TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2437779.2.1
(3727 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO516105.1|CO516105 s13dSG65C1200098_445538 Glandular tr... 281 2e-080
gb|DQ122795.1| Medicago sativa clone M22 putative cellulose... 129 5e-048
>gb|CO516105.1|CO516105 s13dSG65C1200098_445538 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 529
Score = 281 bits (608), Expect(2) = 2e-080
Identities = 106/141 (75%), Positives = 127/141 (90%)
Frame = -2 / +1
Query: 1020 ALGSIEILFSRHCPIWYGYGGRLKFLERFAYINTTIYPLTSIPLLLYCILPAVCLLTGKF 841
ALGS+EI FS+HCPIWYGYGG LK LERF+YIN+ +YP TS+PL++YC LPA+CLLTGKF
Sbjct: 7 ALGSVEIFFSKHCPIWYGYGGGLKLLERFSYINSVVYPWTSLPLIVYCTLPAICLLTGKF 186
Query: 840 IIPKISNLESVWFISLFISIFATGILEMRWSGVGIDEWWRNEQFWVIGGISAHLFAVFQG 661
I+P+ISN S+ F++LFISI ATGILEM+W GVGID+WWRNEQFWVIGG S+H FA+FQG
Sbjct: 187 IVPEISNYASLVFMALFISIAATGILEMQWGGVGIDDWWRNEQFWVIGGASSHFFALFQG 366
Query: 660 LLKVLAGIDTSFTVTSKATDE 598
LLKVLAG+DT+FTVTSKA D+
Sbjct: 367 LLKVLAGVDTNFTVTSKAADD 429
Score = 182 bits (393), Expect(2) = 4e-052
Identities = 79/138 (57%), Positives = 96/138 (69%)
Frame = +2 / -3
Query: 608 ALEVTVKLVSIPASTFRRPWKTANKCAEIPPMTQNCSFLHHSSMPTPLHLISRIPVAKIE 787
A +VTVK VS PA T +PWK A KC E PP+TQNCSFLH SS+PTP H ISRIPVA ++
Sbjct: 419 AFDVTVKFVSTPAKTLSKPWKRAKKCDEAPPITQNCSFLHQSSIPTPPHCISRIPVAAMD 240
Query: 788 IKSDINQTLSRLLIFGMMNFPVRRQTAGNMQYRRSGIDVSG*IVVLI*ANLSRNLSRPP* 967
+K +N L+ LLI G +NFPVRR AG +Q +G +V G +* S N + PP*
Sbjct: 239 MKRAMNTRLA*LLISGTINFPVRRHMAGRVQ*TINGREVQGYTTEFM*EKRSNNFNPPP* 60
Query: 968 PYHMGQCLLKRISMEPRA 1021
PY +GQCLLK+IS EPRA
Sbjct: 59 PYQIGQCLLKKISTEPRA 6
Score = 77.1 bits (162), Expect(3) = 4e-025
Identities = 37/81 (45%), Positives = 53/81 (65%)
Frame = +1 / -2
Query: 619 DSEARVDTSKHLQETLEDGK*MRRNTTNDPELLVPPPFINANTTPPHLKDTSGKD*DKER 798
+SE VDTS +L++TLE GK M +T+N P+L +PPP I +NTTP HLKDT D +E
Sbjct: 408 NSEVCVDTS*NLEQTLEKGKKM**STSNHPKLFIPPPIIYSNTTPLHLKDTGCCDGYEEG 229
Query: 799 YKPNTL*VTNLWDDELPSEKT 861
++ T V L +++ +KT
Sbjct: 228 HEHKTCIVAYLRNNKFSGQKT 166
Score = 46.9 bits (96), Expect(3) = 4e-025
Identities = 17/33 (51%), Positives = 25/33 (75%)
Frame = +1 / -2
Query: 928 ISKSLQEFKPTSIAIPYGTMPAEKNFNGTKSPP 1026
+ K+ Q+ +PT++A+P TM AEK+ N TKSPP
Sbjct: 99 VGKTFQQLQPTTVAVPNWTMFAEKDLNRTKSPP 1
Score = 39.1 bits (79), Expect(2) = 4e-052
Identities = 16/25 (64%), Positives = 17/25 (68%)
Frame = +2 / -3
Query: 500 PATTPTRLMIKIVVGGIRSVVHLNM 574
P TPT MI V+GGI S VHLNM
Sbjct: 524 PTITPTIFMINSVIGGINSEVHLNM 450
Score = 35.0 bits (70), Expect(2) = 2e-080
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = -2 / +1
Query: 600 EEGDFAELYMFKWXXXXXXXXXXXXXXLVGVVAGI 496
++G+F+ELY+FKW +VGV+ G+
Sbjct: 424 DDGEFSELYIFKWTSLLIPPMTLLIMNIVGVIVGV 528
Score = 24.9 bits (48), Expect(3) = 4e-025
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +1 / -2
Query: 496 NASDHADQVDDQNSGWWDQKRCPLEHVEL 582
+ ++HA + D W D R P E+VEL
Sbjct: 528 DTNNHAHNIHD**RHWRD**RSPFEYVEL 442
>gb|DQ122795.1| Medicago sativa clone M22 putative cellulose synthase catalytic
subunit mRNA, partial cds
Length = 512
Score = 129 bits (277), Expect(2) = 5e-048
Identities = 48/96 (50%), Positives = 70/96 (72%)
Frame = -2 / +2
Query: 882 YCILPAVCLLTGKFIIPKISNLESVWFISLFISIFATGILEMRWSGVGIDEWWRNEQFWV 703
YC LPA+ L +G+FI+ +S V+ + + +++ +LE++WSG+ + +WWRNEQFW+
Sbjct: 2 YCFLPALSLFSGQFIVQSLSVTFLVFLLGITVTLCLLALLEIKWSGITLHDWWRNEQFWL 181
Query: 702 IGGISAHLFAVFQGLLKVLAGIDTSFTVTSKATDEE 595
IGG SAH AV QGLLKV+AG+D SFT+TSK+ E
Sbjct: 182 IGGTSAHPAAVLQGLLKVIAGVDISFTLTSKSATPE 289
Score = 78.5 bits (165), Expect(2) = 5e-048
Identities = 28/74 (37%), Positives = 41/74 (55%)
Frame = -2 / +2
Query: 603 DEEGDFAELYMFKWXXXXXXXXXXXXXXLVGVVAGISYAINSGYQSWGPLFGKLFFAFWV 424
D E +FA+LY+ KW + + G++ + S + W L G LFF+FWV
Sbjct: 290 DGEDEFADLYLVKWSFLMVPPITIMMVNTIAIAVGVARTLYSPFPQWSRLVGGLFFSFWV 469
Query: 423 IVHLYPFLKGLMGK 382
+ HLYPF KGL+G+
Sbjct: 470 LCHLYPFAKGLLGR 511
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 33,007,746
Number of Sequences: 7669
Number of extensions: 522902
Number of successful extensions: 3474
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 46
effective length of database: 895,794
effective search space used: 1070473830
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)