TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2763196.2.1
(2766 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated... 111 2e-044
gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular tr... 155 3e-038
gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular tr... 131 4e-037
emb|AX776636.1| Sequence 5 from Patent WO02102841 120 7e-029
emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulatio... 120 7e-029
gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular tr... 52 8e-029
emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulatio... 114 6e-026
emb|AX776635.1| Sequence 4 from Patent WO02102841 114 6e-026
gb|CO512977.1|CO512977 s13dSG09B0200013_121880 Glandular tr... 74 5e-024
gb|CO515446.1|CO515446 s13dSG50H0600060_417951 Glandular tr... 97 8e-021
>gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated receptor-like kinase
mRNA, complete cds
Length = 1798
Score = 111 bits (236), Expect(3) = 2e-044
Identities = 54/152 (35%), Positives = 85/152 (55%)
Frame = -2 / +1
Query: 896 LDWKKRFDIIEGIARGLLYLHRDSRLRVVHRDLKASNILLDADMKPKISDFGMARMFGGD 717
L+ +R DI IA + YLH + ++HRD+KASNIL+ +++ K++DFG AR+
Sbjct: 844 LEIGERLDIAIDIAHAITYLHMYTDNPIIHRDIKASNILISENLRAKVADFGFARLSEDP 1023
Query: 716 QNQFNTNRVVGTFGYMSPEYAMEGIFSVKSDVYGFGVLILEIITGKRAVSFHCHEDSLNI 537
+ +V GT GYM PEY + KSDVY FGVL++E++TG+ V D
Sbjct: 1024 GATHISTQVKGTAGYMDPEYLRTYQLTEKSDVYSFGVLLVEMMTGRHPVEPKKKIDERVT 1203
Query: 536 AGYAWRQWNEDNAAELIDPVIRASCSVRQVLR 441
+A + +A +DP +R S + +V++
Sbjct: 1204 IRWAMKMLKNGDAVFAMDPRLRRSPASIKVVK 1299
Score = 51.9 bits (107), Expect(3) = 2e-044
Identities = 21/42 (50%), Positives = 28/42 (66%)
Frame = -2 / +1
Query: 1214 YSLDRIRTATSNFSDSNKLGEGGFGPVYMGTLPGGEEVAVKR 1089
++ + I +T+ FS N++GEGGFG VY G L G VAVKR
Sbjct: 526 FTFEEIYKSTAKFSSDNQIGEGGFGTVYRGKLNDGTIVAVKR 651
Score = 51.0 bits (105), Expect(3) = 2e-044
Identities = 21/48 (43%), Positives = 32/48 (66%)
Frame = -2 / +1
Query: 1064 LEEFKNEVILIAKLQHRNLVRLLGCCIPREEKILVYEYMPNKSLDAFL 921
L EFKNE+ ++K++H NLVRL G +EK+++ EY+ N +L L
Sbjct: 679 LYEFKNEIYTLSKIEHLNLVRLYGYLEHGDEKLIIVEYVGNGNLTEHL 822
>gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 611
Score = 155 bits (332), Expect = 3e-038
Identities = 67/162 (41%), Positives = 100/162 (61%)
Frame = -2 / +1
Query: 1055 FKNEVILIAKLQHRNLVRLLGCCIPREEKILVYEYMPNKSLDAFLFNPEKQRLLDWKKRF 876
F NE+ +++L H+NLV+LLG + +ILVYEYM N SL+ L + ++ W R
Sbjct: 124 FVNELESLSRLHHKNLVKLLGFYEDKNGRILVYEYMNNGSLNDHLHKFQTSTIMSWNGRI 303
Query: 875 DIIEGIARGLLYLHRDSRLRVVHRDLKASNILLDADMKPKISDFGMARMFGGDQNQFNTN 696
+ ARG+ YLH+ ++ ++HRD+K SNILLD+ K+SDFG++ M D+ +
Sbjct: 304 KVALDAARGIEYLHKYAQPPIIHRDIKTSNILLDSKWVAKVSDFGLSLMGPEDEESHLSL 483
Query: 695 RVVGTFGYMSPEYAMEGIFSVKSDVYGFGVLILEIITGKRAV 570
GT GYM PEY + KSDVY FGV +LE+++G +A+
Sbjct: 484 LAAGTVGYMDPEYYRLQYLTSKSDVYSFGVXLLELLSGYKAI 609
>gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 595
Score = 131 bits (280), Expect(2) = 4e-037
Identities = 56/127 (44%), Positives = 78/127 (61%)
Frame = -2 / +1
Query: 905 QRLLDWKKRFDIIEGIARGLLYLHRDSRLRVVHRDLKASNILLDADMKPKISDFGMARMF 726
Q+ LDW R I G ARGL YLH + V++RDLK SNILL D K+SDFG+A++
Sbjct: 43 QKPLDWNTRMKIAAGAARGLEYLHDKMKPPVIYRDLKCSNILLGEDYHSKLSDFGLAKVG 222
Query: 725 GGDQNQFNTNRVVGTFGYMSPEYAMEGIFSVKSDVYGFGVLILEIITGKRAVSFHCHEDS 546
+ RV+GT+GY +P+YAM G + KSD+Y GV +LE+ITG++A+
Sbjct: 223 PIGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSIGVALLELITGRKAIDHRRPAKE 402
Query: 545 LNIAGYA 525
N+ +A
Sbjct: 403 QNLVAWA 423
Score = 40.0 bits (81), Expect(2) = 4e-037
Identities = 16/46 (34%), Positives = 28/46 (60%)
Frame = -2 / +1
Query: 497 AELIDPVIRASCSVRQVLRCIHIALLCVQDHADERPDIPTVILMLS 360
+E+IDP++ VR + + + IA +CVQ+ + RP I V+ L+
Sbjct: 454 SEMIDPLLEGQYPVRGLYQALAIAAMCVQEQPNMRPVIADVVTALN 591
>emb|AX776636.1| Sequence 5 from Patent WO02102841
Length = 3339
Score = 120 bits (257), Expect(2) = 7e-029
Identities = 52/123 (42%), Positives = 75/123 (60%)
Frame = -2 / +3
Query: 935 LDAFLFNPEKQRLLDWKKRFDIIEGIARGLLYLHRDSRLRVVHRDLKASNILLDADMKPK 756
LD K+++LDW R I G ARGL YLH V+HRD+K+SNILLD M K
Sbjct: 2277 LDRLYGEASKRKILDWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAK 2456
Query: 755 ISDFGMARMFGGDQNQFNTNRVVGTFGYMSPEYAMEGIFSVKSDVYGFGVLILEIITGKR 576
++DFG ++ + + + + V GT GY+ PEY S KSDV+ FGV++LEI++G+
Sbjct: 2457 VADFGFSKYAPQEGDSYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGRE 2636
Query: 575 AVS 567
++
Sbjct: 2637 PLN 2645
Score = 23.1 bits (44), Expect(2) = 7e-029
Identities = 13/33 (39%), Positives = 15/33 (45%)
Frame = -1 / +2
Query: 1152 RRIWSCLYGNFTWGRRSSCEEAL*EFRSRP*RI 1054
RRIW CL + RS E A+ S RI
Sbjct: 2057 RRIWFCLQRHSRRWSRSGSESAVIHINSGNPRI 2155
>emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulation receptor kinase (nork gene), wild
type allele DMI2
Length = 3339
Score = 120 bits (257), Expect(2) = 7e-029
Identities = 52/123 (42%), Positives = 75/123 (60%)
Frame = -2 / +3
Query: 935 LDAFLFNPEKQRLLDWKKRFDIIEGIARGLLYLHRDSRLRVVHRDLKASNILLDADMKPK 756
LD K+++LDW R I G ARGL YLH V+HRD+K+SNILLD M K
Sbjct: 2277 LDRLYGEASKRKILDWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAK 2456
Query: 755 ISDFGMARMFGGDQNQFNTNRVVGTFGYMSPEYAMEGIFSVKSDVYGFGVLILEIITGKR 576
++DFG ++ + + + + V GT GY+ PEY S KSDV+ FGV++LEI++G+
Sbjct: 2457 VADFGFSKYAPQEGDSYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGRE 2636
Query: 575 AVS 567
++
Sbjct: 2637 PLN 2645
Score = 23.1 bits (44), Expect(2) = 7e-029
Identities = 13/33 (39%), Positives = 15/33 (45%)
Frame = -1 / +2
Query: 1152 RRIWSCLYGNFTWGRRSSCEEAL*EFRSRP*RI 1054
RRIW CL + RS E A+ S RI
Sbjct: 2057 RRIWFCLQRHSRRWSRSGSESAVIHINSGNPRI 2155
>gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 596
Score = 52.4 bits (108), Expect(4) = 8e-029
Identities = 22/51 (43%), Positives = 32/51 (62%)
Frame = -2 / +1
Query: 686 GTFGYMSPEYAMEGIFSVKSDVYGFGVLILEIITGKRAVSFHCHEDSLNIA 534
GT GY+ PEY + KSD Y FGV+++E+I+ RAV H + +N+A
Sbjct: 436 GTPGYVDPEYYQCYQLTDKSDAYSFGVVLVELISSLRAVDITRHRNDVNLA 588
Score = 51.0 bits (105), Expect(4) = 8e-029
Identities = 22/56 (39%), Positives = 35/56 (62%)
Frame = -2 / +1
Query: 1127 GTLPGGEEVAVKRLCRNSGQGLEEFKNEVILIAKLQHRNLVRLLGCCIPREEKILV 960
G L G VAVKR ++ + + +F NEV ++A+L+H+NLV L GC ++L+
Sbjct: 1 GDLKDGRVVAVKRHYESNFKRVAQFMNEVEILARLRHKNLVTLYGCTSKHSRELLL 168
Score = 49.2 bits (101), Expect(4) = 8e-029
Identities = 18/39 (46%), Positives = 28/39 (71%)
Frame = -2 / +1
Query: 815 VVHRDLKASNILLDADMKPKISDFGMARMFGGDQNQFNT 699
V+HRD+K++NILLD K++DFG++R+F D +T
Sbjct: 310 VMHRDVKSNNILLDEKFHVKVADFGLSRLFPNDVTHVST 426
Score = 25.8 bits (50), Expect(4) = 8e-029
Identities = 11/22 (50%), Positives = 11/22 (50%)
Frame = -2 / +1
Query: 899 LLDWKKRFDIIEGIARGLLYLH 834
LL W R DI A L YLH
Sbjct: 235 LLPWSVRLDIALETAEALAYLH 300
>emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulation receptor kinase (nork gene),
mutant allele nn1
Length = 3337
Score = 114 bits (243), Expect = 6e-026
Identities = 51/123 (41%), Positives = 73/123 (59%)
Frame = -2 / +1
Query: 935 LDAFLFNPEKQRLLDWKKRFDIIEGIARGLLYLHRDSRLRVVHRDLKASNILLDADMKPK 756
LD K+++LDW R I G RGL LH V+HRDLK+SNILLD M K
Sbjct: 2275 LDRLYGEASKRKILDWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAK 2454
Query: 755 ISDFGMARMFGGDQNQFNTNRVVGTFGYMSPEYAMEGIFSVKSDVYGFGVLILEIITGKR 576
++DFG ++ + + + + V GT GY+ PEY S KSDV+ FGV++LEI++G+
Sbjct: 2455 VADFGFSKYAPQEGDSYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGRE 2634
Query: 575 AVS 567
++
Sbjct: 2635 PLN 2643
>emb|AX776635.1| Sequence 4 from Patent WO02102841
Length = 3378
Score = 114 bits (243), Expect = 6e-026
Identities = 51/123 (41%), Positives = 73/123 (59%)
Frame = -2 / +1
Query: 935 LDAFLFNPEKQRLLDWKKRFDIIEGIARGLLYLHRDSRLRVVHRDLKASNILLDADMKPK 756
LD K+++LDW R I G RGL LH V+HRDLK+SNILLD M K
Sbjct: 2296 LDRLYGEASKRKILDWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAK 2475
Query: 755 ISDFGMARMFGGDQNQFNTNRVVGTFGYMSPEYAMEGIFSVKSDVYGFGVLILEIITGKR 576
++DFG ++ + + + + V GT GY+ PEY S KSDV+ FGV++LEI++G+
Sbjct: 2476 VADFGFSKYAPQEGDSYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGRE 2655
Query: 575 AVS 567
++
Sbjct: 2656 PLN 2664
>gb|CO512977.1|CO512977 s13dSG09B0200013_121880 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 582
Score = 73.9 bits (155), Expect(3) = 5e-024
Identities = 31/56 (55%), Positives = 38/56 (67%)
Frame = -2 / +1
Query: 896 LDWKKRFDIIEGIARGLLYLHRDSRLRVVHRDLKASNILLDADMKPKISDFGMARM 729
L W R I++G A+GL+YLH S + VH DLK SNILL DM P ISDFG+ R+
Sbjct: 175 LTWSDRLKIMKGTAKGLVYLHEFSPKKYVHGDLKPSNILLGHDMTPYISDFGLGRL 342
Score = 45.1 bits (92), Expect(3) = 5e-024
Identities = 19/42 (45%), Positives = 27/42 (64%)
Frame = -2 / +1
Query: 1058 EFKNEVILIAKLQHRNLVRLLGCCIPREEKILVYEYMPNKSL 933
EF+ EV I KL+H N+ L +EK+L+Y+Y+PN SL
Sbjct: 4 EFQTEVEAIGKLRHPNIATLRAYYWSVDEKLLIYDYIPNGSL 129
Score = 32.7 bits (65), Expect(3) = 5e-024
Identities = 12/20 (60%), Positives = 17/20 (85%)
Frame = -2 / +1
Query: 638 SVKSDVYGFGVLILEIITGK 579
S K DVY +GV++LE+ITG+
Sbjct: 496 SQKWDVYSYGVILLEMITGR 555
>gb|CO515446.1|CO515446 s13dSG50H0600060_417951 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 531
Score = 97.3 bits (206), Expect = 8e-021
Identities = 39/94 (41%), Positives = 61/94 (64%)
Frame = -2 / +3
Query: 863 GIARGLLYLHRDSRLRVVHRDLKASNILLDADMKPKISDFGMARMFGGDQNQFNTNRVVG 684
G ARGL +LH ++ +++ ++ IL+D + +I DFG+AR+ D N N +G
Sbjct: 21 GAARGLAWLHHGCHPPIIQQNICSNVILVDEEFDARIMDFGLARLMTSDANGSFVNGDLG 200
Query: 683 TFGYMSPEYAMEGIFSVKSDVYGFGVLILEIITG 582
GY++PEY+ + S+K DVYGFGVL+LE++TG
Sbjct: 201 ELGYIAPEYSSTMVASLKGDVYGFGVLLLELVTG 302
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 24,464,850
Number of Sequences: 7669
Number of extensions: 380802
Number of successful extensions: 2428
Number of sequences better than 1.0e-020: 13
length of database: 1,248,568
effective HSP length: 46
effective length of database: 895,794
effective search space used: 783819750
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)