TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3977955.2.1
(867 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514932.1|CO514932 s13dSG63B1100093_397369 Glandular tr... 120 8e-046
gb|U28148.1|MSU28148 Medicago sativa putative pectinesteras... 95 1e-038
gb|CO514488.1|CO514488 s13dSG43C1100084_327636 Glandular tr... 123 9e-031
gb|AY587267.1| Medicago sativa pectin methylesterase (PER) ... 97 3e-021
gb|AY587268.1| Medicago sativa pectin methylesterase (PEF) ... 96 6e-021
>gb|CO514932.1|CO514932 s13dSG63B1100093_397369 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 461
Score = 120 bits (256), Expect(2) = 8e-046
Identities = 46/91 (50%), Positives = 58/91 (63%)
Frame = -2 / +2
Query: 866 YQCTFDGYQDTLYTHAQRQFFRDCRVTGTIDFIFGNSQVVLQNCLIQPRKPMANQANIIT 687
Y+C GYQDT+Y H+ RQF+R+C + GT+DFIFGN+ VV QNC + RKPM Q N IT
Sbjct: 50 YRCNVIGYQDTMYAHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSLYARKPMPYQKNTIT 229
Query: 686 AQGRRDKRSVGGTVLHNCTIEPHPDFKEEAG 594
AQ R+D G +HNC I D + G
Sbjct: 230 AQNRKDPNQNTGISIHNCRILATQDLEASKG 322
Score = 78.5 bits (165), Expect(2) = 8e-046
Identities = 28/51 (54%), Positives = 36/51 (70%)
Frame = -2 / +2
Query: 605 EEAGGKIRTYLARPWKEYSRTIYIQNDIGGFIDPKGWLEWNGDFGLETLFY 453
E + G TYL RPWK YSRT+Y+ + +G I P+GWLEWN F L+TL+Y
Sbjct: 308 EASKGNFTTYLGRPWKLYSRTVYMLSYMGDHIHPRGWLEWNTTFALDTLYY 460
>gb|U28148.1|MSU28148 Medicago sativa putative pectinesterase mRNA, complete cds
Length = 1573
Score = 94.5 bits (200), Expect(2) = 1e-038
Identities = 37/84 (44%), Positives = 48/84 (57%)
Frame = -2 / +1
Query: 866 YQCTFDGYQDTLYTHAQRQFFRDCRVTGTIDFIFGNSQVVLQNCLIQPRKPMANQANIIT 687
Y C DG+QDTLY +QRQF+RDC ++GTIDF+FG V QNC + R P Q ++T
Sbjct: 826 YNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCKLVCRLPAKGQQCLVT 1005
Query: 686 AQGRRDKRSVGGTVLHNCTIEPHP 615
A GR + S V + P
Sbjct: 1006 AGGREKQNSASALVFQSSHFTGEP 1077
Score = 79.9 bits (168), Expect(2) = 1e-038
Identities = 30/81 (37%), Positives = 47/81 (58%)
Frame = -2 / +1
Query: 581 TYLARPWKEYSRTIYIQNDIGGFIDPKGWLEWNGDFGLETLFYAEVDNRGDGADMSKRAK 402
+YL RPWK+YS+ + + + I P+G++ W G ET Y E +N+G GAD + R K
Sbjct: 1105 SYLGRPWKQYSKVVIMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVK 1284
Query: 401 WGGIKTVTYEEAQKEFTVETF 339
W G+K +T A + + + F
Sbjct: 1285 WHGVKVLTSNVAAEYYPGKFF 1347
>gb|CO514488.1|CO514488 s13dSG43C1100084_327636 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 592
Score = 123 bits (263), Expect(2) = 9e-031
Identities = 46/101 (45%), Positives = 64/101 (63%)
Frame = -2 / +3
Query: 587 IRTYLARPWKEYSRTIYIQNDIGGFIDPKGWLEWNGDFGLETLFYAEVDNRGDGADMSKR 408
++++L RPW++YSRT++++ I GFI+P GWL W+G+F L+TL+YAE N G G+ S R
Sbjct: 93 VKSHLGRPWQKYSRTVFMKTVIDGFINPAGWLPWDGNFALDTLYYAEYANTGSGSSTSNR 272
Query: 407 AKWGGIKTVTYEEAQKEFTVETFIQGQQFIPKFGVPFIPGL 285
W G +T FTV FI G +I GVPF GL
Sbjct: 273 VTWKGYHVLTSASQASPFTVGNFIAGNSWIGNTGVPFTSGL 395
Score = 24.9 bits (48), Expect(2) = 9e-031
Identities = 9/25 (36%), Positives = 12/25 (48%)
Frame = -2 / +3
Query: 686 AQGRRDKRSVGGTVLHNCTIEPHPD 612
AQGR D G ++HN + D
Sbjct: 3 AQGRTDPNQNTGIIIHNSRVSAQSD 77
>gb|AY587267.1| Medicago sativa pectin methylesterase (PER) mRNA, partial cds
Length = 351
Score = 96.8 bits (205), Expect = 3e-021
Identities = 36/85 (42%), Positives = 50/85 (58%)
Frame = -2 / +1
Query: 866 YQCTFDGYQDTLYTHAQRQFFRDCRVTGTIDFIFGNSQVVLQNCLIQPRKPMANQANIIT 687
Y C DGYQ TL+ +QRQF+RDC ++GTID ++G++ V QNC + RKP+ Q +
Sbjct: 49 YNCQIDGYQATLFAESQRQFYRDCSISGTIDMVYGDAFAVFQNCKLIVRKPLEEQQCFVA 228
Query: 686 AQGRRDKRSVGGTVLHNCTIEPHPD 612
A GR S G V +C P+
Sbjct: 229 ADGRTKSDSSSGFVFQSCHFTGEPE 303
>gb|AY587268.1| Medicago sativa pectin methylesterase (PEF) mRNA, partial cds
Length = 351
Score = 95.9 bits (203), Expect = 6e-021
Identities = 37/84 (44%), Positives = 50/84 (59%)
Frame = -2 / +1
Query: 866 YQCTFDGYQDTLYTHAQRQFFRDCRVTGTIDFIFGNSQVVLQNCLIQPRKPMANQANIIT 687
Y C DG+QDTLY +QRQF+RDC ++GTIDF+FG++ V QNC + R P Q ++T
Sbjct: 49 YNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGDAFGVFQNCKLVCRLPAKGQQCLVT 228
Query: 686 AQGRRDKRSVGGTVLHNCTIEPHP 615
A GR + S V + P
Sbjct: 229 AGGREKQNSASALVFQSSHFTGEP 300
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,524,325
Number of Sequences: 7669
Number of extensions: 77503
Number of successful extensions: 1883
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 227911620
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)