TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440806.2.1
(1136 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U41472.1|MSU41472 Medicago sativa pectate lyase homolog ... 191 2e-083
>gb|U41472.1|MSU41472 Medicago sativa pectate lyase homolog gene, complete cds
Length = 3824
Score = 191 bits (411), Expect(4) = 2e-083
Identities = 75/115 (65%), Positives = 89/115 (77%)
Frame = -3 / +3
Query: 777 VMLFGASDAASKDREMQVTVAFNHFGKGLVQRMPRCRHGFFHVVNNDYTHWLMYAIGGSR 598
VMLFGASD D+ MQ+TVAFNHFG+GL+QRMPRCR GFFHV+NNDYTHW+MYAIGGS
Sbjct: 2862 VMLFGASDTYQDDKIMQITVAFNHFGQGLIQRMPRCRWGFFHVLNNDYTHWIMYAIGGSS 3041
Query: 597 NPTIISQGNRFRAVDDSRFKEVTKREYTQYSEYKNWVWKSQDDLFLNGAFFNQSG 433
PTI+SQGNRF A ++ K VT R+Y S + W W+S+ D F+NGA F QSG
Sbjct: 3042 APTILSQGNRFIAPHNNAAKTVTHRDYAPESVWSKWQWRSEGDHFMNGATFIQSG 3206
Score = 101 bits (215), Expect(4) = 2e-083
Identities = 43/95 (45%), Positives = 54/95 (56%)
Frame = -3 / +2
Query: 1053 GAQITLQNVRNVILHNLXXXXXXXXXXXAIRDSQHHWGVRXXXXXXXXXXXXXXDIWIDH 874
GA IT+Q V NVI+H L IRDS H GVR +IWIDH
Sbjct: 2444 GAGITMQFVNNVIIHGLRIKNIKARNGGLIRDSFDHIGVRTRSDGDAISVFGSSNIWIDH 2623
Query: 873 LSMSSCADGLVDAVDGSTAITVSNGHFTRHDHVML 769
+S+S C DGLVD + GSTA+T+SN H T+H+ V +
Sbjct: 2624 ISLSDCEDGLVDVIQGSTAVTISNCHMTKHNDVSI 2728
Score = 98.7 bits (209), Expect(4) = 5e-040
Identities = 49/86 (56%), Positives = 53/86 (61%)
Frame = +2 / -2
Query: 521 SRLVTSLNLLSSTARKRLPWLMMVGFRLPPMAYMSQCV*SLFTTWKKP*RQRGIRCTSPL 700
S VT L A KR P L +VG LPP+AYM QCV*SLF TWK P GI +P
Sbjct: 3118 SLCVTVFAALL*GAMKRFP*LRIVGAELPPIAYMIQCV*SLFKTWKNPHLHLGIL*INPC 2939
Query: 701 PKWLKATVTCISLSLDAASLAPNSIT 778
PKWL ATV CI LS SLAPN+IT
Sbjct: 2938 PKWLNATVICIILSS*YVSLAPNNIT 2861
Score = 70.7 bits (148), Expect(4) = 5e-040
Identities = 44/96 (45%), Positives = 51/96 (53%)
Frame = +2 / -3
Query: 773 ITWSCLVKWPLETVMAVEPSTASTSPSAQLLMDRWSIQISLDPITETPSPSLSPRTPQWC 952
+T CLV W E V AVEP ST PS+Q + SIQI +P TE SPSL TP
Sbjct: 2724 LTSLCLVMWQFEMVTAVEP*MTSTRPSSQSDKEI*SIQIFDEPKTEIASPSLLVLTPI*S 2545
Query: 953 CESRIAPPPCAAASWTCRLWRITLRTFCSVICAPTM 1060
ES I PP A R +TL T C VI AP++
Sbjct: 2544 KESLIKPPFLALMFLMRRP*MMTLFTNCMVIPAPSL 2437
Score = 42.8 bits (87), Expect(4) = 2e-083
Identities = 18/24 (75%), Positives = 20/24 (83%)
Frame = -3 / +2
Query: 1128 VELCQELIVSSDKTIDGRGAQVHI 1057
+ L QEL+VSSDKTIDGRGA V I
Sbjct: 2366 ITLTQELMVSSDKTIDGRGANVQI 2437
Score = 40.0 bits (81), Expect(4) = 2e-083
Identities = 16/29 (55%), Positives = 21/29 (72%)
Frame = -3 / +3
Query: 399 LIQAKGGQYAESLTRYAGALNCRVGRKC* 313
L++ + G A LTR++GALNC VGR C*
Sbjct: 3243 LMKPRHGSQANRLTRFSGALNCVVGRPC* 3329
Score = 31.3 bits (62), Expect(4) = 5e-040
Identities = 13/19 (68%), Positives = 16/19 (84%)
Frame = +2 / -3
Query: 1058 MCTCAPRPSMVLSLLTMSS 1114
+CT APRPSMVLS T++S
Sbjct: 2436 ICTLAPRPSMVLSDETINS 2380
Score = 27.6 bits (54), Expect(4) = 5e-040
Identities = 16/30 (53%), Positives = 19/30 (63%)
Frame = +2 / -2
Query: 314 *HFLPTRQLSAPAYLVSDSAYWPPLAWMRS 403
*H LPT QLSAP VS A P L ++R+
Sbjct: 3328 *HGLPTTQLSAPEKRVSLLA*DPCLGFIRN 3239
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,166,896
Number of Sequences: 7669
Number of extensions: 95797
Number of successful extensions: 854
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 307798335
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)