TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115097.2.2
(1086 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO513205.1|CO513205 s13dSG24B0800061_129574 Glandular tr... 133 1e-056
gb|CO514640.1|CO514640 s13dSG46C0600050_327940 Glandular tr... 120 1e-055
gb|CO514666.1|CO514666 s13dSG46E1100087_327992 Glandular tr... 200 2e-052
gb|CO515743.1|CO515743 s13dSG77C1100086_419635 Glandular tr... 111 2e-045
gb|CO515120.1|CO515120 s13dSG40E0200019_399105 Glandular tr... 61 2e-021
>gb|CO513205.1|CO513205 s13dSG24B0800061_129574 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 592
Score = 133 bits (285), Expect(2) = 1e-056
Identities = 47/79 (59%), Positives = 65/79 (82%)
Frame = +3 / +2
Query: 390 TLVVTATDLCPPNDQQSADSGGWCNPPREHFDLSMPAFLQIAQEKAGIVPISYRRVACTK 569
+++VTAT+ CPPN+ ++GGWC+PP +HFDL+ P FL+IAQ KAGIVP+SYRRV C +
Sbjct: 356 SILVTATNFCPPNNALPNNNGGWCSPPLQHFDLAQPVFLRIAQYKAGIVPVSYRRVPCRR 535
Query: 570 QGGIRYTITGNKYFNMVTI 626
+GGIR+TI G+ YFN+V +
Sbjct: 536 RGGIRFTINGHSYFNLVLV 592
Score = 101 bits (216), Expect(2) = 1e-056
Identities = 37/72 (51%), Positives = 45/72 (62%)
Frame = +3 / +2
Query: 189 AHATFYGGRDGSGTTAGACGYKDTRAQGYGVQTVAVSTVLFGDGAACGGCYEVRCVDSPS 368
AHATFYGG D SGT GACGY + +QGYG T A+ST LF G +CG C+E+RC
Sbjct: 161 AHATFYGGSDASGTMGGACGYGNLYSQGYGTNTAALSTALFNSGLSCGSCFEIRCAGDRK 340
Query: 369 GCKPNAATLVVT 404
C P + + T
Sbjct: 341 WCLPGSILVTAT 376
Score = 76.2 bits (160), Expect(2) = 1e-025
Identities = 37/74 (50%), Positives = 44/74 (59%)
Frame = -3 / -2
Query: 619 TMLKYLFPVMVYRMPPCFVHATLR*EMGTMPAFSCAIWRNAGMLRSKCSRGGLHHPPLSA 440
T LK P++V +PP + TL *E GT+PA AI R G RSKC +GGLHHPPL
Sbjct: 585 TKLK*ECPLIVNLIPPLLLQGTLL*ETGTIPALY*AILRKTG*ARSKCCKGGLHHPPLLL 406
Query: 439 DCWSLGGHRSVAVT 398
GG + VAVT
Sbjct: 405 GKALFGGQKLVAVT 364
Score = 55.1 bits (114), Expect(2) = 1e-025
Identities = 30/64 (46%), Positives = 31/64 (48%)
Frame = -3 / -2
Query: 379 GLHPLGLSTQRTS*HPPQAAPSPNSTVLTATVCTPYPCARVSLYPHAPAVVPDPSRPP*K 200
G H L S PQ P N VL A V P P YPHAP +VPD S PP*K
Sbjct: 351 GRHHLRSPAHLISKQEPQLKPLLNKAVLKAAVFVP*P*LYRFPYPHAPPIVPDASLPP*K 172
Query: 199 VAWA 188
VA A
Sbjct: 171 VACA 160
>gb|CO514640.1|CO514640 s13dSG46C0600050_327940 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 536
Score = 120 bits (256), Expect(2) = 1e-055
Identities = 43/65 (66%), Positives = 53/65 (81%)
Frame = +3 / +3
Query: 450 GGWCNPPREHFDLSMPAFLQIAQEKAGIVPISYRRVACTKQGGIRYTITGNKYFNMVTIT 629
GGWC+PP HFDLS P F IAQ +AGIVP++YRRV C ++GGIR+TI G+ YFN+V +T
Sbjct: 342 GGWCDPPNHHFDLSQPIFQHIAQYRAGIVPVAYRRVRCKRRGGIRFTINGHSYFNLVLVT 521
Query: 630 NVGGA 644
NVGGA
Sbjct: 522 NVGGA 536
Score = 111 bits (238), Expect(2) = 1e-055
Identities = 42/88 (47%), Positives = 55/88 (62%)
Frame = +3 / +3
Query: 141 VVHHNHGKFTAGPXKPAHATFYGGRDGSGTTAGACGYKDTRAQGYGVQTVAVSTVLFGDG 320
+V H +G+ AHATFYGG D SGT GACGY + +QGYG T A+ST LF +G
Sbjct: 63 MVSHVNGRGGHASWINAHATFYGGSDASGTMGGACGYGNLYSQGYGTNTAALSTALFNNG 242
Query: 321 AACGGCYEVRCVDSPSGCKPNAATLVVT 404
+CG CYE++CV+ P C P + + T
Sbjct: 243 LSCGACYEIKCVNDPQWCNPGSIIVTAT 326
Score = 63.4 bits (132), Expect(2) = 1e-022
Identities = 33/70 (47%), Positives = 37/70 (52%)
Frame = -3 / -2
Query: 403 VTTSVAALGLHPLGLSTQRTS*HPPQAAPSPNSTVLTATVCTPYPCARVSLYPHAPAVVP 224
V ++ GLH G T S*H PQ P NS VL A V P P YP AP +VP
Sbjct: 325 VAVTIIDPGLHHCGSFTHLIS*HAPQLNPLLNSAVLNAAVFVP*PWL*RFPYPQAPPIVP 146
Query: 223 DPSRPP*KVA 194
+ S PP*KVA
Sbjct: 145 EASLPP*KVA 116
Score = 57.9 bits (120), Expect(2) = 1e-022
Identities = 31/65 (47%), Positives = 36/65 (55%)
Frame = -3 / -2
Query: 643 APPTLVIVTMLKYLFPVMVYRMPPCFVHATLR*EMGTMPAFSCAIWRNAGMLRSKCSRGG 464
APPT V T LK P+MV +PP +H TL * GT+PA A+ G RSK GG
Sbjct: 535 APPTFVTRTKLK*ECPLMVNLIPPLLLHLTLL*ATGTIPALY*AMC*KIG*ERSKW*LGG 356
Query: 463 LHHPP 449
H PP
Sbjct: 355 SHQPP 341
>gb|CO514666.1|CO514666 s13dSG46E1100087_327992 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 542
Score = 200 bits (432), Expect = 2e-052
Identities = 73/128 (57%), Positives = 93/128 (72%)
Frame = +3 / +1
Query: 159 GKFTAGPXKPAHATFYGGRDGSGTTAGACGYKDTRAQGYGVQTVAVSTVLFGDGAACGGC 338
G +T GP AHATFYGG D SGT GACGY + +QGYGV T A+ST LF +G +CG C
Sbjct: 157 GVYTGGPWTSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNNGLSCGAC 336
Query: 339 YEVRCVDSPSGCKPNAATLVVTATDLCPPNDQQSADSGGWCNPPREHFDLSMPAFLQIAQ 518
+E++C P C P ++++TAT+ CPPN Q +D+GGWCNPPR H DL+MP FL+IAQ
Sbjct: 337 FELKCDQDPRWCNPGNPSILITATNFCPPNFAQPSDNGGWCNPPRPHSDLAMPMFLKIAQ 516
Query: 519 EKAGIVPI 542
+AGIVP+
Sbjct: 517 YRAGIVPV 540
Score = 108 bits (231), Expect = 1e-024
Identities = 60/127 (47%), Positives = 69/127 (54%)
Frame = -3 / -1
Query: 538 GTMPAFSCAIWRNAGMLRSKCSRGGLHHPPLSADCWSLGGHRSVAVTTSVAALGLHPLGL 359
GT+PA AI RN G+ RS+ GGLH PPLS GG + VAV GLH G
Sbjct: 536 GTIPARY*AILRNIGIARSE*GLGGLHQPPLSLG*AKFGGQKFVAVIKIDGFPGLHQRGS 357
Query: 358 STQRTS*HPPQAAPSPNSTVLTATVCTPYPCARVSLYPHAPAVVPDPSRPP*KVAWAGFX 179
+ +S H PQ P NS VL+A V TP P YP AP +VP+ S PP*KVA A
Sbjct: 356 *SHLSSKHAPQLKPLLNSAVLSAAVFTP*PWLYRFPYPQAPPIVPEASLPP*KVA*ALVQ 177
Query: 178 GPAVNLP 158
GP V P
Sbjct: 176 GPPV*TP 156
>gb|CO515743.1|CO515743 s13dSG77C1100086_419635 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 538
Score = 111 bits (238), Expect(2) = 2e-045
Identities = 42/77 (54%), Positives = 50/77 (64%)
Frame = +3 / +1
Query: 174 GPXKPAHATFYGGRDGSGTTAGACGYKDTRAQGYGVQTVAVSTVLFGDGAACGGCYEVRC 353
G + AHATFYGG D SGT GACGY + +QGYG T A+ST LF G +CG CYE+RC
Sbjct: 178 GGWESAHATFYGGGDASGTMGGACGYGNLYSQGYGTNTAALSTALFNSGLSCGSCYEMRC 357
Query: 354 VDSPSGCKPNAATLVVT 404
D P CKP + + T
Sbjct: 358 NDDPRWCKPGSIIVTAT 408
Score = 85.8 bits (181), Expect(2) = 2e-045
Identities = 29/50 (58%), Positives = 41/50 (82%)
Frame = +3 / +1
Query: 390 TLVVTATDLCPPNDQQSADSGGWCNPPREHFDLSMPAFLQIAQEKAGIVP 539
+++VTAT+ CPPN ++GGWCNPP +HFD++ PA+LQIA+ +AGIVP
Sbjct: 388 SIIVTATNFCPPNPSLPNNNGGWCNPPLQHFDMAEPAYLQIAEYRAGIVP 537
Score = 72.1 bits (151), Expect(2) = 1e-023
Identities = 34/70 (48%), Positives = 39/70 (55%)
Frame = -3 / -3
Query: 403 VTTSVAALGLHPLGLSTQRTS*HPPQAAPSPNSTVLTATVCTPYPCARVSLYPHAPAVVP 224
V ++ GLH LG S S* PQ P N VL+A V PYP YPHAP +VP
Sbjct: 407 VAVTIIEPGLHHLGSSLHLIS*QDPQLKPLLNKAVLSAAVLVPYPWLYKFPYPHAPPIVP 228
Query: 223 DPSRPP*KVA 194
+ S PP*KVA
Sbjct: 227 EASPPP*KVA 198
Score = 52.8 bits (109), Expect(2) = 1e-023
Identities = 25/47 (53%), Positives = 28/47 (59%)
Frame = -3 / -3
Query: 538 GTMPAFSCAIWRNAGMLRSKCSRGGLHHPPLSADCWSLGGHRSVAVT 398
GT+PA AI R AG SKC +GGLH PPL GG + VAVT
Sbjct: 536 GTIPALYSAI*R*AGSAISKCCKGGLHQPPLLLGKDGFGGQKFVAVT 396
>gb|CO515120.1|CO515120 s13dSG40E0200019_399105 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 314
Score = 61.1 bits (127), Expect(2) = 2e-021
Identities = 23/54 (42%), Positives = 30/54 (55%)
Frame = +3 / +3
Query: 681 RVKWTEMKRNWGQVWQTGEDLTCESLTFRVMTSDHRKATSWHVLPADWKFGVTY 842
R +W M RNWGQ WQ+ L +SL+ V T + S++V P W FG TY
Sbjct: 99 RTRWQAMSRNWGQNWQSNSYLNGQSLSVVVTTGNGHSIVSFNVAPPSWSFGQTY 260
Score = 56.1 bits (116), Expect(2) = 2e-021
Identities = 19/32 (59%), Positives = 28/32 (87%)
Frame = +3 / +3
Query: 585 YTITGNKYFNMVTITNVGGAGDIAAVSVKGSK 680
+TI G+ YFN+V +TNVGGAGD+ +V++KGS+
Sbjct: 6 FTINGHSYFNLVLVTNVGGAGDVHSVAIKGSR 101
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,727,224
Number of Sequences: 7669
Number of extensions: 118820
Number of successful extensions: 1899
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 292178718
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)