TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4548790.2.1
(1044 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO513205.1|CO513205 s13dSG24B0800061_129574 Glandular tr... 163 7e-067
gb|CO514666.1|CO514666 s13dSG46E1100087_327992 Glandular tr... 139 2e-062
gb|CO514640.1|CO514640 s13dSG46C0600050_327940 Glandular tr... 129 8e-062
gb|CO515743.1|CO515743 s13dSG77C1100086_419635 Glandular tr... 102 3e-049
gb|CO515120.1|CO515120 s13dSG40E0200019_399105 Glandular tr... 133 4e-032
>gb|CO513205.1|CO513205 s13dSG24B0800061_129574 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 592
Score = 163 bits (351), Expect(3) = 7e-067
Identities = 61/79 (77%), Positives = 71/79 (89%)
Frame = +2 / +2
Query: 236 SILVTATNFCPPNYALPSDDGGWCNPPRPHFDLAMPMFLHIAEYRAGIVPVSYRRVPCRK 415
SILVTATNFCPPN ALP+++GGWC+PP HFDLA P+FL IA+Y+AGIVPVSYRRVPCR+
Sbjct: 356 SILVTATNFCPPNNALPNNNGGWCSPPLQHFDLAQPVFLRIAQYKAGIVPVSYRRVPCRR 535
Query: 416 SGGVRFTINGFRYFNLVLI 472
GG+RFTING YFNLVL+
Sbjct: 536 RGGIRFTINGHSYFNLVLV 592
Score = 98.2 bits (208), Expect(2) = 4e-030
Identities = 47/77 (61%), Positives = 53/77 (68%)
Frame = -1 / -2
Query: 465 TRLKYRKPLIVNRTPPDLRHGTRR*ETGTMPARYSAMWRNMGMARSKCGRGGLHQPPSSE 286
T+LK PLIVN PP L GT *ETGT+PA Y A+ R G ARSKC +GGLH PP
Sbjct: 585 TKLK*ECPLIVNLIPPLLLQGTLL*ETGTIPALY*AILRKTG*ARSKCCKGGLHHPPLLL 406
Query: 285 GSA*LGGQKLVAVTRME 235
G A GGQKLVAVT++E
Sbjct: 405 GKALFGGQKLVAVTKIE 355
Score = 65.7 bits (137), Expect(3) = 7e-067
Identities = 25/36 (69%), Positives = 27/36 (75%)
Frame = +2 / +2
Query: 104 SQGYGVNNAALSTALFDEGLRCGACFEIRCEEQPGW 211
SQGYG N AALSTALF+ GL CG+CFEIRC W
Sbjct: 236 SQGYGTNTAALSTALFNSGLSCGSCFEIRCAGDRKW 343
Score = 58.8 bits (122), Expect(3) = 7e-067
Identities = 23/28 (82%), Positives = 23/28 (82%)
Frame = +1 / +2
Query: 28 AHATFYGGSDAFGFMGGACGYGNL*QPG 111
AHATFYGGSDA G MGGACGYGNL G
Sbjct: 161 AHATFYGGSDASGTMGGACGYGNLYSQG 244
Score = 50.6 bits (104), Expect(2) = 4e-030
Identities = 20/28 (71%), Positives = 21/28 (75%)
Frame = -2 / -2
Query: 110 PGCHRLPYPQAPPIKPKASLPP*NVACA 27
P +R PYP APPI P ASLPP* VACA
Sbjct: 243 P*LYRFPYPHAPPIVPDASLPP*KVACA 160
>gb|CO514666.1|CO514666 s13dSG46E1100087_327992 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 542
Score = 139 bits (299), Expect(3) = 2e-062
Identities = 50/59 (84%), Positives = 54/59 (91%)
Frame = +2 / +1
Query: 212 RWCRPGRPSILVTATNFCPPNYALPSDDGGWCNPPRPHFDLAMPMFLHIAEYRAGIVPV 388
RWC PG PSIL+TATNFCPPN+A PSD+GGWCNPPRPH DLAMPMFL IA+YRAGIVPV
Sbjct: 364 RWCNPGNPSILITATNFCPPNFAQPSDNGGWCNPPRPHSDLAMPMFLKIAQYRAGIVPV 540
Score = 87.2 bits (184), Expect(2) = 6e-031
Identities = 37/59 (62%), Positives = 45/59 (76%)
Frame = -1 / -1
Query: 387 TGTMPARYSAMWRNMGMARSKCGRGGLHQPPSSEGSA*LGGQKLVAVTRMEGLPGRHHR 211
TGT+PARY A+ RN+G+ARS+ G GGLHQPP S G A GGQK VAV +++G PG H R
Sbjct: 539 TGTIPARY*AILRNIGIARSE*GLGGLHQPPLSLG*AKFGGQKFVAVIKIDGFPGLHQR 363
Score = 73.9 bits (155), Expect(3) = 2e-062
Identities = 26/36 (72%), Positives = 31/36 (86%)
Frame = +2 / +1
Query: 104 SQGYGVNNAALSTALFDEGLRCGACFEIRCEEQPGW 211
SQGYGVN AALSTALF+ GL CGACFE++C++ P W
Sbjct: 262 SQGYGVNTAALSTALFNNGLSCGACFELKCDQDPRW 369
Score = 62.0 bits (129), Expect(2) = 6e-031
Identities = 27/43 (62%), Positives = 29/43 (67%)
Frame = -1 / -1
Query: 204 GCSSHLISKHAPQRSPSSNSAVLSAALFTPYPWLSQVAVPAGP 76
G SHL SKHAPQ P NSAVLSAA+FTP PWL + P P
Sbjct: 362 GS*SHLSSKHAPQLKPLLNSAVLSAAVFTP*PWLYRFPYPQAP 234
Score = 59.7 bits (124), Expect(3) = 2e-062
Identities = 24/32 (75%), Positives = 24/32 (75%)
Frame = +1 / +1
Query: 16 PRRRAHATFYGGSDAFGFMGGACGYGNL*QPG 111
P AHATFYGGSDA G MGGACGYGNL G
Sbjct: 175 PWTSAHATFYGGSDASGTMGGACGYGNLYSQG 270
>gb|CO514640.1|CO514640 s13dSG46C0600050_327940 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 536
Score = 129 bits (277), Expect(4) = 8e-062
Identities = 47/65 (72%), Positives = 56/65 (86%)
Frame = +2 / +3
Query: 296 GGWCNPPRPHFDLAMPMFLHIAEYRAGIVPVSYRRVPCRKSGGVRFTINGFRYFNLVLIT 475
GGWC+PP HFDL+ P+F HIA+YRAGIVPV+YRRV C++ GG+RFTING YFNLVL+T
Sbjct: 342 GGWCDPPNHHFDLSQPIFQHIAQYRAGIVPVAYRRVRCKRRGGIRFTINGHSYFNLVLVT 521
Query: 476 NVAGA 490
NV GA
Sbjct: 522 NVGGA 536
Score = 70.3 bits (147), Expect(4) = 1e-034
Identities = 36/68 (52%), Positives = 41/68 (60%)
Frame = -1 / -2
Query: 489 APATFVISTRLKYRKPLIVNRTPPDLRHGTRR*ETGTMPARYSAMWRNMGMARSKCGRGG 310
AP TFV T+LK PL+VN PP L H T * TGT+PA Y AM +G RSK GG
Sbjct: 535 APPTFVTRTKLK*ECPLMVNLIPPLLLHLTLL*ATGTIPALY*AMC*KIG*ERSKW*LGG 356
Query: 309 LHQPPSSE 286
HQPP +
Sbjct: 355 SHQPPGGQ 332
Score = 68.9 bits (144), Expect(4) = 8e-062
Identities = 25/36 (69%), Positives = 28/36 (77%)
Frame = +2 / +3
Query: 104 SQGYGVNNAALSTALFDEGLRCGACFEIRCEEQPGW 211
SQGYG N AALSTALF+ GL CGAC+EI+C P W
Sbjct: 186 SQGYGTNTAALSTALFNNGLSCGACYEIKCVNDPQW 293
Score = 58.8 bits (122), Expect(4) = 8e-062
Identities = 23/28 (82%), Positives = 23/28 (82%)
Frame = +1 / +3
Query: 28 AHATFYGGSDAFGFMGGACGYGNL*QPG 111
AHATFYGGSDA G MGGACGYGNL G
Sbjct: 111 AHATFYGGSDASGTMGGACGYGNLYSQG 194
Score = 58.3 bits (121), Expect(4) = 1e-034
Identities = 25/45 (55%), Positives = 30/45 (66%)
Frame = -1 / -2
Query: 210 HPGCSSHLISKHAPQRSPSSNSAVLSAALFTPYPWLSQVAVPAGP 76
H G +HLIS HAPQ +P NSAVL+AA+F P PWL + P P
Sbjct: 292 HCGSFTHLIS*HAPQLNPLLNSAVLNAAVFVP*PWL*RFPYPQAP 158
Score = 46.0 bits (94), Expect(4) = 1e-034
Identities = 18/22 (81%), Positives = 19/22 (86%)
Frame = -2 / -2
Query: 98 RLPYPQAPPIKPKASLPP*NVA 33
R PYPQAPPI P+ASLPP* VA
Sbjct: 181 RFPYPQAPPIVPEASLPP*KVA 116
Score = 31.8 bits (63), Expect(4) = 8e-062
Identities = 11/12 (91%), Positives = 12/12 (100%)
Frame = +2 / +3
Query: 236 SILVTATNFCPP 271
SI+VTATNFCPP
Sbjct: 306 SIIVTATNFCPP 341
Score = 22.6 bits (43), Expect(4) = 1e-034
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = -1 / -2
Query: 270 GGQKLVAVT 244
GGQKLVAVT
Sbjct: 340 GGQKLVAVT 314
>gb|CO515743.1|CO515743 s13dSG77C1100086_419635 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 538
Score = 102 bits (217), Expect(4) = 3e-049
Identities = 37/50 (74%), Positives = 44/50 (88%)
Frame = +2 / +1
Query: 236 SILVTATNFCPPNYALPSDDGGWCNPPRPHFDLAMPMFLHIAEYRAGIVP 385
SI+VTATNFCPPN +LP+++GGWCNPP HFD+A P +L IAEYRAGIVP
Sbjct: 388 SIIVTATNFCPPNPSLPNNNGGWCNPPLQHFDMAEPAYLQIAEYRAGIVP 537
Score = 69.3 bits (145), Expect(4) = 3e-049
Identities = 24/36 (66%), Positives = 29/36 (80%)
Frame = +2 / +1
Query: 104 SQGYGVNNAALSTALFDEGLRCGACFEIRCEEQPGW 211
SQGYG N AALSTALF+ GL CG+C+E+RC + P W
Sbjct: 268 SQGYGTNTAALSTALFNSGLSCGSCYEMRCNDDPRW 375
Score = 64.3 bits (134), Expect(2) = 4e-022
Identities = 30/50 (60%), Positives = 34/50 (68%)
Frame = -1 / -3
Query: 384 GTMPARYSAMWRNMGMARSKCGRGGLHQPPSSEGSA*LGGQKLVAVTRME 235
GT+PA YSA+ R G A SKC +GGLHQPP G GGQK VAVT +E
Sbjct: 536 GTIPALYSAI*R*AGSAISKCCKGGLHQPPLLLGKDGFGGQKFVAVTIIE 387
Score = 57.0 bits (118), Expect(4) = 3e-049
Identities = 22/28 (78%), Positives = 22/28 (78%)
Frame = +1 / +1
Query: 28 AHATFYGGSDAFGFMGGACGYGNL*QPG 111
AHATFYGG DA G MGGACGYGNL G
Sbjct: 193 AHATFYGGGDASGTMGGACGYGNLYSQG 276
Score = 55.1 bits (114), Expect(2) = 4e-022
Identities = 24/45 (53%), Positives = 26/45 (57%)
Frame = -1 / -3
Query: 210 HPGCSSHLISKHAPQRSPSSNSAVLSAALFTPYPWLSQVAVPAGP 76
H G S HLIS PQ P N AVLSAA+ PYPWL + P P
Sbjct: 374 HLGSSLHLIS*QDPQLKPLLNKAVLSAAVLVPYPWLYKFPYPHAP 240
Score = 21.7 bits (41), Expect(4) = 3e-049
Identities = 6/13 (46%), Positives = 8/13 (61%)
Frame = +2 / +1
Query: 212 RWCRPGRPSILVT 250
RWC+PG + T
Sbjct: 370 RWCKPGSIIVTAT 408
>gb|CO515120.1|CO515120 s13dSG40E0200019_399105 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 314
Score = 133 bits (285), Expect = 4e-032
Identities = 52/90 (57%), Positives = 64/90 (71%)
Frame = +2 / +3
Query: 431 FTINGFRYFNLVLITNVAGAGDVVRASVKAASTGWLPMSRNWGQNWQSNAILVGQALSFR 610
FTING YFNLVL+TNV GAGDV ++K + T W MSRNWGQNWQSN+ L GQ+LS
Sbjct: 6 FTINGHSYFNLVLVTNVGGAGDVHSVAIKGSRTRWQAMSRNWGQNWQSNSYLNGQSLSVV 185
Query: 611 VTASDRRTSTSWNAAPSTWQFGQTFEGKNF 700
VT + + S+N AP +W FGQT+ G+ F
Sbjct: 186 VTTGNGHSIVSFNVAPPSWSFGQTYTGRQF 275
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,757,859
Number of Sequences: 7669
Number of extensions: 81113
Number of successful extensions: 1219
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 279315504
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)