TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3829501.2.1
(1141 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA... 305 e-136
gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA... 305 e-136
gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, comple... 305 e-122
gb|CO514897.1|CO514897 s13dSG56F1200103_328454 Glandular tr... 85 3e-032
gb|CO516293.1|CO516293 s13dSG69F0900077_445914 Glandular tr... 65 2e-024
gb|CO515476.1|CO515476 s13dSG52A0400033_418011 Glandular tr... 65 9e-021
>gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA, complete cds
Length = 1267
Score = 305 bits (660), Expect(3) = e-136
Identities = 112/147 (76%), Positives = 130/147 (88%)
Frame = -2 / +2
Query: 597 YCEPSAQWPCAAGKKYYGRGPIQISYNYNYGPAGQAIGAGILANPDLVATDPTVSFETAV 418
YC+PS+++PCA+GK+YYGRGPIQIS+NYNYG G+AIG +L NPDLVATDP +SF+TA+
Sbjct: 527 YCQPSSEFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTAL 706
Query: 417 WFWMTPQSPKPSCHDVMTGQWTPSAADTAAGRLPGYGVVTNIINGGLECGHGADSRVADR 238
WFWMTPQSPKPSCHDV+TG+W+PS+AD AAGRL GYG VTNIINGGLECG G D RV DR
Sbjct: 707 WFWMTPQSPKPSCHDVITGRWSPSSADRAAGRLSGYGTVTNIINGGLECGRGQDGRVQDR 886
Query: 237 IGFYKRYCDLLGVSYGDNLDCANQTPF 157
IGFYKRYCD+LGV YG NLDC +Q PF
Sbjct: 887 IGFYKRYCDILGVGYGANLDCFSQRPF 967
Score = 149 bits (321), Expect(3) = e-136
Identities = 57/91 (62%), Positives = 64/91 (70%)
Frame = -2 / +2
Query: 891 GGVASIIPESLFNQMLLHRNDAACPANGFYTYAGFIAAANAFPGFGTTGAPDVQKRELAA 712
G + S+I FN ML HR+D+ C G YTY FI+AA AFP F G +KRE+AA
Sbjct: 242 GDLGSLISRDTFNNMLKHRDDSGCQGKGLYTYDAFISAAKAFPNFANNGDTATKKREIAA 421
Query: 711 FLAQTSHETTGGWATAPDGPYAWGYCFKEEQ 619
FL QTSHETTGGWATAPDGPYAWGYCF EQ
Sbjct: 422 FLGQTSHETTGGWATAPDGPYAWGYCFVREQ 514
Score = 85.8 bits (181), Expect(5) = 1e-049
Identities = 37/50 (74%), Positives = 39/50 (78%)
Frame = +2 / -2
Query: 320 SLPAAVSAAEGVHCPVMTSWHDGLGDCGVIQNQTAVSNDTVGSVATRSGL 469
SLPAA SA EG+H PV+TSW DGLGD GVIQN AV D GSVATRSGL
Sbjct: 804 SLPAARSALEGLHLPVITSWQDGLGDWGVIQNHRAVLKDITGSVATRSGL 655
Score = 67.0 bits (140), Expect(3) = e-136
Identities = 23/27 (85%), Positives = 24/27 (88%)
Frame = -1 / +2
Query: 1048 AEQCGSQAGGALCPNCLCCSQFGWCGS 968
AEQCG QAGGALCP LCCS+FGWCGS
Sbjct: 101 AEQCGKQAGGALCPGGLCCSKFGWCGS 181
Score = 65.2 bits (136), Expect(5) = 1e-049
Identities = 30/50 (60%), Positives = 35/50 (70%)
Frame = +2 / -2
Query: 620 CSSLKQ*PQA*GPSGAVAHPPVVSWDVCARNAASSRFCTSGAPVVPKPGN 769
CS KQ PQA GPSGAVAHPPVVS DV + AA S F + +P++ K GN
Sbjct: 513 CSLTKQYPQAYGPSGAVAHPPVVSCDV*PKKAAISLFLVAVSPLLAKLGN 364
Score = 52.4 bits (108), Expect(5) = 1e-049
Identities = 18/26 (69%), Positives = 20/26 (76%)
Frame = +3 / -2
Query: 975 HHPNWLQHRQLGHSAPPACDPHCSAR 1052
H PN LQH GH+APPAC PHCSA+
Sbjct: 174 HQPNLLQHNPPGHNAPPACLPHCSAK 97
Score = 42.3 bits (86), Expect(5) = 1e-049
Identities = 18/28 (64%), Positives = 20/28 (71%)
Frame = +2 / -2
Query: 512 L*L*DIWMGPRP*YFFPAAHGHWALGSQ 595
L* +IWMGP P*Y FP AHG+ LG Q
Sbjct: 612 L*FQEIWMGPLP*YCFPEAHGNSELGWQ 529
Score = 29.5 bits (58), Expect(5) = 1e-049
Identities = 13/20 (65%), Positives = 13/20 (65%)
Frame = +2 / -2
Query: 218 YRL*KPIRSATRLSAPWPHS 277
Y L* PIRS T S P PHS
Sbjct: 906 YLL*NPIRSCTLPSCPLPHS 847
>gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA, complete cds
Length = 1259
Score = 305 bits (660), Expect(3) = e-136
Identities = 112/147 (76%), Positives = 130/147 (88%)
Frame = -2 / +1
Query: 597 YCEPSAQWPCAAGKKYYGRGPIQISYNYNYGPAGQAIGAGILANPDLVATDPTVSFETAV 418
YC+PS+++PCA+GK+YYGRGPIQIS+NYNYG G+AIG +L NPDLVATDP +SF+TA+
Sbjct: 526 YCQPSSEFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTAL 705
Query: 417 WFWMTPQSPKPSCHDVMTGQWTPSAADTAAGRLPGYGVVTNIINGGLECGHGADSRVADR 238
WFWMTPQSPKPSCHDV+TG+W+PS+AD AAGRL GYG VTNIINGGLECG G D RV DR
Sbjct: 706 WFWMTPQSPKPSCHDVITGRWSPSSADRAAGRLSGYGTVTNIINGGLECGRGQDGRVQDR 885
Query: 237 IGFYKRYCDLLGVSYGDNLDCANQTPF 157
IGFYKRYCD+LGV YG NLDC +Q PF
Sbjct: 886 IGFYKRYCDILGVGYGANLDCFSQRPF 966
Score = 149 bits (321), Expect(3) = e-136
Identities = 57/91 (62%), Positives = 64/91 (70%)
Frame = -2 / +1
Query: 891 GGVASIIPESLFNQMLLHRNDAACPANGFYTYAGFIAAANAFPGFGTTGAPDVQKRELAA 712
G + S+I FN ML HR+D+ C G YTY FI+AA AFP F G +KRE+AA
Sbjct: 241 GDLGSLISRDTFNNMLKHRDDSGCQGKGLYTYDAFISAAKAFPNFANNGDTATKKREIAA 420
Query: 711 FLAQTSHETTGGWATAPDGPYAWGYCFKEEQ 619
FL QTSHETTGGWATAPDGPYAWGYCF EQ
Sbjct: 421 FLGQTSHETTGGWATAPDGPYAWGYCFVREQ 513
Score = 85.8 bits (181), Expect(5) = 1e-049
Identities = 37/50 (74%), Positives = 39/50 (78%)
Frame = +2 / -1
Query: 320 SLPAAVSAAEGVHCPVMTSWHDGLGDCGVIQNQTAVSNDTVGSVATRSGL 469
SLPAA SA EG+H PV+TSW DGLGD GVIQN AV D GSVATRSGL
Sbjct: 803 SLPAARSALEGLHLPVITSWQDGLGDWGVIQNHRAVLKDITGSVATRSGL 654
Score = 67.0 bits (140), Expect(3) = e-136
Identities = 23/27 (85%), Positives = 24/27 (88%)
Frame = -1 / +1
Query: 1048 AEQCGSQAGGALCPNCLCCSQFGWCGS 968
AEQCG QAGGALCP LCCS+FGWCGS
Sbjct: 100 AEQCGKQAGGALCPGGLCCSKFGWCGS 180
Score = 65.2 bits (136), Expect(5) = 1e-049
Identities = 30/50 (60%), Positives = 35/50 (70%)
Frame = +2 / -1
Query: 620 CSSLKQ*PQA*GPSGAVAHPPVVSWDVCARNAASSRFCTSGAPVVPKPGN 769
CS KQ PQA GPSGAVAHPPVVS DV + AA S F + +P++ K GN
Sbjct: 512 CSLTKQYPQAYGPSGAVAHPPVVSCDV*PKKAAISLFLVAVSPLLAKLGN 363
Score = 52.4 bits (108), Expect(5) = 1e-049
Identities = 18/26 (69%), Positives = 20/26 (76%)
Frame = +3 / -1
Query: 975 HHPNWLQHRQLGHSAPPACDPHCSAR 1052
H PN LQH GH+APPAC PHCSA+
Sbjct: 173 HQPNLLQHNPPGHNAPPACLPHCSAK 96
Score = 42.3 bits (86), Expect(5) = 1e-049
Identities = 18/28 (64%), Positives = 20/28 (71%)
Frame = +2 / -1
Query: 512 L*L*DIWMGPRP*YFFPAAHGHWALGSQ 595
L* +IWMGP P*Y FP AHG+ LG Q
Sbjct: 611 L*FQEIWMGPLP*YCFPEAHGNSELGWQ 528
Score = 29.5 bits (58), Expect(5) = 1e-049
Identities = 13/20 (65%), Positives = 13/20 (65%)
Frame = +2 / -1
Query: 218 YRL*KPIRSATRLSAPWPHS 277
Y L* PIRS T S P PHS
Sbjct: 905 YLL*NPIRSCTLPSCPLPHS 846
>gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, complete cds
Length = 987
Score = 305 bits (660), Expect(4) = e-122
Identities = 112/147 (76%), Positives = 130/147 (88%)
Frame = -2 / +1
Query: 597 YCEPSAQWPCAAGKKYYGRGPIQISYNYNYGPAGQAIGAGILANPDLVATDPTVSFETAV 418
YC+PS+++PCA+GK+YYGRGPIQIS+NYNYG G+AIG +L NPDLVATDP +SF+TA+
Sbjct: 496 YCQPSSEFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTAL 675
Query: 417 WFWMTPQSPKPSCHDVMTGQWTPSAADTAAGRLPGYGVVTNIINGGLECGHGADSRVADR 238
WFWMTPQSPKPSCHDV+TG+W+PS+AD AAGRL GYG VTNIINGGLECG G D RV DR
Sbjct: 676 WFWMTPQSPKPSCHDVITGRWSPSSADRAAGRLSGYGTVTNIINGGLECGRGQDGRVQDR 855
Query: 237 IGFYKRYCDLLGVSYGDNLDCANQTPF 157
IGFYKRYCD+LGV YG NLDC +Q PF
Sbjct: 856 IGFYKRYCDILGVGYGANLDCFSQRPF 936
Score = 85.8 bits (181), Expect(5) = 4e-041
Identities = 37/50 (74%), Positives = 39/50 (78%)
Frame = +2 / -2
Query: 320 SLPAAVSAAEGVHCPVMTSWHDGLGDCGVIQNQTAVSNDTVGSVATRSGL 469
SLPAA SA EG+H PV+TSW DGLGD GVIQN AV D GSVATRSGL
Sbjct: 773 SLPAARSALEGLHLPVITSWQDGLGDWGVIQNHRAVLKDITGSVATRSGL 624
Score = 71.6 bits (150), Expect(4) = e-122
Identities = 29/62 (46%), Positives = 35/62 (56%)
Frame = -2 / +1
Query: 891 GGVASIIPESLFNQMLLHRNDAACPANGFYTYAGFIAAANAFPGFGTTGAPDVQKRELAA 712
G + S+I FN ML HR+D+ C G YTY FI+AA AFP F G +KRE
Sbjct: 208 GDLGSLISRDTFNNMLKHRDDSGCQGKGLYTYDAFISAAKAFPNFANNGDTATKKREDCC 387
Query: 711 FL 706
FL
Sbjct: 388 FL 393
Score = 67.0 bits (140), Expect(4) = e-122
Identities = 23/27 (85%), Positives = 24/27 (88%)
Frame = -1 / +1
Query: 1048 AEQCGSQAGGALCPNCLCCSQFGWCGS 968
AEQCG QAGGALCP LCCS+FGWCGS
Sbjct: 67 AEQCGKQAGGALCPGGLCCSKFGWCGS 147
Score = 52.4 bits (108), Expect(5) = 4e-041
Identities = 18/26 (69%), Positives = 20/26 (76%)
Frame = +3 / -2
Query: 975 HHPNWLQHRQLGHSAPPACDPHCSAR 1052
H PN LQH GH+APPAC PHCSA+
Sbjct: 140 HQPNLLQHNPPGHNAPPACLPHCSAK 63
Score = 49.2 bits (101), Expect(4) = e-122
Identities = 17/20 (85%), Positives = 17/20 (85%)
Frame = -2 / +1
Query: 678 GWATAPDGPYAWGYCFKEEQ 619
G ATAPDGPYAWGYCF EQ
Sbjct: 424 GGATAPDGPYAWGYCFVREQ 483
Score = 42.3 bits (86), Expect(5) = 4e-041
Identities = 18/28 (64%), Positives = 20/28 (71%)
Frame = +2 / -2
Query: 512 L*L*DIWMGPRP*YFFPAAHGHWALGSQ 595
L* +IWMGP P*Y FP AHG+ LG Q
Sbjct: 581 L*FQEIWMGPLP*YCFPEAHGNSELGWQ 498
Score = 34.1 bits (68), Expect(5) = 4e-041
Identities = 15/20 (75%), Positives = 15/20 (75%)
Frame = +2 / -2
Query: 620 CSSLKQ*PQA*GPSGAVAHP 679
CS KQ PQA GPSGAVA P
Sbjct: 482 CSLTKQYPQAYGPSGAVAPP 423
Score = 29.5 bits (58), Expect(5) = 4e-041
Identities = 13/20 (65%), Positives = 13/20 (65%)
Frame = +2 / -2
Query: 218 YRL*KPIRSATRLSAPWPHS 277
Y L* PIRS T S P PHS
Sbjct: 875 YLL*NPIRSCTLPSCPLPHS 816
>gb|CO514897.1|CO514897 s13dSG56F1200103_328454 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 510
Score = 84.9 bits (179), Expect(3) = 3e-032
Identities = 28/59 (47%), Positives = 42/59 (71%)
Frame = -2 / +1
Query: 576 WPCAAGKKYYGRGPIQISYNYNYGPAGQAIGAGILANPDLVATDPTVSFETAVWFWMTP 400
+PC G +YYGRG I I +NYNYG AG+A+ +L +P+ + + T++F+ A+W WMTP
Sbjct: 46 YPCTPGAEYYGRGAIPIYWNYNYGAAGEALKVNLLDHPEYIEQNATLAFQAAIWKWMTP 222
Score = 64.3 bits (134), Expect(3) = 3e-032
Identities = 26/64 (40%), Positives = 33/64 (51%)
Frame = -2 / +1
Query: 390 KPSCHDVMTGQWTPSAADTAAGRLPGYGVVTNIINGGLECGHGADSRVADRIGFYKRYCD 211
+PS HD G W P+ DT R+PG+G NI+ G CG G + + Y Y D
Sbjct: 235 QPSAHDAFVGNWKPTKNDTMEKRVPGFGATMNILYGEGVCGQGDVDSMNTIVSHYLYYLD 414
Query: 210 LLGV 199
LLGV
Sbjct: 415 LLGV 426
Score = 22.1 bits (42), Expect(3) = 3e-032
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = -2 / +1
Query: 189 DNLDCANQTPFN 154
D L CA Q PFN
Sbjct: 451 DVLTCAEQRPFN 486
>gb|CO516293.1|CO516293 s13dSG69F0900077_445914 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 520
Score = 65.2 bits (136), Expect(3) = 2e-024
Identities = 26/64 (40%), Positives = 34/64 (53%)
Frame = -2 / +3
Query: 390 KPSCHDVMTGQWTPSAADTAAGRLPGYGVVTNIINGGLECGHGADSRVADRIGFYKRYCD 211
+PS HD G W P+ DT R+PG+G NI+ G CG G + + + Y Y D
Sbjct: 138 QPSAHDAFVGNWKPTKNDTMEKRVPGFGATMNILYGEGVCGQGDVDSMNNIVSHYLYYLD 317
Query: 210 LLGV 199
LLGV
Sbjct: 318 LLGV 329
Score = 57.4 bits (119), Expect(3) = 2e-024
Identities = 18/40 (45%), Positives = 29/40 (72%)
Frame = -2 / +3
Query: 519 NYNYGPAGQAIGAGILANPDLVATDPTVSFETAVWFWMTP 400
NYNYG AG+A+ +L +P+ + + T++F+ A+W WMTP
Sbjct: 6 NYNYGAAGEALKVNLLDHPEYIEQNATLAFQAAIWKWMTP 125
Score = 22.1 bits (42), Expect(3) = 2e-024
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = -2 / +3
Query: 189 DNLDCANQTPFN 154
D L CA Q PFN
Sbjct: 354 DVLTCAEQRPFN 389
>gb|CO515476.1|CO515476 s13dSG52A0400033_418011 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 494
Score = 64.8 bits (135), Expect(3) = 9e-021
Identities = 26/64 (40%), Positives = 33/64 (51%)
Frame = -2 / +3
Query: 390 KPSCHDVMTGQWTPSAADTAAGRLPGYGVVTNIINGGLECGHGADSRVADRIGFYKRYCD 211
+PS HD G W P+ DT R+PG+G NI+ G CG G + + Y Y D
Sbjct: 126 QPSAHDAFVGNWKPTKNDTMEKRVPGFGATMNILYGEGVCGQGDVDSMNSIVSHYLYYLD 305
Query: 210 LLGV 199
LLGV
Sbjct: 306 LLGV 317
Score = 45.5 bits (93), Expect(3) = 9e-021
Identities = 14/36 (38%), Positives = 25/36 (69%)
Frame = -2 / +3
Query: 507 GPAGQAIGAGILANPDLVATDPTVSFETAVWFWMTP 400
G AG+A+ +L +P+ + + T++F+ A+W WMTP
Sbjct: 6 GAAGEALKVNLLDHPEYIEQNATLAFQAAIWKWMTP 113
Score = 22.1 bits (42), Expect(3) = 9e-021
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = -2 / +3
Query: 189 DNLDCANQTPFN 154
D L CA Q PFN
Sbjct: 342 DVLTCAEQRPFN 377
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,826,663
Number of Sequences: 7669
Number of extensions: 62441
Number of successful extensions: 503
Number of sequences better than 1.0e-020: 7
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 308717136
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)