TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2430946.2.3
(3896 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO516105.1|CO516105 s13dSG65C1200098_445538 Glandular tr... 281 3e-090
gb|DQ122795.1| Medicago sativa clone M22 putative cellulose... 127 1e-053
>gb|CO516105.1|CO516105 s13dSG65C1200098_445538 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 529
Score = 281 bits (607), Expect(2) = 3e-090
Identities = 108/141 (76%), Positives = 124/141 (87%)
Frame = -2 / +1
Query: 1273 ALGSVEILLSRHCPIWYGYNGRLKLLERLAYINTIVYPITSVPLIAYCVLPAICLLTNKF 1094
ALGSVEI S+HCPIWYGY G LKLLER +YIN++VYP TS+PLI YC LPAICLLT KF
Sbjct: 7 ALGSVEIFFSKHCPIWYGYGGGLKLLERFSYINSVVYPWTSLPLIVYCTLPAICLLTGKF 186
Query: 1093 IIPEISNYAGMFFILLFASIFATGILELRWSGVGIEDWWRNEQFWVIGGTSAHLFAVFQG 914
I+PEISNYA + F+ LF SI ATGILE++W GVGI+DWWRNEQFWVIGG S+H FA+FQG
Sbjct: 187 IVPEISNYASLVFMALFISIAATGILEMQWGGVGIDDWWRNEQFWVIGGASSHFFALFQG 366
Query: 913 LLKVLAGIDTNFTVTSKASDE 851
LLKVLAG+DTNFTVTSKA+D+
Sbjct: 367 LLKVLAGVDTNFTVTSKAADD 429
Score = 188 bits (406), Expect(2) = 4e-051
Identities = 86/138 (62%), Positives = 97/138 (70%)
Frame = +3 / -3
Query: 861 AFEVTVKLVSIPANTFSRPWNTAKRWAEVPPITQNCSFLHQSSMPTPLHLSSNIPVAKME 1040
AF+VTVK VS PA T S+PW AK+ E PPITQNCSFLHQSS+PTP H S IPVA M+
Sbjct: 419 AFDVTVKFVSTPAKTLSKPWKRAKKCDEAPPITQNCSFLHQSSIPTPPHCISRIPVAAMD 240
Query: 1041 AKRRMKNIPA*LLISGMINLLVRRQIAGSTQ*AISGTDVIG*TMVLM*ASLSKSFNRPL* 1220
KR M A*LLISG IN VRR +AG Q* I+G +V G T M* S +FN P *
Sbjct: 239 MKRAMNTRLA*LLISGTINFPVRRHMAGRVQ*TINGREVQGYTTEFM*EKRSNNFNPPP* 60
Query: 1221 PYHIGQCLLSRISTDPRA 1274
PY IGQCLL +IST+PRA
Sbjct: 59 PYQIGQCLLKKISTEPRA 6
Score = 68.4 bits (143), Expect(2) = 3e-090
Identities = 22/35 (62%), Positives = 33/35 (94%)
Frame = -2 / +1
Query: 853 EDGDFAELYVFKWTSLLIPPTTVLVINLVGMVAGI 749
+DG+F+ELY+FKWTSLLIPP T+L++N+VG++ G+
Sbjct: 424 DDGEFSELYIFKWTSLLIPPMTLLIMNIVGVIVGV 528
Score = 29.9 bits (59), Expect(2) = 4e-051
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +3 / -3
Query: 753 PATIPTRLMTRTVVGGMSKLVHLN 824
P PT M +V+GG++ VHLN
Sbjct: 524 PTITPTIFMINSVIGGINSEVHLN 453
>gb|DQ122795.1| Medicago sativa clone M22 putative cellulose synthase catalytic
subunit mRNA, partial cds
Length = 512
Score = 127 bits (271), Expect(2) = 1e-053
Identities = 47/93 (50%), Positives = 69/93 (74%)
Frame = -2 / +2
Query: 1135 YCVLPAICLLTNKFIIPEISNYAGMFFILLFASIFATGILELRWSGVGIEDWWRNEQFWV 956
YC LPA+ L + +FI+ +S +F + + ++ +LE++WSG+ + DWWRNEQFW+
Sbjct: 2 YCFLPALSLFSGQFIVQSLSVTFLVFLLGITVTLCLLALLEIKWSGITLHDWWRNEQFWL 181
Query: 955 IGGTSAHLFAVFQGLLKVLAGIDTNFTVTSKAS 857
IGGTSAH AV QGLLKV+AG+D +FT+TSK++
Sbjct: 182 IGGTSAHPAAVLQGLLKVIAGVDISFTLTSKSA 280
Score = 100 bits (213), Expect(2) = 1e-053
Identities = 33/74 (44%), Positives = 52/74 (70%)
Frame = -2 / +2
Query: 856 DEDGDFAELYVFKWTSLLIPPTTVLVINLVGMVAGISYAINSGYQSWGPLFGKLFFSIWV 677
D + +FA+LY+ KW+ L++PP T++++N + + G++ + S + W L G LFFS WV
Sbjct: 290 DGEDEFADLYLVKWSFLMVPPITIMMVNTIAIAVGVARTLYSPFPQWSRLVGGLFFSFWV 469
Query: 676 ILHLYPFLKGLMGR 635
+ HLYPF KGL+GR
Sbjct: 470 LCHLYPFAKGLLGR 511
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 34,926,103
Number of Sequences: 7669
Number of extensions: 519531
Number of successful extensions: 2532
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 46
effective length of database: 895,794
effective search space used: 1121534088
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)