TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115139.2.1
(1079 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular tr... 132 1e-046
gb|CO512956.1|CO512956 s13dSG86G1100084_121838 Glandular tr... 132 7e-046
emb|X80222.1|MSDFR1 M.sativa mRNA for dihydroflavonol-4-red... 99 3e-040
gb|U28213.1|MSU28213 Medicago sativa vestitone reductase mR... 57 4e-040
gb|CO515075.1|CO515075 s13dSG97E0800067_397655 Glandular tr... 100 2e-022
>gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 602
Score = 132 bits (282), Expect(3) = 1e-046
Identities = 56/80 (70%), Positives = 62/80 (77%)
Frame = +1 / +1
Query: 25 VCVTGASGFIASWLVKRLLESGYHVLGTVRDPGNGKKVGHLWGLEGAKERLQLVRADLLE 204
VCVTGASG+IASWLV+ LL GY V TVRDP + KK HL LEGAKERLQL +A+LLE
Sbjct: 70 VCVTGASGYIASWLVRLLLNRGYTVKATVRDPNDPKKGDHLVKLEGAKERLQLFKANLLE 249
Query: 205 EGSFDDAVMACEGVFHTASP 264
EG+FD V C GVFHTASP
Sbjct: 250 EGAFDSVVQGCHGVFHTASP 309
Score = 52.4 bits (108), Expect(3) = 1e-046
Identities = 18/44 (40%), Positives = 30/44 (68%)
Frame = +1 / +1
Query: 424 PNVLLDESSWSSIEFCESLQIWYAVAKILAEKAAWEFAGEHRID 555
P+V++DE+ ++ + C +WY V+K LAE+AAW+F + ID
Sbjct: 469 PDVVVDETWFTDADLCAKSNLWYVVSKTLAEEAAWKFVKVNNID 600
Score = 35.9 bits (72), Expect(3) = 1e-046
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = +1 / +1
Query: 292 EMLDSAINGTMNVLRSCKKNP 354
E++D A+ GT+NVL SC K+P
Sbjct: 340 ELIDPALKGTLNVLNSCAKSP 402
>gb|CO512956.1|CO512956 s13dSG86G1100084_121838 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 584
Score = 132 bits (283), Expect(3) = 7e-046
Identities = 57/80 (71%), Positives = 62/80 (77%)
Frame = +1 / +1
Query: 25 VCVTGASGFIASWLVKRLLESGYHVLGTVRDPGNGKKVGHLWGLEGAKERLQLVRADLLE 204
VCVTGASG+IASWLV LL GY V TVRDP + KKV HL LEGAKERLQL +A+LLE
Sbjct: 70 VCVTGASGYIASWLV*LLLNRGYTVKATVRDPNDPKKVDHLVKLEGAKERLQLFKANLLE 249
Query: 205 EGSFDDAVMACEGVFHTASP 264
EG+FD V C GVFHTASP
Sbjct: 250 EGAFDSVVQGCHGVFHTASP 309
Score = 85.8 bits (181), Expect(2) = 3e-021
Identities = 46/80 (57%), Positives = 49/80 (61%)
Frame = -1 / -1
Query: 263 GDAVWKTPSQAITASSKLPSSKRSALTSCSLSFAPSRPQRCPTFLPFPGSLTVPRTWYPD 84
GDAV KTP Q T SK PSS R AL SC+LS APS + TFL GS TV T P
Sbjct: 308 GDAV*KTP*QP*TTESKAPSSSRFALNSCNLSLAPSSLTKWSTFLGSFGSRTVALTV*PR 129
Query: 83 SRSRLTSQEAMKPDAPVTQT 24
RS TS EAM P+APVT T
Sbjct: 128 LRSNQTSHEAM*PEAPVTHT 69
Score = 49.2 bits (101), Expect(3) = 7e-046
Identities = 16/37 (43%), Positives = 27/37 (72%)
Frame = +1 / +1
Query: 424 PNVLLDESSWSSIEFCESLQIWYAVAKILAEKAAWEF 534
P+V++DE+ ++ + C +WY V+K LAE+AAW+F
Sbjct: 469 PDVVVDETWFTDADLCAKSNLWYVVSKTLAEEAAWKF 579
Score = 35.9 bits (72), Expect(3) = 7e-046
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = +1 / +1
Query: 292 EMLDSAINGTMNVLRSCKKNP 354
E++D A+ GT+NVL SC K+P
Sbjct: 340 ELIDPALKGTLNVLNSCAKSP 402
Score = 30.9 bits (61), Expect(2) = 3e-021
Identities = 15/28 (53%), Positives = 16/28 (57%)
Frame = -1 / -1
Query: 383 EDVRTTLLSDGFFLHERSTFIVPFIAES 300
EDV+T LSDG HE T VP A S
Sbjct: 431 EDVKTRRLSDGDLAHELRTLRVPLRAGS 348
>emb|X80222.1|MSDFR1 M.sativa mRNA for dihydroflavonol-4-reductase
Length = 650
Score = 99.1 bits (210), Expect(3) = 3e-040
Identities = 44/78 (56%), Positives = 52/78 (66%)
Frame = +1 / +1
Query: 58 SWLVKRLLESGYHVLGTVRDPGNGKKVGHLWGLEGAKERLQLVRADLLEEGSFDDAVMAC 237
SWLV RL+E GY V TVRDP N KKV L L GAK +L + +ADL EEGSFD+A+ C
Sbjct: 4 SWLVMRLMEPGYMVRATVRDPENLKKVSPLLELPGAKSKLSIWKADLGEEGSFDEAIKGC 183
Query: 238 EGVFHTASPVVTGSNSKE 291
GVFH A+P+ S E
Sbjct: 184 TGVFHVATPMDFESKDPE 237
Score = 74.8 bits (157), Expect(3) = 3e-040
Identities = 29/71 (40%), Positives = 44/71 (61%)
Frame = +1 / +1
Query: 490 YAVAKILAEKAAWEFAGEHRIDLVTVLPTFVVGPTLSPELGPTASDVLGLFQGETGKFTT 669
Y V+K LAE+ AW+FA EH++D++T++P VVGP L P + P+ L G ++
Sbjct: 436 YFVSKTLAEQEAWKFAKEHKMDVITIIPPLVVGPFLIPTMPPSLITALSPITGNEAHYSI 615
Query: 670 YGRMGYVHIDD 702
+ YVH+DD
Sbjct: 616 IKQGQYVHLDD 648
Score = 28.6 bits (56), Expect(3) = 3e-040
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1 / +1
Query: 433 LLDESSWSSIEFCESLQI 486
L DES WS +EFC +++
Sbjct: 370 LWDESCWSDVEFCRRVKM 423
>gb|U28213.1|MSU28213 Medicago sativa vestitone reductase mRNA, complete cds
Length = 1237
Score = 57.0 bits (118), Expect(6) = 4e-040
Identities = 20/33 (60%), Positives = 27/33 (81%)
Frame = +1 / +2
Query: 16 KGKVCVTGASGFIASWLVKRLLESGYHVLGTVR 114
KG+VCVTG +GF+ SW++K LLE+GY V T+R
Sbjct: 65 KGRVCVTGGTGFLGSWIIKSLLENGYSVNTTIR 163
Score = 56.5 bits (117), Expect(6) = 4e-040
Identities = 25/62 (40%), Positives = 33/62 (53%)
Frame = +1 / +2
Query: 115 DPGNGKKVGHLWGLEGAKERLQLVRADLLEEGSFDDAVMACEGVFHTASPVVTGSNSKEE 294
DP + V L L GA E+L ADL SF A+ C G+FHTASP+ + EE
Sbjct: 167 DPERKRDVSFLTNLPGASEKLHFFNADLSNPDSFAAAIEGCVGIFHTASPIDFAVSEPEE 346
Query: 295 ML 300
++
Sbjct: 347 IV 352
Score = 50.1 bits (103), Expect(6) = 4e-040
Identities = 19/52 (36%), Positives = 31/52 (59%)
Frame = +1 / +2
Query: 676 RMGYVHIDDVARCHMLAYEAAGARGRYICSAAVLDCGDLAALLARRFPAYPV 831
R VH+DDVAR H+ E + GRY CS ++ +++ LL+ ++P Y +
Sbjct: 725 RFHMVHVDDVARAHIYLLENSVPGGRYNCSPFIVPIEEMSQLLSAKYPEYQI 880
Score = 49.2 bits (101), Expect(6) = 4e-040
Identities = 23/54 (42%), Positives = 34/54 (62%)
Frame = +1 / +2
Query: 490 YAVAKILAEKAAWEFAGEHRIDLVTVLPTFVVGPTLSPELGPTASDVLGLFQGE 651
YAV+K LAEKA EF ++ ID+VT++ F+VG + P+L + L L G+
Sbjct: 542 YAVSKTLAEKAVLEFGEQNGIDVVTLILPFIVGRFVCPKLPDSIEKALVLVLGK 703
Score = 23.5 bits (45), Expect(6) = 4e-040
Identities = 7/17 (41%), Positives = 12/17 (70%)
Frame = +1 / +2
Query: 433 LLDESSWSSIEFCESLQ 483
+LDES WS ++ S++
Sbjct: 476 VLDESDWSDVDLLRSVK 526
Score = 22.6 bits (43), Expect(6) = 4e-040
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +1 / +2
Query: 922 VEEMFDDAVASFIGHGHLPAAEERRH 999
+E+MFDDA+ G+L A + H
Sbjct: 977 IEDMFDDAIQCCKEKGYL*ACV*KFH 1054
>gb|CO515075.1|CO515075 s13dSG97E0800067_397655 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 346
Score = 100 bits (214), Expect = 2e-022
Identities = 45/65 (69%), Positives = 48/65 (73%)
Frame = +1 / +1
Query: 25 VCVTGASGFIASWLVKRLLESGYHVLGTVRDPGNGKKVGHLWGLEGAKERLQLVRADLLE 204
VCVTGASGFI SWLV RL+E GY V TVRDP N KKV HL L GA +L L +ADL E
Sbjct: 151 VCVTGASGFIGSWLVMRLIEHGYTVRATVRDPDNIKKVKHLLELPGANSKLSLWKADLGE 330
Query: 205 EGSFD 219
EGSFD
Sbjct: 331 EGSFD 345
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,474,882
Number of Sequences: 7669
Number of extensions: 89901
Number of successful extensions: 1037
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 290341116
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)