TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493944.2.2
(2027 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF537205.1| Medicago sativa apoplastic invertase-like mR... 136 1e-061
>gb|AF537205.1| Medicago sativa apoplastic invertase-like mRNA, partial sequence
Length = 714
Score = 136 bits (292), Expect(5) = 1e-061
Identities = 51/86 (59%), Positives = 64/86 (74%)
Frame = +1 / +1
Query: 976 DEHRLRYDYGNFYASKTFYDPAKRRRILWGWANESDTAADDVAKGWAGIQAIPRTVWLDP 1155
+++ LRYDYG +YASKTF+D K RRIL GWANES + DDV KGW+GI IPR +WL
Sbjct: 82 NQNVLRYDYGKYYASKTFFDDGKNRRILLGWANESSSVVDDVKKGWSGIHTIPRAIWLHK 261
Query: 1156 SGKQLLQWPIEEVEALRAKSVTLKNR 1233
SGKQL+QWP++E+E LR V N+
Sbjct: 262 SGKQLVQWPVKELENLRMNPVNWPNQ 339
Score = 75.3 bits (158), Expect(3) = 2e-027
Identities = 35/76 (46%), Positives = 44/76 (57%)
Frame = -1 / -2
Query: 1229 FLRVTDFALSASTSSIGHCNSCFPLGSSHTVLGMAWIPAQPLATSSAAVSDSLAQPHRMR 1050
F ++T F S+S GHC +CFP S LG+ WIP P TSS + DSLAQP+++
Sbjct: 335 FGQLTGFIRRFSSSFTGHCTNCFPDL*SQIALGIVWIPDHPFLTSSTTLEDSLAQPNKIL 156
Query: 1049 RRLAGS*NVLDA*KLP 1002
S NVLDA* P
Sbjct: 155 LFFPSSKNVLDA*YFP 108
Score = 62.0 bits (129), Expect(5) = 1e-061
Identities = 26/42 (61%), Positives = 31/42 (73%)
Frame = +1 / +2
Query: 1363 DAERLCGVKRADVRGGVGPFGLWVLASANRKERTAVFFRVFK 1488
D + LC K A V+GGVGPFGL VLAS +E TAVFFR+F+
Sbjct: 458 DPQILCSQKGASVKGGVGPFGLHVLASKGLQEYTAVFFRIFR 583
Score = 53.3 bits (110), Expect(3) = 2e-027
Identities = 24/37 (64%), Positives = 25/37 (67%)
Frame = -1 / -1
Query: 1487 LNTLKNTAVLSLRLAEASTHSPNGPTPPLTSARFTPH 1377
LN LKNTAV S + EAST PNGPTPP T A F H
Sbjct: 582 LNILKNTAVYSCKPLEASTCRPNGPTPPFTDAPF*LH 472
Score = 41.8 bits (85), Expect(5) = 1e-061
Identities = 15/29 (51%), Positives = 22/29 (75%)
Frame = +1 / +2
Query: 1225 KNRVIKAGHHVEVTGIQTAQADVEVSFEV 1311
+ +VIK G + +TG+ + QADVE+SFEV
Sbjct: 332 QTKVIKGGELIPITGVNSVQADVEISFEV 418
Score = 39.6 bits (80), Expect(5) = 1e-061
Identities = 18/40 (45%), Positives = 25/40 (62%)
Frame = +1 / +2
Query: 1504 KPVVLMCTDPTKSSQNPNLYRPTFAGFVDTDISNGKISLR 1623
K +VLMC+D +K+S N T+ FVD D + K+SLR
Sbjct: 593 KNLVLMCSDQSKTSLNKENDMTTYGTFVDVDPLHEKLSLR 712
Score = 27.6 bits (54), Expect(3) = 2e-027
Identities = 14/27 (51%), Positives = 14/27 (51%)
Frame = -1 / -1
Query: 1310 TSKLTSTSACAVCIPVTST*CPALITL 1230
TS STSAC PV P LITL
Sbjct: 417 TSNEISTSACTELTPVIGINSPPLITL 337
Score = 27.6 bits (54), Expect(5) = 1e-061
Identities = 9/29 (31%), Positives = 17/29 (58%)
Frame = +1 / +1
Query: 904 RYDYYTVGTYHPRAERYVPDDPAGDEHRL 990
++D+Y +GTY + +VPD+ D +
Sbjct: 7 KHDHYLIGTYDTVKDVFVPDNGFEDNQNV 93
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,121,110
Number of Sequences: 7669
Number of extensions: 166100
Number of successful extensions: 1490
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 45
effective length of database: 903,463
effective search space used: 569181690
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)