TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2969865.2.1
         (675 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AJ410120.1|AJ410120  AJ410120 Medicago sativa library (Ka...   169   4e-044

>gb|AJ410120.1|AJ410120 AJ410120 Medicago sativa library (Kalo P) Medicago sativa cDNA
           clone U40, mRNA sequence
          Length = 757

 Score =  169 bits (364), Expect(2) = 4e-044
 Identities = 71/86 (82%), Positives = 72/86 (83%)
 Frame = +3 / +3

                                                                       
Query: 87  PMAXXXXXXXXXXXFSTGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLEN 266
           PM            F+TGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLEN
Sbjct: 120 PMEEDAVGKNEEEEFNTGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLEN 299

                                     
Query: 267 VREMWTEVPKTGKGKKKALPVNKDRF 344
           VREMWTEVPKTGKGKKKA PVNKD F
Sbjct: 300 VREMWTEVPKTGKGKKKAQPVNKDPF 377




 Score =  119 bits (254), Expect(2) = 5e-029
 Identities = 55/86 (63%), Positives = 60/86 (69%)
 Frame = -3 / -1

                                                                       
Query: 343 NLSLFTGRAFFLPLPVFGTSVHISLTFSRTMLQCRSKARTRPSNFLLFRQLIRTWVLFLT 164
           N SL TG AFF PLPV GTSVHIS TFSRT+LQC SKA TRP +FLL RQLIRT VLF T
Sbjct: 376 NGSLLTG*AFFFPLPVLGTSVHISRTFSRTILQCLSKALTRPRSFLLLRQLIRTCVLFFT 197

                                     
Query: 163 LIMSTERGPXXXXXXXXXXXXXSAMG 86
           LIMST+ GP             S++G
Sbjct: 196 LIMSTDSGPVLNSSSSFFPTASSSIG 119




 Score = 23.1 bits (44), Expect(2) = 4e-044
 Identities = 6/8 (75%), Positives = 6/8 (75%)
 Frame = +2 / +1

                   
Query: 362 PWGFGHHC 385
           PW F HHC
Sbjct: 397 PWRFCHHC 420




 Score = 22.6 bits (43), Expect(2) = 5e-029
 Identities = 8/9 (88%), Positives = 9/9 (100%)
 Frame = -3 / -2

                    
Query: 385 TMMTESPRR 359
           TMMTESPR+
Sbjct: 420 TMMTESPRK 394



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 5,367,019
Number of Sequences: 7669
Number of extensions: 67252
Number of successful extensions: 884
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 168617540
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)