TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440806.2.2
(1155 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U41472.1|MSU41472 Medicago sativa pectate lyase homolog ... 201 1e-092
>gb|U41472.1|MSU41472 Medicago sativa pectate lyase homolog gene, complete cds
Length = 3824
Score = 201 bits (434), Expect(4) = 1e-092
Identities = 77/115 (66%), Positives = 93/115 (80%)
Frame = +2 / +3
Query: 395 VMLFGASNDSPQDAVMQVTVAFNHFGRGLVQRMPRCRYGFFHVVNNDYTHWIMYAIGGNM 574
VMLFGAS+ D +MQ+TVAFNHFG+GL+QRMPRCR+GFFHV+NNDYTHWIMYAIGG+
Sbjct: 2862 VMLFGASDTYQDDKIMQITVAFNHFGQGLIQRMPRCRWGFFHVLNNDYTHWIMYAIGGSS 3041
Query: 575 NPTIISQGNRFIAPDDPNAKEVTKREYTPYKDYKEWVWKSQGDVMMNGAFFNESG 739
PTI+SQGNRFIAP + AK VT R+Y P + +W W+S+GD MNGA F +SG
Sbjct: 3042 APTILSQGNRFIAPHNNAAKTVTHRDYAPESVWSKWQWRSEGDHFMNGATFIQSG 3206
Score = 121 bits (259), Expect(4) = 3e-045
Identities = 62/115 (53%), Positives = 69/115 (60%)
Frame = -1 / -2
Query: 738 PDSLKKAPFIMTSPCDFHTHSL*SLYGVYSRLVTSLALGSSGAMKRLPWLMMVGFMLPPM 559
PD + APF+ SP D H H + G S VT A GAMKR P L +VG LPP+
Sbjct: 3205 PD*INVAPFMK*SPSDLHCHFDQTDSGA*SLCVTVFAALL*GAMKRFP*LRIVGAELPPI 3026
Query: 558 AYMIQCV*SLFTTWKKP*RQRGIRCTSPRPKWLNATVTCITASCGLSLLAPNSIT 394
AYMIQCV*SLF TWK P GI +P PKWLNATV CI S LAPN+IT
Sbjct: 3025 AYMIQCV*SLFKTWKNPHLHLGIL*INPCPKWLNATVICIILSS*YVSLAPNNIT 2861
Score = 101 bits (216), Expect(4) = 1e-092
Identities = 37/73 (50%), Positives = 49/73 (67%)
Frame = +2 / +2
Query: 185 KAHSGGMIRDSKRHYGLRTRXXXXXXXXXXXXNVWIDHVSMSSCSDGLIDVVNGSTAITV 364
KA +GG+IRDS H G+RTR N+WIDH+S+S C DGL+DV+ GSTA+T+
Sbjct: 2510 KARNGGLIRDSFDHIGVRTRSDGDAISVFGSSNIWIDHISLSDCEDGLVDVIQGSTAVTI 2689
Query: 365 SNSHFTDHDHVML 403
SN H T H+ V +
Sbjct: 2690 SNCHMTKHNDVSI 2728
Score = 59.3 bits (123), Expect(4) = 1e-092
Identities = 24/35 (68%), Positives = 28/35 (80%)
Frame = +2 / +2
Query: 11 PLWIVFARDMVIELRQELIVNHNKTIDGRGAQVHI 115
PLWI+F R MVI L QEL+V+ +KTIDGRGA V I
Sbjct: 2333 PLWIIFQRSMVITLTQELMVSSDKTIDGRGANVQI 2437
Score = 51.5 bits (106), Expect(4) = 3e-045
Identities = 29/66 (43%), Positives = 34/66 (51%)
Frame = -1 / -3
Query: 381 VKWLLDTVMAVDPFTTSISPSEQLDMDTWSIHTLLDDSTETPSPSLRVRSP*CRFESRIM 202
V W + V AV+P TS PS Q D + SI + TE SPSL V +P ES I
Sbjct: 2706 VMWQFEMVTAVEP*MTSTRPSSQSDKEI*SIQIFDEPKTEIASPSLLVLTPI*SKESLIK 2527
Query: 201 PPECAL 184
PP AL
Sbjct: 2526 PPFLAL 2509
Score = 43.7 bits (89), Expect(4) = 1e-092
Identities = 17/40 (42%), Positives = 24/40 (60%)
Frame = +2 / +3
Query: 785 KHGRYVGQLTRFAGPLKCIVGQPC**TAARSGTYSSSYIH 904
+HG +LTRF+G L C+VG+PC* YS Y++
Sbjct: 3255 RHGSQANRLTRFSGALNCVVGRPC*KNK*IPKVYSLMYLY 3374
Score = 36.8 bits (74), Expect(4) = 3e-045
Identities = 18/37 (48%), Positives = 25/37 (67%)
Frame = -1 / -3
Query: 120 NMMCTCAPRPSIVLLWFTISSCRSSITMSRANTIHSG 10
+++CT APRPS+VL TI+S + +TM R I SG
Sbjct: 2442 SLICTLAPRPSMVLSDETINS*VNVMTMLRWKIIQSG 2332
Score = 36.3 bits (73), Expect(4) = 3e-045
Identities = 18/40 (45%), Positives = 22/40 (55%)
Frame = -1 / -2
Query: 888 EYVPDRAAVY*HGWPTMHLSGPAKRVS*PT*RPCLAGMKS 769
EY +*HG PT LS P KRVS * PCL +++
Sbjct: 3358 EYTLGIYLFF*HGLPTTQLSAPEKRVSLLA*DPCLGFIRN 3239
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,858,046
Number of Sequences: 7669
Number of extensions: 85314
Number of successful extensions: 601
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 313311141
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)