TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441542.2.1
(3782 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO516105.1|CO516105 s13dSG65C1200098_445538 Glandular tr... 270 9e-078
gb|DQ122795.1| Medicago sativa clone M22 putative cellulose... 100 7e-043
>gb|CO516105.1|CO516105 s13dSG65C1200098_445538 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 529
Score = 270 bits (585), Expect(2) = 9e-078
Identities = 103/141 (73%), Positives = 118/141 (83%)
Frame = -1 / +1
Query: 1082 ALGSVEILFSRHCPLWYGYGGRLKFLERFAYINTTIYPLTSLPLLIYCILPAICLLTGKF 903
ALGSVEI FS+HCP+WYGYGG LK LERF+YIN+ +YP TSLPL++YC LPAICLLTGKF
Sbjct: 7 ALGSVEIFFSKHCPIWYGYGGGLKLLERFSYINSVVYPWTSLPLIVYCTLPAICLLTGKF 186
Query: 902 IIPEISNXXXXXXXXXXXXXFATGILEMRWSGVGIDEWWRNEQFWVIGGISAHLFAVFQG 723
I+PEISN ATGILEM+W GVGID+WWRNEQFWVIGG S+H FA+FQG
Sbjct: 187 IVPEISNYASLVFMALFISIAATGILEMQWGGVGIDDWWRNEQFWVIGGASSHFFALFQG 366
Query: 722 LLKVLAGIDTNFTVTSKASDE 660
LLKVLAG+DTNFTVTSKA+D+
Sbjct: 367 LLKVLAGVDTNFTVTSKAADD 429
Score = 202 bits (435), Expect(2) = 5e-058
Identities = 89/138 (64%), Positives = 101/138 (73%)
Frame = +1 / -3
Query: 670 ALEVTVKLVSMPASTLRRPWKTAKRWAEMPPMTQNCSFLHHSSMPTPLHLISRIPVAKIE 849
A +VTVK VS PA TL +PWK AK+ E PP+TQNCSFLH SS+PTP H ISRIPVA ++
Sbjct: 419 AFDVTVKFVSTPAKTLSKPWKRAKKCDEAPPITQNCSFLHQSSIPTPPHCISRIPVAAMD 240
Query: 850 MKREMNQILAKLLISGMMNFPVSRQMAGRIQ*MRSGRDVSG*MVVLM*ANLSKNFRRPP* 1029
MKR MN LA LLISG +NFPV R MAGR+Q* +GR+V G M* S NF PP*
Sbjct: 239 MKRAMNTRLA*LLISGTINFPVRRHMAGRVQ*TINGREVQGYTTEFM*EKRSNNFNPPP* 60
Query: 1030 PYHRGQCRLKRISTEPRA 1083
PY GQC LK+ISTEPRA
Sbjct: 59 PYQIGQCLLKKISTEPRA 6
Score = 39.6 bits (80), Expect(2) = 5e-058
Identities = 16/25 (64%), Positives = 17/25 (68%)
Frame = +1 / -3
Query: 562 PATTPTRLMIRMVVGGIRSVVHLNM 636
P TPT MI V+GGI S VHLNM
Sbjct: 524 PTITPTIFMINSVIGGINSEVHLNM 450
Score = 36.8 bits (74), Expect(2) = 9e-078
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = -1 / +1
Query: 662 EDGDFAELYMFKWXXXXXXXXXXXXXXLVGVVAGI 558
+DG+F+ELY+FKW +VGV+ G+
Sbjct: 424 DDGEFSELYIFKWTSLLIPPMTLLIMNIVGVIVGV 528
>gb|DQ122795.1| Medicago sativa clone M22 putative cellulose synthase catalytic
subunit mRNA, partial cds
Length = 512
Score = 99.6 bits (211), Expect(3) = 7e-043
Identities = 35/55 (63%), Positives = 48/55 (87%)
Frame = -1 / +2
Query: 830 ILEMRWSGVGIDEWWRNEQFWVIGGISAHLFAVFQGLLKVLAGIDTNFTVTSKAS 666
+LE++WSG+ + +WWRNEQFW+IGG SAH AV QGLLKV+AG+D +FT+TSK++
Sbjct: 116 LLEIKWSGITLHDWWRNEQFWLIGGTSAHPAAVLQGLLKVIAGVDISFTLTSKSA 280
Score = 78.5 bits (165), Expect(3) = 7e-043
Identities = 28/74 (37%), Positives = 41/74 (55%)
Frame = -1 / +2
Query: 665 DEDGDFAELYMFKWXXXXXXXXXXXXXXLVGVVAGISYAINSGYQSWGPLFGKLFFAFWV 486
D + +FA+LY+ KW + + G++ + S + W L G LFF+FWV
Sbjct: 290 DGEDEFADLYLVKWSFLMVPPITIMMVNTIAIAVGVARTLYSPFPQWSRLVGGLFFSFWV 469
Query: 485 IVHLYPFLKGLMGR 444
+ HLYPF KGL+GR
Sbjct: 470 LCHLYPFAKGLLGR 511
Score = 31.3 bits (62), Expect(3) = 7e-043
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -1 / +2
Query: 944 YCILPAICLLTGKFIIPEIS 885
YC LPA+ L +G+FI+ +S
Sbjct: 2 YCFLPALSLFSGQFIVQSLS 61
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 31,944,149
Number of Sequences: 7669
Number of extensions: 487626
Number of successful extensions: 2125
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 46
effective length of database: 895,794
effective search space used: 1087493916
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)