TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3276111.2.1
(1393 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO515300.1|CO515300 s13dSG48C1200098_417659 Glandular tr... 68 7e-028
gb|CO512362.1|CO512362 s13dSG80A0800053_114238 Glandular tr... 63 9e-022
emb|AJ245868.1|MSA245868 Medicago sativa mRNA for cysteine ... 56 1e-021
>gb|CO515300.1|CO515300 s13dSG48C1200098_417659 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 588
Score = 67.5 bits (141), Expect(5) = 7e-028
Identities = 25/37 (67%), Positives = 28/37 (75%)
Frame = +1 / +2
Query: 484 SCSSCWAFVTAATIESITKITTGKLVSLSEQELIDCD 594
SC SCWAF A +E I KI TG L+SLSEQEL+DCD
Sbjct: 356 SCGSCWAFSAIAAVEGINKIVTGDLISLSEQELVDCD 466
Score = 36.3 bits (73), Expect(5) = 7e-028
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +1 / +2
Query: 598 YDGGCNLGYFVNGYRWVIQNGGLTTEANYP 687
Y+ GCN G + ++I NGG+ +E +YP
Sbjct: 473 YNEGCNGGLMDYAFEFIISNGGIDSEEDYP 562
Score = 30.9 bits (61), Expect(5) = 7e-028
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +1 / +2
Query: 274 NLTYTLGENQFADLTEEEF 330
N TY LG +FADLT EE+
Sbjct: 140 NSTYRLGLTRFADLTNEEY 196
Score = 30.9 bits (61), Expect(5) = 7e-028
Identities = 9/33 (27%), Positives = 20/33 (60%)
Frame = +1 / +2
Query: 166 WQATYNRSYPTAEERQRRFQVYRRNIEHIEATN 264
W + +SY E+ +RF++++ N++ I+ N
Sbjct: 35 WLVKHGKSYNGLGEKDKRFEIFKDNLKFIDEHN 133
Score = 25.4 bits (49), Expect(5) = 7e-028
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +1 / +2
Query: 436 DWRSKGAVTPIKNQ 477
DWR +GAV +K+Q
Sbjct: 311 DWRKEGAVVGVKDQ 352
>gb|CO512362.1|CO512362 s13dSG80A0800053_114238 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 576
Score = 63.4 bits (132), Expect(3) = 9e-022
Identities = 23/37 (62%), Positives = 27/37 (72%)
Frame = +1 / +1
Query: 484 SCSSCWAFVTAATIESITKITTGKLVSLSEQELIDCD 594
SC SCWAF T +E + TGKLVSLSEQ+L+DCD
Sbjct: 337 SCGSCWAFSTTGALEGANYLATGKLVSLSEQQLVDCD 447
Score = 36.3 bits (73), Expect(3) = 9e-022
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +1 / +1
Query: 601 DGGCNLGYFVNGYRWVIQNGGLTTEANY 684
D GCN G N + +++Q+GG+ +E +Y
Sbjct: 481 DSGCNGGLMNNAFEYILQSGGVVSEKDY 564
Score = 36.3 bits (73), Expect(3) = 9e-022
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +1 / +1
Query: 436 DWRSKGAVTPIKNQG 480
DWR KGAVTP+K+QG
Sbjct: 292 DWREKGAVTPVKDQG 336
>emb|AJ245868.1|MSA245868 Medicago sativa mRNA for cysteine protease, partial (cyp15a gene)
Length = 890
Score = 56.1 bits (116), Expect(3) = 1e-021
Identities = 21/36 (58%), Positives = 25/36 (69%)
Frame = +1 / +1
Query: 487 CSSCWAFVTAATIESITKITTGKLVSLSEQELIDCD 594
C S WAF T +E + TGKLVSLSEQ+L+DCD
Sbjct: 1 CGSGWAFSTTGALEGANYLATGKLVSLSEQQLVDCD 108
Score = 49.2 bits (101), Expect(3) = 1e-021
Identities = 16/33 (48%), Positives = 21/33 (63%)
Frame = +1 / +1
Query: 913 GYGADSSSGLKYWLVKNSWGQSWGERGYLRMRR 1011
GY YW++KNSWGQ+WGE GY ++ R
Sbjct: 472 GYAPIRLKEKPYWIIKNSWGQNWGEEGYYKICR 570
Score = 36.3 bits (73), Expect(3) = 1e-021
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +1 / +1
Query: 601 DGGCNLGYFVNGYRWVIQNGGLTTEANY 684
D GCN G N + +++Q+GG+ +E +Y
Sbjct: 142 DSGCNGGLMNNAFEYILQSGGVVSEKDY 225
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,280,623
Number of Sequences: 7669
Number of extensions: 77987
Number of successful extensions: 498
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 381764308
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)