TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3089980.2.1
         (540 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AF201458.1|AF201458  Medicago sativa isoflavone reductase...   122   3e-029
emb|X58078.1|MSISREDMR  M.sativa mRNA for isoflavone reductase     91   4e-024

>gb|AF201458.1|AF201458 Medicago sativa isoflavone reductase-like NAD(P)H-dependent
           oxidoreductase (IRL1) mRNA, complete cds
          Length = 1220

 Score =  122 bits (262), Expect = 3e-029
 Identities = 52/131 (39%), Positives = 77/131 (58%)
 Frame = -2 / +2

                                                                       
Query: 539 RATVFGDDATRAIFVHEADMAAVTVRAVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKT 360
           +  ++GD   +A+F  E D+   T+RAVD P   +K LY++PP  + S   LV LWE K+
Sbjct: 551 KVVIYGDGNPKAVFNKEDDIGTFTIRAVDAPGPWNKILYIKPPKESYSSNELVALWEKKS 730

                                                                       
Query: 359 GRALDKYYMPDEELVNRIRDSPLPLNFQLAMVHATVVAGVCDQTVDAEAGGVEATELYPD 180
           G+   K  +P++ L+  I+++P+P+   L + H   V G     V   + GVEA ELYPD
Sbjct: 731 GKPAQKPSLPEDNLLKDIQEAPIPITVVLPIHHGVFVKGDHTNFVIEPSFGVEAYELYPD 910

                      
Query: 179 VNYVTVHDYLD 147
           V Y TV +YLD
Sbjct: 911 VKYTTVEEYLD 943



>emb|X58078.1|MSISREDMR M.sativa mRNA for isoflavone reductase
          Length = 1181

 Score = 91.3 bits (193), Expect(2) = 4e-024
 Identities = 34/106 (32%), Positives = 62/106 (58%)
 Frame = -2 / +1

                                                                       
Query: 539 RATVFGDDATRAIFVHEADMAAVTVRAVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKT 360
           +  + GD   +  +V EAD+   T+RA +DP  L+K +++R P N  +   ++ LWE K 
Sbjct: 607 KVVILGDGNVKGAYVTEADVGTFTIRAANDPNTLNKAVHIRLPKNYLTQNEVIALWEKKI 786

                                                         
Query: 359 GRALDKYYMPDEELVNRIRDSPLPLNFQLAMVHATVVAGVCDQTVD 222
           G+ L+K Y+ +E+++  I++S  P N+ LA+ H+  + G     +D
Sbjct: 787 GKTLEKTYVSEEQVLKDIQESSFPHNYLLALYHSQQIKGDAVYEID 924




 Score = 33.6 bits (67), Expect(2) = 4e-024
 Identities = 12/23 (52%), Positives = 16/23 (69%)
 Frame = -2 / +1

                                  
Query: 215 AGGVEATELYPDVNYVTVHDYLD 147
           A  +EA+E YPDV Y T  +YL+
Sbjct: 928 AKDIEASEAYPDVTYTTADEYLN 996



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 2,333,809
Number of Sequences: 7669
Number of extensions: 20898
Number of successful extensions: 768
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 128911182
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)