TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4005867.2.1
(1967 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U28148.1|MSU28148 Medicago sativa putative pectinesteras... 256 e-106
gb|CO514932.1|CO514932 s13dSG63B1100093_397369 Glandular tr... 203 8e-053
gb|CO514488.1|CO514488 s13dSG43C1100084_327636 Glandular tr... 110 8e-035
gb|AY587268.1| Medicago sativa pectin methylesterase (PEF) ... 134 6e-034
gb|CO517011.1|CO517011 s13dSG31A0200005_468568 Glandular tr... 139 8e-034
gb|AY587267.1| Medicago sativa pectin methylesterase (PER) ... 133 4e-033
>gb|U28148.1|MSU28148 Medicago sativa putative pectinesterase mRNA, complete cds
Length = 1573
Score = 256 bits (553), Expect(5) = e-106
Identities = 107/214 (50%), Positives = 142/214 (66%)
Frame = +1 / +1
Query: 730 LASGNQPKPNAIVAKDGSGQFKSIQQAVDAVPKGHQGRYVIYVKAGLYDEIVMVPKDKVN 909
L +G +PNA+VA+DGSGQFK++ A+ VP + +VI+VKAG+Y E V V K+
Sbjct: 442 LLAGGNVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKETVNVAKEMNY 621
Query: 910 IFMYGDGPKQSRVTGRKSFADGITTMKTATFSVEASGFICKNMGFHNTAGAERHQAVALR 1089
+ + GDGP +++ TG ++ADGI T TATF V + F+ K++GF NTAG +HQAVALR
Sbjct: 622 VTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGKHQAVALR 801
Query: 1090 VQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVVSGTIDFIFGNSAAVFQNCLIITRRPM 1269
V D A FYNC+ D FQDTLYV ++RQF+R+C +SGTIDF+FG VFQNC ++ R P
Sbjct: 802 VTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCKLVCRLPA 981
Query: 1270 DNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKL 1371
QQ VTA GR N S LV Q+ + L
Sbjct: 982 KGQQCLVTAGGREKQNSASALVFQSSHFTGEPAL 1083
Score = 130 bits (278), Expect(3) = 2e-048
Identities = 74/167 (44%), Positives = 86/167 (51%)
Frame = -1 / -3
Query: 1346 QFWMTSPDFMLGSVRPCAVTEFCWLSMGRRVMMRQFWNTAAELPKMKSMVPETTQLRKNC 1167
+ W T + RP AVT CW G QFW T PK KS+VPE Q R N
Sbjct: 1058 ELWKTRALALFCFSRPPAVTRHCWPFAGNLQTSLQFWKTPKRSPKTKSIVPEMLQSR*NW 879
Query: 1166 RRACTYSVSWNASNRQL*NAARSPCTRSATAWCRSAPAVLWNPMFLQMNPDASTEKVAVF 987
R T SVSW S QL*N A S TR ATA C PAV P+ M T VAV
Sbjct: 878 RWDWT*SVSWKPSI*QL*NIAWSAVTRRATA*CLPVPAVFSKPISFAMKFAPLTPNVAVL 699
Query: 986 MVVMPSAKLLRPVTRLCLGPSPYMKMLTLSLGTMTISS*RPAFT*MT 846
V++PSA L PV + +GPSP +SL T+T+S * PA T*+T
Sbjct: 698 *VLIPSA*LRLPVNLVLVGPSPMTVT*FISLATLTVSL*TPALT*IT 558
Score = 106 bits (226), Expect(5) = e-106
Identities = 42/82 (51%), Positives = 53/82 (64%)
Frame = +1 / +1
Query: 1396 SYLGRPWKEFSRLVIMESTIADFVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGTSKRVN 1575
SYLGRPWK++S++VIM+STI PEGYMPW G +T Y EYNN+GPGA T+ RV
Sbjct: 1105 SYLGRPWKQYSKVVIMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVK 1284
Query: 1576 WPGFHVIGRKEAEPFTAGPFID 1641
W G V+ A + G F +
Sbjct: 1285 WHGVKVLTSNVAAEYYPGKFFE 1350
Score = 62.5 bits (130), Expect(3) = 2e-048
Identities = 36/80 (45%), Positives = 42/80 (52%)
Frame = -1 / -3
Query: 1640 SMNGPAVNGSASFRPMTWKPGQLTLLLVPAPGPRLLYSA**SVLRAKSPFQGMYPSGLTK 1461
S N P +A+F T P LTL V APGP LLYS * V P GM PSG
Sbjct: 1349 SKNLPG*YSAATFEVKTLTPCHLTLKFVSAPGPLLLYS**VHVSLKALPIHGM*PSGTKI 1170
Query: 1460 SAMVLSMMTRRENSFQGRPR 1401
++MV S++T E F G PR
Sbjct: 1169 ASMVESIITTLEYCFHGLPR 1110
Score = 39.6 bits (80), Expect(5) = e-106
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = +1 / +1
Query: 427 DIKVLFSRSDDLETWLTGVMTFMDTCVDGFVDEKLKA 537
D L + DL+ WLTG ++ TC+DGF + KA
Sbjct: 154 DFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTTKA 264
Score = 34.1 bits (68), Expect(3) = 2e-048
Identities = 15/24 (62%), Positives = 16/24 (66%)
Frame = -1 / -3
Query: 824 GTASTACWMLLNCPLPSLATIAFG 753
GT A +LNCPLPS AT AFG
Sbjct: 536 GTVLRASVNVLNCPLPS*ATTAFG 465
Score = 31.8 bits (63), Expect(5) = e-106
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = +1 / +1
Query: 541 MHSVVRNATELSSNALAITNSLGGILKKMDLGMFSKDSR 657
M V++ + ELSSNA+ + +++ ILK D +S R
Sbjct: 274 MTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRR 390
Score = 25.8 bits (50), Expect(5) = e-106
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = +1 / +1
Query: 685 EKGWPVWMRSPERKLLASGN 744
+ G P W+ R+LLA GN
Sbjct: 400 DDGIPSWVNDGHRRLLAGGN 459
>gb|CO514932.1|CO514932 s13dSG63B1100093_397369 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 461
Score = 203 bits (437), Expect = 8e-053
Identities = 78/152 (51%), Positives = 101/152 (66%)
Frame = +1 / +2
Query: 1069 HQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVVSGTIDFIFGNSAAVFQNCL 1248
HQAVALRV D A Y C +QDT+Y H+ RQF+R C + GT+DFIFGN+A VFQNC
Sbjct: 5 HQAVALRVGSDHAVVYRCNVIGYQDTMYAHSNRQFYRECDIYGTVDFIFGNAAVVFQNCS 184
Query: 1249 IITRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLFPDRFKIPSYLGRPWKEFS 1428
+ R+PM Q+N++TA R DPN +G+ I NCR++ Q L + +YLGRPWK +S
Sbjct: 185 LYARKPMPYQKNTITAQNRKDPNQNTGISIHNCRILATQDLEASKGNFTTYLGRPWKLYS 364
Query: 1429 RLVIMESTIADFVKPEGYMPWNGDFALKTLYY 1524
R V M S + D + P G++ WN FAL TLYY
Sbjct: 365 RTVYMLSYMGDHIHPRGWLEWNTTFALDTLYY 460
>gb|CO514488.1|CO514488 s13dSG43C1100084_327636 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 592
Score = 110 bits (235), Expect(3) = 8e-035
Identities = 38/69 (55%), Positives = 53/69 (76%)
Frame = +1 / +3
Query: 1390 IPSYLGRPWKEFSRLVIMESTIADFVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGTSKR 1569
+ S+LGRPW+++SR V M++ I F+ P G++PW+G+FAL TLYYAEY N G G+ TS R
Sbjct: 93 VKSHLGRPWQKYSRTVFMKTVIDGFINPAGWLPWDGNFALDTLYYAEYANTGSGSSTSNR 272
Query: 1570 VNWPGFHVI 1596
V W G+HV+
Sbjct: 273 VTWKGYHVL 299
Score = 36.3 bits (73), Expect(3) = 8e-035
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +1 / +3
Query: 1294 AHGRTDPNMKSGLVIQNCRLVPDQKLFPDRFK 1389
A GRTDPN +G++I N R+ L P K
Sbjct: 3 AQGRTDPNQNTGIIIHNSRVSAQSDLNPSSVK 98
Score = 34.1 bits (68), Expect(3) = 8e-035
Identities = 13/27 (48%), Positives = 15/27 (55%)
Frame = +1 / +3
Query: 1606 EAEPFTAGPFIDGAMWLKYTGAPHILG 1686
+A PFT G FI G W+ TG P G
Sbjct: 312 QASPFTVGNFIAGNSWIGNTGVPFTSG 392
>gb|AY587268.1| Medicago sativa pectin methylesterase (PEF) mRNA, partial cds
Length = 351
Score = 134 bits (288), Expect(2) = 6e-034
Identities = 55/102 (53%), Positives = 69/102 (67%)
Frame = +1 / +1
Query: 1066 RHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVVSGTIDFIFGNSAAVFQNC 1245
+HQAVALRV D A FYNC+ D FQDTLYV ++RQF+R+C +SGTIDF+FG++ VFQNC
Sbjct: 1 KHQAVALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGDAFGVFQNC 180
Query: 1246 LIITRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKL 1371
++ R P QQ VTA GR N S LV Q+ + L
Sbjct: 181 KLVCRLPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPAL 306
Score = 25.4 bits (49), Expect(2) = 6e-034
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1 / +1
Query: 1396 SYLGRPWK 1419
SYLGRPWK
Sbjct: 328 SYLGRPWK 351
>gb|CO517011.1|CO517011 s13dSG31A0200005_468568 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 540
Score = 139 bits (299), Expect = 8e-034
Identities = 60/127 (47%), Positives = 87/127 (68%)
Frame = +1 / +2
Query: 721 RKLLASGNQPKPNAIVAKDGSGQFKSIQQAVDAVPKGHQGRYVIYVKAGLYDEIVMVPKD 900
R L N+ P+A+VAKDGSGQ+K+I +A+ V K R+V+YVK G+Y E + + K+
Sbjct: 155 RLLAVVVNETVPDAVVAKDGSGQYKTIGEALKLVKKKSLQRFVVYVKKGVYVENIDLDKN 334
Query: 901 KVNIFMYGDGPKQSRVTGRKSFADGITTMKTATFSVEASGFICKNMGFHNTAGAERHQAV 1080
N+ +YGDG ++ V+G ++F DG T +TATF+V+ GFI K++ F NTAGA +HQAV
Sbjct: 335 TWNVMIYGDGMTETIVSGGRNFMDGTPTFETATFAVKGKGFIAKDIQFLNTAGASKHQAV 514
Query: 1081 ALRVQGD 1101
A+R D
Sbjct: 515 AMRSGSD 535
>gb|AY587267.1| Medicago sativa pectin methylesterase (PER) mRNA, partial cds
Length = 351
Score = 133 bits (285), Expect(2) = 4e-033
Identities = 50/102 (49%), Positives = 72/102 (70%)
Frame = +1 / +1
Query: 1066 RHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVVSGTIDFIFGNSAAVFQNC 1245
+HQAVALRV D A FYNC+ D +Q TL+ ++RQF+R+C +SGTID ++G++ AVFQNC
Sbjct: 1 KHQAVALRVTADKAIFYNCQIDGYQATLFAESQRQFYRDCSISGTIDMVYGDAFAVFQNC 180
Query: 1246 LIITRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKL 1371
+I R+P++ QQ V A GRT + SG V Q+C + ++
Sbjct: 181 KLIVRKPLEEQQCFVAADGRTKSDSSSGFVFQSCHFTGEPEV 306
Score = 24.0 bits (46), Expect(2) = 4e-033
Identities = 7/8 (87%), Positives = 8/8 (100%)
Frame = +1 / +1
Query: 1396 SYLGRPWK 1419
+YLGRPWK
Sbjct: 328 AYLGRPWK 351
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,360,564
Number of Sequences: 7669
Number of extensions: 151700
Number of successful extensions: 979
Number of sequences better than 1.0e-020: 6
length of database: 1,248,568
effective HSP length: 45
effective length of database: 903,463
effective search space used: 551112430
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)