TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115184.2.2
(682 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA... 155 4e-039
emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene 91 7e-032
gb|AY219848.1| Medicago sativa polygalactorunase PG11 precu... 98 9e-022
>gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA, complete cds
Length = 1492
Score = 155 bits (334), Expect = 4e-039
Identities = 60/129 (46%), Positives = 89/129 (68%)
Frame = +1 / +1
Query: 1 GISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRIKSYEDSKSSLKASKFLYEGITMDNV 180
G+SVGSLG++ E++VE + V CT+ T NG+RIK++ D+ ++ S +E ITM NV
Sbjct: 757 GLSVGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMTNV 936
Query: 181 SYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLV 360
P+IIDQ+Y P N C K SK+ ++ + FKN+ GTS T E + L C++ +PC GV+L
Sbjct: 937 KNPVIIDQEYYPWNQCSKKNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVELN 1116
Query: 361 NVDIKYNGS 387
NVD+K+NG+
Sbjct: 1117 NVDLKFNGA 1143
>emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene
Length = 2185
Score = 90.9 bits (192), Expect(2) = 7e-032
Identities = 40/78 (51%), Positives = 50/78 (64%)
Frame = +1 / +1
Query: 1 GISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRIKSYEDSKSSLKASKFLYEGITMDNV 180
GISVGSLG+Y E+ VE V V CTI T NG+RIK++ S + + +E I M NV
Sbjct: 1471 GISVGSLGKYPKEEAVEHVLVKNCTITNTDNGVRIKTWPSSPGTSPITDMHFEDIIMVNV 1650
Query: 181 SYPIIIDQKYCPNNICVK 234
P+IIDQ+YCP N C K
Sbjct: 1651 LNPVIIDQEYCPWNQCSK 1704
Score = 60.6 bits (126), Expect(2) = 7e-032
Identities = 21/48 (43%), Positives = 33/48 (68%)
Frame = +1 / +2
Query: 244 SKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIKYNGS 387
SK+ ++ V+FKNI GTS T + + L C+ + PC V+L NV + +NG+
Sbjct: 1808 SKIKISKVIFKNIRGTSKTKDGVVLICSKSFPCDAVELNNVALTFNGA 1951
>gb|AY219848.1| Medicago sativa polygalactorunase PG11 precursor, gene, complete cds
Length = 2213
Score = 98.2 bits (208), Expect = 9e-022
Identities = 41/91 (45%), Positives = 59/91 (64%)
Frame = +1 / +1
Query: 1 GISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRIKSYEDSKSSLKASKFLYEGITMDNV 180
GISVGSLG+Y E +VE V V CT+ T NG+RIK++ D+ ++ S +E I M+NV
Sbjct: 1492 GISVGSLGKYTTEDNVEGVIVKNCTLTATQNGVRIKTWPDAPGTITVSDIHFEDIIMNNV 1671
Query: 181 SYPIIIDQKYCPNNICVKSGASKVAVNDVVF 273
P+IIDQ+YCP N C K ++ ++ V+
Sbjct: 1672 MNPVIIDQEYCPWNQCSKKVITQELISCFVY 1764
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,009,414
Number of Sequences: 7669
Number of extensions: 67323
Number of successful extensions: 999
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 170470480
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)