TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3848767.2.1
(1467 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO515300.1|CO515300 s13dSG48C1200098_417659 Glandular tr... 108 6e-049
gb|CO515609.1|CO515609 s13dSG47E0500039_418277 Glandular tr... 162 7e-045
gb|CO511782.1|CO511782 s13dSG02A0400033_103464 Glandular tr... 146 4e-040
gb|CO512362.1|CO512362 s13dSG80A0800053_114238 Glandular tr... 101 4e-034
emb|AJ245868.1|MSA245868 Medicago sativa mRNA for cysteine ... 64 4e-032
>gb|CO515300.1|CO515300 s13dSG48C1200098_417659 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 588
Score = 108 bits (230), Expect(5) = 6e-049
Identities = 38/57 (66%), Positives = 49/57 (85%)
Frame = -2 / +2
Query: 956 LPDAIDWRQLGAVTEVKDQQQCGGCWAFSAVAAIEGVNAIATGNLVSLSEQEIIDCD 786
LP+++DWR+ GAV VKDQ CG CWAFSA+AA+EG+N I TG+L+SLSEQE++DCD
Sbjct: 296 LPESVDWRKEGAVVGVKDQASCGSCWAFSAIAAVEGINKIVTGDLISLSEQELVDCD 466
Score = 77.1 bits (162), Expect(2) = 8e-022
Identities = 35/56 (62%), Positives = 37/56 (66%)
Frame = +1 / -1
Query: 787 SQSMISCSDSDTRLPVAIAFTPSMAATAENAQHPPHCCWSLTSVTAPSWRQSMASG 954
SQS SCSDS+ R PV I F PS AA AENAQ P WSLT TAPS+ QS SG
Sbjct: 465 SQSTSSCSDSEIRSPVTILFIPSTAAIAENAQQLPQLAWSLTPTTAPSFLQSTDSG 298
Score = 61.1 bits (127), Expect(5) = 6e-049
Identities = 21/37 (56%), Positives = 27/37 (72%)
Frame = -2 / +2
Query: 779 DSGCDGGQMENAFRFVIGNGGIDTEADYPFIGTDGTC 669
+ GC+GG M+ AF F+I NGGID+E DYP+ DG C
Sbjct: 476 NEGCNGGLMDYAFEFIISNGGIDSEEDYPYKAIDGRC 586
Score = 41.8 bits (85), Expect(5) = 6e-049
Identities = 17/23 (73%), Positives = 19/23 (82%)
Frame = -2 / +2
Query: 1091 TFRLGLTPFADLTLEEYRGRVLG 1023
T+RLGLT FADLT EEYR + LG
Sbjct: 146 TYRLGLTRFADLTNEEYRSKFLG 214
Score = 41.8 bits (85), Expect(2) = 8e-022
Identities = 19/36 (52%), Positives = 24/36 (66%)
Frame = +1 / -1
Query: 667 SQVPSVPMKG*SASVSIPPLPMTNRNAFSICPPSQP 774
S +PS+ + G*S+S S+PPL M N A SI PP P
Sbjct: 588 SHLPSIAL*G*SSSESMPPLLMMNSKA*SISPPLHP 481
Score = 28.1 bits (55), Expect(5) = 6e-049
Identities = 8/13 (61%), Positives = 12/13 (92%)
Frame = -2 / +2
Query: 1154 VFRDNLRYIDAHN 1116
+F+DNL++ID HN
Sbjct: 95 IFKDNLKFIDEHN 133
Score = 24.4 bits (47), Expect(5) = 6e-049
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = -2 / +2
Query: 1286 DEEVRRMYEAWKSKHGR 1236
+EEV M E W KHG+
Sbjct: 5 NEEVLTMCEEWLVKHGK 55
>gb|CO515609.1|CO515609 s13dSG47E0500039_418277 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 591
Score = 162 bits (349), Expect(2) = 7e-045
Identities = 62/98 (63%), Positives = 77/98 (78%)
Frame = -2 / +1
Query: 653 KNEKVATIDGLVEVASNNETALQEAVAIQPVSVAIDAGGRAFQHYSSGIFNGPCGTSLDH 474
KN KV TID +V + +E ALQ+AVA QP++VA++ GGR FQ Y G+F G CGT+LDH
Sbjct: 76 KNAKVVTIDDYEDVPAYDELALQKAVANQPIAVAVEGGGREFQLYEYGVFTGRCGTALDH 255
Query: 473 GVTAVGYGSESGKDYWIVKNSWSASWGEAGYIRMRRNV 360
GV AVGYG+E+GKDYWIV+NSW SWGE GYIR+ RN+
Sbjct: 256 GVAAVGYGTENGKDYWIVRNSWGGSWGEQGYIRLERNL 369
Score = 99.1 bits (210), Expect(2) = 6e-023
Identities = 48/97 (49%), Positives = 53/97 (54%)
Frame = +1 / -2
Query: 364 LRLMRM*PASPQLALHEFFTIQXXXXXXXXXXTAVTPWSRLVPHGPLKMPEL*CWNARPP 543
LR M*P SPQL HEF TIQ TA TPWS VPH P+ P WN+ PP
Sbjct: 365 LRSSLM*PCSPQLPPHEFLTIQ*SFPFSVPYPTAATPWSSAVPHLPVNTPYSYNWNSLPP 186
Query: 544 ASMATLTGWIATASCSAVSLLLATSTNPSMVATFSFF 654
S AT GW ATA C A S TS+ S+V T +FF
Sbjct: 185 PSTATAIGWFATAFCKANSS*AGTSS*SSIVTTLAFF 75
Score = 33.6 bits (67), Expect(2) = 7e-045
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = -2 / +1
Query: 347 GKCGIAMDASYPVKDTYHDP 288
GKCGIA++ SYP+K+ + P
Sbjct: 385 GKCGIAIEPSYPIKNGQNPP 444
Score = 23.5 bits (45), Expect(2) = 6e-023
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = +1 / -2
Query: 313 G*DASMAMPHLP 348
G*D S+A+PH P
Sbjct: 419 G*DGSIAIPHFP 384
>gb|CO511782.1|CO511782 s13dSG02A0400033_103464 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 536
Score = 146 bits (314), Expect(2) = 4e-040
Identities = 54/80 (67%), Positives = 66/80 (82%)
Frame = -2 / +2
Query: 599 ETALQEAVAIQPVSVAIDAGGRAFQHYSSGIFNGPCGTSLDHGVTAVGYGSESGKDYWIV 420
E ALQ+AVA QP++VA++ GGR FQ Y G+F G CGT+LDHGV AVGYG+E+GKDYWIV
Sbjct: 5 ELALQKAVANQPIAVAVEGGGREFQLYEYGVFTGRCGTALDHGVAAVGYGTENGKDYWIV 184
Query: 419 KNSWSASWGEAGYIRMRRNV 360
+NSW SWGE GYIR+ RN+
Sbjct: 185 RNSWGGSWGEQGYIRLERNL 244
Score = 33.6 bits (67), Expect(2) = 4e-040
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = -2 / +2
Query: 347 GKCGIAMDASYPVKDTYHDP 288
GKCGIA++ SYP+K+ + P
Sbjct: 260 GKCGIAIEPSYPIKNGQNPP 319
>gb|CO512362.1|CO512362 s13dSG80A0800053_114238 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 576
Score = 101 bits (215), Expect(3) = 4e-034
Identities = 37/58 (63%), Positives = 45/58 (77%)
Frame = -2 / +1
Query: 959 DLPDAIDWRQLGAVTEVKDQQQCGGCWAFSAVAAIEGVNAIATGNLVSLSEQEIIDCD 786
+LP+ DWR+ GAVT VKDQ CG CWAFS A+EG N +ATG LVSLSEQ+++DCD
Sbjct: 274 NLPEDFDWREKGAVTPVKDQGSCGSCWAFSTTGALEGANYLATGKLVSLSEQQLVDCD 447
Score = 49.2 bits (101), Expect(3) = 4e-034
Identities = 16/32 (50%), Positives = 24/32 (75%)
Frame = -2 / +1
Query: 779 DSGCDGGQMENAFRFVIGNGGIDTEADYPFIG 684
DSGC+GG M NAF +++ +GG+ +E DY + G
Sbjct: 481 DSGCNGGLMNNAFEYILQSGGVVSEKDYAYTG 576
Score = 27.6 bits (54), Expect(3) = 4e-034
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = -2 / +1
Query: 1079 GLTPFADLTLEEYRGRVLG 1023
G+T F+DLT E+R + LG
Sbjct: 157 GITKFSDLTASEFRRQFLG 213
>emb|AJ245868.1|MSA245868 Medicago sativa mRNA for cysteine protease, partial (cyp15a gene)
Length = 890
Score = 63.8 bits (133), Expect(4) = 4e-032
Identities = 23/36 (63%), Positives = 28/36 (77%)
Frame = -2 / +1
Query: 893 CGGCWAFSAVAAIEGVNAIATGNLVSLSEQEIIDCD 786
CG WAFS A+EG N +ATG LVSLSEQ+++DCD
Sbjct: 1 CGSGWAFSTTGALEGANYLATGKLVSLSEQQLVDCD 108
Score = 62.0 bits (129), Expect(4) = 4e-032
Identities = 21/43 (48%), Positives = 30/43 (69%)
Frame = -2 / +1
Query: 779 DSGCDGGQMENAFRFVIGNGGIDTEADYPFIGTDGTCDASKEK 651
DSGC+GG M NAF +++ +GG+ +E DY + G DG+C K K
Sbjct: 142 DSGCNGGLMNNAFEYILQSGGVVSEKDYAYTGRDGSCKFDKSK 270
Score = 45.5 bits (93), Expect(4) = 4e-032
Identities = 14/24 (58%), Positives = 18/24 (75%)
Frame = -2 / +1
Query: 437 KDYWIVKNSWSASWGEAGYIRMRR 366
K YWI+KNSW +WGE GY ++ R
Sbjct: 499 KPYWIIKNSWGQNWGEEGYYKICR 570
Score = 25.4 bits (49), Expect(4) = 4e-032
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -2 / +1
Query: 482 LDHGVTAVGYGS 447
LDHGV VG+GS
Sbjct: 433 LDHGVLLVGFGS 468
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,572,850
Number of Sequences: 7669
Number of extensions: 82297
Number of successful extensions: 877
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 404542608
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)