TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441110.2.2
(1019 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AJ888506.1|AJ888506 AJ888506 Medicago sativa subsp. x va... 186 2e-066
gb|CO511712.1|CO511712 s13dSG01C0100006_103324 Glandular tr... 190 5e-062
gb|CO514165.1|CO514165 s13dSG70A1000069_156992 Glandular tr... 159 5e-040
>gb|AJ888506.1|AJ888506 AJ888506 Medicago sativa subsp. x varia 4-day-old Medicago sativa
subsp. x varia cDNA, mRNA sequence
Length = 742
Score = 186 bits (400), Expect(4) = 2e-066
Identities = 76/81 (93%), Positives = 77/81 (95%)
Frame = -2 / +3
Query: 853 MFLWDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELS 674
MFL DWFYGVL SLGLWQKEAKILFLGLDNAGKTTLLHMLKD RLVQHQPTQ+PTSEELS
Sbjct: 108 MFLVDWFYGVLXSLGLWQKEAKILFLGLDNAGKTTLLHMLKDXRLVQHQPTQYPTSEELS 287
Query: 673 IGKIKFKAFDLGGHQIARRVW 611
IGKIKFKAFDLGGHQIAR VW
Sbjct: 288 IGKIKFKAFDLGGHQIARXVW 350
Score = 115 bits (245), Expect(3) = 9e-036
Identities = 52/78 (66%), Positives = 60/78 (76%)
Frame = +3 / -1
Query: 621 RAIWWPPRSNALNLILPMLSSSDVGCCVGWCCTNRSSLSMWSSVVLPALSRPRKRILASF 800
RAI PP+S ALNLI P+L+SSDVG VG C TN SLS+ S VVLPALSRP+ +ILASF
Sbjct: 340 RAI**PPKSKALNLIFPILNSSDVGY*VG*CWTNLXSLSI*SKVVLPALSRPKNKILASF 161
Query: 801 CHRPREASTP*NQSQRNI 854
CH P + TP*NQS +NI
Sbjct: 160 CHNPSDXRTP*NQSTKNI 107
Score = 64.8 bits (135), Expect(4) = 2e-066
Identities = 28/42 (66%), Positives = 29/42 (69%)
Frame = -2 / +1
Query: 535 KKEXXXXXXXXXXANVPFLILGNKIDIPYAASEEELRYHLGL 410
KKE ANVPFL+LGNKIDIPYAAS LRYHLGL
Sbjct: 427 KKELDALLSDESLANVPFLVLGNKIDIPYAASXNXLRYHLGL 552
Score = 41.8 bits (85), Expect(3) = 9e-036
Identities = 17/30 (56%), Positives = 17/30 (56%)
Frame = +1 / -1
Query: 442 RQHMGYQSCCQG*EMEHWPKNHQTGEHRAP 531
RQHM YQ C QG H P HQ GEH P
Sbjct: 520 RQHMEYQFCFQGQGKGHLPMIHQKGEHPTP 431
Score = 27.2 bits (53), Expect(4) = 2e-066
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -2 / +3
Query: 415 GLSNFTTGKGKVN 377
GL +F TGKGKVN
Sbjct: 546 GLXHFPTGKGKVN 584
Score = 26.7 bits (52), Expect(4) = 2e-066
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -3 / +1
Query: 606 ITTQRLML*YTW*MHMIRSDLLNQRR 529
IT R M TW M M R DLL+Q++
Sbjct: 355 ITMPR*MQWSTWLMLMTRKDLLSQKK 432
Score = 26.3 bits (51), Expect(3) = 9e-036
Identities = 12/24 (50%), Positives = 12/24 (50%)
Frame = +1 / -2
Query: 535 LIQQIAPYHMHLPGILQHQPLRSN 606
L QQI P H H P H P SN
Sbjct: 426 LTQQIFPCHKHQPSRPLHLPGHSN 355
>gb|CO511712.1|CO511712 s13dSG01C0100006_103324 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 532
Score = 190 bits (409), Expect(2) = 5e-062
Identities = 78/94 (82%), Positives = 83/94 (88%)
Frame = -2 / +2
Query: 553 ERFAESKKEXXXXXXXXXXANVPFLILGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNL 374
ERFAESKKE ANVPFL+LGNKIDIPYAASE+ELRYHLGLSNFTTGKGKVNL
Sbjct: 137 ERFAESKKELDALLSDESLANVPFLVLGNKIDIPYAASEDELRYHLGLSNFTTGKGKVNL 316
Query: 373 GDSNVRPLEVFMCSVVRKMGYGDGFKWVSQYIK* 272
DSNVRP+EVFMCS+V+KMGYGDGFKWVSQYIK*
Sbjct: 317 SDSNVRPMEVFMCSIVKKMGYGDGFKWVSQYIK* 418
Score = 119 bits (254), Expect(2) = 3e-032
Identities = 52/77 (67%), Positives = 56/77 (72%)
Frame = +3 / -2
Query: 273 YLMYWETHLKPSP*PILRTTLHMKTSSGRTLESPRLTLPFPVVKLLRPR*YRXXXXEAAY 452
YLMY ETHLKPSP*PI T LHM TS GRT ES + TLP PVVKLL+P+ R EAAY
Sbjct: 417 YLMY*ETHLKPSP*PIFFTILHMNTSMGRTFESDKFTLPLPVVKLLKPKW*RNSSSEAAY 238
Query: 453 GISILLPRMRNGTLAKE 503
GISIL PR R GT A +
Sbjct: 237 GISILFPRTRKGTFAND 187
Score = 111 bits (236), Expect = 2e-025
Identities = 57/128 (44%), Positives = 64/128 (50%)
Frame = +1 / -3
Query: 223 NKAKKFAGLSMRLGEGPT*CTGRPT*NHHRSPSCEQHCT*RPQVDGHWSRQG*PCPSRL* 402
NK K++ S L + *CT +P *NHH +P Q+CT* P GH S P P + *
Sbjct: 467 NKHKRYEIPSHLLLKNSI*CTEKPI*NHHHNPFSSQYCT*IPPWVGHLSLTNLPYPCQW* 288
Query: 403 SC*GLGDTVAPLQRQHMGYQSCCQG*EMEHWPKNHQTGEHRAPSLIQQIAPYHMHLPGIL 582
SC L RQHM YQ C QG H P HQ GEH P L QQI P H H P
Sbjct: 287 SCSSPNGNAIRLLRQHMEYQFCFQGQGKGHLPMIHQKGEHPTPFLTQQIFPCHKHQPSRP 108
Query: 583 QHQPLRSN 606
H P SN
Sbjct: 107 LHLPGHSN 84
Score = 71.6 bits (150), Expect(2) = 1e-027
Identities = 31/60 (51%), Positives = 39/60 (65%)
Frame = -1 / +1
Query: 614 VEGLLRKG*CCSIPGRCI**GAIC*IKEGARCSPV**FFGQCSISHPWQQD*YPICCL*R 435
+E LL G C + G C+* G IC*+K+G CSP ** G+C PW+Q+*Y ICCL R
Sbjct: 76 LERLLCPGRCSGLLG*CL*QGKIC*VKKGVGCSPF**IIGKCPFPCPWKQN*YSICCLRR 255
Score = 66.6 bits (139), Expect(2) = 1e-027
Identities = 28/63 (44%), Positives = 41/63 (65%)
Frame = -1 / +1
Query: 407 QLHNREGQGQPWRLQCPST*GLHVQCCSQDGLR*WFQVGLPVHQVGPSPRRMLSPANFFA 228
QLH+ +G G+ RL+CP+ G+HVQ C ++GL *WFQ+G VHQ+ +R L +
Sbjct: 283 QLHHWQG*GKFVRLKCPTHGGIHVQYCEENGLW*WFQMGFSVHQIEFFSKRWLGISYLLC 462
Query: 227 LFV 219
LF+
Sbjct: 463 LFL 471
Score = 62.9 bits (131), Expect(2) = 5e-062
Identities = 25/25 (100%), Positives = 25/25 (100%)
Frame = -2 / +2
Query: 685 EELSIGKIKFKAFDLGGHQIARRVW 611
EELSIGKIKFKAFDLGGHQIARRVW
Sbjct: 5 EELSIGKIKFKAFDLGGHQIARRVW 79
Score = 34.1 bits (68), Expect(2) = 3e-032
Identities = 15/22 (68%), Positives = 18/22 (81%)
Frame = +3 / -2
Query: 621 RAIWWPPRSNALNLILPMLSSS 686
RAI PP+S ALNLI P+L+SS
Sbjct: 69 RAI**PPKSKALNLIFPILNSS 4
>gb|CO514165.1|CO514165 s13dSG70A1000069_156992 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 359
Score = 159 bits (342), Expect = 5e-040
Identities = 65/69 (94%), Positives = 68/69 (98%)
Frame = -2 / +3
Query: 853 MFLWDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELS 674
MF++D FYG+LASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELS
Sbjct: 153 MFIFDGFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELS 332
Query: 673 IGKIKFKAF 647
IGKIKFKAF
Sbjct: 333 IGKIKFKAF 359
Score = 105 bits (223), Expect = 1e-023
Identities = 47/68 (69%), Positives = 54/68 (79%)
Frame = +3 / -2
Query: 651 ALNLILPMLSSSDVGCCVGWCCTNRSSLSMWSSVVLPALSRPRKRILASFCHRPREASTP 830
ALNLI P+L+SS+VGC VG C TN SSLS+ VV PALSRPRKRILASFC RPR+A P
Sbjct: 355 ALNLIFPILNSSEVGCWVG*C*TNLSSLSI*RRVVFPALSRPRKRILASFCQRPRDARMP 176
Query: 831 *NQSQRNI 854
*N S+ N+
Sbjct: 175 *NPSKMNM 152
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,007,047
Number of Sequences: 7669
Number of extensions: 87147
Number of successful extensions: 756
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 271965096
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)