TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3802544.2.1
(814 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO517063.1|CO517063 s13dSG31H0600058_468672 Glandular tr... 198 1e-077
gb|CO516412.1|CO516412 s13dSG27D0900076_446152 Glandular tr... 195 2e-058
gb|AF201458.1|AF201458 Medicago sativa isoflavone reductase... 112 4e-048
emb|X58078.1|MSISREDMR M.sativa mRNA for isoflavone reductase 150 2e-047
gb|U17436.1|MSU17436 Medicago sativa isoflavone reductase (... 82 1e-037
gb|CO512559.1|CO512559 s13dSG08E0900067_121044 Glandular tr... 139 3e-034
gb|CO515541.1|CO515541 s13dSG53C0200018_418141 Glandular tr... 123 3e-029
>gb|CO517063.1|CO517063 s13dSG31H0600058_468672 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 198 bits (426), Expect(2) = 1e-077
Identities = 76/113 (67%), Positives = 92/113 (81%)
Frame = -1 / +1
Query: 538 LPPKEKVHVYGDGNVKAIFCDEDDVATYTIKSVDDPRALNKTLYIRPPENILTQNDVISK 359
L +KV +YGDGNVK ++ DEDDVATYTIK++DDPR LNKT+YIRPPENILTQ +++ K
Sbjct: 154 LSSTDKVVLYGDGNVKVVYMDEDDVATYTIKTIDDPRTLNKTIYIRPPENILTQRELVEK 333
Query: 358 WEKLSGNVLEKIHIPADEFLASMKGTDLANQAGVGHYYHIFYEGCLTNFEIGD 200
WEKL+G LEK I +FL+SMKG DLA Q VGH+YHIF+EGCLTNFE+ D
Sbjct: 334 WEKLTGKQLEKSTISEQDFLSSMKGLDLAGQVAVGHFYHIFFEGCLTNFELKD 492
Score = 128 bits (275), Expect(2) = 2e-047
Identities = 65/107 (60%), Positives = 74/107 (69%)
Frame = +3 / -2
Query: 210 SKFVKQPS*KMW**CPTPA*LAKSVPFIEAKNSSAGMWIFSRTFPESFSHFEITSF*VKM 389
SKFVK PS K+ * CPT A+S PF+E + S + M FS FP SFSHF +S *V+M
Sbjct: 482 SKFVKHPSKKI**KCPTATWPARSKPFMEERKSCSDMVDFSSCFPVSFSHFSTSSL*VRM 303
Query: 390 FSGGLMYSVLFNARGSSTDLIVYVATSSSSQNIALTLPSPYT*TFSL 530
FSGGL+Y VLFN RGSS LIVYVATSSSS LTLPSP T S+
Sbjct: 302 FSGGLIYIVLFNVRGSSIVLIVYVATSSSSI*TTLTLPSP*RTTLSV 162
Score = 106 bits (227), Expect(2) = 1e-077
Identities = 41/57 (71%), Positives = 48/57 (84%)
Frame = -1 / +2
Query: 688 GHALEPGRVTFDEKMDLRRAIEDANIPHTYVSANCFAAYFCPNLCQMRTLLPPKEKV 518
GHALEPGRVTFD+KM +R+AIEDANIP TY+SANCFA YF NL QM TL PP+ ++
Sbjct: 5 GHALEPGRVTFDDKMAIRKAIEDANIPFTYISANCFAGYFAGNLSQMGTLFPPRTRL 175
Score = 97.7 bits (207), Expect(2) = 2e-026
Identities = 49/101 (48%), Positives = 65/101 (64%)
Frame = -3 / +3
Query: 512 IWRRQCQSYIL**RRCCNVYNQVC**SSRIEQDTIHKTTRKHLDSKRCYLEVGKAFRKRS 333
+WRR CQS + *R CCN++N **SS +EQD I+K TRKH SKR E+G+ +RK +
Sbjct: 180 LWRR*CQSCLYG*R*CCNIHN*DN**SSNVEQDNIYKATRKHPHSKRAS*EMGETYRKAT 359
Query: 332 GENPHSCR*ILGFNERY*LGQSSRSGTLLPHFL*RLLDKF* 210
E H + F+ER+ + S SG L +FL*R+ DKF*
Sbjct: 360 REVYHIRTRLSFFHERFGPCRPSSSGAFLSYFL*RVFDKF* 482
Score = 82.2 bits (173), Expect(2) = 1e-021
Identities = 47/107 (43%), Positives = 58/107 (54%)
Frame = -2 / +2
Query: 531 PKKKFMYMETAMSKLYSVMKTMLQRIQSSLLMILAH*TRHYT*DHQKTS*LKTMLSRSGK 352
P+ + M T MSKL+ MK MLQ Q LMIL *TR Y * HQKTS LK R+G+
Sbjct: 161 PRTRLFSMATVMSKLFIWMKMMLQHTQLRQLMILER*TRQYI*GHQKTSSLKES*LRNGR 340
Query: 351 SFPETFWRKSTFLQMNSWLQ*KVLTWPIKPEWDTITTFSMKVA*QIL 211
+ E+ R + +L *KV T K +W FS+K *QIL
Sbjct: 341 NLQESN*RSLPYPNKTFFLP*KVWTLQAK*QWGISIIFSLKGV*QIL 481
Score = 79.4 bits (167), Expect(3) = 2e-032
Identities = 33/65 (50%), Positives = 44/65 (67%)
Frame = +2 / -1
Query: 338 VSGKLFPLRDNIVLSQDVFWWSYV*CLVQCARIINRLDCIRCNIVFITEYSFDIAVSIYM 517
+S K P + + LS+DVFWW Y+ CLVQ +RIIN L+C+ CNI+FI +FDI V+I
Sbjct: 354 LSCKFLPFLN*LSLSEDVFWWPYIYCLVQRSRIINCLNCVCCNIIFIHINNFDITVAIEN 175
Query: 518 NFFFG 532
N G
Sbjct: 174 NLVRG 160
Score = 74.8 bits (157), Expect(2) = 2e-047
Identities = 39/53 (73%), Positives = 40/53 (75%)
Frame = +3 / -1
Query: 531 GGKRVRI*HRFGQK*AAKQLAET*V*GMLASSIARLRSIFSSNVTLPGSRACP 689
GGK V I* R K* AKQLAE *V GMLASSIA L +I SSNVTLPGS ACP
Sbjct: 162 GGKSVPI*DRLPAK*PAKQLAEM*VKGMLASSIAFLIAILSSNVTLPGSSACP 4
Score = 55.6 bits (115), Expect(3) = 2e-032
Identities = 22/51 (43%), Positives = 32/51 (62%)
Frame = +2 / -3
Query: 533 RQKGAHLT*VWAKVSSKAIGRNIGVRNVGIFYCTP*VHLFIKCNPSWFKSM 685
R++ +HL V KV+ + +G ++ R+VGI C P H IKC+ SWFK M
Sbjct: 160 RKECSHLRQVTGKVARETVGGDVSERDVGILNCFPYSHFIIKCDSSWFKCM 8
Score = 36.3 bits (73), Expect(3) = 2e-032
Identities = 14/31 (45%), Positives = 19/31 (61%)
Frame = +2 / -1
Query: 200 VADFKICQATFIENVVIVSHSGLIGQVSTFH 292
+ FKICQ F EN++ + H L +V TFH
Sbjct: 492 ILQFKICQTPFKENMIEMPHCYLACKVQTFH 400
Score = 35.9 bits (72), Expect(2) = 2e-026
Identities = 16/27 (59%), Positives = 19/27 (70%)
Frame = -2 / +3
Query: 684 MLLNQEGLHLMKRWT*GVQ*KMPTFLT 604
M LNQE HLM +W *G Q +MPT L+
Sbjct: 9 MHLNQEESHLMIKWL*GKQLRMPTSLS 89
Score = 35.0 bits (70), Expect(2) = 1e-021
Identities = 16/29 (55%), Positives = 19/29 (65%)
Frame = -3 / +1
Query: 689 GACS*TRKGYI**KDGPKACNRRCQHSSH 603
G C+*TRK +I** +G K N CQH H
Sbjct: 4 GTCT*TRKSHI***NGYKESN*GCQHPFH 90
>gb|CO516412.1|CO516412 s13dSG27D0900076_446152 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 456
Score = 195 bits (421), Expect(2) = 2e-058
Identities = 75/103 (72%), Positives = 87/103 (84%)
Frame = -1 / +3
Query: 508 GDGNVKAIFCDEDDVATYTIKSVDDPRALNKTLYIRPPENILTQNDVISKWEKLSGNVLE 329
GDGNVK +F DEDDVATYTIK++DDPR LNKT+Y+RPPENILTQ ++I +WEKL G LE
Sbjct: 6 GDGNVKVVFMDEDDVATYTIKTIDDPRTLNKTIYLRPPENILTQRELIERWEKLIGKQLE 185
Query: 328 KIHIPADEFLASMKGTDLANQAGVGHYYHIFYEGCLTNFEIGD 200
K I +FL+SMKG DLA+Q GVGH+YHIFYEGCL NFEIGD
Sbjct: 186 KSTISEQDFLSSMKGLDLASQVGVGHFYHIFYEGCLANFEIGD 314
Score = 142 bits (305), Expect(2) = 1e-038
Identities = 68/103 (66%), Positives = 75/103 (72%)
Frame = +3 / -3
Query: 201 SPISKFVKQPS*KMW**CPTPA*LAKSVPFIEAKNSSAGMWIFSRTFPESFSHFEITSF* 380
SPISKF K PS*K+ * CPTP LA+S PF+E + S + M FS FP SFSH I+S *
Sbjct: 313 SPISKFAKHPS*KI**KCPTPTWLARSKPFMEERKSCSDMVDFSSCFPISFSHLSISSL* 134
Query: 381 VKMFSGGLMYSVLFNARGSSTDLIVYVATSSSSQNIALTLPSP 509
V+MFSGGL Y VLFN RGSS LIVYVATSSSS LTLPSP
Sbjct: 133 VRMFSGGLRYIVLFNVRGSSIVLIVYVATSSSSIKTTLTLPSP 5
Score = 115 bits (245), Expect = 9e-027
Identities = 49/101 (48%), Positives = 69/101 (68%)
Frame = +2 / -2
Query: 209 FKICQATFIENVVIVSHSGLIGQVSTFH*SQEFICRNVDFLQNVSGKLFPLRDNIVLSQD 388
FKICQ FIEN++ + HS L +V TFH ++ + FL +S K P + + LS+D
Sbjct: 305 FKICQTPFIENMIEMPHSYLACKVQTFHGRKKVLFGYGRFL*LLSNKFLPSLN*LSLSED 126
Query: 389 VFWWSYV*CLVQCARIINRLDCIRCNIVFITEYSFDIAVSI 511
VFWW + CLVQC+R+IN L+C+ CNI+FI + +FDIAV+I
Sbjct: 125 VFWWP*IYCLVQCSRVINCLNCVCCNIIFIHKNNFDIAVAI 3
Score = 97.7 bits (207), Expect(2) = 1e-024
Identities = 51/102 (50%), Positives = 63/102 (61%)
Frame = -3 / +2
Query: 509 WRRQCQSYIL**RRCCNVYNQVC**SSRIEQDTIHKTTRKHLDSKRCYLEVGKAFRKRSG 330
WRRQCQS *R CCN++N ** S IEQD I K TRKH SKR E+G+ + K +
Sbjct: 5 WRRQCQSCFYG*R*CCNIHN*DN**PSNIEQDNISKATRKHPHSKRAN*EMGETYWKATR 184
Query: 329 ENPHSCR*ILGFNERY*LGQSSRSGTLLPHFL*RLLDKF*NR 204
E H + F+ER+ + SRSG L +FL*R+ KF*NR
Sbjct: 185 EIYHIRTRLSFFHERFGPCKPSRSGAFLSYFL*RVFGKF*NR 310
Score = 45.1 bits (92), Expect(2) = 2e-058
Identities = 18/26 (69%), Positives = 21/26 (80%)
Frame = -1 / +3
Query: 199 DGADATLLYPEVQYTRMDEYMKRYV* 122
DG +A+ LYPEVQ TRMDEY+K Y *
Sbjct: 312 DGEEASKLYPEVQCTRMDEYLKLYA* 389
Score = 32.2 bits (64), Expect(2) = 1e-038
Identities = 15/26 (57%), Positives = 19/26 (73%)
Frame = +3 / -3
Query: 123 YTYLFMYSSMRVY*TSGYNRVASAPS 200
+ Y F YSSMRV+ TSG + AS+PS
Sbjct: 388 HAYNFRYSSMRVHCTSG*SFDASSPS 311
Score = 29.5 bits (58), Expect(2) = 1e-024
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -3 / +2
Query: 203 RRWSRRYSVVPRGSVYPHG*VHEEVCV 123
R W R + RG+++ HG*+ + +CV
Sbjct: 308 R*WRRGIKALSRGAMHTHG*ISKIICV 388
>gb|AF201458.1|AF201458 Medicago sativa isoflavone reductase-like NAD(P)H-dependent
oxidoreductase (IRL1) mRNA, complete cds
Length = 1220
Score = 112 bits (239), Expect(4) = 4e-048
Identities = 45/110 (40%), Positives = 69/110 (62%)
Frame = -1 / +2
Query: 535 PPKEKVHVYGDGNVKAIFCDEDDVATYTIKSVDDPRALNKTLYIRPPENILTQNDVISKW 356
PPK+KV +YGDGN KA+F EDD+ T+TI++VD P NK LYI+PP+ + N++++ W
Sbjct: 539 PPKDKVVIYGDGNPKAVFNKEDDIGTFTIRAVDAPGPWNKILYIKPPKESYSSNELVALW 718
Query: 355 EKLSGNVLEKIHIPADEFLASMKGTDLANQAGVGHYYHIFYEGCLTNFEI 206
EK SG +K +P D L ++ + + ++ +F +G TNF I
Sbjct: 719 EKKSGKPAQKPSLPEDNLLKDIQEAPIPITVVLPIHHGVFVKGDHTNFVI 868
Score = 64.8 bits (135), Expect(4) = 4e-048
Identities = 27/55 (49%), Positives = 33/55 (60%)
Frame = -1 / +2
Query: 685 HALEPGRVTFDEKMDLRRAIEDANIPHTYVSANCFAAYFCPNLCQMRTLLPPKEK 521
HA++P + F K +RRAIE IP+TYVS+N FA YF P L Q PP K
Sbjct: 383 HAVDPAKSAFYGKAKIRRAIEAEGIPYTYVSSNYFAGYFLPTLAQPGQFAPPPPK 547
Score = 33.6 bits (67), Expect(4) = 4e-048
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -1 / +2
Query: 784 LSXQHXXVEAIKEAGNXXXFIPSEFGMDPSKM 689
+ Q + AIKEAGN F PS FG D ++
Sbjct: 287 IEDQVKIIAAIKEAGNVKRFFPSAFGNDVDRV 382
Score = 31.8 bits (63), Expect(4) = 4e-048
Identities = 12/25 (48%), Positives = 19/25 (76%)
Frame = -1 / +2
Query: 196 GADATLLYPEVQYTRMDEYMKRYV* 122
G +A LYP+V+YT ++EY+ + V*
Sbjct: 881 GVEAYELYPDVKYTTVEEYLDQSV* 955
>emb|X58078.1|MSISREDMR M.sativa mRNA for isoflavone reductase
Length = 1181
Score = 150 bits (322), Expect(3) = 2e-047
Identities = 59/132 (44%), Positives = 86/132 (65%)
Frame = -1 / +1
Query: 682 ALEPGRVTFDEKMDLRRAIEDANIPHTYVSANCFAAYFCPNLCQMRTLLPPKEKVHVYGD 503
A+EP R F+EK +RR IE +P+TY+ + F YF NL Q+ PP++KV + GD
Sbjct: 448 AVEPVRQVFEEKASIRRVIEAEGVPYTYLCCHAFTGYFLRNLAQLDATDPPRDKVVILGD 627
Query: 502 GNVKAIFCDEDDVATYTIKSVDDPRALNKTLYIRPPENILTQNDVISKWEKLSGNVLEKI 323
GNVK + E DV T+TI++ +DP LNK ++IR P+N LTQN+VI+ WEK G LEK
Sbjct: 628 GNVKGAYVTEADVGTFTIRAANDPNTLNKAVHIRLPKNYLTQNEVIALWEKKIGKTLEKT 807
Query: 322 HIPADEFLASMK 287
++ ++ L ++
Sbjct: 808 YVSEEQVLKDIQ 843
Score = 84.0 bits (177), Expect(2) = 9e-021
Identities = 53/131 (40%), Positives = 67/131 (51%)
Frame = +3 / -1
Query: 291 IEAKNSSAGMWIFSRTFPESFSHFEITSF*VKMFSGGLMYSVLFNARGSSTDLIVYVATS 470
I + S+ +FSR FP FS +TSF VK F G L+++ LFN GS L+V V TS
Sbjct: 839 ISLRTCSSET*VFSRVFPIFFSQRAMTSFWVK*FLGSLIWTALFNVLGSFAALMVKVPTS 660
Query: 471 SSSQNIALTLPSPYT*TFSLGGKRVRI*HRFGQK*AAKQLAET*V*GMLASSIARLRSIF 650
+S T PSP T S GG * + +K* K + *V*G ++SI F
Sbjct: 659 ASVT*APFTFPSPRMTTLSRGGSVASS*AKLRKK*PVKAWQQR*V*GTPSASITLRILAF 480
Query: 651 SSNVTLPGSRA 683
SS L GS A
Sbjct: 479 SSKTCLTGSTA 447
Score = 38.6 bits (78), Expect(3) = 2e-047
Identities = 15/28 (53%), Positives = 19/28 (67%)
Frame = -1 / +1
Query: 784 LSXQHXXVEAIKEAGNXXXFIPSEFGMD 701
+ Q ++AIKEAGN F PSEFG+D
Sbjct: 349 IEDQVKIIKAIKEAGNVKKFFPSEFGLD 432
Score = 33.1 bits (66), Expect(3) = 2e-047
Identities = 12/24 (50%), Positives = 18/24 (75%)
Frame = -1 / +1
Query: 190 DATLLYPEVQYTRMDEYMKRYV*R 119
+A+ YP+V YT DEY+ ++V*R
Sbjct: 940 EASEAYPDVTYTTADEYLNQFV*R 1011
Score = 30.4 bits (60), Expect(2) = 9e-021
Identities = 13/19 (68%), Positives = 13/19 (68%)
Frame = +3 / -1
Query: 702 SMPNSDGIKXXXFPASLIA 758
S PNSDG FPASLIA
Sbjct: 431 SSPNSDGKNFLTFPASLIA 375
>gb|U17436.1|MSU17436 Medicago sativa isoflavone reductase (IFR) gene, complete cds
Length = 2730
Score = 82.2 bits (173), Expect(4) = 1e-037
Identities = 31/71 (43%), Positives = 47/71 (66%)
Frame = -1 / +2
Query: 496 VKAIFCDEDDVATYTIKSVDDPRALNKTLYIRPPENILTQNDVISKWEKLSGNVLEKIHI 317
+ + E DV T+TI++ +DP LNK ++IR PEN LTQN+VI+ WEK G LEK ++
Sbjct: 1757 ISGAYVTEADVGTFTIRAANDPNTLNKAVHIRLPENYLTQNEVIALWEKKIGKTLEKTYV 1936
Query: 316 PADEFLASMKG 284
++ L ++G
Sbjct: 1937 SEEQVLKDIQG 1969
Score = 79.4 bits (167), Expect(4) = 1e-037
Identities = 31/64 (48%), Positives = 42/64 (65%)
Frame = -1 / +3
Query: 682 ALEPGRVTFDEKMDLRRAIEDANIPHTYVSANCFAAYFCPNLCQMRTLLPPKEKVHVYGD 503
A+EP R F+EK +RR IE +P+TY+ + F YF NL Q+ T PP++KV + GD
Sbjct: 1476 AVEPVRQVFEEKASIRRVIEAEGVPYTYLCCHAFTGYFLRNLAQLDTTDPPRDKVVILGD 1655
Query: 502 GNVK 491
GNVK
Sbjct: 1656 GNVK 1667
Score = 33.1 bits (66), Expect(4) = 1e-037
Identities = 12/24 (50%), Positives = 18/24 (75%)
Frame = -1 / +3
Query: 190 DATLLYPEVQYTRMDEYMKRYV*R 119
+A+ YP+V YT DEY+ ++V*R
Sbjct: 2295 EASEAYPDVTYTTADEYLNQFV*R 2366
Score = 22.1 bits (42), Expect(4) = 1e-037
Identities = 7/9 (77%), Positives = 8/9 (88%)
Frame = -1 / +3
Query: 727 FIPSEFGMD 701
F PSEFG+D
Sbjct: 1434 FFPSEFGLD 1460
>gb|CO512559.1|CO512559 s13dSG08E0900067_121044 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 573
Score = 139 bits (299), Expect = 3e-034
Identities = 59/92 (64%), Positives = 67/92 (72%)
Frame = -1 / +1
Query: 814 MSXXXXXXXXLSXQHXXVEAIKEAGNXXXFIPSEFGMDPSKMGHALEPGRVTFDEKMDLR 635
MS L Q +EAIK+A N F+PSEFGMDP+ MGHALEPGRVTFD+KM +R
Sbjct: 298 MSGVHFRSHNLMLQLKLIEAIKDAENVKRFLPSEFGMDPALMGHALEPGRVTFDDKMAIR 477
Query: 634 RAIEDANIPHTYVSANCFAAYFCPNLCQMRTL 539
+AIEDANIP TY+SANCFA YF NL QM TL
Sbjct: 478 KAIEDANIPFTYISANCFAGYFAGNLSQMGTL 573
Score = 99.1 bits (210), Expect = 6e-022
Identities = 51/78 (65%), Positives = 55/78 (70%)
Frame = +3 / -2
Query: 543 VRI*HRFGQK*AAKQLAET*V*GMLASSIARLRSIFSSNVTLPGSRACPILDGSMPNSDG 722
V I* R K* AKQLAE *V GMLASSIA L +I SSNVTLPGS ACPI GS+PNS+G
Sbjct: 569 VPI*ERLPAK*PAKQLAEM*VKGMLASSIAFLIAILSSNVTLPGSSACPIRAGSIPNSEG 390
Query: 723 IKXXXFPASLIASTXXCC 776
K F ASL+AS C
Sbjct: 389 RKRLTFSASLMASISLSC 336
>gb|CO515541.1|CO515541 s13dSG53C0200018_418141 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 539
Score = 123 bits (263), Expect = 3e-029
Identities = 52/79 (65%), Positives = 58/79 (73%)
Frame = -1 / +2
Query: 814 MSXXXXXXXXLSXQHXXVEAIKEAGNXXXFIPSEFGMDPSKMGHALEPGRVTFDEKMDLR 635
MS L Q VEAIK+AGN F+PSEFGMDP+ M HALEPGRVTFDEKM +R
Sbjct: 302 MSGVHFRSHNLMLQLKLVEAIKDAGNVKRFLPSEFGMDPALMEHALEPGRVTFDEKMTIR 481
Query: 634 RAIEDANIPHTYVSANCFA 578
+ IEDANIP TY+SANCFA
Sbjct: 482 KTIEDANIPFTYISANCFA 538
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,452,261
Number of Sequences: 7669
Number of extensions: 116222
Number of successful extensions: 2398
Number of sequences better than 1.0e-020: 7
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 211235160
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)