TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2922138.2.1
(1095 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO511760.1|CO511760 s13dSG01G0500040_103420 Glandular tr... 245 4e-073
gb|CO513710.1|CO513710 s13dSG15C0100002_140202 Glandular tr... 159 2e-047
>gb|CO511760.1|CO511760 s13dSG01G0500040_103420 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 554
Score = 245 bits (530), Expect(2) = 4e-073
Identities = 97/152 (63%), Positives = 123/152 (80%)
Frame = +3 / +3
Query: 198 VDSFGGLKAYVAGPEDSKAAVVLVADVFGFEAPIMRNIADKVASSGYFVVVPDFFHGDPY 377
V+ G L AY+ G +SK+AV+LV+DV+G+EAP +R +ADKVA++GY+VVVPDF +G+PY
Sbjct: 99 VEKLGALNAYIVGSPNSKSAVLLVSDVYGYEAPNLRKLADKVAAAGYYVVVPDFLNGEPY 278
Query: 378 APENVEKPFPVWIKSHTPEKGFEETKQVIGALKEKGVSAVGAAGYCWGAKVVVELAKAHE 557
PEN ++P P+WIK H +KGFE+ K +I A+K KGVS+VGAAG+CWGAKVV EL K+
Sbjct: 279 NPENPDRPLPIWIKDHGTDKGFEDAKPIIEAIKSKGVSSVGAAGFCWGAKVVTELXKSRL 458
Query: 558 IQAAVLLHPSFVTVDDIKEVKCAISILGAEID 653
IQAAVLLHPSFV+VDDIK V SILGAEID
Sbjct: 459 IQAAVLLHPSFVSVDDIKGVDIPFSILGAEID 554
Score = 149 bits (319), Expect(2) = 1e-037
Identities = 80/149 (53%), Positives = 84/149 (56%)
Frame = -3 / -2
Query: 643 APRIEIAHLTSLISSTVTKEGCNSTAAWIS*AFANSTTTFAPQQYPAAPTADTPFSFRAP 464
AP IE T +SST T +GCNSTAAWIS F +S TT APQQ PAAPT +TP F A
Sbjct: 544 APSIENGMSTPFMSSTETNDGCNSTAAWISLDFXSSVTTLAPQQNPAAPTDETPLLFMAS 365
Query: 463 ITCFVSSNPFSGVCDFIHTGKGFSTFXXXXXXXXXXXGTTTKYPDEATLSAMFLIIGASN 284
I F SS P S FI GKG S F GTTT P ATLSA FL GAS
Sbjct: 364 IMGFASSKPLSVP*SFIQIGKGLSGFSGL*GSPLRKSGTTT**PAAATLSASFLKFGASY 185
Query: 283 PKTSATRTTAALESSGPAT*ALSPPKLST 197
P TS TR TA LE P *AL P ST
Sbjct: 184 P*TSETRRTADLELGEPTM*ALRAPSFST 98
Score = 44.6 bits (91), Expect(2) = 4e-073
Identities = 15/28 (53%), Positives = 20/28 (71%)
Frame = +3 / +3
Query: 114 ERERGRMASSQCCDNPPALNPACGKGKV 197
ER++ RM+ ++CC NPP LNP G G V
Sbjct: 18 ERKKERMSGAECCANPPTLNPNAGSGHV 101
Score = 22.6 bits (43), Expect(2) = 1e-037
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = -2 / -1
Query: 176 RVQGWRVIAALRARHSSPLPF 114
R+Q W V A +RHS L F
Sbjct: 80 RIQCWWVCATFCSRHSFFLSF 18
>gb|CO513710.1|CO513710 s13dSG15C0100002_140202 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 423
Score = 159 bits (342), Expect(2) = 2e-047
Identities = 59/102 (57%), Positives = 82/102 (80%)
Frame = +3 / +2
Query: 198 VDSFGGLKAYVAGPEDSKAAVVLVADVFGFEAPIMRNIADKVASSGYFVVVPDFFHGDPY 377
V+ G L AY+ G +SK+AV+LV+DV+G+EAP +R +ADKVA++GY+VVVPDF +G+PY
Sbjct: 116 VEKLGALNAYIVGSPNSKSAVLLVSDVYGYEAPNLRKLADKVAAAGYYVVVPDFLNGEPY 295
Query: 378 APENVEKPFPVWIKSHTPEKGFEETKQVIGALKEKGVSAVGA 503
PEN ++P P+WIK H +KGFE+ K +I A+K KGVS+VGA
Sbjct: 296 NPENPDRPLPIWIKDHGTDKGFEDAKPIIEAIKSKGVSSVGA 421
Score = 44.6 bits (91), Expect(2) = 2e-047
Identities = 15/28 (53%), Positives = 20/28 (71%)
Frame = +3 / +2
Query: 114 ERERGRMASSQCCDNPPALNPACGKGKV 197
ER++ RM+ ++CC NPP LNP G G V
Sbjct: 35 ERKKERMSGAECCANPPTLNPNAGSGHV 118
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,290,981
Number of Sequences: 7669
Number of extensions: 104558
Number of successful extensions: 824
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 294935121
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)