TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3742548.2.1
(554 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 93 5e-031
gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular tr... 93 5e-031
gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 93 2e-030
gb|CO513758.1|CO513758 s13dSG15G1200088_140298 Glandular tr... 103 2e-023
gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular tr... 96 4e-021
>gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-K1) mRNA, complete cds
Length = 1904
Score = 93.2 bits (197), Expect(2) = 5e-031
Identities = 37/80 (46%), Positives = 47/80 (58%)
Frame = -1 / +1
Query: 410 DFKGADFEFIPFGAGRRICPGMTFGXXXXXXXXXXXXXXFDWSLPGGLTAEELDMTEAFG 231
DFKG++FEFIPFG+GRR CPGM G F W LP G+ E+DM++ FG
Sbjct: 1402 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 1581
Query: 230 IATPRRSDLLVVATPRVPLP 171
+ PR S L+ + T RV P
Sbjct: 1582 LTAPRASRLIAIPTKRVLCP 1641
Score = 55.1 bits (114), Expect(2) = 5e-031
Identities = 21/37 (56%), Positives = 24/37 (64%)
Frame = -1 / +1
Query: 536 VLGYDVPAGITVIVNAWAIGRDPAPWDEPXKFLPERF 426
V GY +P V++NAWAIGRD WDEP F P RF
Sbjct: 1273 VNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRF 1383
>gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 93.2 bits (197), Expect(2) = 5e-031
Identities = 37/80 (46%), Positives = 47/80 (58%)
Frame = -1 / +2
Query: 410 DFKGADFEFIPFGAGRRICPGMTFGXXXXXXXXXXXXXXFDWSLPGGLTAEELDMTEAFG 231
DFKG++FEFIPFG+GRR CPGM G F W LP G+ E+DM++ FG
Sbjct: 248 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 427
Query: 230 IATPRRSDLLVVATPRVPLP 171
+ PR S L+ + T RV P
Sbjct: 428 LTAPRASRLIAIPTKRVLCP 487
Score = 55.1 bits (114), Expect(2) = 5e-031
Identities = 21/37 (56%), Positives = 24/37 (64%)
Frame = -1 / +2
Query: 536 VLGYDVPAGITVIVNAWAIGRDPAPWDEPXKFLPERF 426
V GY +P V++NAWAIGRD WDEP F P RF
Sbjct: 119 VNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRF 229
>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
Length = 1885
Score = 93.2 bits (197), Expect(2) = 2e-030
Identities = 37/80 (46%), Positives = 47/80 (58%)
Frame = -1 / +2
Query: 410 DFKGADFEFIPFGAGRRICPGMTFGXXXXXXXXXXXXXXFDWSLPGGLTAEELDMTEAFG 231
DFKG++FEFIPFG+GRR CPGM G F W LP G+ E+DM++ FG
Sbjct: 1388 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 1567
Query: 230 IATPRRSDLLVVATPRVPLP 171
+ PR S L+ + T RV P
Sbjct: 1568 LTAPRASRLIAIPTKRVLCP 1627
Score = 53.3 bits (110), Expect(2) = 2e-030
Identities = 20/37 (54%), Positives = 24/37 (64%)
Frame = -1 / +2
Query: 536 VLGYDVPAGITVIVNAWAIGRDPAPWDEPXKFLPERF 426
V GY +P V++NAWAIGRD W+EP F P RF
Sbjct: 1259 VNGYFIPKQARVMINAWAIGRDANCWEEPESFKPSRF 1369
>gb|CO513758.1|CO513758 s13dSG15G1200088_140298 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 473
Score = 103 bits (219), Expect = 2e-023
Identities = 44/97 (45%), Positives = 55/97 (56%)
Frame = -1 / +1
Query: 482 IGRDPAPWDEPXKFLPERFEQSTRDFKGADFEFIPFGAGRRICPGMTFGXXXXXXXXXXX 303
+GRDPA WD P F+PERF + ++KG +FE IPFGAGRRICPG+
Sbjct: 4 MGRDPAIWDNPNPFVPERFMECDINYKGNNFELIPFGAGRRICPGLPLAHRTMHLMVASL 183
Query: 302 XXXFDWSLPGGLTAEELDMTEAFGIATPRRSDLLVVA 192
F+W+L GL E L+M E FG+ R L V A
Sbjct: 184 LHNFEWNLADGLIPEHLNMDEQFGLTLKRVQPLRVEA 294
>gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 506
Score = 95.9 bits (203), Expect = 4e-021
Identities = 37/58 (63%), Positives = 42/58 (72%)
Frame = -1 / +2
Query: 542 CQVLGYDVPAGITVIVNAWAIGRDPAPWDEPXKFLPERFEQSTRDFKGADFEFIPFGA 369
CQ+ GYD+PA VIVNAWAIGRDP W + F PERF S DFKG DFE+IPFG+
Sbjct: 332 CQINGYDIPAKTRVIVNAWAIGRDPRYWVDAESFKPERFLNSRFDFKGTDFEYIPFGS 505
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,189,204
Number of Sequences: 7669
Number of extensions: 52150
Number of successful extensions: 1300
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 133581877
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)