TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3185308.2.1
(1019 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514640.1|CO514640 s13dSG46C0600050_327940 Glandular tr... 87 4e-035
gb|CO513205.1|CO513205 s13dSG24B0800061_129574 Glandular tr... 79 1e-030
>gb|CO514640.1|CO514640 s13dSG46C0600050_327940 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 536
Score = 86.8 bits (183), Expect(4) = 4e-035
Identities = 33/56 (58%), Positives = 45/56 (80%)
Frame = +3 / +3
Query: 426 HFDLTIPAFLKIAEEKAGIVPITYRKVACERKGGIRYTITGNQHYSEVKVTNVGGA 593
HFDL+ P F IA+ +AGIVP+ YR+V C+R+GGIR+TI G+ +++ V VTNVGGA
Sbjct: 369 HFDLSQPIFQHIAQYRAGIVPVAYRRVRCKRRGGIRFTINGHSYFNLVLVTNVGGA 536
Score = 58.8 bits (122), Expect(4) = 4e-035
Identities = 22/41 (53%), Positives = 27/41 (65%)
Frame = +3 / +3
Query: 231 TDSGACGYKGELGKDYGTLTAAVGPSLYTNGTGCGACYELK 353
T GACGY + YGT TAA+ +L+ NG CGACYE+K
Sbjct: 150 TMGGACGYGNLYSQGYGTNTAALSTALFNNGLSCGACYEIK 272
Score = 28.1 bits (55), Expect(4) = 4e-035
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = +3 / +3
Query: 177 WTDGHATYYGGRD 215
W + HAT+YGG D
Sbjct: 102 WINAHATFYGGSD 140
Score = 24.9 bits (48), Expect(4) = 4e-035
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = +3 / +3
Query: 363 GTVVVTATNEAPP 401
G+++VTATN PP
Sbjct: 303 GSIIVTATNFCPP 341
>gb|CO513205.1|CO513205 s13dSG24B0800061_129574 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 592
Score = 79.0 bits (166), Expect(4) = 1e-030
Identities = 29/51 (56%), Positives = 41/51 (80%)
Frame = +3 / +2
Query: 423 EHFDLTIPAFLKIAEEKAGIVPITYRKVACERKGGIRYTITGNQHYSEVKV 575
+HFDL P FL+IA+ KAGIVP++YR+V C R+GGIR+TI G+ +++ V V
Sbjct: 440 QHFDLAQPVFLRIAQYKAGIVPVSYRRVPCRRRGGIRFTINGHSYFNLVLV 592
Score = 51.9 bits (107), Expect(4) = 1e-030
Identities = 18/41 (43%), Positives = 27/41 (65%)
Frame = +3 / +2
Query: 231 TDSGACGYKGELGKDYGTLTAAVGPSLYTNGTGCGACYELK 353
T GACGY + YGT TAA+ +L+ +G CG+C+E++
Sbjct: 200 TMGGACGYGNLYSQGYGTNTAALSTALFNSGLSCGSCFEIR 322
Score = 28.6 bits (56), Expect(4) = 1e-030
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = +3 / +2
Query: 177 WTDGHATYYGGRD 215
W + HAT+YGG D
Sbjct: 152 WVNAHATFYGGSD 190
Score = 24.0 bits (46), Expect(4) = 1e-030
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = +3 / +2
Query: 363 GTVVVTATNEAPP 401
G+++VTATN PP
Sbjct: 353 GSILVTATNFCPP 391
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,661,406
Number of Sequences: 7669
Number of extensions: 79719
Number of successful extensions: 715
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 271965096
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)