TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3106097.2.2
(1005 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF322116.2|AF322116 Medicago sativa sucrose-phosphate sy... 201 4e-067
gb|AY468362.2| Medicago sativa sucrose-phosphate synthase g... 107 3e-055
>gb|AF322116.2|AF322116 Medicago sativa sucrose-phosphate synthase mRNA, complete cds
Length = 3579
Score = 201 bits (433), Expect(2) = 4e-067
Identities = 81/143 (56%), Positives = 105/143 (73%)
Frame = +3 / +2
Query: 195 EKNKERGQKIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSK 374
+K E ++I E+ S+ YC +FK APP+KE+R+ MRIQALRCH +Y +G++
Sbjct: 2816 DKKGESNEQIVSPVEQLSTDYCYAFKVRKPGMAPPLKELRKLMRIQALRCHPIYCQNGTR 2995
Query: 375 LNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTA 554
LNVIPVLASRSQALRYLY+RWG ELS + V VGECGDTDYEGL+GG+HK++ILKG + A
Sbjct: 2996 LNVIPVLASRSQALRYLYVRWGFELSKMVVFVGECGDTDYEGLVGGLHKSVILKGVGSRA 3175
Query: 555 PNQVHANRSYSSQDVVSFDKQGI 623
+Q+H NR+Y DV+ D I
Sbjct: 3176 ISQLHNNRNYPLSDVMPMDSPNI 3244
Score = 91.8 bits (194), Expect(2) = 2e-024
Identities = 47/95 (49%), Positives = 57/95 (60%)
Frame = -3 / -1
Query: 469 TITVMFDSSTPHRIYKYLKACEREAKTGITFNLLPSWLYKTWQRSACILIVLLISFTGGA 290
T T +FDSS PHR Y+YL+A ER+A+TGITF+L+P Y WQR A ILI L SF GGA
Sbjct: 3090 TNTTIFDSSKPHRTYRYLRAWERDARTGITFSLVPF*QYIGWQRRAWILINLRSSFRGGA 2911
Query: 289 AAVLDPLNEMQ*VDEHSSSSINIFCPLSLFFSAAQ 185
L Q* E S+ + I LS F S +
Sbjct: 2910 IPGFRTLKA*Q*SVESCSTGLTICSLLSPFLSVVE 2806
Score = 68.9 bits (144), Expect(2) = 4e-067
Identities = 26/44 (59%), Positives = 33/44 (75%)
Frame = +3 / +2
Query: 87 LNPAELPFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKNKERGQ 218
LN + F+ DL +HS IEYRWGGEGLRKTL+RWAA ++G+
Sbjct: 2699 LNSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGE 2830
Score = 35.0 bits (70), Expect(2) = 2e-024
Identities = 14/36 (38%), Positives = 23/36 (63%)
Frame = -2 / -3
Query: 194 SCPTN*CLP*TFSSPAIFNLGVIIKINHEWELSRVK 87
SCP * L FS+P +FN+ V I++ H+ + R++
Sbjct: 2806 SCPAY*SLSQPFSTPTVFNVRVEIQVTHK*AIFRIE 2699
>gb|AY468362.2| Medicago sativa sucrose-phosphate synthase gene, promoter region and
complete cds
Length = 8933
Score = 107 bits (229), Expect(3) = 3e-055
Identities = 41/71 (57%), Positives = 53/71 (74%)
Frame = +3 / +2
Query: 411 ALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTAPNQVHANRSYSS 590
+ RYLY+RWG ELS + V VGECGDTDYEGL+GG+HK++ILKG + A +Q+H NR+Y
Sbjct: 8423 SFRYLYVRWGFELSKMVVFVGECGDTDYEGLVGGLHKSVILKGVGSRAISQLHNNRNYPL 8602
Query: 591 QDVVSFDKQGI 623
DV+ D I
Sbjct: 8603 SDVMPMDSPNI 8635
Score = 84.0 bits (177), Expect(3) = 3e-055
Identities = 33/44 (75%), Positives = 40/44 (90%)
Frame = +3 / +1
Query: 291 APPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLASRSQALRY 422
APP+KE+R+ MRIQALRCH +Y +G++LNVIPVLASRSQALRY
Sbjct: 8023 APPLKELRKLMRIQALRCHPIYCQNGTRLNVIPVLASRSQALRY 8154
Score = 68.9 bits (144), Expect(3) = 3e-055
Identities = 26/44 (59%), Positives = 33/44 (75%)
Frame = +3 / +3
Query: 87 LNPAELPFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKNKERGQ 218
LN + F+ DL +HS IEYRWGGEGLRKTL+RWAA ++G+
Sbjct: 7662 LNSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGE 7793
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,495,403
Number of Sequences: 7669
Number of extensions: 119174
Number of successful extensions: 828
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 267371091
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)