TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493531.2.1
(1061 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514640.1|CO514640 s13dSG46C0600050_327940 Glandular tr... 89 3e-034
gb|CO515120.1|CO515120 s13dSG40E0200019_399105 Glandular tr... 125 1e-029
gb|CO513205.1|CO513205 s13dSG24B0800061_129574 Glandular tr... 80 2e-024
>gb|CO514640.1|CO514640 s13dSG46C0600050_327940 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 536
Score = 88.6 bits (187), Expect(3) = 3e-034
Identities = 32/59 (54%), Positives = 39/59 (66%)
Frame = +2 / +3
Query: 227 YYAADPEDAIGGACGFGDLGKHGYGMATVGLSTALFERGAACGGCYEVKCVEDLKHCNP 403
Y +D +GGACG+G+L GYG T LSTALF G +CG CYE+KCV D + CNP
Sbjct: 126 YGGSDASGTMGGACGYGNLYSQGYGTNTAALSTALFNNGLSCGACYEIKCVNDPQWCNP 302
Score = 62.9 bits (131), Expect(3) = 3e-034
Identities = 21/38 (55%), Positives = 31/38 (81%)
Frame = +2 / +3
Query: 419 LMPIQYRRVKCLREGGVRFSVNGRHFFFTVLISNVGGA 532
++P+ YRRV+C R GG+RF++NG +F VL++NVGGA
Sbjct: 423 IVPVAYRRVRCKRRGGIRFTINGHSYFNLVLVTNVGGA 536
Score = 26.7 bits (52), Expect(3) = 3e-034
Identities = 7/8 (87%), Positives = 8/8 (100%)
Frame = +2 / +3
Query: 395 CNPPNHHF 418
C+PPNHHF
Sbjct: 351 CDPPNHHF 374
>gb|CO515120.1|CO515120 s13dSG40E0200019_399105 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 314
Score = 125 bits (267), Expect = 1e-029
Identities = 47/91 (51%), Positives = 62/91 (68%)
Frame = +2 / +3
Query: 473 FSVNGRHFFFTVLISNVGGAGDVRSVKIKGTELGWLPMGRNWGQVWHINCDMRGQPLSFE 652
F++NG +F VL++NVGGAGDV SV IKG+ W M RNWGQ W N + GQ LS
Sbjct: 6 FTINGHSYFNLVLVTNVGGAGDVHSVAIKGSRTRWQAMSRNWGQNWQSNSYLNGQSLSVV 185
Query: 653 LSSSDSKTLTSFNVVPKDWEFGKTYTGKQFL 745
+++ + ++ SFNV P W FG+TYTG+QFL
Sbjct: 186 VTTGNGHSIVSFNVAPPSWSFGQTYTGRQFL 278
>gb|CO513205.1|CO513205 s13dSG24B0800061_129574 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 592
Score = 80.3 bits (169), Expect(2) = 2e-024
Identities = 29/59 (49%), Positives = 37/59 (62%)
Frame = +2 / +2
Query: 227 YYAADPEDAIGGACGFGDLGKHGYGMATVGLSTALFERGAACGGCYEVKCVEDLKHCNP 403
Y +D +GGACG+G+L GYG T LSTALF G +CG C+E++C D K C P
Sbjct: 176 YGGSDASGTMGGACGYGNLYSQGYGTNTAALSTALFNSGLSCGSCFEIRCAGDRKWCLP 352
Score = 49.6 bits (102), Expect(2) = 2e-024
Identities = 16/32 (50%), Positives = 24/32 (75%)
Frame = +2 / +2
Query: 419 LMPIQYRRVKCLREGGVRFSVNGRHFFFTVLI 514
++P+ YRRV C R GG+RF++NG +F VL+
Sbjct: 497 IVPVSYRRVPCRRRGGIRFTINGHSYFNLVLV 592
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,843,375
Number of Sequences: 7669
Number of extensions: 103309
Number of successful extensions: 1152
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 284828310
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)