TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3742446.2.1
         (598 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CO515781.1|CO515781  s13dSG77H0100016_419711 Glandular tr...    78   5e-025
gb|DR159680.1|DR159680  EST34 Medicago sativa cDNA-AFLP Medi...    74   4e-024

>gb|CO515781.1|CO515781 s13dSG77H0100016_419711 Glandular trichomes Medicago sativa cDNA,
           mRNA sequence
          Length = 550

 Score = 77.6 bits (163), Expect(3) = 5e-025
 Identities = 28/54 (51%), Positives = 35/54 (64%)
 Frame = +2 / +2

                                                                 
Query: 62  GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
           GWLS ++++ F +YA+ CF  FGDRVK W T NEP   A   YD G+  P RCS
Sbjct: 110 GWLSREVIKDFTNYADVCFREFGDRVKYWTTVNEPNIFAVGSYDQGISPPRRCS 271




 Score = 35.0 bits (70), Expect(3) = 5e-025
 Identities = 10/18 (55%), Positives = 15/18 (83%)
 Frame = +2 / +3

                             
Query: 431 FHLGWFLDPIVHGRYPYS 484
           F+LGW ++P++HG YP S
Sbjct: 495 FYLGWIMEPLLHGDYPDS 548




 Score = 33.1 bits (66), Expect(3) = 5e-025
 Identities = 15/39 (38%), Positives = 17/39 (43%)
 Frame = +2 / +2

                                                  
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGI 355
           GNST EPY              R YR  Y+  Q G +GI
Sbjct: 302 GNSTFEPYLVVHHILLAHSSAVRLYRRTYREEQNGFVGI 418



>gb|DR159680.1|DR159680 EST34 Medicago sativa cDNA-AFLP Medicago sativa cDNA clone S15 5',
           mRNA sequence
          Length = 352

 Score = 73.9 bits (155), Expect(2) = 4e-024
 Identities = 29/49 (59%), Positives = 33/49 (67%)
 Frame = +2 / +3

                                                            
Query: 77  KIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
           +IV+ F DYAE CF  FGDRVK+W T NEP  V+   Y  G  APGRCS
Sbjct: 21  RIVDDFRDYAELCFKEFGDRVKHWITLNEPWSVSMNAYAYGKFAPGRCS 167




 Score = 51.0 bits (105), Expect(2) = 4e-024
 Identities = 22/53 (41%), Positives = 26/53 (49%)
 Frame = +2 / +3

                                                                
Query: 236 GGNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDS 394
           GG+S  EPY              + YR KYQ  Q GKIGI L   W+EP S +
Sbjct: 192 GGDSGAEPYLAAHYQLLAHAAAVKLYRTKYQASQNGKIGITLLSHWFEPASQA 350



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 5,664,785
Number of Sequences: 7669
Number of extensions: 80163
Number of successful extensions: 1622
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 146659823
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)