TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2441599.2.1
         (755 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AJ888496.1|AJ888496  AJ888496 Medicago sativa subsp. x va...   178   7e-049

>gb|AJ888496.1|AJ888496 AJ888496 Medicago sativa subsp. x varia 4-day-old Medicago sativa
           subsp. x varia cDNA, mRNA sequence
          Length = 470

 Score =  178 bits (383), Expect(2) = 7e-049
 Identities = 72/110 (65%), Positives = 83/110 (75%)
 Frame = -2 / +1

                                                                       
Query: 637 GEVMAMDPDAVAKAFVEHYYRTFDTNRAALVGLYQETSMLTFEGQKFQGPSAIAGKLGSL 458
           G V AMDP+A++KAFVEHYY TFDTNR  L  LYQ+ SMLTFEGQ+  G   I  KL SL
Sbjct: 31  GGVRAMDPNALSKAFVEHYYTTFDTNRPNLATLYQDGSMLTFEGQQIMGSQNIVAKLTSL 210

                                                             
Query: 457 PFQACEHQIVTVDCQPSGPQGGMLVFVSGSIRTGPEEHPIKFSQAFHLLP 308
           PFQ C H I TVDCQPSG  GGMLVFVSG+++   E+H +KFSQ FHL+P
Sbjct: 211 PFQQCHHSITTVDCQPSGANGGMLVFVSGNLQLAGEQHALKFSQMFHLIP 360




 Score =  104 bits (222), Expect(2) = 2e-026
 Identities = 56/110 (50%), Positives = 62/110 (56%)
 Frame = +3 / -1

                                                                       
Query: 309 GSKWNAWENLMGCSSGPVRMEPETKTSMPPWGPDGWQSTVTI*CSQAWKGRDPSLPAMAD 488
           G KWN   NL  C S      P TKTSMPP  P+G QSTV +     WKGR+ SL  +  
Sbjct: 359 GIKWNI*LNLRACCSPASWRLPLTKTSMPPLAPEG*QSTVVME*WHCWKGREVSLATIF* 180

                                                             
Query: 489 GPWNFWPSKVSMEVSWYSPTSAARLVSNVR**CSTKAFATASGSIAITSP 638
            P    PSKVS+E SW       RLVS V **CST AF  A GSIA+T P
Sbjct: 179 DPIIC*PSKVSIEPSW*RVARLGRLVSKVV**CSTNAFDNAFGSIALTPP 30




 Score = 30.4 bits (60), Expect(2) = 7e-049
 Identities = 11/16 (68%), Positives = 14/16 (87%)
 Frame = -2 / +1

                           
Query: 301 GSFFVQNDMFRLNYG* 254
           GS++V ND+FRLNY *
Sbjct: 370 GSYYVLNDIFRLNYA* 417




 Score = 28.6 bits (56), Expect(2) = 2e-026
 Identities = 11/25 (44%), Positives = 14/25 (56%)
 Frame = +1 / -2

                                    
Query: 211 KPLNNSFITRVSAVFSHSSGGTCHS 285
           +P   +   R S  F+HSS G CHS
Sbjct: 460 RPRYANLYIRQSIXFTHSSNGICHS 386



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 5,599,195
Number of Sequences: 7669
Number of extensions: 67870
Number of successful extensions: 767
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 193632230
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)