TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2550113.2.1
(803 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514508.1|CO514508 s13dSG43E0900069_327676 Glandular tr... 218 8e-058
gb|AW698456.1|AW698456 g409 glandular-haired subtracted cDN... 133 2e-032
>gb|CO514508.1|CO514508 s13dSG43E0900069_327676 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 532
Score = 218 bits (470), Expect = 8e-058
Identities = 92/100 (92%), Positives = 94/100 (94%)
Frame = -2 / +2
Query: 697 KMVQRLTYRKRHSYATKSNQTRVVKTPGGKLVYQYTKKRASGPKCPVTGKKIQGIPHLRP 518
KMVQRLTYR+RHSYATKSNQ RVVKTPGGKLVYQ TKKRASGPKCPVTGK+IQGIPHLRP
Sbjct: 65 KMVQRLTYRRRHSYATKSNQHRVVKTPGGKLVYQTTKKRASGPKCPVTGKRIQGIPHLRP 244
Query: 517 AEYKRSRLSRNRRTVNRPYGGVLSGTAVRERIIRAFLVEE 398
EYKRSRL RNRRTVNR YGGVLSG AVRERIIRAFLVEE
Sbjct: 245 TEYKRSRLPRNRRTVNRAYGGVLSGGAVRERIIRAFLVEE 364
Score = 171 bits (368), Expect = 1e-043
Identities = 77/110 (70%), Positives = 81/110 (73%)
Frame = +3 / -2
Query: 327 QSYFFEEVLSLVFCNFNTFLTIFCSSTRKARMILSLTAVPDSTPP*GRFTVLRFLDNLDL 506
Q+Y E S F TF TIFCSST+KA MILS TA PD TPP* RFTVLRFL NLDL
Sbjct: 435 QNYALEATFSFAF*ILRTFFTIFCSSTKKALMILSRTAPPDKTPP*ARFTVLRFLGNLDL 256
Query: 507 LYSAGLRWGMPWIFFPVTGHLGPLALFLVYWYTSLPPGVLTTRVWLDFVA 656
LYS GL+ G+P I PVTGHLGPLALFLV TSLPPGVLTT WLD VA
Sbjct: 255 LYSVGLKCGIP*ILLPVTGHLGPLALFLVV**TSLPPGVLTTLCWLDLVA 106
Score = 102 bits (218), Expect = 5e-023
Identities = 48/95 (50%), Positives = 61/95 (64%)
Frame = +2 / -1
Query: 410 ESTDDPFSNCSSR*YSPIGTVHSSAVPRQPGPFVLSWPQMGNALDLLPGHGTLGPAGSLL 589
ES +DPF N +SR S I TVHS V RQP VL PQ+ N+L+ L + L A SLL
Sbjct: 352 ESPNDPFPNSTSRQNSAISTVHSPTVSRQP*SLVLCRPQVWNSLNSLASNRALRSASSLL 173
Query: 590 GVLVHELATRGLDDPRLVGLRGIAVPLAVGQTLHH 694
L+++L TRG D+P LVG +AV A+G+TLHH
Sbjct: 172 SSLINKLTTRGFDNPVLVGFGCVAVTTAIGETLHH 68
Score = 97.7 bits (207), Expect = 2e-021
Identities = 49/138 (35%), Positives = 75/138 (54%)
Frame = -1 / +1
Query: 731 GFIHXXXXXXRQDGAASDLPQAAQLCHEVQPDAGRQDPWWQARVPVHQEESQRAQVSRDR 552
G + ++GA S L ++QL +++QP G Q+PWW A + + EES R V
Sbjct: 31 GEVEAAKSLSIENGATSHLSPSSQLRNQIQPTQGCQNPWW*ACLSDY*EES*RT*VPCYW 210
Query: 551 EEDPGHSPSEASXXXXXXXX*EPQNCEPSLWGSTIWNCS*RKDHPCFPG*RAEDCQEGVE 372
+E+ +S EA *+P +CEP LW S +W C KDH F G*RA+DC++G +
Sbjct: 211 QENSRNSTLEAYRVQEIKVA*KP*DCEPCLWRSFVWRCCSGKDH*GFLG*RAKDCEKGPQ 390
Query: 371 ITEDQRQDFLKKVTLKQK 318
+E + + L+ + L K
Sbjct: 391 NSEGKGKGCLQGIILDHK 444
>gb|AW698456.1|AW698456 g409 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 220
Score = 133 bits (286), Expect = 2e-032
Identities = 55/62 (88%), Positives = 57/62 (91%)
Frame = -2 / -1
Query: 697 KMVQRLTYRKRHSYATKSNQTRVVKTPGGKLVYQYTKKRASGPKCPVTGKKIQGIPHLRP 518
KMVQRLTYR+R YATKSNQ RVVKTPGGKLVYQ TKKRASGPKCPVTGK+IQGIPHLRP
Sbjct: 187 KMVQRLTYRRRLRYATKSNQHRVVKTPGGKLVYQTTKKRASGPKCPVTGKRIQGIPHLRP 8
Query: 517 AE 512
E
Sbjct: 7 TE 2
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,798,356
Number of Sequences: 7669
Number of extensions: 87717
Number of successful extensions: 1212
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 208455750
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)