TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3199064.2.1
(589 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF049487.1|AF049487 Medicago sativa sucrose synthase mRN... 177 8e-046
gb|CO515699.1|CO515699 s13dSG60G0600052_419547 Glandular tr... 108 5e-025
gb|CO514282.1|CO514282 s13dSG76F0500043_157226 Glandular tr... 108 7e-025
>gb|AF049487.1|AF049487 Medicago sativa sucrose synthase mRNA, complete cds
Length = 2760
Score = 177 bits (382), Expect = 8e-046
Identities = 78/114 (68%), Positives = 90/114 (78%)
Frame = +3 / +2
Query: 3 RVVHGIDVFDPKFNIVSPGADMSIYFPHTEKAKRLTSLHGSIENLIYDPEQNDEHIGHLD 182
RVVHGIDVFDPKFNIVSPGAD +IYFP+TE ++RLTS + IE L+Y +N+EHI L
Sbjct: 1580 RVVHGIDVFDPKFNIVSPGADQTIYFPYTETSRRLTSFYPEIEELLYSSVENEEHICVLK 1759
Query: 183 DRSKPILFSMARLDRVKNITGLVEAFAKCAKLRELVNLVVVATHGHSASGIYEE 344
DR+KPI+F+MARLDRVKNITGLVE + K AKLRELVNLVVVA S EE
Sbjct: 1760 DRNKPIIFTMARLDRVKNITGLVEWYGKNAKLRELVNLVVVAGDRRKESKDLEE 1921
Score = 103 bits (220), Expect = 2e-023
Identities = 56/102 (54%), Positives = 64/102 (62%)
Frame = -1 / -1
Query: 307 ATTTRFTXXXXXXXXXXXXXSPVMFFTLSSLAMEKRMGFDRSSRCPMCSSFCSGS*IKFS 128
ATTTRFT SPVMFFT S+LAM K +G RS MCSSF + * S
Sbjct: 1884 ATTTRFTNSRSLAFLPYHSTSPVMFFTRSNLAMVKIIGLLRSFSTHMCSSFSTEL*RSSS 1705
Query: 127 IEP*REVSRLAFSVCGKYMDMSAPGETILNFGSKTSIPWTTR 2
I *++V+R SV GK M SAPG+T+LNFGSKTSIP TTR
Sbjct: 1704 ISG*KDVNRRLVSV*GK*MV*SAPGDTMLNFGSKTSIPCTTR 1579
>gb|CO515699.1|CO515699 s13dSG60G0600052_419547 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 449
Score = 108 bits (231), Expect = 5e-025
Identities = 51/81 (62%), Positives = 58/81 (71%)
Frame = +3 / +3
Query: 102 RLTSLHGSIENLIYDPEQNDEHIGHLDDRSKPILFSMARLDRVKNITGLVEAFAKCAKLR 281
RL S H IE L+Y +N+EHI L DRSKPI+F+MARLDRVKNITGLVE + K A+LR
Sbjct: 6 RLKSFHPEIEELLYSSVENEEHICVLKDRSKPIIFTMARLDRVKNITGLVEWYGKNARLR 185
Query: 282 ELVNLVVVATHGHSASGIYEE 344
ELVNLVVVA S EE
Sbjct: 186 ELVNLVVVAGDRRKESKDLEE 248
>gb|CO514282.1|CO514282 s13dSG76F0500043_157226 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 515
Score = 108 bits (230), Expect = 7e-025
Identities = 47/60 (78%), Positives = 53/60 (88%)
Frame = +3 / +1
Query: 129 ENLIYDPEQNDEHIGHLDDRSKPILFSMARLDRVKNITGLVEAFAKCAKLRELVNLVVVA 308
E L+YD EQ DE IG L DRSKPI+FSMARLDRVKNITGLVE++AK +KLRELVNL+VVA
Sbjct: 4 EKLLYDTEQTDECIGSLADRSKPIIFSMARLDRVKNITGLVESYAKNSKLRELVNLIVVA 183
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,318,040
Number of Sequences: 7669
Number of extensions: 69690
Number of successful extensions: 1349
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 143857406
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)