TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2623104.2.4
(1628 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA... 280 7e-098
gb|AY219848.1| Medicago sativa polygalactorunase PG11 precu... 219 2e-089
emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene 184 5e-077
>gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA, complete cds
Length = 1492
Score = 280 bits (605), Expect(2) = 7e-098
Identities = 108/218 (49%), Positives = 154/218 (70%)
Frame = +2 / +1
Query: 533 DFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHMGDSSGITITN 712
+FVNN V GVT +SK FH+ ++ CK++ T+TAPGDSPNTDGIHMG S+ + I N
Sbjct: 487 NFVNNSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDGIHMGKSTDVKILN 666
Query: 713 TVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTM 892
T IG GDDC+SIG G+ ++ + GV CGPGHG+S+GSLG++ E++V I VK+CTL T
Sbjct: 667 TNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVEGITVKNCTLTATD 846
Query: 893 FGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGASKVTVKDVT 1072
GVRIK + DA +TVS IH+E+I M + NP+ ID +Y P C+ SK+ + ++
Sbjct: 847 NGVRIKTWPDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCSKKNPSKIKLSKIS 1026
Query: 1073 FKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSG 1186
FKN+ GTS T E V L+C++ VPC GV +++V+++++G
Sbjct: 1027 FKNVKGTSGTAEGVVLICSSAVPCDGVELNNVDLKFNG 1140
Score = 167 bits (360), Expect(2) = 6e-043
Identities = 94/225 (41%), Positives = 115/225 (51%)
Frame = -3 / -3
Query: 1209 IAMVLLLVPLYSTLTSSMVTPVHGTLAVQSRLTASGVEEVPVMFLKVTSLTVTLEAPLAV 1030
+ + ++ PL T TP HGT +Q R T S V EVP+ FL L +
Sbjct: 1163 VHLAVVGAPLNLRSTLFSSTPSHGTALLQIRTTPSAVPEVPLTFLNEILLNFIFDGFFLE 984
Query: 1029 HNLLGQYFMSMKMGLAESSILIFS*WILETVSTEAASSYALMRTPNIVFLRVQSLTLMSV 850
H G Y SM G ++I S I ETV AS L+ TP V +RVQ LT++
Sbjct: 983 HWFHG*YSWSMMTGFLTFVMVISSKCISETVIVPGASGQVLILTPLSVAVRVQFLTVIPS 804
Query: 849 TSFSSLYRPRLPMLMPWPGPQVTPVMFTLEVPGPMEMQSSPTPMTVLVMVIPDESPMWMP 670
T S + PRLP L P PGPQ TP +P P+E QSSP PM VL ++ + PMW+P
Sbjct: 803 TFSSVVNFPRLPTLRP*PGPQFTPWTVICLLPSPIETQSSPVPMLVLRILTSVDFPMWIP 624
Query: 669 SVLGLSPGAVTVTSLISMSLHRYMFMWKNLELSSVTPDTSPLFTK 535
SVLGLSPGAV V + LH WK L VTP T L TK
Sbjct: 623 SVLGLSPGAVIVKPSNVIFLHPKTITWKFLLSLEVTPRTMELLTK 489
Score = 93.6 bits (198), Expect(2) = 7e-098
Identities = 40/119 (33%), Positives = 61/119 (51%)
Frame = +2 / +1
Query: 182 GNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVTIQVDGNLLAT 361
G +D +A+ AW AC T I+IP G + + + GPCK + +QVDG + A
Sbjct: 124 GKPNSDIGQALTSAWNEACASTTAAKIVIPAGTYQLNGIELKGPCKAPIELQVDGTIQAP 303
Query: 362 TDLSQYKDHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCKILPNSLVMDF 538
D S K W + L +D+L ++GKG DGQG V+ K + + K + + M+F
Sbjct: 304 ADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQGATVYKKAAPASAWSGKNSNSKVFMNF 480
Score = 22.1 bits (42), Expect(2) = 6e-043
Identities = 10/21 (47%), Positives = 11/21 (52%)
Frame = -3 / -3
Query: 468 TAGPCPSRFPLPVMTRLSTRR 406
T P PS+ PLP R S R
Sbjct: 410 TVAP*PSKTPLPDKVRWSIYR 348
>gb|AY219848.1| Medicago sativa polygalactorunase PG11 precursor, gene, complete cds
Length = 2213
Score = 219 bits (472), Expect(3) = 2e-089
Identities = 89/166 (53%), Positives = 112/166 (67%)
Frame = +2 / +1
Query: 536 FVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHMGDSSGITITNT 715
FVNN V G+T +SK FH+ + C + TVTAPGDSPNTDGIHMG S+ I I NT
Sbjct: 1225 FVNNSMVHGLTSKDSKNFHVMVLGCNNFTFDGFTVTAPGDSPNTDGIHMGRSTDIKILNT 1404
Query: 716 VIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTMF 895
I GDDCIS+G G+ K+ + V CGPGHGIS+GSLG+Y E +V + VK+CTL T
Sbjct: 1405 NIATGDDCISLGDGSRKITVQNVKCGPGHGISVGSLGKYTTEDNVEGVIVKNCTLTATQN 1584
Query: 896 GVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCT 1033
GVRIK + DA +TVS IH+E+I M + NP+ ID +YCP C+
Sbjct: 1585 GVRIKTWPDAPGTITVSDIHFEDIIMNNVMNPVIIDQEYCPWNQCS 1722
Score = 132 bits (283), Expect(3) = 8e-036
Identities = 74/164 (45%), Positives = 87/164 (53%)
Frame = -3 / -1
Query: 1029 HNLLGQYFMSMKMGLAESSILIFS*WILETVSTEAASSYALMRTPNIVFLRVQSLTLMSV 850
H GQY SM G ++I S I ETV AS L+ TP V +RVQ LT+
Sbjct: 1718 HWFHGQYSWSMMTGFMTLFMIISSKCISETVIVPGASGQVLILTPF*VAVRVQFLTITPS 1539
Query: 849 TSFSSLYRPRLPMLMPWPGPQVTPVMFTLEVPGPMEMQSSPTPMTVLVMVIPDESPMWMP 670
T S +Y PRLP L+P PGP T +P P E+QSSP M VL ++I + PMW+P
Sbjct: 1538 TLSSVVYFPRLPTLIP*PGPHFTFWTVIFLLPSPNEIQSSPVAMLVLRILISVDLPMWIP 1359
Query: 669 SVLGLSPGAVTVTSLISMSLHRYMFMWKNLELSSVTPDTSPLFT 538
SVLGLSPGAVTV LH WK L V P T L T
Sbjct: 1358 SVLGLSPGAVTVKPSNVKLLHPKTITWKFLLSLEVRPWTMELLT 1227
Score = 92.3 bits (195), Expect(3) = 2e-089
Identities = 37/91 (40%), Positives = 52/91 (57%)
Frame = +2 / +2
Query: 206 KAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVTIQVDGNLLATTDLSQYKD 385
KA+ AW AC T I+IP G + + + GPCK + +QVDG + A D SQ K
Sbjct: 770 KAIGNAWKEACLSTTAAKIMIPGGTYRMNGVELKGPCKAPIEVQVDGTIQAPADPSQMKA 949
Query: 386 HGNWIEILRVDNLVITGKGNLDGQGPAVWSK 478
WI+ L +++L ++GKG DGQG V+ K
Sbjct: 950 ADQWIKFLYMEHLTLSGKGVFDGQGATVYKK 1042
Score = 62.5 bits (130), Expect(3) = 2e-089
Identities = 22/47 (46%), Positives = 35/47 (74%)
Frame = +2 / +3
Query: 1046 SKVTVKDVTFKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSG 1186
SK+ + ++FKNI GTS TPE V L+C++ VPC GV +++V + ++G
Sbjct: 1851 SKIKLSKISFKNIQGTSGTPEGVVLICSSGVPCDGVELNNVGLTFNG 1991
Score = 38.6 bits (78), Expect(3) = 8e-036
Identities = 20/37 (54%), Positives = 21/37 (56%)
Frame = -3 / -2
Query: 1185 PLYSTLTSSMVTPVHGTLAVQSRLTASGVEEVPVMFL 1075
PL T TP HGT +Q R T SGV EVP MFL
Sbjct: 1990 PLNVRPTLFSSTPSHGTPLLQIRTTPSGVPEVP*MFL 1880
Score = 22.1 bits (42), Expect(3) = 8e-036
Identities = 14/43 (32%), Positives = 19/43 (44%)
Frame = -3 / -3
Query: 348 LPSTWMVTSPLQGPVKLSCPTRKSPLGMRIVCFPVPPHADAHA 220
+PST LQGP+ + P G+ I+ V HA A
Sbjct: 912 VPST*TSIGALQGPLSSTPFILYVPPGIMILAAVVDRHASFQA 784
>emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene
Length = 2185
Score = 184 bits (396), Expect(3) = 5e-077
Identities = 71/167 (42%), Positives = 107/167 (64%)
Frame = +2 / +1
Query: 533 DFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHMGDSSGITITN 712
+F+ + V +T +SK FH+N+ C + VT+TAP S NTDGIH+G S+ + + N
Sbjct: 1201 NFLKHSIVRDITSKDSKNFHVNVLGCTNFTFDSVTITAPVTSINTDGIHIGRSTDVKVLN 1380
Query: 713 TVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTM 892
T I GDDC+S+G G+ ++ + V CGPGHGIS+GSLG+Y E+ V + VK+CT+ T
Sbjct: 1381 TNIATGDDCVSLGDGSRQITVQNVNCGPGHGISVGSLGKYPKEEAVEHVLVKNCTITNTD 1560
Query: 893 FGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCT 1033
GVRIK + + ++ +H+E+I M + NP+ ID +YCP C+
Sbjct: 1561 NGVRIKTWPSSPGTSPITDMHFEDIIMVNVLNPVIIDQEYCPWNQCS 1701
Score = 95.9 bits (203), Expect(3) = 5e-077
Identities = 36/100 (36%), Positives = 53/100 (53%)
Frame = +2 / +3
Query: 209 AVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVTIQVDGNLLATTDLSQYKDH 388
A AW +ACG T ++IP+G + + ++ GPCK + +QVDG + A + + D
Sbjct: 450 AFTNAWKAACGSTTASKVMIPRGVYKMHAVDVKGPCKAPIEVQVDGTIQAPQNPDELNDA 629
Query: 389 GNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCK 508
WI+ VD ++GKG DG G W +N C K CK
Sbjct: 630 YQWIKFGYVDYFTLSGKGVFDGNGETAWRQNDCGKNSTCK 749
Score = 92.3 bits (195), Expect(4) = 1e-028
Identities = 54/133 (40%), Positives = 65/133 (48%)
Frame = -3 / -3
Query: 1029 HNLLGQYFMSMKMGLAESSILIFS*WILETVSTEAASSYALMRTPNIVFLRVQSLTLMSV 850
H GQY SM G +++I S I L+ TP VF+ VQ LT
Sbjct: 1697 H*FQGQYSWSMITGFRTLTMIISSKCISVIGDVPGELGQVLILTPLSVFVMVQFLTRTCS 1518
Query: 849 TSFSSLYRPRLPMLMPWPGPQVTPVMFTLEVPGPMEMQSSPTPMTVLVMVIPDESPMWMP 670
T+ S Y P+LP L+PWPGPQ T +P P E QSSP M VL + PM +P
Sbjct: 1517 TASSFGYFPKLPTLIPWPGPQFTFWTVICLLPSPNETQSSPVAMFVLRTFTSVDLPMCIP 1338
Query: 669 SVLGLSPGAVTVT 631
SV L GAV VT
Sbjct: 1337 SVFMLVTGAVMVT 1299
Score = 51.9 bits (107), Expect(3) = 5e-077
Identities = 19/47 (40%), Positives = 30/47 (63%)
Frame = +2 / +2
Query: 1046 SKVTVKDVTFKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSG 1186
SK+ + V FKNI GTS T + V L+C+ PC V +++V + ++G
Sbjct: 1808 SKIKISKVIFKNIRGTSKTKDGVVLICSKSFPCDAVELNNVALTFNG 1948
Score = 37.7 bits (76), Expect(4) = 1e-028
Identities = 20/42 (47%), Positives = 23/42 (54%)
Frame = -3 / -2
Query: 1185 PLYSTLTSSMVTPVHGTLAVQSRLTASGVEEVPVMFLKVTSL 1060
PL T T HG L + R T S V EVP+MFLK+T L
Sbjct: 1947 PLNVRATLFSSTASHGKLLLHIRTTPSLVFEVPLMFLKITLL 1822
Score = 32.2 bits (64), Expect(4) = 1e-028
Identities = 15/42 (35%), Positives = 20/42 (47%)
Frame = -3 / -1
Query: 537 KSITSELGRILQSYFLVQEFLLHTAGPCPSRFPLPVMTRLST 412
K + + R+L F Q F L P PS+ PLP + ST
Sbjct: 778 KLFSFTMERLLHVEFFPQSFCLQAVSPLPSKTPLPDKVK*ST 653
Score = 26.7 bits (52), Expect(4) = 1e-028
Identities = 14/39 (35%), Positives = 19/39 (48%)
Frame = -3 / -1
Query: 348 LPSTWMVTSPLQGPVKLSCPTRKSPLGMRIVCFPVPPHA 232
+PST LQGP + +PLG+ + V PHA
Sbjct: 589 VPST*TSIGALQGPFTSTACILYTPLGIITLLAVVDPHA 473
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,398,371
Number of Sequences: 7669
Number of extensions: 174260
Number of successful extensions: 1238
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 453743736
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)