TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5705280.3.1
(980 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO513352.1|CO513352 s13dSG38A0400021_129868 Glandular tr... 35 0.017
gb|DQ122815.1| Medicago sativa clone B13 mRNA sequence 31 0.28
gb|CO513510.1|CO513510 s13dSG12C0300018_139802 Glandular tr... 29 0.77
gb|CO511951.1|CO511951 s13dSG05H1200104_103802 Glandular tr... 29 0.99
gb|AJ888490.1|AJ888490 AJ888490 Medicago sativa subsp. x va... 28 1.4
gb|CO512841.1|CO512841 s13dSG20D0400030_121608 Glandular tr... 28 1.9
gb|CO515697.1|CO515697 s13dSG60G0300024_419543 Glandular tr... 23 2.6
gb|CO515169.1|CO515169 s13dSG62B1200101_399203 Glandular tr... 28 2.7
gb|CO512493.1|CO512493 s13dSG100F100007_114500 Glandular tr... 27 3.5
gb|CO514893.1|CO514893 s13dSG56F0300031_328446 Glandular tr... 27 3.5
gb|CO515579.1|CO515579 s13dSG47A1000081_418217 Glandular tr... 27 3.5
gb|CO517319.1|CO517319 s13dSG45F0800075_486234 Glandular tr... 27 3.5
gb|AJ248326.1|AJ248326 AJ248326 Medicago sativa subsp. x va... 27 3.8
gb|CO513464.1|CO513464 s13dSG10E0500035_130092 Glandular tr... 27 3.8
gb|CO511782.1|CO511782 s13dSG02A0400033_103464 Glandular tr... 27 4.8
gb|CO514474.1|CO514474 s13dSG43B0500043_327608 Glandular tr... 27 4.8
gb|CO516527.1|CO516527 s13dSG57B0500042_446382 Glandular tr... 27 4.8
gb|CO516569.1|CO516569 s13dSG57F0900077_446466 Glandular tr... 27 4.8
gb|U41472.1|MSU41472 Medicago sativa pectate lyase homolog ... 27 5.2
dbj|AB028150.1| Medicago sativa MsU9 mRNA for uricase (Nod-... 27 5.2
gb|AJ248336.1|AJ248336 AJ248336 Medicago sativa subsp. x va... 26 6.7
gb|CO512261.1|CO512261 s13dSG03H0500044_114036 Glandular tr... 26 6.7
gb|CO512943.1|CO512943 s13dSG86F0800063_121812 Glandular tr... 26 6.7
emb|AJ319667.1|MSA319667 Medicago sativa subsp. falcata mRN... 23 8.1
gb|CO512668.1|CO512668 s13dSG19B1000077_121262 Glandular tr... 26 9.2
gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular tr... 26 9.2
gb|CO513908.1|CO513908 s13dSG71A0600037_156478 Glandular tr... 26 9.2
gb|CO516165.1|CO516165 s13dSG67B0900077_445658 Glandular tr... 26 9.2
>gb|CO513352.1|CO513352 s13dSG38A0400021_129868 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 543
Score = 34.8 bits (70), Expect = 0.017
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +1 / +1
Query: 787 GGFQCTCPAGMSGDGLK 837
GG +C CPAG GDG+K
Sbjct: 4 GGVRCECPAGFEGDGVK 54
Score = 27.6 bits (54), Expect(2) = 0.052
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = +1 / +1
Query: 655 GIGYRCNCSSGFEGN 699
G G RC C +GFEG+
Sbjct: 1 GGGVRCECPAGFEGD 45
Score = 23.0 bits (44), Expect(2) = 0.052
Identities = 7/17 (41%), Positives = 9/17 (52%)
Frame = +1 / +1
Query: 712 GPNGCTDINECITRNPC 762
G C DI+EC + C
Sbjct: 46 GVKNCADIDECKEKKAC 96
>gb|DQ122815.1| Medicago sativa clone B13 mRNA sequence
Length = 1174
Score = 30.7 bits (61), Expect = 0.28
Identities = 10/30 (33%), Positives = 20/30 (66%)
Frame = +3 / -1
Query: 213 ILRHKEQVHSSGL*RGGHAFEWQHGV*RWL 302
++ ++++ S L*RG ++W+ G+ RWL
Sbjct: 145 VVSREKELESLDL*RGNWNWDWRRGIRRWL 56
>gb|CO513510.1|CO513510 s13dSG12C0300018_139802 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 530
Score = 29.4 bits (58), Expect = 0.77
Identities = 8/28 (28%), Positives = 16/28 (57%)
Frame = +1 / -3
Query: 448 DYWPCGQAFVVEQDSYVFSALDLNNTND 531
D+W C Q + + Q+ ++ ++ LN D
Sbjct: 306 DHWGCSQQYAIWQNGHLAGSMQLNERQD 223
>gb|CO511951.1|CO511951 s13dSG05H1200104_103802 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 585
Score = 28.9 bits (57), Expect = 0.99
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = -1 / -3
Query: 761 HGFLVMHSLISVHPFGPCRYGFPSNPL 681
HG+L +H S+ PC + P +PL
Sbjct: 112 HGYLRLHPAQSIDLQSPCHHDHPHHPL 32
>gb|AJ888490.1|AJ888490 AJ888490 Medicago sativa subsp. x varia 4-day-old Medicago sativa
subsp. x varia cDNA, mRNA sequence
Length = 434
Score = 28.5 bits (56), Expect = 1.4
Identities = 12/38 (31%), Positives = 18/38 (47%)
Frame = -2 / +3
Query: 526 LCCSDQELKTHMNPAPRRRPDHMASNRNRCRCWLY*YY 413
+CC + T+ A RRR +H A + C+ YY
Sbjct: 33 ICCQVIDFSTNPKDAERRRTNHRALHS*EVFCYKQAYY 146
>gb|CO512841.1|CO512841 s13dSG20D0400030_121608 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 397
Score = 28.0 bits (55), Expect = 1.9
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = -2 / -2
Query: 364 SKPPQSMSRR*CYFH*SKRKHSHRYTPCCHSKAWP 260
S+ P + +RR CY+ + H + TPC + + P
Sbjct: 342 SEAPNN*NRRMCYYFVIVKVHHNSSTPCKYQEVRP 238
>gb|CO515697.1|CO515697 s13dSG60G0300024_419543 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 506
Score = 23.4 bits (45), Expect(2) = 2.6
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = -1 / -2
Query: 191 LTLEFAVPPMH 159
LTLE A+PP+H
Sbjct: 505 LTLEAALPPLH 473
Score = 23.0 bits (44), Expect(2) = 2.6
Identities = 6/11 (54%), Positives = 8/11 (72%)
Frame = -2 / -1
Query: 112 TRCQESQACCF 80
+RC E + CCF
Sbjct: 77 SRCTEERVCCF 45
>gb|CO515169.1|CO515169 s13dSG62B1200101_399203 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 500
Score = 27.6 bits (54), Expect = 2.7
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +1 / +1
Query: 571 VNGSSCEEAKLSGSYACAENAYCYNSS 651
++GS+ E + G C AYCYN+S
Sbjct: 271 ISGSAGEAIRSEGIC*CFH*AYCYNAS 351
>gb|CO512493.1|CO512493 s13dSG100F100007_114500 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 526
Score = 27.1 bits (53), Expect = 3.5
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = -1 / -3
Query: 296 PLYPVLPFKSMATTLQP 246
P +PV+ FKSM+ LQP
Sbjct: 338 PRHPVINFKSMSCRLQP 288
>gb|CO514893.1|CO514893 s13dSG56F0300031_328446 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 620
Score = 27.1 bits (53), Expect = 3.5
Identities = 10/31 (32%), Positives = 12/31 (38%)
Frame = +2 / +1
Query: 26 CPVDLKLCVDTVVRCCPSETACLGFLTSRCL 118
CP++ C D CCP E CL
Sbjct: 280 CPLESATCCDDHYSCCPHEYPVCDTRAGLCL 372
>gb|CO515579.1|CO515579 s13dSG47A1000081_418217 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 322
Score = 27.1 bits (53), Expect = 3.5
Identities = 9/25 (36%), Positives = 11/25 (44%)
Frame = -2 / -1
Query: 121 HQATRCQESQACCFRWTATDHCVHT 47
H T+ C WT T C+HT
Sbjct: 298 HHKTKVMHVIKACHLWTLT*KCIHT 224
>gb|CO517319.1|CO517319 s13dSG45F0800075_486234 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 652
Score = 27.1 bits (53), Expect = 3.5
Identities = 9/19 (47%), Positives = 11/19 (57%)
Frame = -2 / -3
Query: 733 YLCIHLALAGMGSPRIHWS 677
Y C H +L G P I+WS
Sbjct: 182 YQCCHFSLEGHNQPFINWS 126
>gb|AJ248326.1|AJ248326 AJ248326 Medicago sativa subsp. x varia young root nodule Medicago
sativa subsp. x varia cDNA clone MsNod374b, mRNA
sequence
Length = 475
Score = 27.1 bits (53), Expect = 3.8
Identities = 12/42 (28%), Positives = 19/42 (45%)
Frame = +1 / +3
Query: 259 VAMLLNGSTGYSGGCASFCSSGNNIIDGSCSGVACCQAPVPK 384
+++LL + GY +SF D C ++C VPK
Sbjct: 51 ISLLLVVTKGYREPFSSFIEGPTCKEDADCPSISCVNPQVPK 176
>gb|CO513464.1|CO513464 s13dSG10E0500035_130092 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 587
Score = 27.1 bits (53), Expect = 3.8
Identities = 12/53 (22%), Positives = 20/53 (37%)
Frame = +1 / +2
Query: 580 SSCEEAKLSGSYACAENAYCYNSSNGIGYRCNCSSGFEGNPYLQGPNGCTDIN 738
+S + C+E+ YC N++ G C + G P D+N
Sbjct: 131 ASAQNCGCEEGLCCSEHGYCGNTAAYCGTGCQQGPCYAGQISPSTPGPSNDVN 289
>gb|CO511782.1|CO511782 s13dSG02A0400033_103464 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 536
Score = 26.6 bits (52), Expect = 4.8
Identities = 8/19 (42%), Positives = 10/19 (52%)
Frame = +2 / +2
Query: 26 CPVDLKLCVDTVVRCCPSE 82
CP++ C D CCP E
Sbjct: 449 CPLESATCCDDRYSCCPHE 505
>gb|CO514474.1|CO514474 s13dSG43B0500043_327608 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 604
Score = 26.6 bits (52), Expect = 4.8
Identities = 13/51 (25%), Positives = 22/51 (43%)
Frame = -3 / +3
Query: 174 RPSYALLRTSTSQYAHKTIKQRDVKNPKHAVSDGQQRTTVSTHNFKSTGQG 22
+P Y +++ S + A + D+ +PK S R T K G+G
Sbjct: 126 KPRYPIVKGSDTSTAESGSEAEDIASPKAMKSYSHLRLTPVREEAKIAGKG 278
>gb|CO516527.1|CO516527 s13dSG57B0500042_446382 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 504
Score = 26.6 bits (52), Expect = 4.8
Identities = 12/38 (31%), Positives = 18/38 (47%)
Frame = -1 / -3
Query: 326 FPLEQKEAQPPLYPVLPFKSMATTLQPRAVNLFLVSEN 213
FP E+K + K M T P+++ LFL S +
Sbjct: 142 FPFEKKTGSAATADFVESKLMFTKFPPKSLTLFLYSRS 29
>gb|CO516569.1|CO516569 s13dSG57F0900077_446466 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 493
Score = 26.6 bits (52), Expect = 4.8
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = -2 / +1
Query: 802 CTGNHLLCLSTYWCTDFW*CIH 737
C G LLC S +WC+ C H
Sbjct: 325 CYGFGLLCWSHFWCSFQSCCYH 390
>gb|U41472.1|MSU41472 Medicago sativa pectate lyase homolog gene, complete cds
Length = 3824
Score = 26.6 bits (52), Expect = 5.2
Identities = 11/28 (39%), Positives = 14/28 (50%)
Frame = +1 / -1
Query: 598 KLSGSYACAENAYCYNSSNGIGYRCNCS 681
KL+ + A N+Y N N CNCS
Sbjct: 1442 KLTNTTLKAWNSYGQNQQNKANLHCNCS 1359
>dbj|AB028150.1| Medicago sativa MsU9 mRNA for uricase (Nod-35), complete cds
Length = 1303
Score = 26.6 bits (52), Expect = 5.2
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +1 / -2
Query: 382 KGLKKLDLEFSNINITNSGIDYDYW 456
KGL KL F N+N +DYW
Sbjct: 1185 KGLIKLYWNF*NLNYLQPNNXFDYW 1111
>gb|AJ248336.1|AJ248336 AJ248336 Medicago sativa subsp. x varia young root nodule Medicago
sativa subsp. x varia cDNA clone MsNod658, mRNA sequence
Length = 1004
Score = 26.2 bits (51), Expect = 6.7
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = -2 / +1
Query: 625 LRRRMIRTVSPLHSCCRSQTTRAQHVGIEVLC 530
+ R+ RTV LHS + + Q +GI LC
Sbjct: 202 MSRKECRTVRRLHSLVKPMKHQTQLLGILYLC 297
>gb|CO512261.1|CO512261 s13dSG03H0500044_114036 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 524
Score = 26.2 bits (51), Expect = 6.7
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -2 / -3
Query: 532 CRLCCSDQ 509
CRLCCSDQ
Sbjct: 174 CRLCCSDQ 151
>gb|CO512943.1|CO512943 s13dSG86F0800063_121812 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 585
Score = 26.2 bits (51), Expect = 6.7
Identities = 7/25 (28%), Positives = 11/25 (44%)
Frame = -2 / -3
Query: 124 DHQATRCQESQACCFRWTATDHCVH 50
D R + ++CC+ W C H
Sbjct: 319 DRHRWRRDDRRSCCYHWNRDRDCFH 245
>emb|AJ319667.1|MSA319667 Medicago sativa subsp. falcata mRNA for beta-tubulin
Length = 1281
Score = 23.4 bits (45), Expect(2) = 8.1
Identities = 6/7 (85%), Positives = 7/7 (100%)
Frame = +3 / +3
Query: 45 CVWTQWS 65
CVWT+WS
Sbjct: 201 CVWTKWS 221
Score = 22.5 bits (43), Expect(2) = 8.1
Identities = 7/14 (50%), Positives = 10/14 (71%)
Frame = +1 / +1
Query: 325 NNIIDGSCSGVACC 366
N++I + SGV CC
Sbjct: 607 NHLISTTMSGVTCC 648
>gb|CO512668.1|CO512668 s13dSG19B1000077_121262 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 516
Score = 25.7 bits (50), Expect = 9.2
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -2 / +3
Query: 487 PAPRRRPDHMASNRNRCR 434
P RRRP+HM+S+ R +
Sbjct: 102 PLHRRRPEHMSSSSPRIK 155
>gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 602
Score = 25.7 bits (50), Expect = 9.2
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = -2 / -1
Query: 409 TLSPASLALWEPALGS 362
+L+P+SL W P LGS
Sbjct: 215 SLAPSSLTKWSPFLGS 168
>gb|CO513908.1|CO513908 s13dSG71A0600037_156478 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 397
Score = 25.7 bits (50), Expect = 9.2
Identities = 12/37 (32%), Positives = 16/37 (43%)
Frame = -2 / +3
Query: 523 CCSDQELKTHMNPAPRRRPDHMASNRNRCRCWLY*YY 413
CC + T A RRR +H A + C+ YY
Sbjct: 51 CCQVIDFSTIPKDAERRRTNHRALHS*EVFCYKQAYY 161
>gb|CO516165.1|CO516165 s13dSG67B0900077_445658 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 515
Score = 25.7 bits (50), Expect = 9.2
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -2 / +2
Query: 487 PAPRRRPDHMASNRNRCR 434
P RRRP+HM+S+ R +
Sbjct: 47 PLHRRRPEHMSSSSPRIK 100
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: May 2, 2006 2:40 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.316 0.133 0.426
Matrix: BLOSUM62
Number of Hits to DB: 9,722,593
Number of Sequences: 7669
Number of extensions: 155730
Number of successful extensions: 1720
Number of sequences better than 10.0: 32
length of database: 1,248,568
effective HSP length: 40
effective length of database: 941,808
effective search space used: 269357088
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.6 bits)