TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2485939.2.1
(530 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512415.1|CO512415 s13dSG80F0600047_114344 Glandular tr... 163 3e-041
gb|CO511707.1|CO511707 s13dSG01B0800073_103314 Glandular tr... 160 2e-040
gb|CO515771.1|CO515771 s13dSG77F1000091_419691 Glandular tr... 142 8e-035
gb|CO514833.1|CO514833 s13dSG59G1100088_328326 Glandular tr... 111 4e-030
gb|CO513208.1|CO513208 s13dSG24B1200093_129580 Glandular tr... 111 3e-029
>gb|CO512415.1|CO512415 s13dSG80F0600047_114344 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 565
Score = 163 bits (351), Expect = 3e-041
Identities = 61/108 (56%), Positives = 78/108 (72%)
Frame = +3 / +2
Query: 126 KPNYRKGLWSPEEDQKLRDYILLHGHGCWSALPAKAGLQRNGKSCRLRWINYLRPGLKHG 305
K ++G W+ EE + L YI +G G W +LP AGL R GKSCRLRWINYLR LK G
Sbjct: 188 KVGLKRGRWTTEEGEILSKYIQANGEGSWRSLPKNAGLLRCGKSCRLRWINYLRTDLKRG 367
Query: 306 MFSPEEEETVMSLHATLGNKWSRIARHLPGRTDNEVKNYWNSYLKKRV 449
S +EE+T++ LH T GN+WS IA HLPGRTDNE+KN+WNS+L +++
Sbjct: 368 NISAQEEDTIVKLHTTYGNRWSMIASHLPGRTDNEIKNHWNSHLSRKI 511
>gb|CO511707.1|CO511707 s13dSG01B0800073_103314 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 570
Score = 160 bits (345), Expect = 2e-040
Identities = 61/114 (53%), Positives = 80/114 (70%)
Frame = +3 / +1
Query: 126 KPNYRKGLWSPEEDQKLRDYILLHGHGCWSALPAKAGLQRNGKSCRLRWINYLRPGLKHG 305
K +KG W+PEED L YI HG G W A+P K GL R KSCRLRW NYLRPG+K G
Sbjct: 37 KEGVKKGPWTPEEDIILVTYIQEHGPGNWRAVPTKTGLSRCSKSCRLRWTNYLRPGIKRG 216
Query: 306 MFSPEEEETVMSLHATLGNKWSRIARHLPGRTDNEVKNYWNSYLKKRVEGKDQG 467
F+ +EE+ ++ L LGN+W+ IA +LP RTDN++KNYWN++LKK+++ G
Sbjct: 217 NFTEQEEKMIIHLQDLLGNRWAAIASYLPQRTDNDIKNYWNTHLKKKLKKSQTG 378
>gb|CO515771.1|CO515771 s13dSG77F1000091_419691 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 413
Score = 142 bits (304), Expect = 8e-035
Identities = 55/104 (52%), Positives = 72/104 (69%)
Frame = +3 / +3
Query: 138 RKGLWSPEEDQKLRDYILLHGHGCWSALPAKAGLQRNGKSCRLRWINYLRPGLKHGMFSP 317
RKG W+ EED L+ I +G G W +P +AGL R KSCRLRW+NYL P +K FS
Sbjct: 96 RKGAWTYEEDNLLKACINKYGEGKWHLVPKRAGLNRCRKSCRLRWVNYLNPAIKRESFSH 275
Query: 318 EEEETVMSLHATLGNKWSRIARHLPGRTDNEVKNYWNSYLKKRV 449
+E + ++ LH LGN+WS IA LPGRT N+VKNYW++ L+K+V
Sbjct: 276 DEIDMILRLHKLLGNRWSLIAARLPGRTANDVKNYWHTNLRKKV 407
>gb|CO514833.1|CO514833 s13dSG59G1100088_328326 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 480
Score = 111 bits (236), Expect(2) = 4e-030
Identities = 40/68 (58%), Positives = 53/68 (77%)
Frame = +3 / +3
Query: 243 RNGKSCRLRWINYLRPGLKHGMFSPEEEETVMSLHATLGNKWSRIARHLPGRTDNEVKNY 422
R+GKSCRLRW N L P ++H F+PEE++T++ HA GNKW+ IAR L GRTDN +KN+
Sbjct: 153 RSGKSCRLRWCNQLSPQVEHRAFTPEEDDTIIRAHARFGNKWATIARLLSGRTDNAIKNH 332
Query: 423 WNSYLKKR 446
WNS LK++
Sbjct: 333 WNSTLKRK 356
Score = 35.4 bits (71), Expect(2) = 4e-030
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +3 / +3
Query: 141 KGLWSPEEDQKLRDYILLHGHGCWSAL 221
KG WSPEED+ L+ + HG WS +
Sbjct: 54 KGPWSPEEDEALQKLVEKHGPRNWSII 134
>gb|CO513208.1|CO513208 s13dSG24B1200093_129580 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 598
Score = 111 bits (236), Expect(2) = 3e-029
Identities = 40/68 (58%), Positives = 53/68 (77%)
Frame = +3 / +3
Query: 243 RNGKSCRLRWINYLRPGLKHGMFSPEEEETVMSLHATLGNKWSRIARHLPGRTDNEVKNY 422
R+GKSCRLRW N L P ++H F+PEE++T++ HA GNKW+ IAR L GRTDN +KN+
Sbjct: 255 RSGKSCRLRWCNQLSPQVEHRAFTPEEDDTIIRAHARFGNKWATIARLLSGRTDNAIKNH 434
Query: 423 WNSYLKKR 446
WNS LK++
Sbjct: 435 WNSTLKRK 458
Score = 32.2 bits (64), Expect(2) = 3e-029
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = +3 / +3
Query: 87 HSPAMGCKACDKPKPNYRKGLWSPEEDQKLRDYILLHGHGCWSAL 221
H+ M ++ + + KG WSPEED+ L+ + H WS +
Sbjct: 102 HNLHMNNTVINRKEMDRIKGPWSPEEDEALQKLVEKHRPRNWSII 236
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,383,174
Number of Sequences: 7669
Number of extensions: 38823
Number of successful extensions: 1178
Number of sequences better than 1.0e-020: 6
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 126108765
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)