TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440570.2.2
(905 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY205233.1| Medicago sativa 60S ribosomal protein mRNA, ... 314 7e-087
>gb|AY205233.1| Medicago sativa 60S ribosomal protein mRNA, partial cds
Length = 544
Score = 314 bits (681), Expect = 7e-087
Identities = 130/163 (79%), Positives = 149/163 (91%)
Frame = +2 / +1
Query: 86 MGIDLVAGGRNKKTKRTAPKSDDVYLKLLVKLYRFLVRRTKSNFNAVILKRLFMSKTNRP 265
MGIDL AGG++KKTKRTAPKS+D+YLKLLVKLYRFLVRRT+SNFN VILKRLFMSK N+P
Sbjct: 52 MGIDLKAGGKSKKTKRTAPKSNDIYLKLLVKLYRFLVRRTESNFNKVILKRLFMSKVNKP 231
Query: 266 PISMRRLVKFMEGKEKNIAVIVGTVTDDKRIQEVPAMKVTALRFTETARARIVNAGGECL 445
P+S+ RL+++ +GKE IAV+VG VTDD R+ EVPA+KVTALRFTE ARARI AGGECL
Sbjct: 232 PLSLSRLIRYTKGKEGKIAVVVGAVTDDIRVYEVPAIKVTALRFTEKARARIEKAGGECL 411
Query: 446 TFDQLALRAPLGENTVLLRGPKNAREAVRHFGKAPGVPHSHTK 574
TFDQLAL+APLG+NTVLLRGPKNAREAV+HFG+APGVPHS TK
Sbjct: 412 TFDQLALKAPLGQNTVLLRGPKNAREAVKHFGRAPGVPHSSTK 540
Score = 166 bits (356), Expect(2) = 9e-047
Identities = 77/110 (70%), Positives = 83/110 (75%)
Frame = -3 / -3
Query: 573 LVWLCGTPGALPKCLTASRAFLGPLKRTVFSPSGARRASWSNVRHSPPALTILALAVSVN 394
LV LCGTPGA PKCLTASRAFLGPL+RTVF P GA RAS S VRHSPPA +ILALA SVN
Sbjct: 539 LVLLCGTPGARPKCLTASRAFLGPLRRTVFCPKGALRAS*SKVRHSPPAFSILALAFSVN 360
Query: 393 LRAVTFIAGTSWILLSSVTVPTMTAMFFSFPSINLTRRRMEIGGRLVLLM 244
LRAVT IAGTS L+SSVT PT TA+ SFP + + GG L LLM
Sbjct: 359 LRAVTLIAGTS*TLMSSVTAPTTTAILPSFPLV*RINLESDSGGLLTLLM 210
Score = 109 bits (232), Expect = 6e-025
Identities = 54/125 (43%), Positives = 73/125 (58%)
Frame = -2 / -2
Query: 574 LGVAVRHSRSLAKVPHCLTGILGAPQEDRVLAKWSTKSKLVKCEALATSIDNPGPCCLRE 395
L AV ++RS +KV LTGI G +++RVL S KS+L+K EAL TS+ N P E
Sbjct: 540 LSAAVWYARSSSKVLDSLTGIFGTSKKNRVLP*RSFKSQLIKGEALPTSLLNSSPGLFGE 361
Query: 394 PQGSNLHCWNLLDPFVICDCANNDSNVLLLSFHKLDKAAHGDWWSVGFTHEKPLENDSVE 215
P S L+C ++P VIC NN N+ LLS ++ WW V F HEKPL+++ +E
Sbjct: 360 P*SSYLNCRYFINPNVICHGTNNYGNLTLLSLGVTNQPRK**WWLVDFAHEKPLKDNLIE 181
Query: 214 IALGP 200
I P
Sbjct: 180 ITFSP 166
Score = 103 bits (219), Expect = 4e-023
Identities = 55/138 (39%), Positives = 71/138 (51%)
Frame = +1 / +3
Query: 157 LPQAPRQALPFLGQEDQEQFQRCHSQEAFHE*NQPTTNLHAPPCQVYGRKGEEHCCHCWH 336
LPQA R A+PF +D + FQ + EAFHE +Q T + ++ KG C CW
Sbjct: 123 LPQASR*AIPFPCSKD*K*FQ*GYP*EAFHEQSQQATTITF*VDSLHQGKGG*DCRSCWC 302
Query: 337 SHR*QKDPGGSSNEGYCPEVHGDSKGQDCQCWWRVPHI*PACSSCSTWREHGPLEGPQEC 516
R* + N G C +VH +G + + WW VPH+* A S S EHG Q+C
Sbjct: 303 RDR*H*GL*STCN*GNCSKVHRKGQG*N*EGWWGVPHL*SAGS*SSFRAEHGSS*RSQKC 482
Query: 517 P*GSEALWQGSWSAAQPH 570
P*G +ALW SW Q H
Sbjct: 483 P*GCQALWTSSWRTTQQH 536
Score = 35.9 bits (72), Expect(2) = 9e-047
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = -3 / -3
Query: 141 GAVRLVFLFLPPATRSIPI 85
GAVRLVFL LPPA +SIP+
Sbjct: 107 GAVRLVFLDLPPAFKSIPM 51
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,282,948
Number of Sequences: 7669
Number of extensions: 127360
Number of successful extensions: 1509
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 237050658
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)