TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2455940.2.1
(1115 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U20736.1|MSU20736 Medicago sativa S-adenosyl-L-methionin... 465 e-132
emb|AX259371.1| Sequence 1 from Patent WO0173090 465 e-132
emb|CQ760958.1| Sequence 3 from Patent WO2004002216 465 e-132
emb|CQ760964.1| Sequence 9 from Patent WO2004002216 465 e-132
gb|DQ122814.1| Medicago sativa clone C31 mRNA sequence 219 6e-058
>gb|U20736.1|MSU20736 Medicago sativa S-adenosyl-L-methionine:trans-caffeoyl-CoA
3-O-methyltransferase (CCOMT) mRNA, complete cds
Length = 966
Score = 465 bits (1010), Expect = e-132
Identities = 186/235 (79%), Positives = 211/235 (89%)
Frame = -2 / +3
Query: 955 RHSEVGHKSLLKSDDLYQYILDTSVYPREPESMKELREITAKHPWNLMTTSADEGQFLNM 776
RH EVGHKSLL+SD LYQYIL+TSV+PRE E+MKELRE+TAKHPWN+MTTSADEGQFL+M
Sbjct: 75 RHQEVGHKSLLQSDALYQYILETSVFPREHEAMKELREVTAKHPWNIMTTSADEGQFLSM 254
Query: 775 LIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILAMDINRENYELGLPCIEKAGVAHKI 596
L+KLI AK TMEIGVYTGYSLLATALA+PEDG ILAMDIN+ENYELGLP I+KAGV HKI
Sbjct: 255 LLKLINAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINKENYELGLPVIKKAGVDHKI 434
Query: 595 DFREGPALPVLDDLIAEEKNHGSFDFVFVDADKDNYLNYHERXXXXXXXXXLIGYDNTLW 416
DFREGPALPVLD++I +EKNHGS+DF+FVDADKDNYLNYH+R +IGYDNTLW
Sbjct: 435 DFREGPALPVLDEMIKDEKNHGSYDFIFVDADKDNYLNYHKRLIDLVKVGGVIGYDNTLW 614
Query: 415 NGSVVLPDDAPMRKYIRFYRDFVLVLNKALAADDRVEICQLPVGDGVTLCRRVK* 251
NGSVV P DAP+RKY+R+YRDFVL LNKALA D R+EIC LPVGDG+T+CRR+K*
Sbjct: 615 NGSVVAPPDAPLRKYVRYYRDFVLELNKALAVDPRIEICMLPVGDGITICRRIK* 779
Score = 196 bits (422), Expect(2) = 1e-060
Identities = 92/125 (73%), Positives = 102/125 (81%)
Frame = +3 / -3
Query: 531 PWFFSSAMRSSRTGSAGPSRKSILWATPAFSMQGRPSS*FSRLMSMAKIVPSSGRASAVA 710
P FFSS + SSRTG AGPS KSIL +TPAF + GRP+S*FS LMS+AKI PSSG A AVA
Sbjct: 499 P*FFSSLIISSRTGRAGPSLKSIL*STPAFLITGRPNS*FSLLMSIAKIFPSSGIARAVA 320
Query: 711 RRE*PV*TPISMVFLAPMSLMSMFRNCPSSAEVVIRFHGCLAVISRSSFMLSGSRGYTLV 890
RRE*PV*TPISMVFLA +SL SM +NCPSSAEVV+ FHGC AV S +SFM S S G TLV
Sbjct: 319 RRE*PV*TPISMVFLALISLRSMLKNCPSSAEVVMMFHGCFAVTSLNSFMASCSLGKTLV 140
Query: 891 SRMYW 905
SR+YW
Sbjct: 139 SRIYW 125
Score = 52.4 bits (108), Expect(2) = 1e-060
Identities = 26/59 (44%), Positives = 29/59 (49%)
Frame = +3 / -3
Query: 252 HLTRRQRVTPSPTGSWQIXXXXXXXXXXXXXXXKSR*KRMYLRMGASSGSTTEPFHSVL 428
+L RQ V PSPTGS I KS * Y GAS G+TT+PFH VL
Sbjct: 778 YLILRQIVIPSPTGSIHISILGSTAKALLSSKTKSL*YLTYFLNGASGGATTDPFHKVL 602
>emb|AX259371.1| Sequence 1 from Patent WO0173090
Length = 744
Score = 465 bits (1010), Expect = e-132
Identities = 186/235 (79%), Positives = 211/235 (89%)
Frame = -2 / +1
Query: 955 RHSEVGHKSLLKSDDLYQYILDTSVYPREPESMKELREITAKHPWNLMTTSADEGQFLNM 776
RH EVGHKSLL+SD LYQYIL+TSV+PRE E+MKELRE+TAKHPWN+MTTSADEGQFL+M
Sbjct: 40 RHQEVGHKSLLQSDALYQYILETSVFPREHEAMKELREVTAKHPWNIMTTSADEGQFLSM 219
Query: 775 LIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILAMDINRENYELGLPCIEKAGVAHKI 596
L+KLI AK TMEIGVYTGYSLLATALA+PEDG ILAMDIN+ENYELGLP I+KAGV HKI
Sbjct: 220 LLKLINAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINKENYELGLPVIKKAGVDHKI 399
Query: 595 DFREGPALPVLDDLIAEEKNHGSFDFVFVDADKDNYLNYHERXXXXXXXXXLIGYDNTLW 416
DFREGPALPVLD++I +EKNHGS+DF+FVDADKDNYLNYH+R +IGYDNTLW
Sbjct: 400 DFREGPALPVLDEMIKDEKNHGSYDFIFVDADKDNYLNYHKRLIDLVKVGGVIGYDNTLW 579
Query: 415 NGSVVLPDDAPMRKYIRFYRDFVLVLNKALAADDRVEICQLPVGDGVTLCRRVK* 251
NGSVV P DAP+RKY+R+YRDFVL LNKALA D R+EIC LPVGDG+T+CRR+K*
Sbjct: 580 NGSVVAPPDAPLRKYVRYYRDFVLELNKALAVDPRIEICMLPVGDGITICRRIK* 744
Score = 196 bits (422), Expect(2) = 1e-060
Identities = 92/125 (73%), Positives = 102/125 (81%)
Frame = +3 / -2
Query: 531 PWFFSSAMRSSRTGSAGPSRKSILWATPAFSMQGRPSS*FSRLMSMAKIVPSSGRASAVA 710
P FFSS + SSRTG AGPS KSIL +TPAF + GRP+S*FS LMS+AKI PSSG A AVA
Sbjct: 464 P*FFSSLIISSRTGRAGPSLKSIL*STPAFLITGRPNS*FSLLMSIAKIFPSSGIARAVA 285
Query: 711 RRE*PV*TPISMVFLAPMSLMSMFRNCPSSAEVVIRFHGCLAVISRSSFMLSGSRGYTLV 890
RRE*PV*TPISMVFLA +SL SM +NCPSSAEVV+ FHGC AV S +SFM S S G TLV
Sbjct: 284 RRE*PV*TPISMVFLALISLRSMLKNCPSSAEVVMMFHGCFAVTSLNSFMASCSLGKTLV 105
Query: 891 SRMYW 905
SR+YW
Sbjct: 104 SRIYW 90
Score = 52.4 bits (108), Expect(2) = 1e-060
Identities = 26/59 (44%), Positives = 29/59 (49%)
Frame = +3 / -2
Query: 252 HLTRRQRVTPSPTGSWQIXXXXXXXXXXXXXXXKSR*KRMYLRMGASSGSTTEPFHSVL 428
+L RQ V PSPTGS I KS * Y GAS G+TT+PFH VL
Sbjct: 743 YLILRQIVIPSPTGSIHISILGSTAKALLSSKTKSL*YLTYFLNGASGGATTDPFHKVL 567
>emb|CQ760958.1| Sequence 3 from Patent WO2004002216
Length = 744
Score = 465 bits (1010), Expect = e-132
Identities = 186/235 (79%), Positives = 211/235 (89%)
Frame = -2 / +1
Query: 955 RHSEVGHKSLLKSDDLYQYILDTSVYPREPESMKELREITAKHPWNLMTTSADEGQFLNM 776
RH EVGHKSLL+SD LYQYIL+TSV+PRE E+MKELRE+TAKHPWN+MTTSADEGQFL+M
Sbjct: 40 RHQEVGHKSLLQSDALYQYILETSVFPREHEAMKELREVTAKHPWNIMTTSADEGQFLSM 219
Query: 775 LIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILAMDINRENYELGLPCIEKAGVAHKI 596
L+KLI AK TMEIGVYTGYSLLATALA+PEDG ILAMDIN+ENYELGLP I+KAGV HKI
Sbjct: 220 LLKLINAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINKENYELGLPVIKKAGVDHKI 399
Query: 595 DFREGPALPVLDDLIAEEKNHGSFDFVFVDADKDNYLNYHERXXXXXXXXXLIGYDNTLW 416
DFREGPALPVLD++I +EKNHGS+DF+FVDADKDNYLNYH+R +IGYDNTLW
Sbjct: 400 DFREGPALPVLDEMIKDEKNHGSYDFIFVDADKDNYLNYHKRLIDLVKVGGVIGYDNTLW 579
Query: 415 NGSVVLPDDAPMRKYIRFYRDFVLVLNKALAADDRVEICQLPVGDGVTLCRRVK* 251
NGSVV P DAP+RKY+R+YRDFVL LNKALA D R+EIC LPVGDG+T+CRR+K*
Sbjct: 580 NGSVVAPPDAPLRKYVRYYRDFVLELNKALAVDPRIEICMLPVGDGITICRRIK* 744
Score = 196 bits (422), Expect(2) = 1e-060
Identities = 92/125 (73%), Positives = 102/125 (81%)
Frame = +3 / -2
Query: 531 PWFFSSAMRSSRTGSAGPSRKSILWATPAFSMQGRPSS*FSRLMSMAKIVPSSGRASAVA 710
P FFSS + SSRTG AGPS KSIL +TPAF + GRP+S*FS LMS+AKI PSSG A AVA
Sbjct: 464 P*FFSSLIISSRTGRAGPSLKSIL*STPAFLITGRPNS*FSLLMSIAKIFPSSGIARAVA 285
Query: 711 RRE*PV*TPISMVFLAPMSLMSMFRNCPSSAEVVIRFHGCLAVISRSSFMLSGSRGYTLV 890
RRE*PV*TPISMVFLA +SL SM +NCPSSAEVV+ FHGC AV S +SFM S S G TLV
Sbjct: 284 RRE*PV*TPISMVFLALISLRSMLKNCPSSAEVVMMFHGCFAVTSLNSFMASCSLGKTLV 105
Query: 891 SRMYW 905
SR+YW
Sbjct: 104 SRIYW 90
Score = 52.4 bits (108), Expect(2) = 1e-060
Identities = 26/59 (44%), Positives = 29/59 (49%)
Frame = +3 / -2
Query: 252 HLTRRQRVTPSPTGSWQIXXXXXXXXXXXXXXXKSR*KRMYLRMGASSGSTTEPFHSVL 428
+L RQ V PSPTGS I KS * Y GAS G+TT+PFH VL
Sbjct: 743 YLILRQIVIPSPTGSIHISILGSTAKALLSSKTKSL*YLTYFLNGASGGATTDPFHKVL 567
>emb|CQ760964.1| Sequence 9 from Patent WO2004002216
Length = 1906
Score = 465 bits (1010), Expect = e-132
Identities = 186/235 (79%), Positives = 211/235 (89%)
Frame = -2 / +1
Query: 955 RHSEVGHKSLLKSDDLYQYILDTSVYPREPESMKELREITAKHPWNLMTTSADEGQFLNM 776
RH EVGHKSLL+SD LYQYIL+TSV+PRE E+MKELRE+TAKHPWN+MTTSADEGQFL+M
Sbjct: 1015 RHQEVGHKSLLQSDALYQYILETSVFPREHEAMKELREVTAKHPWNIMTTSADEGQFLSM 1194
Query: 775 LIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILAMDINRENYELGLPCIEKAGVAHKI 596
L+KLI AK TMEIGVYTGYSLLATALA+PEDG ILAMDIN+ENYELGLP I+KAGV HKI
Sbjct: 1195 LLKLINAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINKENYELGLPVIKKAGVDHKI 1374
Query: 595 DFREGPALPVLDDLIAEEKNHGSFDFVFVDADKDNYLNYHERXXXXXXXXXLIGYDNTLW 416
DFREGPALPVLD++I +EKNHGS+DF+FVDADKDNYLNYH+R +IGYDNTLW
Sbjct: 1375 DFREGPALPVLDEMIKDEKNHGSYDFIFVDADKDNYLNYHKRLIDLVKVGGVIGYDNTLW 1554
Query: 415 NGSVVLPDDAPMRKYIRFYRDFVLVLNKALAADDRVEICQLPVGDGVTLCRRVK* 251
NGSVV P DAP+RKY+R+YRDFVL LNKALA D R+EIC LPVGDG+T+CRR+K*
Sbjct: 1555 NGSVVAPPDAPLRKYVRYYRDFVLELNKALAVDPRIEICMLPVGDGITICRRIK* 1719
Score = 196 bits (422), Expect(2) = 1e-060
Identities = 92/125 (73%), Positives = 102/125 (81%)
Frame = +3 / -3
Query: 531 PWFFSSAMRSSRTGSAGPSRKSILWATPAFSMQGRPSS*FSRLMSMAKIVPSSGRASAVA 710
P FFSS + SSRTG AGPS KSIL +TPAF + GRP+S*FS LMS+AKI PSSG A AVA
Sbjct: 1439 P*FFSSLIISSRTGRAGPSLKSIL*STPAFLITGRPNS*FSLLMSIAKIFPSSGIARAVA 1260
Query: 711 RRE*PV*TPISMVFLAPMSLMSMFRNCPSSAEVVIRFHGCLAVISRSSFMLSGSRGYTLV 890
RRE*PV*TPISMVFLA +SL SM +NCPSSAEVV+ FHGC AV S +SFM S S G TLV
Sbjct: 1259 RRE*PV*TPISMVFLALISLRSMLKNCPSSAEVVMMFHGCFAVTSLNSFMASCSLGKTLV 1080
Query: 891 SRMYW 905
SR+YW
Sbjct: 1079 SRIYW 1065
Score = 52.4 bits (108), Expect(2) = 1e-060
Identities = 26/59 (44%), Positives = 29/59 (49%)
Frame = +3 / -3
Query: 252 HLTRRQRVTPSPTGSWQIXXXXXXXXXXXXXXXKSR*KRMYLRMGASSGSTTEPFHSVL 428
+L RQ V PSPTGS I KS * Y GAS G+TT+PFH VL
Sbjct: 1718 YLILRQIVIPSPTGSIHISILGSTAKALLSSKTKSL*YLTYFLNGASGGATTDPFHKVL 1542
>gb|DQ122814.1| Medicago sativa clone C31 mRNA sequence
Length = 562
Score = 219 bits (472), Expect = 6e-058
Identities = 84/143 (58%), Positives = 111/143 (77%)
Frame = -2 / +2
Query: 928 LLKSDDLYQYILDTSVYPREPESMKELREITAKHPWNLMTTSADEGQFLNMLIKLIGAKK 749
+L+S+DL +YILDT+VYPREPE +KELR T HPW + T + GQ + +L+KL+ KK
Sbjct: 134 ILQSEDLTKYILDTAVYPREPEPLKELRNATENHPWGFIATLPEAGQLITLLLKLLNPKK 313
Query: 748 TMEIGVYTGYSLLATALALPEDGTILAMDINRENYELGLPCIEKAGVAHKIDFREGPALP 569
T+E+GV+TGYSLL TAL +P+DG I A+DI+R+ YE+GLP I KAGV HKID+ E PALP
Sbjct: 314 TIEVGVFTGYSLLLTALNIPDDGKITAIDIDRKTYEIGLPVIRKAGVEHKIDYIESPALP 493
Query: 568 VLDDLIAEEKNHGSFDFVFVDAD 500
+LD L+ + N G+FDF F+DAD
Sbjct: 494 ILDKLLEDPTNEGTFDFAFIDAD 562
Score = 101 bits (216), Expect = 1e-022
Identities = 60/127 (47%), Positives = 73/127 (57%)
Frame = +3 / -2
Query: 525 NDPWFFSSAMRSSRTGSAGPSRKSILWATPAFSMQGRPSS*FSRLMSMAKIVPSSGRASA 704
N P F S+ S+ G AG S SIL +TPAF M G P S* +SMA I PSSG SA
Sbjct: 537 NVPSFVGSSSSLSKIGRAGDSM*SILCSTPAFLMTGSPIS*VFLSISMAVIFPSSGMLSA 358
Query: 705 VARRE*PV*TPISMVFLAPMSLMSMFRNCPSSAEVVIRFHGCLAVISRSSFMLSGSRGYT 884
V RRE PV TP S+VFL S S + P+S +V ++ G +V SS SGS G T
Sbjct: 357 VRRREYPVNTPTSIVFLGFNSFKSRVIS*PASGKVAMKPQG*FSVAFLSSLRGSGSLG*T 178
Query: 885 LVSRMYW 905
VS +Y+
Sbjct: 177 AVSNIYF 157
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,037,285
Number of Sequences: 7669
Number of extensions: 44719
Number of successful extensions: 1315
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 301366728
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)