TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2448385.2.9
(708 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512950.1|CO512950 s13dSG86G0500036_121826 Glandular tr... 320 1e-088
>gb|CO512950.1|CO512950 s13dSG86G0500036_121826 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 600
Score = 320 bits (693), Expect = 1e-088
Identities = 134/144 (93%), Positives = 138/144 (95%)
Frame = +1 / +3
Query: 94 TMGKTRGMGAGRKLKTHRRNQRWADKAYKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAK 273
TMGKTRGMGA RKL+ R QRWADK +KKSHLGNEWKKPF+GSSHAKGIVLEKIGIEAK
Sbjct: 81 TMGKTRGMGAARKLRRLRIKQRWADKQFKKSHLGNEWKKPFSGSSHAKGIVLEKIGIEAK 260
Query: 274 QPNSAIRKCARVQLVKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRF 453
QPNSAIRKCARVQL+KNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRF
Sbjct: 261 QPNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRF 440
Query: 454 KVVKVSGVSLLALFKEKKEKPRS* 525
KVVKVSGVSLLALFKEKKEKPRS*
Sbjct: 441 KVVKVSGVSLLALFKEKKEKPRS* 512
Score = 229 bits (495), Expect = 3e-061
Identities = 104/143 (72%), Positives = 112/143 (78%)
Frame = -2 / -3
Query: 521 DLGFSFFSLKSASSXXXXXXXXXXXXPGMSPTA*PLRPNPAINTSSFSSM*FRQPSFGTK 342
DLGFSFFSLK AS PG+SPTA*PLRPNPAI TSSFSSM*F+QPSFGTK
Sbjct: 508 DLGFSFFSLKRASRETPETFTTLNLTPGISPTA*PLRPNPAIKTSSFSSM*FKQPSFGTK 329
Query: 341 AAIFFPFFTS*TRAHLRMAEFGCLASIPIFSRTMPLA*DDPAKGFFHSLPKWLFLYALSA 162
AAIFFPF +*T AHLRMAE GCLASIPIFSRT+PLA D+P KGFFHS P+ FL LSA
Sbjct: 328 AAIFFPFLIN*TLAHLRMAELGCLASIPIFSRTIPLAWDEPEKGFFHSFPR*DFLNCLSA 149
Query: 161 HLWFLRWVLSLRPAPIPRVFPMV 93
HL +R +LSLR APIP VFPMV
Sbjct: 148 HLCLIRSLLSLRAAPIPLVFPMV 80
Score = 178 bits (383), Expect = 7e-046
Identities = 79/150 (52%), Positives = 102/150 (68%)
Frame = +3 / +2
Query: 75 AGSFLRHHGEDTWYGSWAQAQDPPQEPEVG*QSIQEEPLGQ*VEKTFCWIISRQGHRSGE 254
A L HHGED GS +QA++ P + +VG* +I E +*+E+T W I G+ S +
Sbjct: 62 ADLILDHHGEDKRNGSCSQAEETPYQAKVG**AI*EISSWE*MEETLFWFIPC*GNCS*K 241
Query: 255 DWY*GQAAKFGHP*VCPCSAGEEWKEDCCLCAE*WLPKLHRGE**GVDCWIWS*GSCCGR 434
DWY*G AA+ H *VC S ++W+EDCCLC +*WL +LH GE** +D WIW+* SCC
Sbjct: 242 DWY*G*AAQLCHS*VC*GSINQKWEEDCCLCPK*WLLELH*GE**SLDRWIWT*RSCCW* 421
Query: 435 HSWCQVQGC*GVWCVAACTLQGEEGEAKVL 524
+SW QVQG G WC++ C+ QGEEGEA VL
Sbjct: 422 YSWSQVQGREGFWCLSTCSFQGEEGEA*VL 511
Score = 163 bits (351), Expect = 2e-041
Identities = 75/143 (52%), Positives = 101/143 (70%)
Frame = -1 / -2
Query: 522 RPWXXXXXLEECKQRHTRHLNNLEPDTRNVSHSMTLTTKSSNQHLIILLDVV*ATIIRHK 343
RP LE+ K R TR+L++LEPD+RN+++SMT T+KSS+Q IILL+V+ ATII K
Sbjct: 509 RPRLLLLLLEKSK*RDTRNLHDLEPDSRNITNSMTFTSKSSDQDFIILLNVIQATIIWDK 330
Query: 342 GSNLLSILHQLNTGTLTDGRIWLLGLNTNLLQNDALGVR*SSKRFFPLIAQVALLVCFVS 163
GSNLL I LN TLT+GR+ LL LNTNL +N++L + *+ K F P I ++ L +S
Sbjct: 329 GSNLLPIFD*LNPSTLTNGRVGLLSLNTNLFKNNSLSMG*TRKGFLPFIPKMRFLKLLIS 150
Query: 162 PPLVPAVGLELAPSSHTTCLPHG 94
PPL+ +LA SSH++CLPHG
Sbjct: 149 PPLLDTESPQLASSSHSSCLPHG 81
Score = 153 bits (328), Expect = 3e-038
Identities = 76/143 (53%), Positives = 88/143 (61%)
Frame = -3 / -1
Query: 523 KTLASPSSP*RVQAATHQTP*QP*T*HQECLPQHDPYDQIQQSTPHHSPRCSLGNHHSAQ 344
KT ASPSSP*+ Q HQ P +P*T* QE QHD Y QIQ+S HHSP+C+ NHH Q
Sbjct: 510 KT*ASPSSP*KEQVERHQKPSRP*T*LQEYHQQHDLYVQIQRSRLHHSPQCNSSNHHLGQ 331
Query: 343 RQQSSFHSSPAEHGHTYGWPNLAAWPQYQSSPERCPWREMIQQKVFSTHCPSGSSCMLCQ 164
RQQSS H E HTY W + AA PQYQS E+ P M Q++V S H S + Q
Sbjct: 330 RQQSSSHF*LIEP*HTYEWQSWAA*PQYQSFQEQFP*HGMNQKRVSSIHSQDEIS*IAYQ 151
Query: 163 PTSGSCGGS*ACAQLPYHVSSPW 95
PT G S AC QLP+ +SSPW
Sbjct: 150 PTFA*YGVSSACEQLPFLLSSPW 82
Score = 112 bits (240), Expect = 4e-026
Identities = 61/119 (51%), Positives = 73/119 (61%)
Frame = +2 / +1
Query: 95 PWGRHVVWELGASSRPTAGTRGGLTKHTRRATWAMSGKNLLLDHLTPRASFWRRLVLRPS 274
PWGR WEL AS + ++GGL + R M+G+N L H R F +RLVLR S
Sbjct: 82 PWGRQEEWELLAS*GDSVSSKGGLISNLRNLILGMNGRNPFLVHPMLRELFLKRLVLRLS 261
Query: 275 SQIRPSVSVPVFSW*RMERRLLPLCRMMVA*TTSRRMMRC*LLDLVVRVMLWETFLVSG 451
S P VSV F+ +M RRLLPL +MMVA* T RRMM+ * LDL V+VML FL SG
Sbjct: 262 SPTLPFVSVLGFN*SKMGRRLLPLSQMMVA*ITLRRMMKS*SLDLDVKVMLLVIFLESG 438
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,116,301
Number of Sequences: 7669
Number of extensions: 92212
Number of successful extensions: 1204
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 178808710
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)