TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3071103.2.1
(570 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA... 175 3e-073
gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA... 175 3e-073
gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, comple... 175 3e-073
>gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA, complete cds
Length = 1267
Score = 175 bits (377), Expect(2) = 3e-073
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = -2 / +2
Query: 560 PSDYCEPRPQWPCAPGKKYFGRGPIQISFNYNYGPAGRAIGVDLLNNPDLVATDPVISFK 381
PS YC+P ++PCA GK+Y+GRGPIQIS+NYNYG GRAIGVDLLNNPDLVATDPVISFK
Sbjct: 518 PSTYCQPSSEFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFK 697
Query: 380 TALWFWMNARDNKPSCHAVITGQW 309
TALWFWM + KPSCH VITG+W
Sbjct: 698 TALWFWMTPQSPKPSCHDVITGRW 769
Score = 131 bits (280), Expect = 9e-032
Identities = 61/105 (58%), Positives = 71/105 (67%)
Frame = +1 / -2
Query: 235 PLMMLVITPYPGAPRPAALSAAVGVHCPVITAWHDGLLSRAFIQNHSAVLKDITGSVATR 414
P ++++T PAA SA G+H PVIT+W DGL IQNH AVLKDITGSVATR
Sbjct: 843 PPFIMLVTVPYPDSLPAARSALEGLHLPVITSWQDGLGDWGVIQNHRAVLKDITGSVATR 664
Query: 415 SGLLRRSTPMARPAGP*L*LKEIWMGPRPKYFLPGAHGHCGRGSQ 549
SGLL +STP+A P P*L* +EIWMGP P Y P AHG+ G Q
Sbjct: 663 SGLLSKSTPIALPHCP*L*FQEIWMGPLP*YCFPEAHGNSELGWQ 529
Score = 114 bits (243), Expect(2) = 3e-073
Identities = 41/59 (69%), Positives = 48/59 (81%)
Frame = -2 / +2
Query: 266 GYGVITNIINGGIECGHGTDPRVTDRIGFYKRYCDVFRIGYGSNLDCDGQRPFNSGLAV 90
GYG +TNIINGG+ECG G D RV DRIGFYKRYCD+ +GYG+NLDC QRPF S L++
Sbjct: 809 GYGTVTNIINGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDCFSQRPFGSSLSL 985
>gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA, complete cds
Length = 1259
Score = 175 bits (377), Expect(2) = 3e-073
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = -2 / +1
Query: 560 PSDYCEPRPQWPCAPGKKYFGRGPIQISFNYNYGPAGRAIGVDLLNNPDLVATDPVISFK 381
PS YC+P ++PCA GK+Y+GRGPIQIS+NYNYG GRAIGVDLLNNPDLVATDPVISFK
Sbjct: 517 PSTYCQPSSEFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFK 696
Query: 380 TALWFWMNARDNKPSCHAVITGQW 309
TALWFWM + KPSCH VITG+W
Sbjct: 697 TALWFWMTPQSPKPSCHDVITGRW 768
Score = 131 bits (280), Expect = 9e-032
Identities = 61/105 (58%), Positives = 71/105 (67%)
Frame = +1 / -1
Query: 235 PLMMLVITPYPGAPRPAALSAAVGVHCPVITAWHDGLLSRAFIQNHSAVLKDITGSVATR 414
P ++++T PAA SA G+H PVIT+W DGL IQNH AVLKDITGSVATR
Sbjct: 842 PPFIMLVTVPYPDSLPAARSALEGLHLPVITSWQDGLGDWGVIQNHRAVLKDITGSVATR 663
Query: 415 SGLLRRSTPMARPAGP*L*LKEIWMGPRPKYFLPGAHGHCGRGSQ 549
SGLL +STP+A P P*L* +EIWMGP P Y P AHG+ G Q
Sbjct: 662 SGLLSKSTPIALPHCP*L*FQEIWMGPLP*YCFPEAHGNSELGWQ 528
Score = 114 bits (243), Expect(2) = 3e-073
Identities = 41/59 (69%), Positives = 48/59 (81%)
Frame = -2 / +1
Query: 266 GYGVITNIINGGIECGHGTDPRVTDRIGFYKRYCDVFRIGYGSNLDCDGQRPFNSGLAV 90
GYG +TNIINGG+ECG G D RV DRIGFYKRYCD+ +GYG+NLDC QRPF S L++
Sbjct: 808 GYGTVTNIINGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDCFSQRPFGSSLSL 984
>gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, complete cds
Length = 987
Score = 175 bits (377), Expect(2) = 3e-073
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = -2 / +1
Query: 560 PSDYCEPRPQWPCAPGKKYFGRGPIQISFNYNYGPAGRAIGVDLLNNPDLVATDPVISFK 381
PS YC+P ++PCA GK+Y+GRGPIQIS+NYNYG GRAIGVDLLNNPDLVATDPVISFK
Sbjct: 487 PSTYCQPSSEFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFK 666
Query: 380 TALWFWMNARDNKPSCHAVITGQW 309
TALWFWM + KPSCH VITG+W
Sbjct: 667 TALWFWMTPQSPKPSCHDVITGRW 738
Score = 131 bits (280), Expect = 9e-032
Identities = 61/105 (58%), Positives = 71/105 (67%)
Frame = +1 / -2
Query: 235 PLMMLVITPYPGAPRPAALSAAVGVHCPVITAWHDGLLSRAFIQNHSAVLKDITGSVATR 414
P ++++T PAA SA G+H PVIT+W DGL IQNH AVLKDITGSVATR
Sbjct: 812 PPFIMLVTVPYPDSLPAARSALEGLHLPVITSWQDGLGDWGVIQNHRAVLKDITGSVATR 633
Query: 415 SGLLRRSTPMARPAGP*L*LKEIWMGPRPKYFLPGAHGHCGRGSQ 549
SGLL +STP+A P P*L* +EIWMGP P Y P AHG+ G Q
Sbjct: 632 SGLLSKSTPIALPHCP*L*FQEIWMGPLP*YCFPEAHGNSELGWQ 498
Score = 114 bits (243), Expect(2) = 3e-073
Identities = 41/59 (69%), Positives = 48/59 (81%)
Frame = -2 / +1
Query: 266 GYGVITNIINGGIECGHGTDPRVTDRIGFYKRYCDVFRIGYGSNLDCDGQRPFNSGLAV 90
GYG +TNIINGG+ECG G D RV DRIGFYKRYCD+ +GYG+NLDC QRPF S L++
Sbjct: 778 GYGTVTNIINGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDCFSQRPFGSSLSL 954
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,729,976
Number of Sequences: 7669
Number of extensions: 46224
Number of successful extensions: 1448
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 138252572
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)