TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3198833.2.1
(593 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U28148.1|MSU28148 Medicago sativa putative pectinesteras... 149 1e-061
gb|CO514932.1|CO514932 s13dSG63B1100093_397369 Glandular tr... 201 7e-053
gb|AY587268.1| Medicago sativa pectin methylesterase (PEF) ... 135 7e-035
gb|AY587267.1| Medicago sativa pectin methylesterase (PER) ... 133 8e-034
gb|CO514488.1|CO514488 s13dSG43C1100084_327636 Glandular tr... 110 1e-030
>gb|U28148.1|MSU28148 Medicago sativa putative pectinesterase mRNA, complete cds
Length = 1573
Score = 149 bits (319), Expect(2) = 1e-061
Identities = 63/112 (56%), Positives = 74/112 (66%)
Frame = +1 / +1
Query: 1 MGFHNTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIF 180
+GF NTAG +HQAVALRV D A FYNC+ D FQDTLYV ++RQF+R+C ISGTIDF+F
Sbjct: 748 IGFENTAGTGKHQAVALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVF 927
Query: 181 GNSAAVFQNCLIVTRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKL 336
G VFQNC +V R P QQ VTA GR N S LV Q+ + L
Sbjct: 928 GERFGVFQNCKLVCRLPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPAL 1083
Score = 101 bits (216), Expect(2) = 1e-061
Identities = 40/75 (53%), Positives = 51/75 (68%)
Frame = +1 / +1
Query: 361 SYLGRPWKEFSRLVIMESTIADFIKPEGYMPWNGDFGIKTLYYAEYNNRGPGAGTSKRVT 540
SYLGRPWK++S++VIM+STI PEGYMPW G +T Y EYNN+GPGA T+ RV
Sbjct: 1105 SYLGRPWKQYSKVVIMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVK 1284
Query: 541 WPGFHVIGRKDAEQF 585
W G V+ A ++
Sbjct: 1285 WHGVKVLTSNVAAEY 1329
Score = 89.0 bits (188), Expect(2) = 4e-032
Identities = 46/103 (44%), Positives = 49/103 (47%)
Frame = -1 / -3
Query: 311 QFWMTSPDFMLGSVRPCAVTEFCWLSMGRRVTMRQFWNTAAELPKMKSMVPEMTQLRKNW 132
+ W T + RP AVT CW G T QFW T PK KS+VPEM Q R NW
Sbjct: 1058 ELWKTRALALFCFSRPPAVTRHCWPFAGNLQTSLQFWKTPKRSPKTKSIVPEMLQSR*NW 879
Query: 131 RRACTYSVSWNASNRQL*NXXXXXXXXXXXAWCLSAPAVLWKP 3
R T SVSW S QL*N A CL PAV KP
Sbjct: 878 RWDWT*SVSWKPSI*QL*NIAWSAVTRRATA*CLPVPAVFSKP 750
Score = 62.9 bits (131), Expect(2) = 4e-032
Identities = 33/71 (46%), Positives = 41/71 (57%)
Frame = -1 / -3
Query: 578 SASFRPMTWNPGQVTLLLVPAPGPRLLYSA**SVLMPKSPFQGM*PSGLMKSAMVLSMMT 399
+A+F T P +TL V APGP LLYS * V + P GM*PSG ++MV S++T
Sbjct: 1322 AATFEVKTLTPCHLTLKFVSAPGPLLLYS**VHVSLKALPIHGM*PSGTKIASMVESIIT 1143
Query: 398 RRENSFQGRPR 366
E F G PR
Sbjct: 1142 TLEYCFHGLPR 1110
>gb|CO514932.1|CO514932 s13dSG63B1100093_397369 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 461
Score = 201 bits (433), Expect = 7e-053
Identities = 78/152 (51%), Positives = 100/152 (65%)
Frame = +1 / +2
Query: 34 HQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVFQNCL 213
HQAVALRV D A Y C +QDT+Y H+ RQF+R C I GT+DFIFGN+A VFQNC
Sbjct: 5 HQAVALRVGSDHAVVYRCNVIGYQDTMYAHSNRQFYRECDIYGTVDFIFGNAAVVFQNCS 184
Query: 214 IVTRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLFPDRFKIPSYLGRPWKEFS 393
+ R+PM Q+N++TA R DPN +G+ I NCR++ Q L + +YLGRPWK +S
Sbjct: 185 LYARKPMPYQKNTITAQNRKDPNQNTGISIHNCRILATQDLEASKGNFTTYLGRPWKLYS 364
Query: 394 RLVIMESTIADFIKPEGYMPWNGDFGIKTLYY 489
R V M S + D I P G++ WN F + TLYY
Sbjct: 365 RTVYMLSYMGDHIHPRGWLEWNTTFALDTLYY 460
>gb|AY587268.1| Medicago sativa pectin methylesterase (PEF) mRNA, partial cds
Length = 351
Score = 135 bits (290), Expect(2) = 7e-035
Identities = 57/102 (55%), Positives = 69/102 (67%)
Frame = +1 / +1
Query: 31 RHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVFQNC 210
+HQAVALRV D A FYNC+ D FQDTLYV ++RQF+R+C ISGTIDF+FG++ VFQNC
Sbjct: 1 KHQAVALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGDAFGVFQNC 180
Query: 211 LIVTRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKL 336
+V R P QQ VTA GR N S LV Q+ + L
Sbjct: 181 KLVCRLPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPAL 306
Score = 25.4 bits (49), Expect(2) = 7e-035
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1 / +1
Query: 361 SYLGRPWK 384
SYLGRPWK
Sbjct: 328 SYLGRPWK 351
>gb|AY587267.1| Medicago sativa pectin methylesterase (PER) mRNA, partial cds
Length = 351
Score = 133 bits (285), Expect(2) = 8e-034
Identities = 50/102 (49%), Positives = 72/102 (70%)
Frame = +1 / +1
Query: 31 RHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVFQNC 210
+HQAVALRV D A FYNC+ D +Q TL+ ++RQF+R+C ISGTID ++G++ AVFQNC
Sbjct: 1 KHQAVALRVTADKAIFYNCQIDGYQATLFAESQRQFYRDCSISGTIDMVYGDAFAVFQNC 180
Query: 211 LIVTRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKL 336
++ R+P++ QQ V A GRT + SG V Q+C + ++
Sbjct: 181 KLIVRKPLEEQQCFVAADGRTKSDSSSGFVFQSCHFTGEPEV 306
Score = 24.0 bits (46), Expect(2) = 8e-034
Identities = 7/8 (87%), Positives = 8/8 (100%)
Frame = +1 / +1
Query: 361 SYLGRPWK 384
+YLGRPWK
Sbjct: 328 AYLGRPWK 351
>gb|CO514488.1|CO514488 s13dSG43C1100084_327636 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 592
Score = 110 bits (235), Expect(2) = 1e-030
Identities = 38/69 (55%), Positives = 53/69 (76%)
Frame = +1 / +3
Query: 355 IPSYLGRPWKEFSRLVIMESTIADFIKPEGYMPWNGDFGIKTLYYAEYNNRGPGAGTSKR 534
+ S+LGRPW+++SR V M++ I FI P G++PW+G+F + TLYYAEY N G G+ TS R
Sbjct: 93 VKSHLGRPWQKYSRTVFMKTVIDGFINPAGWLPWDGNFALDTLYYAEYANTGSGSSTSNR 272
Query: 535 VTWPGFHVI 561
VTW G+HV+
Sbjct: 273 VTWKGYHVL 299
Score = 36.3 bits (73), Expect(2) = 1e-030
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +1 / +3
Query: 259 AHGRTDPNMKSGLVIQNCRLVPDQKLFPDRFK 354
A GRTDPN +G++I N R+ L P K
Sbjct: 3 AQGRTDPNQNTGIIIHNSRVSAQSDLNPSSVK 98
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,313,498
Number of Sequences: 7669
Number of extensions: 38478
Number of successful extensions: 966
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 145725684
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)