEEP 1170
Query: 1127 DTHNLPYLQAVIKETLRLRMAIPLLV 1204
D L YL+ +KETLRL IPLL+
Sbjct: 1171 DFEKLTYLKCALKETLRLHPPIPLLL 1248
Score = 84.0 bits (177), Expect(4) = 1e-044
Identities = 36/97 (37%), Positives = 53/97 (54%)
Frame = +2 / +1
Query: 230 DDLNHRNLAALSRKFGDVFLLRMGQRNLVVVSSPPLAREVLHTQGVEFGSRTRNVVFDIF 409
D L HR LA L++K+G +F LRMG ++V +S AR+VL Q F +R V
Sbjct: 271 DQLTHRGLANLAKKYGGIFHLRMGFLHMVAISDADAARQVLQVQDNIFSNRPATVAIKYL 450
Query: 410 TDKGQDMVFTVYGDHWRKMRRIMTVPFFTNKVVQQYR 520
T DM F YG WR+MR++ + F+ K + ++
Sbjct: 451 TYDRADMAFAHYGPFWRQMRKLCVMKLFSRKHAESWQ 561
Score = 44.1 bits (90), Expect(3) = 7e-014
Identities = 33/92 (35%), Positives = 42/92 (45%)
Frame = -1 / -1
Query: 1200 SSGMAIRSRSVSLITACR*GRLCVSGSVIWWPGPSTVSSSCRSFCWISGWFTSSATAHSI 1021
S G+ R VSL R* SGS P+T +SSC +F SG F +SA HSI
Sbjct: 1244 SKGIGG*RRRVSLRAHFR*VSFSKSGSSTCRLRPTTSASSC*TFFRSSGLFINSAITHSI 1065
Query: 1020 DHSVVSIAATLMFSTMKSTLSSLISPFCCASS 925
+ VS+ + M LS + C SS
Sbjct: 1064 ADATVSVPPNITSIMMALMLSLVSLMELCKSS 969
Score = 43.7 bits (89), Expect(3) = 7e-014
Identities = 28/80 (35%), Positives = 33/80 (41%)
Frame = -1 / -1
Query: 468 RILRQWSPYTVNTMSCPLSVKMSKTTLRVREPNSTPCVWSTSRASGGDETTTRLRCPIRR 289
RI RQ P N MS V+ T+ R W T RA+ E T R P+RR
Sbjct: 509 RIWRQKGP**ANAMSARS*VRYLIATVAGRLEKMLS*TWRTCRAASASEIATMWRNPMRR 330
Query: 288 RKTSPNLRDSAARLRWLRSS 229
K P AR RW+ S
Sbjct: 329 *KMPPYFLAKLARPRWVNWS 270
Score = 38.6 bits (78), Expect(4) = 1e-044
Identities = 11/37 (29%), Positives = 23/37 (62%)
Frame = +2 / +1
Query: 1199 LVPHMNLHDAKLGGYDIPAESKILVNAWYLANNPDSW 1309
L+ H +A + GY IP ++++++NAW + + + W
Sbjct: 1240 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCW 1350
Score = 35.9 bits (72), Expect(4) = 1e-044
Identities = 11/17 (64%), Positives = 15/17 (88%)
Frame = +2 / +1
Query: 1373 GNDFRYLPFGVGRRSCP 1423
G++F ++PFG GRRSCP
Sbjct: 1411 GSNFEFIPFGSGRRSCP 1461
Score = 30.4 bits (60), Expect(3) = 7e-014
Identities = 14/22 (63%), Positives = 14/22 (63%)
Frame = -1 / -1
Query: 1428 IPGQLLRPTPKGRYLKSLPLAS 1363
IPGQ LRP P G K LPL S
Sbjct: 1466 IPGQDLRPDPNGINSKLLPLKS 1401
>gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase 3 (ifs3) mRNA, partial cds
Length = 1501
Score = 84.5 bits (178), Expect(3) = 7e-027
Identities = 31/85 (36%), Positives = 59/85 (69%)
Frame = +2 / +3
Query: 947 EINEDNVLFIVENINVAAIETTLWSIEWAVAELVNHPEIQQKLRQELDTVLGPGHQITEP 1126
+I +D++ +V + A ++T + EWA+AEL+N+P++ +K R+E+ +V+G + E
Sbjct: 822 KITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVVGKDRLVDEV 1001
Query: 1127 DTHNLPYLQAVIKETLRLRMAIPLL 1201
DT NLPY++A++KET R+ +P++
Sbjct: 1002 DTQNLPYIRAIVKETFRMHPPLPVV 1076
Score = 40.0 bits (81), Expect(2) = 2e-006
Identities = 25/61 (40%), Positives = 33/61 (54%)
Frame = -1 / -1
Query: 1176 RSVSLITACR*GRLCVSGSVIWWPGPSTVSSSCRSFCWISGWFTSSATAHSIDHSVVSIA 997
R+VS A *GR VS S P+T+ +S R+F G SSA AHS+ +V S+
Sbjct: 1051 RNVSFTIALM*GRF*VSTSSTSLSFPTTL*TSSRAFSNTLGLLMSSANAHSVATAVESVP 872
Query: 996 A 994
A
Sbjct: 871 A 869
Score = 38.2 bits (77), Expect(3) = 7e-027
Identities = 15/43 (34%), Positives = 20/43 (46%)
Frame = +2 / +3
Query: 1361 VEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFEL 1489
++ G F+ LPFG GRR CP L+Q F+L
Sbjct: 1248 LDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDL 1376
Score = 31.3 bits (62), Expect(3) = 7e-027
Identities = 10/29 (34%), Positives = 16/29 (55%)
Frame = +2 / +3
Query: 1223 DAKLGGYDIPAESKILVNAWYLANNPDSW 1309
+ ++ GY IP + IL N W + +P W
Sbjct: 1095 ECEINGYVIPEGALILFNVWQVGRDPKYW 1181
Score = 31.3 bits (62), Expect(2) = 2e-006
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = -1 / -1
Query: 1437 ARMIPGQLLRPTPKGRYLKSLPLAS 1363
AR+ PG +L P P GR K PL S
Sbjct: 1324 ARLTPGHILLPDPNGRS*KCCPLRS 1250
>gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase 1 (ifs1) mRNA, partial cds
Length = 1501
Score = 84.0 bits (177), Expect(3) = 1e-026
Identities = 31/85 (36%), Positives = 58/85 (68%)
Frame = +2 / +3
Query: 947 EINEDNVLFIVENINVAAIETTLWSIEWAVAELVNHPEIQQKLRQELDTVLGPGHQITEP 1126
+I ++ + +V ++ A ++T + EWA+AEL+N+P + QK R+E+ +V+G + E
Sbjct: 822 KITKEQIKGLVVDLFSAGTDSTAVATEWALAELINNPRVLQKAREEVYSVVGKDRLVDEV 1001
Query: 1127 DTHNLPYLQAVIKETLRLRMAIPLL 1201
DT NLPY++A++KET R+ +P++
Sbjct: 1002 DTQNLPYIRAIVKETFRMHPPLPVV 1076
Score = 44.6 bits (91), Expect(2) = 2e-007
Identities = 29/76 (38%), Positives = 39/76 (51%)
Frame = -1 / -1
Query: 1176 RSVSLITACR*GRLCVSGSVIWWPGPSTVSSSCRSFCWISGWFTSSATAHSIDHSVVSIA 997
R+VS A *GR VS S P+T+ +S R+FC G SSA AHS+ +V S+
Sbjct: 1051 RNVSFTMALM*GRF*VSTSSTSLSLPTTL*TSSRAFCNTLGLLMSSANAHSVATAVESVP 872
Query: 996 ATLMFSTMKSTLSSLI 949
A +T S +I
Sbjct: 871 AEKRSTTRPLICSLVI 824
Score = 38.2 bits (77), Expect(3) = 1e-026
Identities = 15/43 (34%), Positives = 20/43 (46%)
Frame = +2 / +3
Query: 1361 VEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFEL 1489
++ G F+ LPFG GRR CP L+Q F+L
Sbjct: 1248 LDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDL 1376
Score = 30.9 bits (61), Expect(3) = 1e-026
Identities = 9/29 (31%), Positives = 16/29 (55%)
Frame = +2 / +3
Query: 1223 DAKLGGYDIPAESKILVNAWYLANNPDSW 1309
+ ++ GY IP + +L N W + +P W
Sbjct: 1095 ECEINGYVIPEGALVLFNVWQVGRDPKYW 1181
Score = 30.4 bits (60), Expect(2) = 2e-007
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1 / -1
Query: 1437 ARMIPGQLLRPTPKGRYLKSLPLAS 1363
A++ PG +L P P GR K PL S
Sbjct: 1324 AKLTPGHILLPDPNGRSWKCWPLRS 1250
>gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase 2 (ifs2) mRNA, partial cds
Length = 1501
Score = 84.5 bits (178), Expect(3) = 8e-026
Identities = 31/85 (36%), Positives = 59/85 (69%)
Frame = +2 / +3
Query: 947 EINEDNVLFIVENINVAAIETTLWSIEWAVAELVNHPEIQQKLRQELDTVLGPGHQITEP 1126
+I +D++ +V + A ++T + EWA+AEL+N+P++ +K R+E+ +V+G + E
Sbjct: 822 KITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVVGKDRLVDEV 1001
Query: 1127 DTHNLPYLQAVIKETLRLRMAIPLL 1201
DT NLPY++A++KET R+ +P++
Sbjct: 1002 DTQNLPYIRAIVKETFRMHPPLPVV 1076
Score = 40.0 bits (81), Expect(2) = 2e-006
Identities = 25/61 (40%), Positives = 33/61 (54%)
Frame = -1 / -1
Query: 1176 RSVSLITACR*GRLCVSGSVIWWPGPSTVSSSCRSFCWISGWFTSSATAHSIDHSVVSIA 997
R+VS A *GR VS S P+T+ +S R+F G SSA AHS+ +V S+
Sbjct: 1051 RNVSFTIALM*GRF*VSTSSTSLSFPTTL*TSSRAFSNTLGLLMSSANAHSVATAVESVP 872
Query: 996 A 994
A
Sbjct: 871 A 869
Score = 38.2 bits (77), Expect(3) = 8e-026
Identities = 15/43 (34%), Positives = 20/43 (46%)
Frame = +2 / +3
Query: 1361 VEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFEL 1489
++ G F+ LPFG GRR CP L+Q F+L
Sbjct: 1248 LDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDL 1376
Score = 31.3 bits (62), Expect(2) = 2e-006
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = -1 / -1
Query: 1437 ARMIPGQLLRPTPKGRYLKSLPLAS 1363
AR+ PG +L P P GR K PL S
Sbjct: 1324 ARLTPGHILLPDPNGRS*KCCPLRS 1250
Score = 27.6 bits (54), Expect(3) = 8e-026
Identities = 9/29 (31%), Positives = 15/29 (51%)
Frame = +2 / +3
Query: 1223 DAKLGGYDIPAESKILVNAWYLANNPDSW 1309
+ ++ GY IP + IL N W + + W
Sbjct: 1095 ECEINGYVIPEGALILFNVWQVGRDSKYW 1181
>gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 506
Score = 104 bits (222), Expect(2) = 1e-024
Identities = 39/114 (34%), Positives = 73/114 (64%)
Frame = +2 / +2
Query: 968 LFIVENINVAAIETTLWSIEWAVAELVNHPEIQQKLRQELDTVLGPGHQITEPDTHNLPY 1147
L ++++ V E++ + WA++E+V +P + +K + E+ V + E + H L Y
Sbjct: 74 LIHLQDMFVGGTESSSEVVLWAMSEMVKNPMVMEKAQVEVRRVFDKKGYVDETELHQLIY 253
Query: 1148 LQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWYLANNPDSW 1309
L++VIKET+RL ++PLL+P ++ ++ GYDIPA+++++VNAW + +P W
Sbjct: 254 LKSVIKETMRLHPSLPLLIPRESIKPCQINGYDIPAKTRVIVNAWAIGRDPRYW 415
Score = 34.5 bits (69), Expect = 0.041
Identities = 30/99 (30%), Positives = 46/99 (46%)
Frame = -1 / -3
Query: 1281 QALTRILLSAGMSXPPSLASWRFMCGTSSGMAIRSRSVSLITACR*GRLCVSGSVIWWPG 1102
QALT L+ AG+S P + G ++G + VSL+T + C S S +
Sbjct: 387 QALTMTLVLAGISYPFIWHGFILSLGINNGKEGCNLIVSLMTDFKYINWCNSVSSTYPFL 208
Query: 1101 PSTVSSSCRSFCWISGWFTSSATAHSIDHSVVSIAATLM 985
+T+ +S +F G+FT S AH+ S+ T M
Sbjct: 207 SNTLLTSTCAFSITIGFFTISDIAHNTTSEDDSVPPTNM 91
Score = 24.0 bits (46), Expect(2) = 1e-024
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +2 / +2
Query: 1373 GNDFRYLPFG 1402
G DF Y+PFG
Sbjct: 473 GTDFEYIPFG 502
>gb|CO516404.1|CO516404 s13dSG27C1100084_446136 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 534
Score = 80.8 bits (170), Expect = 5e-016
Identities = 32/99 (32%), Positives = 57/99 (57%)
Frame = +2 / +3
Query: 224 VGDDLNHRNLAALSRKFGDVFLLRMGQRNLVVVSSPPLAREVLHTQGVEFGSRTRNVVFD 403
+ L H L+ K+G + L++G+ + ++VSS +A+EVL T + F +R ++
Sbjct: 189 ISSSLPHHGFKNLADKYGPLMHLKLGEISYIIVSSSEMAKEVLKTHDLTFCNRPNLLLST 368
Query: 404 IFTDKGQDMVFTVYGDHWRKMRRIMTVPFFTNKVVQQYR 520
I + +D+ F+ YG+HWR++R+I V K VQ +R
Sbjct: 369 ILSYNARDIAFSTYGEHWRQLRKICVVELLNAKRVQSFR 485
>gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 39.6 bits (80), Expect(3) = 3e-015
Identities = 16/30 (53%), Positives = 20/30 (66%)
Frame = +2 / +2
Query: 1115 ITEPDTHNLPYLQAVIKETLRLRMAIPLLV 1204
+ EPD L YL+ +KETLRL IPLL+
Sbjct: 5 VEEPDFEKLTYLKCALKETLRLHPPIPLLL 94
Score = 38.6 bits (78), Expect(3) = 3e-015
Identities = 11/37 (29%), Positives = 23/37 (62%)
Frame = +2 / +2
Query: 1199 LVPHMNLHDAKLGGYDIPAESKILVNAWYLANNPDSW 1309
L+ H +A + GY IP ++++++NAW + + + W
Sbjct: 86 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCW 196
Score = 35.9 bits (72), Expect(3) = 3e-015
Identities = 11/17 (64%), Positives = 15/17 (88%)
Frame = +2 / +2
Query: 1373 GNDFRYLPFGVGRRSCP 1423
G++F ++PFG GRRSCP
Sbjct: 257 GSNFEFIPFGSGRRSCP 307
Score = 30.4 bits (60), Expect = 0.71
Identities = 14/22 (63%), Positives = 14/22 (63%)
Frame = -1 / -1
Query: 1428 IPGQLLRPTPKGRYLKSLPLAS 1363
IPGQ LRP P G K LPL S
Sbjct: 312 IPGQDLRPDPNGINSKLLPLKS 247
>gb|CO514319.1|CO514319 s13dSG84A1000069_157300 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 564
Score = 61.1 bits (127), Expect = 4e-010
Identities = 27/99 (27%), Positives = 47/99 (47%)
Frame = +2 / +3
Query: 227 GDDLNHRNLAALSRKFGDVFLLRMGQRNLVVVSSPPLAREVLHTQGVEFGSRTRNVVFDI 406
G H L L+ K+G +F LR+G +VVSS + ++ F SR +++ F+I
Sbjct: 219 GSQPPHVTLGNLADKYGPIFTLRLGVHKTLVVSSYEMVKQCFTVNDKAFASRPKSIAFEI 398
Query: 407 FTDKGQDMVFTVYGDHWRKMRRIMTVPFFTNKVVQQYRH 523
+ YG +WR +R+I T + + + +H
Sbjct: 399 MGYNSSMFGLSPYGSYWRTVRKIATGHVLSAQRIDMLKH 515
>gb|CO515393.1|CO515393 s13dSG49G0500040_417845 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 573
Score = 33.1 bits (66), Expect(2) = 7e-006
Identities = 17/61 (27%), Positives = 28/61 (45%)
Frame = +2 / +2
Query: 1127 DTHNLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWYLANNPDS 1306
+ L YL+A + ETLRL ++P H+ D G +PA S + + + +
Sbjct: 296 EVDRLVYLKAALSETLRLYPSVPQDSKHVVNDDVLPNGTFVPAGSMVTYSIYSVGRMKFI 475
Query: 1307 W 1309
W
Sbjct: 476 W 478
Score = 31.8 bits (63), Expect(2) = 7e-006
Identities = 14/55 (25%), Positives = 32/55 (58%)
Frame = +2 / +2
Query: 935 QQKGEINEDNVLFIVENINVAAIETTLWSIEWAVAELVNHPEIQQKLRQELDTVL 1099
++K + +E+ + + N +A +T+ ++ W + +PE+++K+ EL TVL
Sbjct: 74 KKKEKYSEEFLQHVALNFILAGRDTSSVALSWFFWLCMKNPEVEEKILVELCTVL 238
>gb|CO513758.1|CO513758 s13dSG15G1200088_140298 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 473
Score = 39.1 bits (79), Expect = 0.002
Identities = 15/42 (35%), Positives = 19/42 (45%)
Frame = +2 / +1
Query: 1361 VEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFE 1486
+ GN+F +PFG GRR CP L+ NFE
Sbjct: 73 INYKGNNFELIPFGAGRRICPGLPLAHRTMHLMVASLLHNFE 198
Score = 29.5 bits (58), Expect = 1.3
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = -1 / -1
Query: 1425 PGQLLRPTPKGRYLKSLPL 1369
PGQ+L P PKG K LPL
Sbjct: 137 PGQILLPAPKGISSKLLPL 81
>gb|CO513530.1|CO513530 s13dSG12E0600039_139842 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 499
Score = 36.8 bits (74), Expect = 0.008
Identities = 15/42 (35%), Positives = 18/42 (42%)
Frame = +2 / +3
Query: 1373 GNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPP 1498
G F LPFG GRR CP ++Q F+L P
Sbjct: 6 GQHFTLLPFGSGRRMCPGVNLATAGMATLLSSIIQCFDLQVP 131
Score = 27.6 bits (54), Expect = 4.8
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -1 / -1
Query: 1437 ARMIPGQLLRPTPKGRYLKSLP 1372
A++ PG +L P P GR +K P
Sbjct: 70 AKLTPGHILLPDPNGRSVKC*P 5
>gb|CO513716.1|CO513716 s13dSG15C0700050_140214 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 300
Score = 36.3 bits (73), Expect = 0.012
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +2 / +2
Query: 1361 VEANGNDFRYLPFGVGRRSC 1420
V+ GNDF +PFG GRR C
Sbjct: 125 VDVKGNDFEVIPFGAGRRIC 184
Score = 31.8 bits (63), Expect = 0.28
Identities = 13/25 (52%), Positives = 14/25 (56%)
Frame = -1 / -1
Query: 1434 RMIPGQLLRPTPKGRYLKSLPLAST 1360
R P +LRP PKG KS P ST
Sbjct: 198 RFKPAHILRPAPKGITSKSFPFTST 124
>gb|CO517155.1|CO517155 s13dSG33C0300022_474394 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 571
Score = 35.9 bits (72), Expect = 0.016
Identities = 11/17 (64%), Positives = 15/17 (88%)
Frame = +2 / -1
Query: 1373 GNDFRYLPFGVGRRSCP 1423
G++F ++PFG GRRSCP
Sbjct: 541 GSNFEFIPFGSGRRSCP 491
Score = 30.4 bits (60), Expect = 0.71
Identities = 14/22 (63%), Positives = 14/22 (63%)
Frame = -1 / +3
Query: 1428 IPGQLLRPTPKGRYLKSLPLAS 1363
IPGQ LRP P G K LPL S
Sbjct: 486 IPGQDLRPDPNGINSKLLPLKS 551
>gb|CO514053.1|CO514053 s13dSG72G0600040_156768 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 552
Score = 24.0 bits (46), Expect(3) = 0.46
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = -1 / -2
Query: 264 DSAARLRWLRSSPTCSQFPK 205
+S RW+ SP+ Q+PK
Sbjct: 485 NSYTNSRWIPLSPSTEQYPK 426
Score = 23.1 bits (44), Expect(3) = 0.46
Identities = 7/16 (43%), Positives = 7/16 (43%)
Frame = -3 / -1
Query: 82 HGDGAEMEGGLACCPF 35
H G G CCPF
Sbjct: 153 HTPGRRYRAGRVCCPF 106
Score = 21.7 bits (41), Expect(3) = 0.46
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = -1 / -2
Query: 162 PRILDTAMATMTDAKRPT 109
PR+L MAT+T + P+
Sbjct: 350 PRVLSLLMATITGSPCPS 297
>gb|CO514481.1|CO514481 s13dSG43C0400024_327622 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 599
Score = 28.6 bits (56), Expect = 2.5
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = +1 / -1
Query: 1519 HHREGRPVQSPHLEAFHHRVQAKNALRAAH 1608
H R R V PHL+ FH + +N H
Sbjct: 566 HFRAIRKVAPPHLDCFHQGLHPRNKSLVLH 477
>gb|CO514097.1|CO514097 s13dSG75C0500034_156856 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 516
Score = 28.1 bits (55), Expect = 3.5
Identities = 11/35 (31%), Positives = 21/35 (60%)
Frame = +2 / -1
Query: 731 QSFEYNYGDFIPILRPFLRGYLRVCKEVKETRLKL 835
+S E N+G+ I L+P L+ Y+ ++ R+K+
Sbjct: 474 ESKERNHGNAITHLKPLLKNYVVRLNQISPFRIKI 370
>gb|CO512204.1|CO512204 s13dSG03C0400022_113922 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 593
Score = 27.6 bits (54), Expect = 4.8
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -2 / +1
Query: 1607 WAALKAFLACTRWWNASRC 1551
W++ K TRWWN+S C
Sbjct: 403 WSSCKCR*RKTRWWNSS*C 459
>gb|CO513310.1|CO513310 s13dSG37E0600039_129784 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 554
Score = 27.6 bits (54), Expect = 4.8
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -2 / +1
Query: 1607 WAALKAFLACTRWWNASRC 1551
W++ K TRWWN+S C
Sbjct: 403 WSSCKCR*RKTRWWNSS*C 459
>gb|CO514948.1|CO514948 s13dSG63D0500046_397401 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 494
Score = 27.6 bits (54), Expect = 4.8
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -2 / +2
Query: 1607 WAALKAFLACTRWWNASRC 1551
W++ K TRWWN+S C
Sbjct: 374 WSSCKCR*RKTRWWNSS*C 430
>gb|CO515338.1|CO515338 s13dSG48H0200028_417735 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 555
Score = 27.6 bits (54), Expect = 4.8
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -2 / +1
Query: 1607 WAALKAFLACTRWWNASRC 1551
W++ K TRWWN+S C
Sbjct: 403 WSSCKCR*RKTRWWNSS*C 459
>gb|CO517126.1|CO517126 s13dSG29G0600052_468798 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 496
Score = 27.6 bits (54), Expect = 4.8
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -2 / +2
Query: 1607 WAALKAFLACTRWWNASRC 1551
W++ K TRWWN+S C
Sbjct: 404 WSSCKCR*RKTRWWNSS*C 460
>gb|CO512424.1|CO512424 s13dSG80G0300020_114362 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 556
Score = 26.7 bits (52), Expect = 9.1
Identities = 10/21 (47%), Positives = 11/21 (52%)
Frame = +1 / +2
Query: 94 RGEAPGRPLGVRHGRHRGVQD 156
R A G P GV H HR + D
Sbjct: 98 RRSAVGNPKGVHHRLHRSISD 160
>gb|CO512747.1|CO512747 s13dSG88B0900073_121420 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 580
Score = 26.7 bits (52), Expect = 9.1
Identities = 16/40 (40%), Positives = 20/40 (50%)
Frame = -3 / -1
Query: 1717 AFFLPVLLLSTNDTA*LSPNNMVMEXNIRLLRMEPTCGLL 1598
A L +LLLST L P + IR L ++PT LL
Sbjct: 298 ALQLILLLLSTQTIFGLQP*RPCQKTKIRCLSLDPTHDLL 179
>gb|CO516408.1|CO516408 s13dSG27D0500043_446144 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 466
Score = 26.7 bits (52), Expect = 9.1
Identities = 9/19 (47%), Positives = 11/19 (57%)
Frame = -2 / +3
Query: 1607 WAALKAFLACTRWWNASRC 1551
W+ K TRWWN+S C
Sbjct: 402 WSPCKCR*RKTRWWNSS*C 458
>gb|CO517095.1|CO517095 s13dSG29C1200098_468736 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 490
Score = 26.7 bits (52), Expect = 9.1
Identities = 16/38 (42%), Positives = 22/38 (57%)
Frame = -1 / +2
Query: 999 AATLMFSTMKSTLSSLISPFCCASSM*SMAHLRPLLSV 886
++TL F + + LISP AS + S AHL P+ SV
Sbjct: 47 SSTLGFQAAVARAARLISPPHQASVVFS*AHLSPVTSV 160
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: May 2, 2006 2:40 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,286,687
Number of Sequences: 7669
Number of extensions: 106635
Number of successful extensions: 1124
Number of sequences better than 10.0: 33
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 503855996
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)