TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3204398.2.1
(666 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO517275.1|CO517275 s13dSG32H1100096_486146 Glandular tr... 166 3e-042
emb|Y11348.1|MSNANN M.sativa mRNA for annexin-like protein 155 2e-039
>gb|CO517275.1|CO517275 s13dSG32H1100096_486146 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 628
Score = 166 bits (357), Expect = 3e-042
Identities = 68/93 (73%), Positives = 81/93 (87%)
Frame = +1 / -1
Query: 1 TSGNFEFALLAILRCAENPAKYFAKLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEY 180
TSGNF ALL I++CAE+PAKYFAK+LR+AMKGLGTDD L+RV+VTR+EID+ YIKAEY
Sbjct: 601 TSGNFAHALLTIVQCAESPAKYFAKVLRRAMKGLGTDDTKLMRVIVTRSEIDLHYIKAEY 422
Query: 181 FKKYKKPLAEAIHSETSGNYRTFLLSLVGPGH* 279
KKYKK L +A+HSETSG+YR FLLSL+GP *
Sbjct: 421 LKKYKKTLNDAVHSETSGHYRAFLLSLLGPNQ* 323
Score = 133 bits (286), Expect = 2e-032
Identities = 61/89 (68%), Positives = 69/89 (77%)
Frame = -2 / +3
Query: 269 GPTSERRNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISVLSSVPRPFM 90
GP SERR *+PDVSE AS + FLYF KYSA I C SIS+LVT TLIS +SSVPRPF+
Sbjct: 333 GPKSERRKAL*WPDVSE*TASFSVFLYFFKYSAFI*CRSISLLVTITLISFVSSVPRPFI 512
Query: 89 AFRKSFAKYFAGFSAHLRIAKSANSKFPD 3
A R++FAKYFAG SAH I KSA +KFPD
Sbjct: 513 ALRRTFAKYFAGLSAHWTIVKSA*AKFPD 599
>emb|Y11348.1|MSNANN M.sativa mRNA for annexin-like protein
Length = 1533
Score = 155 bits (332), Expect(2) = 2e-039
Identities = 65/82 (79%), Positives = 70/82 (85%)
Frame = +1 / +2
Query: 4 SGNFEFALLAILRCAENPAKYFAKLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEYF 183
SGNF ALL I CA NPAKYFAK+L KAMKGLGT+D TLIRV+VTRTEIDMQYIKAEY
Sbjct: 785 SGNFGLALLTITECATNPAKYFAKVLYKAMKGLGTNDSTLIRVIVTRTEIDMQYIKAEYA 964
Query: 184 KKYKKPLAEAIHSETSGNYRTF 249
KKYKK L +A+HSETSGNYR F
Sbjct: 965 KKYKKTLNDAVHSETSGNYRIF 1030
Score = 121 bits (259), Expect = 8e-029
Identities = 55/83 (66%), Positives = 62/83 (74%)
Frame = -2 / -1
Query: 251 RNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISVLSSVPRPFMAFRKSF 72
+ + * PDVSE AS + FLYF YSALIYCISISVLVT TL+SVLS VP+PF+A +F
Sbjct: 1032 KKIL*LPDVSE*TASFSVFLYFFAYSALIYCISISVLVTMTLMSVLSLVPKPFIALYNTF 853
Query: 71 AKYFAGFSAHLRIAKSANSKFPD 3
AKYFAG AH I KSA KFPD
Sbjct: 852 AKYFAGLVAHSVIVKSARPKFPD 784
Score = 22.1 bits (42), Expect(2) = 2e-039
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = +1 / +1
Query: 247 FLLSLVGPGH* 279
FLL+L+GP H*
Sbjct: 1027 FLLALLGPNH* 1059
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,970,373
Number of Sequences: 7669
Number of extensions: 83041
Number of successful extensions: 1059
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 165838130
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)