TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2922023.2.1
(1122 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO515609.1|CO515609 s13dSG47E0500039_418277 Glandular tr... 117 7e-047
gb|CO515300.1|CO515300 s13dSG48C1200098_417659 Glandular tr... 166 9e-047
gb|CO511782.1|CO511782 s13dSG02A0400033_103464 Glandular tr... 90 1e-035
gb|CO512362.1|CO512362 s13dSG80A0800053_114238 Glandular tr... 103 1e-034
emb|AJ245868.1|MSA245868 Medicago sativa mRNA for cysteine ... 62 3e-033
>gb|CO515609.1|CO515609 s13dSG47E0500039_418277 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 591
Score = 117 bits (251), Expect(4) = 7e-047
Identities = 48/66 (72%), Positives = 54/66 (81%)
Frame = -2 / +1
Query: 629 APVVTIDGYEDVPANDESALKKAVAHQPVSVAIEASGSHFQFYSEGVFSGRCGTELDHGV 450
A VVTID YEDVPA DE AL+KAVA+QP++VA+E G FQ Y GVF+GRCGT LDHGV
Sbjct: 82 AKVVTIDDYEDVPAYDELALQKAVANQPIAVAVEGGGREFQLYEYGVFTGRCGTALDHGV 261
Query: 449 AAVGYG 432
AAVGYG
Sbjct: 262 AAVGYG 279
Score = 57.0 bits (118), Expect(4) = 7e-047
Identities = 18/32 (56%), Positives = 26/32 (81%)
Frame = -2 / +1
Query: 428 TADGTKYWLVKNSWGPEWGEKGYIRMARDVAA 333
T +G YW+V+NSWG WGE+GYIR+ R++A+
Sbjct: 280 TENGKDYWIVRNSWGGSWGEQGYIRLERNLAS 375
Score = 36.8 bits (74), Expect(4) = 7e-047
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = -2 / +1
Query: 338 AAKEGHCGIAMEASYPVKTSPNP 270
+++ G CGIA+E SYP+K NP
Sbjct: 373 SSRAGKCGIAIEPSYPIKNGQNP 441
Score = 27.2 bits (53), Expect(4) = 7e-047
Identities = 8/22 (36%), Positives = 15/22 (68%)
Frame = -2 / +1
Query: 698 HGGVAAEDAYPYRARQASCKKS 633
+GG+ +E+ YPY+A C ++
Sbjct: 7 NGGIDSEEDYPYKAIDGRCDQN 72
>gb|CO515300.1|CO515300 s13dSG48C1200098_417659 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 588
Score = 166 bits (356), Expect(2) = 9e-047
Identities = 64/97 (65%), Positives = 76/97 (78%)
Frame = -2 / +2
Query: 932 VPASVDWRQKGAVTDVKDQGQCGSCWAFSTIAAVEGINAIKTKNLTSLSEQQLVDCDTKA 753
+P SVDWR++GAV VKDQ CGSCWAFS IAAVEGIN I T +L SLSEQ+LVDCDT
Sbjct: 296 LPESVDWRKEGAVVGVKDQASCGSCWAFSAIAAVEGINKIVTGDLISLSEQELVDCDTSY 475
Query: 752 NAGCNGGLMDYAFQYIAKHGGVAAEDAYPYRARQASC 642
N GCNGGLMDYAF++I +GG+ +E+ YPY+A C
Sbjct: 476 NEGCNGGLMDYAFEFIISNGGIDSEEDYPYKAIDGRC 586
Score = 115 bits (246), Expect = 1e-026
Identities = 55/91 (60%), Positives = 64/91 (70%)
Frame = +1 / -1
Query: 658 ALYGYASSAATPPCLAMYWNA*SMRPPLQPALALVSQSTSCCSDSDVRFLVLIALMPSTA 837
AL G +SS + PP L M A*S+ PPL P+L VSQSTS CSDS++R V I +PSTA
Sbjct: 570 AL*G*SSSESMPPLLMMNSKA*SISPPLHPSLYDVSQSTSSCSDSEIRSPVTILFIPSTA 391
Query: 838 AIVENAQQLPHWPWSLTSVTAPFCLQSTEAG 930
AI ENAQQLP WSLT TAP LQST++G
Sbjct: 390 AIAENAQQLPQLAWSLTPTTAPSFLQSTDSG 298
Score = 36.3 bits (73), Expect(2) = 9e-047
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = -2 / +2
Query: 1112 EFNRRDEPYKLRLNRFGDMTADEFRRHYAGSRVAHHRMFR 993
E N + Y+L L RF D+T +E+R + G+++ +R +
Sbjct: 125 EHNGLNSTYRLGLTRFADLTNEEYRSKFLGTKINPNRRMK 244
>gb|CO511782.1|CO511782 s13dSG02A0400033_103464 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 536
Score = 89.5 bits (189), Expect(3) = 1e-035
Identities = 35/50 (70%), Positives = 41/50 (82%)
Frame = -2 / +2
Query: 581 ESALKKAVAHQPVSVAIEASGSHFQFYSEGVFSGRCGTELDHGVAAVGYG 432
E AL+KAVA+QP++VA+E G FQ Y GVF+GRCGT LDHGVAAVGYG
Sbjct: 5 ELALQKAVANQPIAVAVEGGGREFQLYEYGVFTGRCGTALDHGVAAVGYG 154
Score = 57.0 bits (118), Expect(3) = 1e-035
Identities = 18/32 (56%), Positives = 26/32 (81%)
Frame = -2 / +2
Query: 428 TADGTKYWLVKNSWGPEWGEKGYIRMARDVAA 333
T +G YW+V+NSWG WGE+GYIR+ R++A+
Sbjct: 155 TENGKDYWIVRNSWGGSWGEQGYIRLERNLAS 250
Score = 36.8 bits (74), Expect(3) = 1e-035
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = -2 / +2
Query: 338 AAKEGHCGIAMEASYPVKTSPNP 270
+++ G CGIA+E SYP+K NP
Sbjct: 248 SSRAGKCGIAIEPSYPIKNGQNP 316
>gb|CO512362.1|CO512362 s13dSG80A0800053_114238 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 576
Score = 103 bits (220), Expect(3) = 1e-034
Identities = 40/58 (68%), Positives = 45/58 (77%)
Frame = -2 / +1
Query: 935 DVPASVDWRQKGAVTDVKDQGQCGSCWAFSTIAAVEGINAIKTKNLTSLSEQQLVDCD 762
++P DWR+KGAVT VKDQG CGSCWAFST A+EG N + T L SLSEQQLVDCD
Sbjct: 274 NLPEDFDWREKGAVTPVKDQGSCGSCWAFSTTGALEGANYLATGKLVSLSEQQLVDCD 447
Score = 47.3 bits (97), Expect(3) = 1e-034
Identities = 17/30 (56%), Positives = 23/30 (76%)
Frame = -2 / +1
Query: 752 NAGCNGGLMDYAFQYIAKHGGVAAEDAYPY 663
++GCNGGLM+ AF+YI + GGV +E Y Y
Sbjct: 481 DSGCNGGLMNNAFEYILQSGGVVSEKDYAY 570
Score = 28.6 bits (56), Expect(3) = 1e-034
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -2 / +1
Query: 1076 LNRFGDMTADEFRRHYAG 1023
+ +F D+TA EFRR + G
Sbjct: 160 ITKFSDLTASEFRRQFLG 213
>emb|AJ245868.1|MSA245868 Medicago sativa mRNA for cysteine protease, partial (cyp15a gene)
Length = 890
Score = 61.6 bits (128), Expect(4) = 3e-033
Identities = 25/36 (69%), Positives = 27/36 (75%)
Frame = -2 / +1
Query: 869 CGSCWAFSTIAAVEGINAIKTKNLTSLSEQQLVDCD 762
CGS WAFST A+EG N + T L SLSEQQLVDCD
Sbjct: 1 CGSGWAFSTTGALEGANYLATGKLVSLSEQQLVDCD 108
Score = 59.7 bits (124), Expect(4) = 3e-033
Identities = 25/61 (40%), Positives = 35/61 (57%)
Frame = -2 / +1
Query: 752 NAGCNGGLMDYAFQYIAKHGGVAAEDAYPYRARQASCKKSPAPVVTIDGYEDVPANDESA 573
++GCNGGLM+ AF+YI + GGV +E Y Y R SCK + +V V + DE
Sbjct: 142 DSGCNGGLMNNAFEYILQSGGVVSEKDYAYTGRDGSCKFDKSKIVASVSNFSVVSLDEDQ 321
Query: 572 L 570
+
Sbjct: 322 I 324
Score = 47.3 bits (97), Expect(4) = 3e-033
Identities = 13/22 (59%), Positives = 18/22 (81%)
Frame = -2 / +1
Query: 410 YWLVKNSWGPEWGEKGYIRMAR 345
YW++KNSWG WGE+GY ++ R
Sbjct: 505 YWIIKNSWGQNWGEEGYYKICR 570
Score = 23.5 bits (45), Expect(4) = 3e-033
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = -2 / +1
Query: 464 LDHGVAAVGYG 432
LDHGV VG+G
Sbjct: 433 LDHGVLLVGFG 465
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,410,669
Number of Sequences: 7669
Number of extensions: 72837
Number of successful extensions: 732
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 303204330
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)