TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3071507.2.1
(921 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF083333.1|AF083333 Medicago sativa cinnamyl-alcohol deh... 278 8e-076
gb|L46856.1|ALFCAD1A Medicago sativa cinnamyl-alcohol dehyd... 278 8e-076
gb|AF083332.1|AF083332 Medicago sativa cinnamyl-alcohol deh... 219 5e-058
emb|Z19573.1|MSCIALDHA M.sativa encoding cinnamyl alcohol d... 219 5e-058
gb|L46857.1|ALFCAD1B Medicago sativa cinnamyl-alcohol dehyd... 219 5e-058
>gb|AF083333.1|AF083333 Medicago sativa cinnamyl-alcohol dehydrogenase (MsaCad1) mRNA,
complete cds
Length = 1390
Score = 278 bits (601), Expect = 8e-076
Identities = 119/201 (59%), Positives = 148/201 (73%)
Frame = -1 / +3
Query: 819 PTGLALDRTAPLLCAGVTVYSPMMRHGLNEPGKXXXXXXXXXXXXVAVKFGKAFGMKVTV 640
P L LD APLLCAG+TVYSPM +G+ EPGK VA+KFGKAFG+KVTV
Sbjct: 555 PDNLPLDAGAPLLCAGITVYSPMKYYGMTEPGKHLGVAGLGGLGHVAIKFGKAFGLKVTV 734
Query: 639 ISTSASKRQEAIENLGADEFLISRDEDQMKAATGTMDGIIDTVSAWHPITPLLALLKPLG 460
ISTS +K EAI+ LGAD FL+S+D ++MKAA GTMD IIDT+SA H + PLL LLK G
Sbjct: 735 ISTSPNKETEAIDKLGADSFLVSKDPEKMKAAMGTMDYIIDTISAAHSLMPLLGLLKLNG 914
Query: 459 QMVIVGAPSKPLELPAYAIVPGGKGVAGNNVGSVRDCQAMLEFAGKHGIGAEVEVIKMDY 280
++V VG PSKPLEL + +V G K + G+N+G +++ Q ML+F GKH I A++E+IKM
Sbjct: 915 KLVTVGLPSKPLELSVFPLVAGRKLIGGSNIGGMKETQEMLDFCGKHNITADIELIKMHE 1094
Query: 279 VNTAMERLEKNDVRYRFVIDV 217
+NTAMERL K DV+YRFVIDV
Sbjct: 1095 INTAMERLHKADVKYRFVIDV 1157
Score = 128 bits (274), Expect(2) = 5e-034
Identities = 66/127 (51%), Positives = 75/127 (59%)
Frame = +2 / -1
Query: 443 PTITICPSGFSSASSGVIGCHADTVSMMPSMVPVAAFIWSSSRLMRNSSAPRFSMASWRL 622
PT+T P FS SSG+ AD VSMM SMVP+AAFI+S S R SAP SMAS L
Sbjct: 931 PTVTSFPFNFSRPSSGIRE*AADIVSMM*SMVPIAAFIFSGSLETRKESAPSLSMASVSL 752
Query: 623 LADVLMTVTFIPKALPNLTAXXXXXXXXXXXXCLPGSLRPWRIMGLYTVTPAQSSGAVRS 802
+VL+TVTF P ALPNL A CLPGS+ P +GLYTV PA SGA S
Sbjct: 751 FGEVLITVTFSPNALPNLIATWPNPPSPATPKCLPGSVIP*YFIGLYTVIPAHRSGAPAS 572
Query: 803 SAKPVGD 823
K G+
Sbjct: 571 RGKLSGN 551
Score = 30.9 bits (61), Expect(2) = 5e-034
Identities = 15/41 (36%), Positives = 26/41 (63%)
Frame = +1 / -3
Query: 319 VLPRELEHGLAVPDAADIVPSHALPARHDGVGRQLERLARR 441
VL E+EH L++ +++ PS LP+ + R+L+ LAR+
Sbjct: 1055 VLAAEIEHLLSLFHPSNVTPSDKLPSCN*WKDRELKGLARQ 933
>gb|L46856.1|ALFCAD1A Medicago sativa cinnamyl-alcohol dehydrogenase (cad1) mRNA, complete
cds
Length = 1390
Score = 278 bits (601), Expect = 8e-076
Identities = 119/201 (59%), Positives = 148/201 (73%)
Frame = -1 / +3
Query: 819 PTGLALDRTAPLLCAGVTVYSPMMRHGLNEPGKXXXXXXXXXXXXVAVKFGKAFGMKVTV 640
P L LD APLLCAG+TVYSPM +G+ EPGK VA+KFGKAFG+KVTV
Sbjct: 555 PDNLPLDAGAPLLCAGITVYSPMKYYGMTEPGKHLGVAGLGGLGHVAIKFGKAFGLKVTV 734
Query: 639 ISTSASKRQEAIENLGADEFLISRDEDQMKAATGTMDGIIDTVSAWHPITPLLALLKPLG 460
ISTS +K EAI+ LGAD FL+S+D ++MKAA GTMD IIDT+SA H + PLL LLK G
Sbjct: 735 ISTSPNKETEAIDKLGADSFLVSKDPEKMKAAMGTMDYIIDTISAAHSLMPLLGLLKLNG 914
Query: 459 QMVIVGAPSKPLELPAYAIVPGGKGVAGNNVGSVRDCQAMLEFAGKHGIGAEVEVIKMDY 280
++V VG PSKPLEL + +V G K + G+N+G +++ Q ML+F GKH I A++E+IKM
Sbjct: 915 KLVTVGLPSKPLELSVFPLVAGRKLIGGSNIGGMKETQEMLDFCGKHNITADIELIKMHE 1094
Query: 279 VNTAMERLEKNDVRYRFVIDV 217
+NTAMERL K DV+YRFVIDV
Sbjct: 1095 INTAMERLHKADVKYRFVIDV 1157
Score = 128 bits (274), Expect(2) = 5e-034
Identities = 66/127 (51%), Positives = 75/127 (59%)
Frame = +2 / -1
Query: 443 PTITICPSGFSSASSGVIGCHADTVSMMPSMVPVAAFIWSSSRLMRNSSAPRFSMASWRL 622
PT+T P FS SSG+ AD VSMM SMVP+AAFI+S S R SAP SMAS L
Sbjct: 931 PTVTSFPFNFSRPSSGIRE*AADIVSMM*SMVPIAAFIFSGSLETRKESAPSLSMASVSL 752
Query: 623 LADVLMTVTFIPKALPNLTAXXXXXXXXXXXXCLPGSLRPWRIMGLYTVTPAQSSGAVRS 802
+VL+TVTF P ALPNL A CLPGS+ P +GLYTV PA SGA S
Sbjct: 751 FGEVLITVTFSPNALPNLIATWPNPPSPATPKCLPGSVIP*YFIGLYTVIPAHRSGAPAS 572
Query: 803 SAKPVGD 823
K G+
Sbjct: 571 RGKLSGN 551
Score = 30.9 bits (61), Expect(2) = 5e-034
Identities = 15/41 (36%), Positives = 26/41 (63%)
Frame = +1 / -3
Query: 319 VLPRELEHGLAVPDAADIVPSHALPARHDGVGRQLERLARR 441
VL E+EH L++ +++ PS LP+ + R+L+ LAR+
Sbjct: 1055 VLAAEIEHLLSLFHPSNVTPSDKLPSCN*WKDRELKGLARQ 933
>gb|AF083332.1|AF083332 Medicago sativa cinnamyl-alcohol dehydrogenase (MsaCad2) mRNA,
complete cds
Length = 1324
Score = 219 bits (472), Expect = 5e-058
Identities = 95/201 (47%), Positives = 133/201 (66%)
Frame = -1 / +3
Query: 819 PTGLALDRTAPLLCAGVTVYSPMMRHGLNEPGKXXXXXXXXXXXXVAVKFGKAFGMKVTV 640
P GLA ++ APLLCAGVTVYSP+ GL PG + VK KA G VTV
Sbjct: 507 PEGLAPEQVAPLLCAGVTVYSPLSHFGLKTPGLRGGILGLGGVGHMGVKVAKALGHHVTV 686
Query: 639 ISTSASKRQEAIENLGADEFLISRDEDQMKAATGTMDGIIDTVSAWHPITPLLALLKPLG 460
IS+S K++EA+E+LGAD +L+S D M+ A ++D IIDTV HP+ P L+LLK G
Sbjct: 687 ISSSDKKKKEALEDLGADNYLVSSDTVGMQEAADSLDYIIDTVPVGHPLEPYLSLLKIDG 866
Query: 459 QMVIVGAPSKPLELPAYAIVPGGKGVAGNNVGSVRDCQAMLEFAGKHGIGAEVEVIKMDY 280
+++++G + PL+ ++ G K + G+ VGSV++ + MLEF + G+ + +E++ MDY
Sbjct: 867 KLILMGVINTPLQFVTPMVMLGRKSITGSFVGSVKETEEMLEFWKEKGLTSMIEIVTMDY 1046
Query: 279 VNTAMERLEKNDVRYRFVIDV 217
+N A ERLEKNDVRYRFV+DV
Sbjct: 1047 INKAFERLEKNDVRYRFVVDV 1109
>emb|Z19573.1|MSCIALDHA M.sativa encoding cinnamyl alcohol dehydrogenase
Length = 1321
Score = 219 bits (472), Expect = 5e-058
Identities = 95/201 (47%), Positives = 133/201 (66%)
Frame = -1 / +3
Query: 819 PTGLALDRTAPLLCAGVTVYSPMMRHGLNEPGKXXXXXXXXXXXXVAVKFGKAFGMKVTV 640
P GLA ++ APLLCAGVTVYSP+ GL PG + VK KA G VTV
Sbjct: 531 PEGLAPEQVAPLLCAGVTVYSPLSHFGLKTPGLRGGILGLGGVGHMGVKVAKALGHHVTV 710
Query: 639 ISTSASKRQEAIENLGADEFLISRDEDQMKAATGTMDGIIDTVSAWHPITPLLALLKPLG 460
IS+S K++EA+E+LGAD +L+S D M+ A ++D IIDTV HP+ P L+LLK G
Sbjct: 711 ISSSDKKKKEALEDLGADNYLVSSDTVGMQEAADSLDYIIDTVPVGHPLEPYLSLLKIDG 890
Query: 459 QMVIVGAPSKPLELPAYAIVPGGKGVAGNNVGSVRDCQAMLEFAGKHGIGAEVEVIKMDY 280
+++++G + PL+ ++ G K + G+ VGSV++ + MLEF + G+ + +E++ MDY
Sbjct: 891 KLILMGVINTPLQFVTPMVMLGRKSITGSFVGSVKETEEMLEFWKEKGLTSMIEIVTMDY 1070
Query: 279 VNTAMERLEKNDVRYRFVIDV 217
+N A ERLEKNDVRYRFV+DV
Sbjct: 1071 INKAFERLEKNDVRYRFVVDV 1133
>gb|L46857.1|ALFCAD1B Medicago sativa cinnamyl-alcohol dehydrogenase (cad1) mRNA, partial
cds
Length = 1201
Score = 219 bits (472), Expect = 5e-058
Identities = 95/201 (47%), Positives = 133/201 (66%)
Frame = -1 / +3
Query: 819 PTGLALDRTAPLLCAGVTVYSPMMRHGLNEPGKXXXXXXXXXXXXVAVKFGKAFGMKVTV 640
P GLA ++ APLLCAGVTVYSP+ GL PG + VK KA G VTV
Sbjct: 384 PEGLAPEQVAPLLCAGVTVYSPLSHFGLKTPGLRGGILGLGGVGHMGVKVAKALGHHVTV 563
Query: 639 ISTSASKRQEAIENLGADEFLISRDEDQMKAATGTMDGIIDTVSAWHPITPLLALLKPLG 460
IS+S K++EA+E+LGAD +L+S D M+ A ++D IIDTV HP+ P L+LLK G
Sbjct: 564 ISSSDKKKKEALEDLGADNYLVSSDTVGMQEAADSLDYIIDTVPVGHPLEPYLSLLKIDG 743
Query: 459 QMVIVGAPSKPLELPAYAIVPGGKGVAGNNVGSVRDCQAMLEFAGKHGIGAEVEVIKMDY 280
+++++G + PL+ ++ G K + G+ VGSV++ + MLEF + G+ + +E++ MDY
Sbjct: 744 KLILMGVINTPLQFVTPMVMLGRKSITGSFVGSVKETEEMLEFWKEKGLTSMIEIVTMDY 923
Query: 279 VNTAMERLEKNDVRYRFVIDV 217
+N A ERLEKNDVRYRFV+DV
Sbjct: 924 INKAFERLEKNDVRYRFVVDV 986
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,746,466
Number of Sequences: 7669
Number of extensions: 43620
Number of successful extensions: 490
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 241644663
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)