TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493976.2.1
(1294 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO513205.1|CO513205 s13dSG24B0800061_129574 Glandular tr... 180 2e-072
gb|CO514640.1|CO514640 s13dSG46C0600050_327940 Glandular tr... 138 1e-065
gb|CO515743.1|CO515743 s13dSG77C1100086_419635 Glandular tr... 109 3e-050
gb|CO514666.1|CO514666 s13dSG46E1100087_327992 Glandular tr... 111 5e-050
gb|CO515120.1|CO515120 s13dSG40E0200019_399105 Glandular tr... 100 5e-037
>gb|CO513205.1|CO513205 s13dSG24B0800061_129574 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 592
Score = 180 bits (387), Expect(2) = 2e-072
Identities = 67/88 (76%), Positives = 79/88 (89%)
Frame = +2 / +2
Query: 374 GGGRSCLPGSVVVTATNFCPPNNALPSDDGGWCNPPRAHFDMSQPVFQRIALYRAGIVPV 553
G + CLPGS++VTATNFCPPNNALP+++GGWC+PP HFD++QPVF RIA Y+AGIVPV
Sbjct: 329 GDRKWCLPGSILVTATNFCPPNNALPNNNGGWCSPPLQHFDLAQPVFLRIAQYKAGIVPV 508
Query: 554 SYRRVACNKKGGIRFTINGHSYFNLVLV 637
SYRRV C ++GGIRFTINGHSYFNLVLV
Sbjct: 509 SYRRVPCRRRGGIRFTINGHSYFNLVLV 592
Score = 109 bits (232), Expect(2) = 7e-028
Identities = 52/88 (59%), Positives = 58/88 (65%)
Frame = -2 / -2
Query: 636 TSTRLK*EWPLMVKRMPPFLLHATLR*ETGTMPAL*SAMRWNTGCDMSKWARGGLHQPPS 457
T T+LK*E PL+V +PP LL TL *ETGT+PAL A+ TG SK +GGLH PP
Sbjct: 591 TRTKLK*ECPLIVNLIPPLLLQGTLL*ETGTIPALY*AILRKTG*ARSKCCKGGLHHPPL 412
Query: 456 SXGSALLGGQKLVAVTTTEPGRHERPPP 373
G AL GGQKLVAVT EPGRH P
Sbjct: 411 LLGKALFGGQKLVAVTKIEPGRHHLRSP 328
Score = 107 bits (229), Expect(2) = 2e-072
Identities = 41/61 (67%), Positives = 45/61 (73%)
Frame = +2 / +2
Query: 179 GGAWSSAHATFYXXXXXXXXXXXXXXYGNLYSQGYGTNTAALSTALFNNGLSCGACFEVR 358
GG W +AHATFY YGNLYSQGYGTNTAALSTALFN+GLSCG+CFE+R
Sbjct: 143 GGGWVNAHATFYGGSDASGTMGGACGYGNLYSQGYGTNTAALSTALFNSGLSCGSCFEIR 322
Query: 359 C 361
C
Sbjct: 323 C 325
Score = 29.9 bits (59), Expect(2) = 7e-028
Identities = 15/36 (41%), Positives = 17/36 (47%)
Frame = -2 / -2
Query: 363 SHRTSKQAPQLRPXXXXXXXXXXXXVPYPWL*RLPY 256
+H SKQ PQL+P VP P L R PY
Sbjct: 327 AHLISKQEPQLKPLLNKAVLKAAVFVP*P*LYRFPY 220
>gb|CO514640.1|CO514640 s13dSG46C0600050_327940 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 536
Score = 138 bits (296), Expect(3) = 1e-065
Identities = 52/65 (80%), Positives = 58/65 (89%)
Frame = +2 / +3
Query: 461 GGWCNPPRAHFDMSQPVFQRIALYRAGIVPVSYRRVACNKKGGIRFTINGHSYFNLVLVT 640
GGWC+PP HFD+SQP+FQ IA YRAGIVPV+YRRV C ++GGIRFTINGHSYFNLVLVT
Sbjct: 342 GGWCDPPNHHFDLSQPIFQHIAQYRAGIVPVAYRRVRCKRRGGIRFTINGHSYFNLVLVT 521
Query: 641 NVEGA 655
NV GA
Sbjct: 522 NVGGA 536
Score = 105 bits (224), Expect(3) = 1e-065
Identities = 40/62 (64%), Positives = 45/62 (72%)
Frame = +2 / +3
Query: 176 GGGAWSSAHATFYXXXXXXXXXXXXXXYGNLYSQGYGTNTAALSTALFNNGLSCGACFEV 355
G +W +AHATFY YGNLYSQGYGTNTAALSTALFNNGLSCGAC+E+
Sbjct: 90 GHASWINAHATFYGGSDASGTMGGACGYGNLYSQGYGTNTAALSTALFNNGLSCGACYEI 269
Query: 356 RC 361
+C
Sbjct: 270 KC 275
Score = 77.1 bits (162), Expect(3) = 8e-024
Identities = 37/60 (61%), Positives = 41/60 (68%)
Frame = -2 / -2
Query: 639 VTSTRLK*EWPLMVKRMPPFLLHATLR*ETGTMPAL*SAMRWNTGCDMSKWARGGLHQPP 460
VT T+LK*E PLMV +PP LLH TL * TGT+PAL AM G + SKW GG HQPP
Sbjct: 520 VTRTKLK*ECPLMVNLIPPLLLHLTLL*ATGTIPALY*AMC*KIG*ERSKW*LGGSHQPP 341
Score = 40.5 bits (82), Expect(3) = 1e-065
Identities = 13/16 (81%), Positives = 15/16 (93%)
Frame = +2 / +3
Query: 389 CLPGSVVVTATNFCPP 436
C PGS++VTATNFCPP
Sbjct: 294 CNPGSIIVTATNFCPP 341
Score = 34.5 bits (69), Expect(3) = 8e-024
Identities = 16/36 (44%), Positives = 17/36 (47%)
Frame = -2 / -2
Query: 363 SHRTSKQAPQLRPXXXXXXXXXXXXVPYPWL*RLPY 256
+H S APQL P VP PWL*R PY
Sbjct: 277 THLIS*HAPQLNPLLNSAVLNAAVFVP*PWL*RFPY 170
Score = 31.3 bits (62), Expect(3) = 8e-024
Identities = 12/16 (75%), Positives = 13/16 (81%)
Frame = -2 / -2
Query: 435 GGQKLVAVTTTEPGRH 388
GGQKLVAVT +PG H
Sbjct: 340 GGQKLVAVTIIDPGLH 293
>gb|CO515743.1|CO515743 s13dSG77C1100086_419635 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 538
Score = 109 bits (232), Expect(2) = 3e-050
Identities = 38/56 (67%), Positives = 48/56 (85%)
Frame = +2 / +1
Query: 383 RSCLPGSVVVTATNFCPPNNALPSDDGGWCNPPRAHFDMSQPVFQRIALYRAGIVP 550
R C PGS++VTATNFCPPN +LP+++GGWCNPP HFDM++P + +IA YRAGIVP
Sbjct: 370 RWCKPGSIIVTATNFCPPNPSLPNNNGGWCNPPLQHFDMAEPAYLQIAEYRAGIVP 537
Score = 105 bits (223), Expect(2) = 3e-050
Identities = 40/61 (65%), Positives = 45/61 (73%)
Frame = +2 / +1
Query: 182 GAWSSAHATFYXXXXXXXXXXXXXXYGNLYSQGYGTNTAALSTALFNNGLSCGACFEVRC 361
G W SAHATFY YGNLYSQGYGTNTAALSTALFN+GLSCG+C+E+RC
Sbjct: 178 GGWESAHATFYGGGDASGTMGGACGYGNLYSQGYGTNTAALSTALFNSGLSCGSCYEMRC 357
Query: 362 D 364
+
Sbjct: 358 N 360
>gb|CO514666.1|CO514666 s13dSG46E1100087_327992 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 542
Score = 111 bits (238), Expect(2) = 5e-050
Identities = 43/62 (69%), Positives = 46/62 (74%)
Frame = +2 / +1
Query: 179 GGAWSSAHATFYXXXXXXXXXXXXXXYGNLYSQGYGTNTAALSTALFNNGLSCGACFEVR 358
GG W+SAHATFY YGNLYSQGYG NTAALSTALFNNGLSCGACFE++
Sbjct: 169 GGPWTSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNNGLSCGACFELK 348
Query: 359 CD 364
CD
Sbjct: 349 CD 354
Score = 101 bits (215), Expect(2) = 5e-050
Identities = 36/51 (70%), Positives = 44/51 (86%)
Frame = +2 / +1
Query: 401 SVVVTATNFCPPNNALPSDDGGWCNPPRAHFDMSQPVFQRIALYRAGIVPV 553
S+++TATNFCPPN A PSD+GGWCNPPR H D++ P+F +IA YRAGIVPV
Sbjct: 388 SILITATNFCPPNFAQPSDNGGWCNPPRPHSDLAMPMFLKIAQYRAGIVPV 540
>gb|CO515120.1|CO515120 s13dSG40E0200019_399105 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 314
Score = 100 bits (214), Expect(2) = 5e-037
Identities = 37/56 (66%), Positives = 46/56 (82%)
Frame = +2 / +3
Query: 701 WQALSRNWGQNWQSNTLLDGQALSFRVTTSDGRSVLSNNAAPRGWSFGQTFSGAQF 868
WQA+SRNWGQNWQSN+ L+GQ+LS VTT +G S++S N AP WSFGQT++G QF
Sbjct: 108 WQAMSRNWGQNWQSNSYLNGQSLSVVVTTGNGHSIVSFNVAPPSWSFGQTYTGRQF 275
Score = 68.9 bits (144), Expect(2) = 5e-037
Identities = 27/33 (81%), Positives = 30/33 (90%)
Frame = +2 / +3
Query: 596 FTINGHSYFNLVLVTNVEGAGDVHAVAVKADRS 694
FTINGHSYFNLVLVTNV GAGDVH+VA+K R+
Sbjct: 6 FTINGHSYFNLVLVTNVGGAGDVHSVAIKGSRT 104
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,281,693
Number of Sequences: 7669
Number of extensions: 121494
Number of successful extensions: 1035
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 351696952
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)